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1. 发明申请

WO2008092102A9 PRESSURE CYCLING SYSTEMS AND RELATED METHOD 审中-公开
标题翻译：压力循环系统及相关方法
公开(公告)号：WO2008092102A9
公开(公告)日：2010-11-18
申请号：PCT/US2008052089
申请日：2008-01-25
申请人： PRESSURE BIOSCIENCES INC , TING EDMUND Y
发明人： TING EDMUND Y
IPC分类号： B01J3/00 , B01J3/03 , C12M1/00
CPC分类号： B01J3/00 , B01J3/03 , B01J2219/00065 , B01J2219/00162 , B01J2219/002 , B01J2219/00213 , B01J2219/00231
摘要： A pressure cycling system includes a reaction chamber configured to receive a sample and a charge pump in fluid communication with the reaction chamber. The charge pump is operable to convey a fluid from a fluid source toward the reaction chamber. The system also includes a check valve disposed between the charge pump and the reaction chamber. The check valve is operable to inhibit the flow of fluid from the reaction chamber toward the charge pump. A pressure intensifier is in fluid communication with the reaction chamber. The pressure intensifier is pneumatically operable to adjust a pressure in the reaction chamber. A controller is configured to control operation of the charge pump and the pressure intensifier. The controller is configured to pressurize the reaction chamber to a first pressure through operation of the charge pump. The controller is also configured to fluctuate the pressure in the reaction chamber between a second pressure and a third pressure through operation of the pressure intensifier.
摘要翻译：压力循环系统包括配置成接收样品的反应室和与反应室流体连通的电荷泵。电荷泵可操作以将流体从流体源传送到反应室。该系统还包括设置在电荷泵和反应室之间的止回阀。止回阀可操作以阻止流体从反应室朝向电荷泵的流动。增压器与反应室流体连通。增压器可气动地操作以调节反应室中的压力。控制器被配置为控制电荷泵和增压器的操作。控制器被配置为通过操作电荷泵将反应室加压至第一压力。控制器还被配置为通过操作压力增加器来在第二压力和第三压力之间波动反应室中的压力。

2. 发明申请

WO2010033740A2 SHREDDER FOR MECHANICAL DISRUPTION BY GENTLE CONTROLLED COMPRESSIVE ROTATION 审中-公开
标题翻译：用于机械控制压缩旋转的机械式破坏机
公开(公告)号：WO2010033740A2
公开(公告)日：2010-03-25
申请号：PCT/US2009/057370
申请日：2009-09-17
申请人： PRESSURE BIOSCIENCES INC. , TING, Edmund, Y. , LAZAREV, Alexander , GROSS, Vera , DUSSAULT, Charles , LI, Chunqin , LAWRENCE, Nathan , SCHUMACHER, Richard, T.
发明人： TING, Edmund, Y. , LAZAREV, Alexander , GROSS, Vera , DUSSAULT, Charles , LI, Chunqin , LAWRENCE, Nathan , SCHUMACHER, Richard, T.
IPC分类号： G01N1/28
CPC分类号： G01N1/286 , C12M35/04 , G01N2001/2866
摘要： The systems and techniques of the present invention can also synergistically utilize mechanical disruption processes with the use of high hydrostatic pressure extraction, such as pressure cycling extraction techniques to achieve high yield of difficult to extract sample constituents without generating high shear stress or high temperatures.
摘要翻译：本发明的系统和技术还可以协同地利用机械破碎过程，使用高静态压力提取，例如压力循环提取技术，以实现高产率的难以提取样品组分而不产生高剪切应力或高温。

3. 发明申请

WO2009137695A9 EXTRACTION OF BIOMOLECULAR COMPLEXES ASSISTED BY ALTERNATING HYDROSTATIC PRESSURE 审中-公开
公开(公告)号：WO2009137695A9
公开(公告)日：2009-11-12
申请号：PCT/US2009/043168
申请日：2009-05-07
申请人： PRESSURE BIOSCIENCES INC. , TAO, Feng , SCHUMACHER, Richard , LAWRENCE, Nathan , GROSS, Vera , SMEJKAL, Gary , LAZAREV, Alexander , HAN, Rui
发明人： TAO, Feng , SCHUMACHER, Richard , LAWRENCE, Nathan , GROSS, Vera , SMEJKAL, Gary , LAZAREV, Alexander , HAN, Rui
IPC分类号： C12M1/33 , G01N30/28
摘要： Extraction methods that allow a molecular complex (e.g., an organelle) to be extracted from a sample by employing pressure cycling are described.

4. 发明申请

WO2017044590A1 ULTRAHIGH PRESSURE COMPACT VALVE WITH THROTTLING CAPABILITY 审中-公开
标题翻译：超高压紧凑型阀具有屈曲能力
公开(公告)号：WO2017044590A1
公开(公告)日：2017-03-16
申请号：PCT/US2016/050717
申请日：2016-09-08
申请人： PRESSURE BIOSCIENCES, INC.
发明人： TING, Edmund, Y. , LAZAREV, Alexander , MA, Jun
IPC分类号： F16K3/24 , F16K1/12 , F16K1/38 , F16K31/62
CPC分类号： F16K1/12 , F16K1/14 , F16K1/38 , F16K3/24 , F16K31/62
摘要： A high pressure valve includes a lever and a variable force generator for facilitating control of opening and closing the valve at high pressures. The high pressure valve includes a housing having a chamber providing fluid communication between a first port and a second port, a pin movable within the chamber between an open position and a closed position. A first end of a lever is coupled to the pin and a second end of the lever is coupled to a variable force generator. The lever pivots about a pivot point. A controller coupled to the variable force generator is configured to adjust a force applied to the second end of the lever by the variable force generator to control the movement of the pin between the open position and the closed position.
摘要翻译：高压阀包括杠杆和可变力发生器，用于在高压下便于控制阀的打开和关闭。高压阀包括壳体，壳体具有在第一端口和第二端口之间提供流体连通的腔室，在腔室内在打开位置和关闭位置之间可移动的销。杠杆的第一端联接到销，并且杆的第二端联接到可变力发生器。杠杆围绕枢轴点枢转。耦合到可变力发生器的控制器被配置为通过可变力发生器来调节施加到杆的第二端的力，以控制销在打开位置和关闭位置之间的运动。

5. 发明申请

WO2013052696A1 HIGH PRESSURE SAMPLE CONTAINMENT SYSTEM FOR ELECTROMAGNETIC MEASUREMENTS 审中-公开
标题翻译：用于电磁测量的高压样品容纳系统
公开(公告)号：WO2013052696A1
公开(公告)日：2013-04-11
申请号：PCT/US2012/058792
申请日：2012-10-04
申请人： PRESSURE BIOSCIENCES, INC.
发明人： TING, Edmund, Y. , LAZAREV, Alexander
IPC分类号： G01V3/00
CPC分类号： G01R33/305 , G01N24/10 , G01R33/60
摘要： The present invention is related to systems and methods for chemical and biological analysis and, in particular, to systems, apparatus, and methods of sample conditioning and analysis.
摘要翻译：本发明涉及用于化学和生物分析的系统和方法，特别涉及样品调节和分析的系统，装置和方法。

6. 发明申请

WO2009137695A3 EXTRACTION OF BIOMOLECULAR COMPLEXES ASSISTED BY ALTERNATING HYDROSTATIC PRESSURE 审中-公开
标题翻译：通过替代静水压力来提取生物分子复合物
公开(公告)号：WO2009137695A3
公开(公告)日：2009-12-23
申请号：PCT/US2009043168
申请日：2009-05-07
申请人： PRESSURE BIOSCIENCES INC , TAO FENG , SCHUMACHER RICHARD , LAWRENCE NATHAN , GROSS VERA , SMEJKAL GARY , LAZAREV ALEXANDER , HAN RUI
发明人： TAO FENG , SCHUMACHER RICHARD , LAWRENCE NATHAN , GROSS VERA , SMEJKAL GARY , LAZAREV ALEXANDER , HAN RUI
IPC分类号： C12M1/33 , G01N30/28
CPC分类号： C12N1/066 , C12N13/00
摘要： Extraction methods that allow a molecular complex (e.g., an organelle) to be extracted from a sample by employing pressure cycling are described.
摘要翻译：描述了允许通过使用压力循环从样品中提取分子复合物（例如细胞器）的提取方法。

7. 发明申请

WO2012024523A3 FLOW-THROUGH HIGH HYDROSTATIC PRESSURE MICROFLUIDIC SAMPLE PREPARATION DEVICE AND RELATED METHODS THEREFOR 审中-公开
公开(公告)号：WO2012024523A3
公开(公告)日：2012-02-23
申请号：PCT/US2011/048302
申请日：2011-08-18
申请人： PRESSURE BIOSCIENCES, INC. , LAZAREV, Alexander
发明人： PRESSURE BIOSCIENCES, INC. , LAZAREV, Alexander
IPC分类号： G01N1/10
摘要： Described herein is a sample preparation device including a sample delivery source, an inline means of transferring the sample from the sample source into a deformable channel within a pressure vessel, and out of the channel into downstream analysis components, a deformable channel disposed within the pressure vessel, the deformable channel having an inlet end and an outlet end fluidly connectable to high pressure valves and a means to measure the fluid pressure within the deformable channel, an external source of a controlled pressurized fluid fluidly connectable to the pressure vessel and a controller system that monitors and controls the sample fluid pressure by control of the external pressure vessel fluid.

8. 发明申请

WO2010129704A3 MICROTUBE AND RELATED METHODS THEREFOR 审中-公开
标题翻译： MICROTUBE及其相关方法
公开(公告)号：WO2010129704A3
公开(公告)日：2011-02-03
申请号：PCT/US2010033770
申请日：2010-05-05
申请人： PRESSURE BIOSCIENCES INC , TING EDMUND , LAZAREV ALEXANDER , SCHUMACHER RICHARD , DUSSAULT CHARLES , FRITZSCHE WAYNE
发明人： TING EDMUND , LAZAREV ALEXANDER , SCHUMACHER RICHARD , DUSSAULT CHARLES , FRITZSCHE WAYNE
IPC分类号： G01N1/28
CPC分类号： G01N1/286 , B01L3/505 , B01L3/5082 , B01L9/06 , B01L2300/14
摘要： Sample processing devices having collapsible, flexible bodies are disclosed. The flexible microtube sampling devices are utilizable in pressure mediated, pressure cycling procedures. Externally applied pressure on the flexible microtube sample processing devices allow buckling thereof and transfer of the applied pressure to the sample contained therein.
摘要翻译：公开了具有可折叠，柔性体的样品处理装置。柔性微管采样装置可用于压力介导的压力循环程序。柔性微管样品处理装置上的外部施加的压力允许其弯曲并将施加的压力转移到其中所含的样品。

9. 发明申请

WO2010129704A2 MICROTUBE AND RELATED METHODS THEREFOR 审中-公开
标题翻译： MICROTUBE及其相关方法
公开(公告)号：WO2010129704A2
公开(公告)日：2010-11-11
申请号：PCT/US2010/033770
申请日：2010-05-05
申请人： PRESSURE BIOSCIENCES INC. , TING, Edmund , LAZAREV, Alexander , SCHUMACHER, Richard , DUSSAULT, Charles , FRITZSCHE, Wayne
发明人： TING, Edmund , LAZAREV, Alexander , SCHUMACHER, Richard , DUSSAULT, Charles , FRITZSCHE, Wayne
IPC分类号： G01N1/28
CPC分类号： G01N1/286 , B01L3/505 , B01L3/5082 , B01L9/06 , B01L2300/14
摘要： Sample processing devices having collapsible, flexible bodies are disclosed. The flexible microtube sampling devices are utilizable in pressure mediated, pressure cycling procedures. Externally applied pressure on the flexible microtube sample processing devices allow buckling thereof and transfer of the applied pressure to the sample contained therein.
摘要翻译：公开了具有可折叠，柔性体的样品处理装置。柔性微管采样装置可用于压力介导的压力循环程序。柔性微管样品处理装置上的外部施加的压力允许其弯曲并将施加的压力转移到其中所含的样品。

10. 发明申请

WO2010033740A3 SHREDDER FOR MECHANICAL DISRUPTION BY GENTLE CONTROLLED COMPRESSIVE ROTATION 审中-公开
标题翻译：由温德勒控制的压缩旋转引起的机械破坏的SHREDDER
公开(公告)号：WO2010033740A3
公开(公告)日：2010-05-06
申请号：PCT/US2009057370
申请日：2009-09-17
申请人： PRESSURE BIOSCIENCES INC , TING EDMUND Y , LAZAREV ALEXANDER , GROSS VERA , DUSSAULT CHARLES , LI CHUNQIN , LAWRENCE NATHAN , SCHUMACHER RICHARD T
发明人： TING EDMUND Y , LAZAREV ALEXANDER , GROSS VERA , DUSSAULT CHARLES , LI CHUNQIN , LAWRENCE NATHAN , SCHUMACHER RICHARD T
IPC分类号： G01N1/28
CPC分类号： G01N1/286 , C12M35/04 , G01N2001/2866
摘要： The systems and techniques of the present invention can also synergistically utilize mechanical disruption processes with the use of high hydrostatic pressure extraction, such as pressure cycling extraction techniques to achieve high yield of difficult to extract sample constituents without generating high shear stress or high temperatures.
摘要翻译：本发明的系统和技术还可协同利用机械破碎过程，使用高流体静力学压力提取（例如压力循环提取技术）以实现难以提取样品成分的高产率而不产生高剪切应力或高温。

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