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1. 发明申请

WO2003100044A1 NEW IMPROVED ACYLTRANSFERASE 审中-公开
标题翻译：新改进的ACYLTRANSFERASE
公开(公告)号：WO2003100044A1
公开(公告)日：2003-12-04
申请号：PCT/SE2003/000870
申请日：2003-05-28
申请人： SCANDINAVIAN BIOTECHNOLOGY RESEARCH (ScanBi) AB , DAHLQVIST, Anders , GHOSAL, Alokesh , LINDQVIST, Ylva , BANAS, Antoni
发明人： DAHLQVIST, Anders , GHOSAL, Alokesh , LINDQVIST, Ylva , BANAS, Antoni
IPC分类号： C12N9/10
CPC分类号： C12N9/1029 , Y10S435/81
摘要： The invention relates to at least one nucleotide sequence, derived from a nucleotide sequence encoding an acyltransferase polypeptide comprising at least one membrane-spanning region, encoding an improved active membrane independent acyltransferase polypeptide in which at least one amino acid residue of the membrane-spanning region has been deleted and/or substituted as compared to the original acyltransferase polypeptide, wherein the encoded active membrane independent acyltransferase polypeptide, wherein the encoded active membrane independent acyltransferase polypeptide can produce fatty acid esters and/or fatty acid thioesters such as triacylglycerols, diacylglycerols, monoacylglycerols, phospholipids, glycolipids, waxesters, acylated carbohydrates, acylated amino acids, and lysolipids, e.g. lysophospholipid, lysolecithin. Thereby one single acyltransferase can be used for the production of a huge number of products. The invention also relates to means and methods for the production of such an improved active membrane independent acyltransferase and the use of such a membrane independent acyltransferase in industry.
摘要翻译：本发明涉及源于编码包含至少一个跨膜区的酰基转移酶多肽的核苷酸序列的至少一个核苷酸序列，其编码改进的活性膜独立的酰基转移酶多肽，其中所述跨膜区的至少一个氨基酸残基与原始酰基转移酶多肽相比已经被删除和/或取代，其中编码的活性膜独立的酰基转移酶多肽，其中所编码的活性膜独立的酰基转移酶多肽可以产生脂肪酸酯和/或脂肪酸硫酯如三酰基甘油，二酰基甘油，单酰基甘油，磷脂，糖脂，蜡质酯，酰化碳水化合物，酰化氨基酸和溶脂质，例如溶血磷脂，溶血卵磷脂。因此，单个酰基转移酶可用于生产大量产品。本发明还涉及用于生产这种改进的活性膜独立酰基转移酶的方法和方法，以及在工业中使用这种不依赖于膜的酰基转移酶。

2. 发明申请

WO0060095A3 ENZYMES OF THE BIOSYNTHETIC PATHWAY FOR THE PRODUCTION OF TRIACYLGLYCEROL AND RECOMBINANT DNA MOLECULES ENCODING THESE ENZYMES 审中-公开
标题翻译：用于生产三乙基胆碱的生物路径的酶和编码这些酶的重组DNA分子
公开(公告)号：WO0060095A3
公开(公告)日：2001-02-01
申请号：PCT/EP0002701
申请日：2000-03-28
申请人： BASF PLANT SCIENCE GMBH , DAHLQVIST ANDERS , STAHL ULF , LENMAN MARIT , BANAS ANTONI , RONNE HANS , STYMNE STEN
发明人： DAHLQVIST ANDERS , STAHL ULF , LENMAN MARIT , BANAS ANTONI , RONNE HANS , STYMNE STEN
IPC分类号： A01H5/00 , A01K67/027 , C12N1/16 , C12N1/19 , C12N5/10 , C12N9/10 , C12N15/09 , C12N15/54 , C12N15/81 , C12N15/82 , C12P7/64 , C12R1/645
CPC分类号： C12N9/1029 , A01K2217/05
摘要： The present invention relates to the isolation, identification and characterization of nucleotide sequences encoding an enzyme catalysing the transfer of fatty acids from phospholipids to diacylglycerol in the biosynthetic pathway for the production of triacylglycerol, to the said enzymes and a process for the production of triacylglycerols.
摘要翻译：本发明涉及编码催化脂肪酸从磷脂向二酰基甘油转移到生产三酰基甘油的生物合成途径中的酶的所述酶的编码酶的核苷酸序列的分离，鉴定和表征以及三酰基甘油的制备方法。

3. 发明申请

WO2003083100A1 EXPRESSION VON PHOSPHOLIPID: DIACYLGLYCERIN-ACYLTRANSFERASE (PDAT) ZUR HERSTELLUNG VON PLANZLICHEN SPEICHERLIPIDEN ENTHALTEND MEHRFACH UNGESÄTTIGTE FETTSÄUREN 审中-公开
标题翻译：表达的磷脂：二酰基甘油酰基转移酶（PDAT）FOR MEMORY PLANZLICHEN脂质产生含有多不饱和
公开(公告)号：WO2003083100A1
公开(公告)日：2003-10-09
申请号：PCT/EP2003/002955
申请日：2003-03-21
申请人： BASF PLANT SCIENCE GMBH , STYMNE, Sten , LENMAN, Marit , STAHL, Ulf , BANAS, Antoni , CARLSSON, Anders , WIBERG, Eva , DAHLQVIST, Anders
发明人： STYMNE, Sten , LENMAN, Marit , STAHL, Ulf , BANAS, Antoni , CARLSSON, Anders , WIBERG, Eva , DAHLQVIST, Anders
IPC分类号： C12N9/10
CPC分类号： C12N9/1029 , C12N15/8247
摘要： Die vorliegende Erfindung betrifft die Expression wenigstens eines Enzyms mit Phospholipid: Diacylglycerin-Acyltransferase Aktivität zur Herstellung von pflanzlichen Speicherlipiden enthaltend mehrfach ungesättigte Fettsäuren.
摘要翻译：本发明涉及具有磷脂至少一种酶的表达：用于生产含多不饱和脂肪酸的植物贮藏脂质二酰基甘油的酰基转移酶活性。

4. 发明申请

WO2004007727A1 USE OF A GENE FOR INCREASING THE OIL CONTENT IN PLANTS 审中-公开
标题翻译：使用基因增加植物油含量
公开(公告)号：WO2004007727A1
公开(公告)日：2004-01-22
申请号：PCT/EP2003/007084
申请日：2003-07-03
申请人： BASF PLANT SCIENCE GMBH , GIPMANS, Martijn , DAHLQVIST, Anders , BANAS, Antoni , STÄHL, Ulf , WIBERG, Eva , LENMAN, Marit , RONNE, Hans , STYMNE, Sten
发明人： GIPMANS, Martijn , DAHLQVIST, Anders , BANAS, Antoni , STÄHL, Ulf , WIBERG, Eva , LENMAN, Marit , RONNE, Hans , STYMNE, Sten
IPC分类号： C12N15/52
CPC分类号： C12N15/8247 , C07K14/395
摘要： The invention relates to methods for increasing the oil content in plants, preferably in plant seeds, by expressing a polypeptide from yeast. The invention furthermore relates to expression constructs for expressing the yeast polypeptide in plants, preferably in plant seeds, the transgenic plants expressing the yeast polypeptide and to the use of said transgenic plants for the production of food, feeds, seed, pharmaceuticals or fine chemicals, in particular for the production of oils.
摘要翻译：本发明涉及通过从酵母中表达多肽来增加植物中油含量的方法，优选在植物种子中。本发明还涉及用于在植物中，优选在植物种子中表达酵母多肽的表达构建体，表达酵母多肽的转基因植物和所述转基因植物用于生产食品，饲料，种子，药物或精细化学品的用途，特别是用于生产油。

5. 发明申请

WO2004092367A1 USE OF GENES FOR INCREASING THE OIL CONTENT IN PLANTS 审中-公开
标题翻译：使用基因增加植物油含量
公开(公告)号：WO2004092367A1
公开(公告)日：2004-10-28
申请号：PCT/EP2004/003845
申请日：2004-04-13
申请人： BASF PLANT SCIENCE GMBH , CIRPUS, Petra , OSWALD, Oliver , RONNE, Hans , DAHLQVIST, Anders , LENMAN, Marit , NEAL, Andrea , STAHL, Ulf , LIU, Tao , BANAS, Antoni , WIBERG, Eva
发明人： CIRPUS, Petra , OSWALD, Oliver , RONNE, Hans , DAHLQVIST, Anders , LENMAN, Marit , NEAL, Andrea , STAHL, Ulf , LIU, Tao , BANAS, Antoni , WIBERG, Eva
IPC分类号： C12N15/09
CPC分类号： C12N9/1029 , C12N15/8247 , C12P7/6409 , C12P7/6463
摘要： The invention relates to methods for increasing the oil content in plants, preferably in plant seeds, by expressing the Ypr140w polypeptide from yeast or corresponding polypeptides from plants. The invention furthermore relates to expression constructs for expressing the yeast polypeptide Ypr140w or corresponding polypeptides from plants in plants, preferably in plant seeds, the transgenic plants expressing the polypeptide and to the use of said transgenic plants for the production of food, feed, seed, pharmaceuticals or fine chemicals, in particular for the production of oils.
摘要翻译：本发明涉及通过从酵母中表达Ypr140w多肽或来自植物的相应多肽来增加植物中油含量的方法，优选在植物种子中。本发明还涉及用于从植物，优选植物种子，表达多肽的转基因植物以及所述转基因植物用于生产食物，饲料，种子，植物的植物中表达酵母多肽Ypr140w或相应多肽的表达构建体。药物或精细化学品，特别是用于生产油。

6. 发明申请

WO1997037033A1 NOVEL PLANT ENZYME AND USE THEREOF 审中-公开
标题翻译：新植物酶及其使用
公开(公告)号：WO1997037033A1
公开(公告)日：1997-10-09
申请号：PCT/SE1997000247
申请日：1997-02-14
申请人： BAFOR, Maureen , BANAS, Antoni , DAHLQVIST, Anders , GUMMESON, Per-Olov , LEE, Michael , LENMAN, Marit , SJÖDAHL, Staffan , STYMNE, Sten
IPC分类号： C12P07/64
CPC分类号： C12N9/0071 , C12N15/8247 , C12P7/6427 , C12P7/6472
摘要： The present invention relates to a novel plant enzyme called delta (12) fatty acid acetylenase. This enzyme is responsible for the conversion of fatty acids to acetylenic acids and the invention relates to production of such acids. The invention also relates to use of cDNA encoding acetylenase, preferably Crepis alpina delta (12) acetylenase, for transforming organisms such as oil accumulating organisms selected from the group consisting of oil crops, oleogeneous yeasts and moulds. Furthermore, the invention relates to organisms such as oil accumulating organisms transformed with acetylenase cDNA, and to oils and other acetylenic compounds from said organisms.
摘要翻译：本发明涉及称为δ（12）脂肪酸炔酰酶的新型植物酶。该酶负责将脂肪酸转化为炔属酸，本发明涉及这种酸的生产。本发明还涉及使用编码炔酰酶（最好是Crepis alpina delta（12）acetylenase）的cDNA转化生物体，例如选自油料作物，非均质酵母和霉菌的积聚生物。此外，本发明涉及生物体，例如用炔基酶cDNA转化的油积聚生物，以及来自所述生物体的油和其它炔属化合物。

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