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1. 发明申请

WO2020097607A1 MASSIVELY PARALLEL SEQUENCING USING UNLABELED NUCLEOTIDES 审中-公开
公开(公告)号：WO2020097607A1
公开(公告)日：2020-05-14
申请号：PCT/US2019/060779
申请日：2019-11-11
申请人： MGI TECH CO., LTD. , BGI SHENZEN CO., LTD.
发明人： DRMANAC, Snezana , LI, Handong , CALLOW, Matthew, J. , ECKHARDT, Leon , GABLENZ, Scott , DRMANAC, Radoje , ZHOU, Ping
IPC分类号： C12Q1/6874 , C12Q1/6876
摘要： The invention provides compositions and methods for sequencing nucleic acids and other applications. In sequencing by synthesis, unlabeled reversible terminators are incorporated by a polymerase in each cycle, then labeled after incorporation by binding to the reversible terminator a directly or indirectly labeled antibody or other affinity reagent.

2. 发明申请

WO2020033438A1 NUCLEIC ACID SEQUENCE ENRICHMENT BY DEFINED NUCLEIC ACID-DIRECTED ENDONUCLEASE DIGESTION 审中-公开
公开(公告)号：WO2020033438A1
公开(公告)日：2020-02-13
申请号：PCT/US2019/045352
申请日：2019-08-06
申请人： CHAN ZUCKERBERG BIOHUB, INC.
发明人： CRAWFORD, Emily, D. , QUAN, Jenai
IPC分类号： C12N9/22 , C12N15/10 , C12Q1/68
摘要： A method of sample analysis is described comprising providing a sample comprising a plurality of endblocked polynucleotides, digesting the sample with one or more defined nucleic acid-directed endonuclease that targets a sequence of interest to produce a digested sample of polynucleotide fragments, wherein one or more of the fragments in the digested sample comprises: a sequence of interest and at least one ligatable end that has been generated by endonuclease cleavage; (c) enriching for fragments that contain the sequence of interest, wherein the one or more sequences of interest are enriched greater than 55 times, or greater than 750 times, their relative abundance in the sample; and (d) analyzing the enriched sample.

3. 发明申请

WO2014015278A1 PRODUCTION OF FATTY ALCOHOLS FROM ENGINEERED MICROORGANISMS 审中-公开
标题翻译：从工程微生物生产脂肪醇
公开(公告)号：WO2014015278A1
公开(公告)日：2014-01-23
申请号：PCT/US2013/051340
申请日：2013-07-19
申请人： CODEXIS, INC.
发明人： VROOM, Jonathan , BALATSKAYA, Svetlana , BARAK, Yoram , CLARK, Louis , CHOUDHARY, Trish , KARLSHOEJ, Kristian , VALLE, Fernando , CHAN, Kaman
IPC分类号： C07C29/00 , C07C31/00 , C07C31/02 , C07C33/00 , C07C33/02 , C07C43/00
CPC分类号： C12P7/64 , A61K8/342 , A61K2800/85 , C11D3/2013 , C12N9/0006 , C12N9/0008 , C12N9/001 , C12N9/1007 , C12N9/1029 , C12N9/16 , C12N9/88 , C12N9/93 , C12P7/04 , C12Y101/01 , C12Y101/011 , C12Y103/01009 , C12Y103/99 , C12Y201/01005 , C12Y203/01029 , C12Y203/01041 , C12Y203/01086 , C12Y203/0118 , C12Y301/02 , C12Y301/02001 , C12Y301/02027 , C12Y402/01059 , C12Y602/01 , C12Y602/01003
摘要： Recombinant microorganisms are provided which have been engineered to produce fatty alcohols. Also provided are recombinant microorganisms which comprise a heterologous polynucleotide encoding a fatty alcohol reductase enzyme and an introduced polynucleotide encoding a β-ketoacyl acyl carrier protein synthase.
摘要翻译：提供了重组微生物，其已被工程化以生产脂肪醇。还提供了包含编码脂肪醇还原酶的异源多核苷酸和编码β-酮酰酰基载体蛋白合酶的引入的多核苷酸的重组微生物。

4. 发明申请

WO2012154876A1 PROBE BASED NUCLEIC ACID DETECTION 审中-公开
标题翻译：基于探针的核酸检测
公开(公告)号：WO2012154876A1
公开(公告)日：2012-11-15
申请号：PCT/US2012/037155
申请日：2012-05-09
申请人： FLUIDIGM CORPORATION , LIVAK, Kenneth J. , MYERS, Stacey , WANG, Xiaohui , WANG, Jun
发明人： LIVAK, Kenneth J. , MYERS, Stacey , WANG, Xiaohui , WANG, Jun
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6818 , C12Q2525/151
摘要： The invention provides a method for detecting a target nucleotide sequence by tagging the nucleotide sequence with a nucleotide tag, providing a probe oligonucleotide with a melting temperature Tm1, comprising a regulatory sequence and a nucleotide tag recognition sequence; incorporating the probe oligonucleotide into the tagged polynucleotide in a polynucleotide amplification reaction, providing a regulatory oligonucleotide with a melting temperature Tm2, comprising a sequence segment that is at least partially complementary to the regulatory sequence, amplifying the tagged target nucleic acid sequence in a PCR amplification reaction using the probe oligonucleotide as a primer, and detecting the amplification product; wherein Tm1 and Tm2 are higher than the annealing temperature associated with the polynucleotide amplification reaction.
摘要翻译：本发明提供了通过用核苷酸标签标记核苷酸序列来检测靶核苷酸序列的方法，提供了具有调节序列和核苷酸标签识别序列的融解温度Tm1的探针寡核苷酸; 在多核苷酸扩增反应中将探针寡核苷酸掺入标记的多核苷酸中，提供具有解链温度Tm2的调节性寡核苷酸，其包含与调节序列至少部分互补的序列片段，在PCR扩增中扩增标记的靶核酸序列使用探针寡核苷酸作为引物的反应，检测扩增产物; 其中Tm1和Tm2高于与多核苷酸扩增反应相关的退火温度。

5. 发明申请

WO2012142611A2 SEQUENCING SMALL AMOUNTS OF COMPLEX NUCLEIC ACIDS 审中-公开
标题翻译：序列复合核酸的小数量
公开(公告)号：WO2012142611A2
公开(公告)日：2012-10-18
申请号：PCT/US2012/033832
申请日：2012-04-16
申请人： COMPLETE GENOMICS, INC. , DRMANAC, Radoje , PETERS, Brock A. , KERMANI, Bahram Ghaffarzadeh
发明人： DRMANAC, Radoje , PETERS, Brock A. , KERMANI, Bahram Ghaffarzadeh
CPC分类号： G06F19/22 , C12Q1/6869 , C12Q2537/159 , C12Q2549/10
摘要： The present invention provides methods and compositions for sequencing small amounts of complex nucleic acids such as human genomes and for analyzing the resulting sequence information in order to reduce sequencing errors and perform haplotype phasing, for example.
摘要翻译：本发明提供了用于测序少量复合核酸如人类基因组的方法和组合物，并且用于分析所得序列信息以减少测序误差并进行例如单倍型定相。

6. 发明申请

WO2009103010A2 ALPHA-SYNUCLEIN KINASE 审中-公开
标题翻译： ALPHA-SYNUCLEIN激酶
公开(公告)号：WO2009103010A2
公开(公告)日：2009-08-20
申请号：PCT/US2009/034135
申请日：2009-02-13
申请人： ELAN PHARMACEUTICALS, INC. , ANDERSON, John P. , BANDUCCI, Kelly , BASI, Guriqbal S. , CHEREAU, David , CHILCOTE, Tamie J. , FRIGNON, Normand, L., Jr. , GOLDSTEIN, Jason , GRISWOLD-PRENNER, Irene
发明人： ANDERSON, John P. , BANDUCCI, Kelly , BASI, Guriqbal S. , CHEREAU, David , CHILCOTE, Tamie J. , FRIGNON, Normand, L., Jr. , GOLDSTEIN, Jason , GRISWOLD-PRENNER, Irene
IPC分类号： G01N33/50
CPC分类号： C12Q1/485 , C12N15/1137 , C12N2310/14 , G01N2500/04 , G01N2800/2835
摘要： Agents and methods for treatment of diseases associated with Lewy body diseases (LBDs) in the brain of a patient are provided. Preferred agents include inhibitors of PLK2 kinase.
摘要翻译：提供了用于治疗与患者脑中路易体疾病（LBD）相关的疾病的药剂和方法。优选的试剂包括PLK2激酶的抑制剂。

7. 发明申请

WO2009073560A1 INDUSTRIAL PRODUCTION OF ORGANIC COMPOUNDS USING RECOMBINANT ORGANISMS EXPRESSING METHYL HALIDE TRANSFERASE 审中-公开
标题翻译：使用表达甲基卤化物转移酶的重组体进行工业化生产有机化合物
公开(公告)号：WO2009073560A1
公开(公告)日：2009-06-11
申请号：PCT/US2008/085019
申请日：2008-11-26
申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA , SANTI, Daniel, V. , VOIGT, Christopher, A. , SANTI, Daniel, V. , BAYER, Travis, S.
发明人： VOIGT, Christopher, A. , SANTI, Daniel, V. , BAYER, Travis, S.
IPC分类号： C12N1/00 , C12Q1/48
CPC分类号： C12N9/10 , C12P5/00 , Y02E50/16
摘要： The invention relates to a process for production of organic compounds using genetically engineered organisms expressing a S-adenosylmethionine (SAM)- dependent methyl halide transferase and, optionally modified at loci that affect flux through SAM metabolic pathways or affect intracellular halide levels. In one approach the organism, halides (chlorine, bromine and/or iodine); and a carbon source are incubated in a cultivation medium under conditions in which methyl halide is produced. The methyl halide may be collected and converted into non- halogenated organic molecules.
摘要翻译：本发明涉及使用表达S-腺苷甲硫氨酸（SAM）依赖性甲基卤转移酶的遗传工程生物制备有机化合物的方法，并且任选地在通过SAM代谢途径影响通量或影响细胞内卤化物水平的位点上修饰。在一种方法中，生物体，卤化物（氯，溴和/或碘）; 并将碳源在培养基中在产生甲基卤的条件下培养。甲基卤化物可以被收集并转化成非卤化的有机分子。

8. 发明申请

WO2009073557A2 BIOLOGICAL SYSTEMS FOR PRODUCTION OF COMMERCIALLY VALUABLE COMPOUNDS 审中-公开
标题翻译：用于生产商业有价值化合物的生物系统
公开(公告)号：WO2009073557A2
公开(公告)日：2009-06-11
申请号：PCT/US2008/085013
申请日：2008-11-26
申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA , VOIGT, Christopher, A. , BAYER, Travis, S.
发明人： VOIGT, Christopher, A. , BAYER, Travis, S.
IPC分类号： C12P7/10
CPC分类号： C12N9/10 , C12P5/00 , Y02E50/16
摘要： The invention relates to systems and methods for production of compounds by yeast and other organisms. In one approach yeast engineered for production of a compound of commercial value is cultured together with a cellulosic bacteria, and the yeast uses a metabolic product produced by the bacteria as a carbon source. Methyl halides are an example of compounds that may be produced by this process. The invention also relates to production of organic compounds using genetically engineered organisms expressing a S-adenosylmethionine (SAM)-dependent methyl halide transferase. In one approach the organism, halides and a carbon source are incubated in a cultivation medium under conditions in which methyl halide is produced. The methyl halide may be collected and converted into non-halogenated organic molecules.
摘要翻译：本发明涉及由酵母和其他生物体生产化合物的系统和方法。在一种方法中，用于生产商业价值化合物的酵母菌与纤维素细菌一起培养，酵母使用由细菌产生的代谢产物作为碳源。甲基卤化物是可以通过该方法生产的化合物的实例。本发明还涉及使用表达S-腺苷甲硫氨酸（SAM） - 依赖性甲基卤转移酶的遗传工程生物体生产有机化合物。在一种方法中，生物体，卤化物和碳源在培养基中在产生甲基卤的条件下孵育。甲基卤可以被收集并转化成非卤化的有机分子。

9. 发明申请

WO2009048883A1 DIAGNOSIS OF AGE-RELATED MACULAR DEGENERATION USING BIOMARKERS 审中-公开
标题翻译：使用生物标志物诊断与年龄相关的肉瘤变性
公开(公告)号：WO2009048883A1
公开(公告)日：2009-04-16
申请号：PCT/US2008/079104
申请日：2008-10-07
申请人： UNIVERSITY OF IOWA RESEARCH FOUNDATION , HAGEMAN, Gregory S.
发明人： HAGEMAN, Gregory S.
IPC分类号： G01N33/53 , G01N24/00
CPC分类号： G01N33/6893 , G01N33/6851 , G01N2800/164 , G01N2800/52
摘要： The invention relates to proteins associated with age-related macular degeneration (AMD). These proteins, which are present in blood, are expressed in individuals with AMD at either elevated or reduced levels compared to healthy individuals. The invention provides methods for diagnosing AMD. The invention provides methods for assessing the efficacy of treatment of AMD. The invention provides methods for monitoring the progression of AMD.
摘要翻译：本发明涉及与年龄相关性黄斑变性（AMD）相关的蛋白质。存在于血液中的这些蛋白质在与健康个体相比处于升高或降低的水平的个体中表达。本发明提供了诊断AMD的方法。本发明提供了评估AMD治疗功效的方法。本发明提供了监测AMD进展的方法。

10. 发明申请

WO2008089493A2 HIGH EFFICIENCY AND HIGH PRECISION MICROFLUIDIC DEVICES AND METHODS 审中-公开
标题翻译：高效率和高精度微流体装置和方法
公开(公告)号：WO2008089493A2
公开(公告)日：2008-07-24
申请号：PCT/US2008/051728
申请日：2008-01-22
申请人： FLUIDIGM CORPORATION , COHEN, David, S. , WANG, Jing , MAY, Andrew, P. , JONES, Robert, C. , NASSEF, Hany
发明人： COHEN, David, S. , WANG, Jing , MAY, Andrew, P. , JONES, Robert, C. , NASSEF, Hany
IPC分类号： B01L3/00
CPC分类号： B01L3/502738 , B01L3/5025 , B01L7/52 , B01L2200/0684 , B01L2300/0816 , B01L2300/0864 , B01L2300/087 , B01L2300/0874 , B01L2300/0887 , B01L2300/123 , B01L2400/0487 , B01L2400/0605 , B01L2400/0638 , B01L2400/0655 , B33Y80/00 , F16K99/0001 , F16K99/0026 , F16K99/0059 , F16K2099/0074 , F16K2099/0078 , F16K2099/008 , F16K2099/0084 , Y10T137/0318 , Y10T137/85938
摘要： New high density microfluidic devices and methods provide precise metering of fluid volumes and efficient mixing of the metered volumes. A first solution is introduced into a segment of a flow channel in fluidic communication with a reaction chamber. A second solution is flowed through the segment so that the first solution is displaced into the reaction chamber, and a volume of the second solution enters the chamber. The chamber can then be isolated and reactions within the chamber can be initiated and/or detected. High throughput methods of genetic analysis can be carried out with greater accuracy than previously available.
摘要翻译：新的高密度微流体装置和方法提供流体体积的精确计量和计量体积的有效混合。将第一溶液引入与反应室流体连通的流动通道的段中。第二溶液流过该段，使得第一溶液移入反应室，并且第二溶液的体积进入该室。然后可以分离室，并且可以启动和/或检测室内的反应。遗传分析的高通量方法可以比以前更高的精度进行。

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