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1. 发明申请

WO1992008984A1 ASSAY FOR GLYCATED BLOOD PROTEINS 审中-公开
标题翻译：血糖测定蛋白质
公开(公告)号：WO1992008984A1
公开(公告)日：1992-05-29
申请号：PCT/EP1991002163
申请日：1991-11-13
申请人： COCKBAIN, Julian, Roderick, Michaelson , AXIS RESEARCH AS , SUNDREHAGEN, Erling , FRANTZEN, Frank
发明人： COCKBAIN, Julian, Roderick, Michaelson , AXIS RESEARCH AS
IPC分类号： G01N33/68
CPC分类号： G01N33/6842 , G01N33/68 , G01N33/723 , Y10S436/815 , Y10S436/824 , Y10T436/25125 , Y10T436/25375
摘要： There is provided a method of assessing a glycated blood protein in a sample, said method comprising the steps of a) optionally hemolysing said sample to liberate cell bound glycated protein; b) separating said glycated blood protein and the corresponding non-glycated blood protein from said sample; c) contacting said sample before or during separation of said glycated and non-glycated proteins therefrom or contacting said separated proteins with a first signal forming agent capable of binding to said glycated protein but not to the corresponding non-glycated protein; d) optionally, contacting said sample before or during separation of said glycated and non-glycated proteins therefrom or contacting said separated proteins with a second signal forming agent capable of binding to said glycated protein and to said corresponding non-glycated protein; and e) assessing the signal forming agents which have bound to said separated proteins and/or which have not bound to said glycated protein or said corresponding non-glycated protein; with the proviso that where said glycated protein comprises glycated hemoglobin said first signal forming agent is a chromophore labelled boronic acid or salt thereof having an absorption maximum at above 600 nm. In this method the glycated protein which is labelled by the first signal forming agent may be any one, or indeed any set, of the blood proteins. By appropriate selection of the proteins under assay an indication may be obtained of the history of the patient's blood glucose control over the short, medium and long (up to 3 months) term.
摘要翻译：提供了一种评估样品中糖化血液蛋白质的方法，所述方法包括以下步骤：a）任选地溶血所述样品以释放细胞结合的糖基化蛋白质; b）从所述样品中分离所述糖化血液蛋白质和相应的非糖化血液蛋白质; c）在分离所述糖化糖基化蛋白质和非糖化蛋白质之前或期间使所述样品接触或使所述分离的蛋白质与能够结合所述糖基化蛋白质但不与相应的非糖基化蛋白质结合的第一信号形成剂接触; d）任选地，在从所述糖基化和非糖化蛋白质分离之前或期间使所述样品接触，或使所述分离的蛋白质与能够结合所述糖基化蛋白质和所述相应的非糖基化蛋白质的第二信号形成剂接触; 和e）评估与所述分离的蛋白质结合的信号形成剂和/或不结合所述糖基化蛋白质或所述相应的非糖基化蛋白质的信号形成剂; 条件是所述糖化蛋白质包含糖化血红蛋白的所述第一信号形成剂是在600nm以上具有吸收最大值的发色团标记的硼酸或其盐。在该方法中，由第一信号形成剂标记的糖化蛋白质可以是血液蛋白质中的任何一种，或者甚至是任意组合的。通过适当选择测定中的蛋白质，可以获得关于短期，中期和长期（至多3个月）期患者血糖控制史的指示。

2. 发明申请

WO1993015220A1 HOMOCYSTEINE ASSAY 审中-公开
标题翻译：同位素测定
公开(公告)号：WO1993015220A1
公开(公告)日：1993-08-05
申请号：PCT/GB1993000138
申请日：1993-01-22
申请人： COCKBAIN, Julian, Roderick, Michaelson , AXIS RESEARCH AS , SUNDREHAGEN, Erling
发明人： COCKBAIN, Julian, Roderick, Michaelson , AXIS RESEARCH AS
IPC分类号： C12Q01/34
CPC分类号： C12Q1/48 , C07H15/14 , C07H15/26 , C07H19/16 , C12Q1/34 , G01N33/542 , G01N33/5735 , G01N33/68 , G01N33/6815
摘要： There is provided a method for assaying homocysteine in a sample, e.g. blood, plasma or urine, which comprises the steps of contacting said sample with a homocysteine converting enzyme and at least one substrate for said enzyme other than homocysteine, and without chromatographic separation assessing a non-labelled analyte selected from a homocysteine co-substrate and the homocysteine conversion products of the enzymic conversion of homocysteine by said enzyme.
摘要翻译：提供了在样品中测定同型半胱氨酸的方法，例如血液，血浆或尿液，其包括以下步骤：使所述样品与高半胱氨酸转化酶和至少一种用于除同型半胱氨酸以外的酶的底物接触，并且不进行色谱分离，评估选自同型半胱氨酸共底物的非标记分析物和所述酶对同型半胱氨酸进行酶转化的同型半胱氨酸转化产物。

3. 发明申请

WO1991019983A1 ANALYTE VARIANT ANALYSIS 审中-公开
标题翻译：分析变量分析
公开(公告)号：WO1991019983A1
公开(公告)日：1991-12-26
申请号：PCT/EP1991001145
申请日：1991-06-20
申请人： AXIS RESEARCH AS , HOLMES, Michael, John , SUNDREHAGEN, Erling
发明人： AXIS RESEARCH AS
IPC分类号： G01N33/68
CPC分类号： G01N33/6803 , G01N33/538 , G01N2333/79 , Y10S435/964 , Y10S435/975 , Y10S436/808
摘要： The present invention provides a method of assessment of the concentration of a subset of variants in a population of proteinaceous analyte variants capable of separation by a fractionation system, wherein said population of variants are contacted with a population of labelled proteinaceous specific binding partners therefor to form labelled binding partner-analyte complexes therewith, which are then subjected to separation by the said fractionation system into one or more fractions containing said subset of analyte variants in complexed form and assessment of the amount of label in one or more fractions so obtained, the said population of specific binding partners having, prior to reaction, substantially uniform distribution or mobility in said fractionation system, compositions and test kits for use in such methods. The method of the invention is particularly suitable for the analysis of variants of the protein transferrin.
摘要翻译：本发明提供了一种评估能够通过分级系统分离的蛋白质分析物变体群体中变体子集的浓度的方法，其中所述变异体群与标记的蛋白质特异性结合配偶体群体接触形成标记的结合配偶体 - 分析物复合物，然后将其通过所述分级系统分离成含有复合形式的分析物变体的所述子集的一个或多个级分，以及如此获得的一个或多个级分中标记量的评估，所述在反应之前具有特异性结合配偶体的群体在所述分级系统中具有基本均匀的分布或迁移率，用于这些方法的组合物和测试试剂盒。本发明的方法特别适用于分析蛋白转运蛋白的变体。

4. 发明申请

WO1990013818A1 GLYCOSYLATED HAEMOGLOBIN ASSAY 审中-公开
标题翻译：糖蛋白HEMOGLOBIN测定
公开(公告)号：WO1990013818A1
公开(公告)日：1990-11-15
申请号：PCT/EP1990000820
申请日：1990-05-11
申请人： HOLMES, Michael, John , AXIS RESEARCH AS , SUNDREHAGEN, Erling
发明人： HOLMES, Michael, John , AXIS RESEARCH AS
IPC分类号： G01N33/72
CPC分类号： G01N33/539 , G01N33/723 , Y10S436/815 , Y10S436/824 , Y10T436/25125 , Y10T436/25375
摘要： This invention provides a method of assessing glycosylated haemoglobin in a sample, said method comprising the steps of: (a) optionally haemolysing the sample to liberate any cell bound haemoglobin; (b) contacting the said sample or haemoglobin recovered from said sample according to step (c) below with signal-forming molecules comprising a conjugate of one or more dihydroxyboryl residues or salts thereof, linked to a signal-forming label; (c) separating glycosylated and non-glycosylated haemoglobin and any molecules bound thereto, from the sample or from the reaction mixture of step (b) above; and (d) assessing the said signal forming molecules which have bound to the separated haemoglobin, and/or any non-haemoglobin bound signal-forming molecules; and an analytical test kit for use in accordance with the method of the invention. The new assay of the invention is particularly useful for the in vitro diagnosis and monitoring of diabetes mellitus.
摘要翻译：本发明提供了一种评估样品中糖基化血红蛋白的方法，所述方法包括以下步骤：（a）任选地溶血样品以释放任何细胞结合的血红蛋白; （b）将根据下文步骤（c）从所述样品回收的所述样品或血红蛋白与包含与信号形成标记物连接的一个或多个二羟基硼烷基残基或其盐的缀合物的信号形成分子接触; （c）从样品或上述步骤（b）的反应混合物中分离糖基化和非糖基化血红蛋白和与其结合的任何分子; 和（d）评估与分离的血红蛋白结合的所述信号形成分子，和/或任何非血红蛋白结合的信号形成分子; 以及根据本发明的方法使用的分析测试试剂盒。本发明的新试验对于体外诊断和监测糖尿病特别有用。

5. 发明申请

WO1992008722A1 LABELLING AGENTS COMPRISING BORONIC ACID CONJUGATES 审中-公开
标题翻译：包含硼酸结合物的标签试剂
公开(公告)号：WO1992008722A1
公开(公告)日：1992-05-29
申请号：PCT/EP1991002160
申请日：1991-11-13
申请人： COCKBAIN, Julian, Roderick, Michaelson , AXIS RESEARCH AS , FRANTZEN, Frank , SUNDREHAGEN, Erling
发明人： COCKBAIN, Julian, Roderick, Michaelson , AXIS RESEARCH AS
IPC分类号： C07F05/02
CPC分类号： G01N33/587 , C07F5/025 , G01N33/533
摘要： Labelling agents comprising non-proteinaceous boronic acid conjugates having absorption maxima at not less than 600 nm, said label being provided by e.g. an azine, triphenylmethane, cyanine or phthalocyanine dye, are useful in the estimation and quantification of cisdiols such as glycosylated haemoglobin, by virtue of the substantially total absence of overlap with the absorption spectrum of haemoglobin. Oxazine and thiazine dyes exhibiting similar absorption characteristics and containing other activated moieties are similarly useful labelling agents, especially in the presence of haemoglobin.
摘要翻译：包含非蛋白质硼酸缀合物的标记试剂，其吸收最大值不小于600nm，所述标记由例如，吖嗪，三苯甲烷，花青或酞菁染料，由于与血红蛋白的吸收光谱基本完全不存在重叠，可用于估计和定量顺式二糖如糖基化血红蛋白。表现出相似吸收特性并含有其它活化部分的恶嗪和噻嗪染料也是类似地有用的标记试剂，特别是在血红蛋白存在下。

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