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1. 发明申请

WO2004019008A1 生体高分子の結晶化条件探査方法及び探査装置审中-公开
标题翻译：搜索生物聚合物和搜索设备的结晶条件的方法
公开(公告)号：WO2004019008A1
公开(公告)日：2004-03-04
申请号：PCT/JP2003/008765
申请日：2003-07-10
申请人：理化学研究所 , 宮武秀行 , 竹田一旗 , 三木邦夫
发明人：宮武秀行 , 竹田一旗 , 三木邦夫
IPC分类号： G01N1/28
CPC分类号： G01N1/405 , C30B7/00 , C30B29/58 , G01N21/51
摘要： Methods of searching for the crystallization conditions of a biopolymer. Namely, the crystallization conditions of a biopolymer are searched for by adding, to a solution separated from another solution containing the biopolymer by a semipermeable membrane, a substance altering the physicochemical properties of the solution, thus altering the physicochemical properties of the biopolymer-containing solution and then monitoring the state of the biopolymer-containing solution. Further, the crystallization conditions of a biopolymer are searched for by decreasing or increasing the volume of a solution containing the biopolymer separated from another solution by a semipermeable membrane to thereby altering the concentration of the biopolymer in the biopolymer-containing solution and then monitoring the state of the biopolymer-containing solution. A method of producing biopolymer crystals by using the crystallization conditions searched for the above methods. An apparatus for searching for the crystallization conditions of a biopolymer which has a dialytic crystallization chamber and a solution-mixing chamber separated therefrom by a movable semipermeable membrane. The dialytic crystallization chamber has a window for monitoring the inside of the chamber while the solution-mixing chamber is connected to one or more reagent bottles in such a manner that a solution can be supplied from a reagent bottle.
摘要翻译：搜索生物聚合物的结晶条件的方法。即，通过向通过半透膜的含有生物聚合物的另一溶液中分离的溶液中添加改变溶液的物理化学性质的物质，从而改变生物聚合物溶液的物理化学性质，来研究生物聚合物的结晶条件然后监测生物聚合物溶液的状态。此外，通过减少或增加含有通过半透膜从另一种溶液分离的生物聚合物的溶液的体积来搜索生物聚合物的结晶条件，从而改变生物聚合物溶液中生物聚合物的浓度，然后监测状态的生物聚合物溶液。通过使用搜索上述方法的结晶条件来生产生物聚合物晶体的方法。用于搜索具有透析结晶室的生物聚合物的结晶条件和通过可移动半透膜与其分离的溶液混合室的装置。透析结晶室具有用于监测室内部的窗口，同时溶液混合室以一种或多种试剂瓶的形式连接，从而可以从试剂瓶中提供溶液。

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