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1. 发明申请

WO2012054795A1 AN ULTRA SENSITIVE METHOD FOR IN SITU DETECTION OF NUCLEIC ACIDS 审中-公开
标题翻译：用于检测核酸的超敏感方法
公开(公告)号：WO2012054795A1
公开(公告)日：2012-04-26
申请号：PCT/US2011/057202
申请日：2011-10-21
申请人： ADVANCED CELL DIAGNOSTICS, INC. , WU, Xingyong , WANG, Huei-Yu Fay , SU, Nan , WANG, Li-Chong , LUO, Yuling
发明人： WU, Xingyong , WANG, Huei-Yu Fay , SU, Nan , WANG, Li-Chong , LUO, Yuling
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6841 , C12Q1/6818 , C12Q1/682 , C12Q2563/131 , C12Q2537/143 , C12Q2537/125
摘要： Disclosed is a method for in situ detection of one or more target nucleic acids based on a combination of RNAscope® method and a general ISH signal amplification method. This new method produces high signal intensity and while keeps low background noise of signal amplification. The result can be consistently reproduced and the method can be easily adopted for routine clinic diagnostic use. Further, the invention relates to a kit, comprising the components of RNAscope® assay and a general ISH signal amplification assay, for sensitive detection of one or more target nucleic acids.
摘要翻译：公开了一种基于RNA检测方法和一般ISH信号扩增方法的组合来原位检测一种或多种靶核酸的方法。这种新方法产生高信号强度，同时保持低信号放大的背景噪声。结果可以一贯重现，该方法可以方便地用于常规诊所诊断。此外，本发明涉及一种试剂盒，其包含用于灵敏检测一种或多种靶核酸的RNA检测和一般ISH信号扩增测定的组分。

2. 发明申请

WO2012040168A2 BIOMARKERS FOR DIFFERENTIATING MELANOMA FROM BENIGN NEVUS IN THE SKIN 审中-公开
标题翻译：用于从皮肤中的新陈代谢来分化MELANOMA的生物标记物
公开(公告)号：WO2012040168A2
公开(公告)日：2012-03-29
申请号：PCT/US2011/052305
申请日：2011-09-20
申请人： ADVANCED CELL DIAGNOSTICS, INC. , MA, Xiao-Jun , WU, Xingyong , LUO, Yuling
发明人： MA, Xiao-Jun , WU, Xingyong , LUO, Yuling
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q2600/112 , C12Q2600/158 , C12Q2600/16
摘要： Disclosed is a method for diagnosing melanoma in a human subject, as well as a method for providing a prognosis to a human subject who is at risk of developing melanoma recurrence, and a method for determining the stage of melanoma in a human subject, comprising the step of determining the level of expression of phosphatase and actin regulator 1 (PHACTR1) gene, or fragments thereof, either alone or in combination with the level of expression of secreted integrin-binding phosphoprotein (SPP1), preferentially expressed antigen in melanoma (PRAME), growth differentiation factor 15 (GDF15), and chemokine C-X-C motif ligand 10 (CXCL10) genes. Further, the invention relates to a diagnostic kit, comprising at least one substance for detection of the expression of PHACTR1, or fragments thereof, either alone or in combination with the detection of SPP1, PRAME, GDF15, and CXCL10, for the diagnosis or prognosis of melanoma.
摘要翻译：公开了一种用于诊断人类受试者中的黑素瘤的方法，以及用于对患有黑色素瘤复发风险的人类受试者提供预后的方法，以及用于确定人类受试者的黑色素瘤阶段的方法，包括单独或与分泌的整联蛋白结合磷蛋白（SPP1）的表达水平相结合的磷酸酶和肌动蛋白调节剂1（PHACTR1）基因或其片段的表达水平的测定步骤，优先表达黑素瘤抗原（PRAME），生长分化因子15（GDF15）和趋化因子CXC基序配体10（CXCL10）基因。此外，本发明涉及一种诊断试剂盒，其包含至少一种用于检测PHACTR1或其片段的表达的物质，或者与检测SPP1，PRAME，GDF15和CXCL10一起或与SPP1，PRAME，GDF15和CXCL10的检测结合用于诊断或预后的黑色素瘤。

3. 发明申请

WO2012103414A2 RNASCOPE® HPV ASSAY FOR DETERMINING HPV STATUS IN HEAD AND NECK CANCERS AND CERVICAL LESIONS 审中-公开
标题翻译：用于确定头颈部癌症和颈部病变中HPV状态的RNASCOPE®HPV测定
公开(公告)号：WO2012103414A2
公开(公告)日：2012-08-02
申请号：PCT/US2012/022856
申请日：2012-01-27
申请人： ADVANCED CELL DIAGNOSTICS, INC. , MA, Xiao-Jun , FLANAGAN, John , LUO, Yuling
发明人： MA, Xiao-Jun , FLANAGAN, John , LUO, Yuling
IPC分类号： C12Q1/68 , C12Q1/70
CPC分类号： C12Q1/708 , C12Q1/6841 , C12Q1/6886 , C12Q2600/112 , C12Q2600/118
摘要： The present invention provides a method and a kit for determining whether a head and neck cancer is HPV-related. In one embodiment, an RNAscope® HPV assay was designed to detect the presence of E6/E7 mRNA of certain high-risk HPV subtypes related to head and neck cancer. The present invention also provides a method and a kit for determining whether a cervical lesion is a benign lesion or a cervical intraepithethial neoplasm lesion. In one embodiment, an RNAscope® HPV assay was designed to detect the presence of E6/E7 mRNA of certain high-risk HPV subtypes related to cervical cancer. The present invention further provides a method for determining the progression of cervical intraepithethial neoplasm based on the spatial pattern and levels of the E6/E7 mRNA of certain high-risk HPV subtypes. The present invention also provides a method for determining the risk of developing cervical cancer in a human diagnosed with cervical intraepithethial neoplasm based on presence and absence of the certain subgroups of high-risk HPV subtypes.
摘要翻译：本发明提供了用于确定头颈部癌症是否与HPV相关的方法和试剂盒。在一个实施方案中，RNAscope＆reg; 设计HPV测定以检测与头颈部癌有关的某些高风险HPV亚型的E6 / E7 mRNA的存在。本发明还提供了用于确定宫颈病变是良性病变还是宫颈上皮内瘤病变的方法和试剂盒。在一个实施方案中，RNAscope＆reg; 设计HPV测定以检测与宫颈癌相关的某些高风险HPV亚型的E6 / E7 mRNA的存在。本发明进一步提供了基于某些高风险HPV亚型的E6 / E7 mRNA的空间模式和水平确定宫颈上皮内肿瘤进展的方法。本发明还提供了基于存在和不存在某些高风险HPV亚型亚组来确定诊断患有宫颈上皮内瘤的人中发展为宫颈癌的风险的方法。

4. 发明申请

WO2011091393A1 SELF-FOLDING AMPLIFICATION OF TARGET NUCLEIC ACID 审中-公开
标题翻译：目标核酸的自折叠放大
公开(公告)号：WO2011091393A1
公开(公告)日：2011-07-28
申请号：PCT/US2011/022326
申请日：2011-01-25
申请人： RD BIOSCIENCES, INC. , JU, Jingliang
发明人： JU, Jingliang
IPC分类号： C12Q1/68 , C12Q1/70
CPC分类号： C12Q1/6853 , C12N15/1096 , C12Q1/6844 , C12Q1/6865 , C12Q1/707 , C12Q2531/143 , C12Q2525/301 , C12Q2525/161 , C12Q2525/143
摘要： The application relates generally to methods useful for the selective amplification of one or more target nucleic acid or fragments thereof, as well as compositions and kits comprising said amplification reaction mixtures. More specifically, the application relates to a composite primer that comprises a 5 ' promoter portion and a 3 ' target-recognition portion which is complementary to the 3 ' end portion of a target polynucleotide sequence; and optionally, a means for identifying the 5' end portion of the target polynucleotide sequence. The amplification reaction mixture comprises at least one handle-stem-loop structure which comprises a 5 ' single-stranded handle comprising the promoter portion and a double-stranded stem comprising at least one pair of self- folding segments hybridized to each other, and optionally, a single-stranded loop comprising the sequence between the pair of self- folding segments.
摘要翻译：本申请一般涉及可用于选择性扩增一种或多种靶核酸或其片段的方法，以及包含所述扩增反应混合物的组合物和试剂盒。更具体地，本申请涉及复合引物，其包含与靶多核苷酸序列的3'端部分互补的5'启动子部分和3'靶标识别部分; 以及任选的用于鉴定靶多核苷酸序列的5'端部分的方法。扩增反应混合物包括至少一个手柄 - 茎 - 环 - 结构，其包括包含促进剂部分的5'单链手柄和包含彼此杂交的至少一对自折叠片段的双链茎，以及任选地，包括在一对折叠片段之间的序列的单链环。

5. 发明申请

WO2011038403A1 METHODS OF DETECTING NUCLEIC ACID SEQUENCES WITH HIGH SPECIFICITY 审中-公开
标题翻译：检测具有高特异性的核酸序列的方法
公开(公告)号：WO2011038403A1
公开(公告)日：2011-03-31
申请号：PCT/US2010/050569
申请日：2010-09-28
申请人： LUO, Yuling , FLANAGAN, John, James , SU, Nan , WANG, Huei-Yu, Fay
发明人： LUO, Yuling , FLANAGAN, John, James , SU, Nan , WANG, Huei-Yu, Fay
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6841 , C12Q2565/543 , C12Q2525/313
摘要： The invention relates to methods of detecting nucleic acids, including methods of detecting one or more target nucleic acid sequences in multiplex branched-chain DNA assays, are provided. Nucleic acids captured on a solid support or suspending cells are detected, for example, through cooperative hybridization events that result in specific association of a label with the nucleic acids. The invention further relates to methods to improve probe hybridization specificity and their application in genotyping. The invention also relates to in situ detection of mis-joined nucleic acid sequences. The invention relates to reducing false positive signals and improve signal-to-background ratio in hybridization-based nucleic acid detection assay. The invention further relates to method to improve specificity in hybridization based nucleic acid using co-location probes. Compositions, tissue slides, sample of suspended cells, kits, and systems related to the methods are also described.
摘要翻译：本发明涉及检测核酸的方法，包括在多重支链DNA测定中检测一种或多种靶核酸序列的方法。检测到在固体支持物或悬浮细胞上捕获的核酸，例如通过导致标记与核酸的特异性缔合的协同杂交事件。本发明还涉及改进探针杂交特异性及其在基因分型中的应用的方法。本发明还涉及错误连接的核酸序列的原位检测。本发明涉及在基于杂交的核酸检测测定中减少假阳性信号并提高信号与背景比。本发明还涉及使用共位点探针改进基于杂交的核酸的特异性的方法。还描述了组合物，组织载片，悬浮细胞样品，试剂盒和与该方法相关的系统。

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