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71. 发明申请

WO01083702A2 NOVEL MEMBERS OF THE LYSYL OXIDASES FAMILY OF AMINE OXIDASES RELATED APPLICATIONS 审中-公开
标题翻译：胺氧化物的氧化亚铁的新成员相关申请
公开(公告)号：WO01083702A2
公开(公告)日：2001-11-08
申请号：PCT/US2001/014472
申请日：2001-05-03
IPC分类号： C12N9/06 , C12N
CPC分类号： C12N9/0022 , A01K2217/075 , C12N9/0024
摘要： In accordance with the present invention, there are provided additional LOX-like proteins characterized by their structural homology to LOX, LOXL1 and LOXL2, and containing a copper binding domain and a catalytic domain. Nucleic acid sequences encoding LOXL3 and LOXL4 proteins, antibodies derived threfrom, as well as assays employing these proteins for determining tissue distribution and catalytic activites, are also disclosed. Invention proteins can be employed in a variety of ways, such as, for example, in bioassays, for production of antibodies thereto, in therapeutic compositions containing such proteins and/or antibodies. The present invention also discloses methods and diagnostic systems for determining the levels or activities of proteins (or functional fragments thereof) related to the LOX gene family, methods for producing a transgenic animal model comprising disrupted genes encoding proteins (or fragments thereof) related to LOX gene family, and methods for identifying compound(s) which modulate the activity of proteins (or functional fragments thereof) related to the LOX gene family. The present invention therefore, discloses the gene structure, chromosomal localization, protein domain structure, evolutionary relationship, tissue specific expression and distribution of lysyl oxidase (LOX) and LOX-like proteins including but not limited to LOXL, LOXL2, LOXL3 and LOXL4, that defines a family of proteins present in distinct cellular and tissue locations, each with a related but different function.
摘要翻译：根据本发明，提供了另外的LOX样蛋白，其特征在于它们与LOX，LOXL1和LOXL2的结构同源性，并且含有铜结合结构域和催化结构域。还公开了编码LOXL3和LOXL4蛋白的核酸序列，衍生的抗体的抗体，以及使用这些蛋白质测定组织分布和催化活性的测定法。本发明的蛋白质可以以各种方式使用，例如在生物测定中用于在其中产生抗体，在含有这些蛋白质和/或抗体的治疗组合物中使用。本发明还公开了用于确定与LOX基因家族相关的蛋白质（或其功能片段）的水平或活性的方法和诊断系统，用于产生转录动物模型的方法包括编码与LOX相关的蛋白质（或其片段）的破坏基因基因家族，以及用于鉴定调节与LOX基因家族相关的蛋白质（或其功能片段）的活性的化合物的方法。因此，本发明公开了包括但不限于LOXL，LOXL2，LOXL3和LOXL4的赖氨酰氧化酶（LOX）和LOX样蛋白质的基因结构，染色体定位，蛋白质结构域，进化关系，组织特异性表达和分布，定义了存在于不同细胞和组织位置的蛋白质家族，每个具有相关但不同的功能。

72. 发明申请

WO01059078A2 AGGREGATE-FREE URATE OXIDASE FOR PREPARATION OF NON-IMMUNOGENIC POLYMER CONJUGATES 审中-公开
标题翻译：无免疫尿素氧化酶用于制备非免疫聚合物结合物
公开(公告)号：WO01059078A2
公开(公告)日：2001-08-16
申请号：PCT/US2001/040069
申请日：2001-02-07
IPC分类号： A61K38/00 , A61K38/44 , A61K47/48 , A61P19/06 , C12N9/06 , C12N9/96
CPC分类号： A61K47/48215 , A61K38/00 , A61K47/60 , C12N9/0046 , C12N9/96
摘要： A naturally occurring or recombinant protein, especially a mutein of porcine urate oxidase (uricase), that is essentially free of large aggregates can be rendered substantially non-immunogenic by conjugation with a sufficiently small number of strands of polymer such that the bioactivity of the protein is essentially retained in the conjugate. Such conjugates are unusually well suited for treatment of chronic conditions because they are less likely to induce the formation of antibodies and/or accelerated clearance than are similar conjugates prepared from protein preparations containing traces of large aggregates.
摘要翻译：基本上不含大量聚集体的天然存在或重组的蛋白质，特别是猪尿酸氧化酶（尿酸酶）的突变蛋白质可通过与足够少数量的聚合物链共轭而使其基本上不具有免疫原性，使得蛋白质的生物活性基本上保留在缀合物中。这种缀合物非常适合用于治疗慢性病症，因为它们与由含有痕量的大聚集体的蛋白质制剂制备的类似共轭物相比不太可能诱导抗体形成和/或加速清除。

73. 发明申请

WO01053323A2 PLANTS TOLERANT OF ENVIRONMENTAL STRESS CONDITIONS, METHODS OF GENERATING SAME AND NOVEL POLYNUCLEOTIDE SEQUENCE UTILIZED THEREBY 审中-公开
标题翻译：植物严酷的环境压力，这种植物的制备方法以及所用的多核苷酸序列
公开(公告)号：WO01053323A2
公开(公告)日：2001-07-26
申请号：PCT/IL2001/000026
申请日：2001-01-10
IPC分类号： C12N9/06 , C12N15/82 , C07K
CPC分类号： C12N9/0026 , C12N15/8237 , C12N15/8251 , C12N15/8273
摘要： A method of generating a plant tolerant of environmental stress conditions, the method comprising the step of expressing within the plant exogenous polynucleotides encoding enzymes capable of catalyzing proline production, wherein the exogenous polynucleotides are expressed in a manner so as to allow accumulation of the enzymes within a subcellular organelle of the plant. The invention further describes novel polynucleotides and nucleic acid constructs useful in implementing the methods and a transgenic plant resultant therefrom.
摘要翻译：本发明涉及生产耐受环境胁迫的植物的方法，其包括在植物中表达编码能够催化脯氨酸生产的外源多核苷酸的酶。表达多核苷酸以便它们可以将酶积累在植物的亚细胞器中。本发明还涉及用于实施所述方法的多核苷酸和核酸产物，以及所得到的转基因植物。

74. 发明申请

WO01051622A2 P450 MONOOXYGENASES OF THE CYP79 FAMILY 审中-公开
标题翻译： CYP79家族的P450单克隆抗体
公开(公告)号：WO01051622A2
公开(公告)日：2001-07-19
申请号：PCT/EP2001/000297
申请日：2001-01-11
IPC分类号： A01H5/00 , C12N9/02 , C12N9/06 , C12N15/09 , C12N15/82
CPC分类号： C12N9/0071 , C12N15/8243 , C12N15/8251 , C12N15/8253 , C12N15/8254
摘要： The invention provides DNA coding for cytochrome P450 monooxygenases of the CYP79 family catalyzing the conversion of an aliphatic or aromatic acid or chain-elongated methionine homologue to the corresponding oxime. Preferred embodiments of the invention are enzymes catalyzing the conversion of L-Valine and L-Isoleucine such as the cassava enzymes CYP79D1 and CYP79D2, enzymes catalyzing the conversion of tyrosine such as the Triglochin maritima enzymes CYP79E1 and CYP79E2, enzymes catalyzing the conversion of tryptophan to the corresponding oxime indole-3-acetaldoxine such as the Arabidopsis thaliana enzyme CYP79A2 and the Bassica napus enzyme CYP79B5, and enzymes catalyzing the conversion of a chain-elongated methionine homologue such as the Arabidopsis thaliana enzymes CYP79F1 and CYP79F2. Transgenic expression of said DNA or parts thereof in plants can be used to manipulate the biosynthesis of corresponding glucosinolates or cyanogenic glucosides.
摘要翻译：本发明提供编码CYP79家族的细胞色素P450单加氧酶的DNA，其催化脂肪族或芳香酸或链延长的甲硫氨酸同系物转化为相应的肟。本发明的优选实施方案是催化L-缬氨酸和L-异亮氨酸的转化的酶，例如木薯酶CYP79D1和CYP79D2，催化酪氨酸转化的酶，例如Triglochin maritima酶CYP79E1和CYP79E2，催化色氨酸转化为相应的肟吲哚-3-乙醛醛的酶，如拟南芥酶CYP79A2和欧洲油菜酶CYP79B5，以及催化一种链延长的甲硫氨酸同系物，例如拟南芥酶CYP79F1和CYP79F2。所述DNA或其部分在植物中的转基因表达可用于操作相应的硫代葡萄糖苷或氰基葡糖苷的生物合成。

75. 发明申请

WO01012861A1 GENE CLONING 审中-公开
标题翻译：基因克隆
公开(公告)号：WO01012861A1
公开(公告)日：2001-02-22
申请号：PCT/US2000/022743
申请日：2000-08-18
IPC分类号： C12N9/06 , C12N15/10 , C12N15/52 , C12Q1/68
CPC分类号： C12N15/1093 , C12N9/003 , C12N15/52 , C12Q1/6876
摘要： There is provided a method of targeted cloning of genes and gene clusters by directly isolating the DNA of interest from a mixed population, thereby permitting the construction of a very targeted, highly enriched library. Also provided are several unique methods for cloning the genes provided by this method and the probes used in connection with this method.
摘要翻译：提供了通过直接从混合群体中分离感兴趣的DNA来靶向克隆基因和基因簇的方法，从而允许构建非常有针对性的高度富集的文库。还提供了用于克隆由该方法提供的基因和与该方法结合使用的探针的几种独特方法。

76. 发明申请

WO01000797A1 SULFUR ATOM-FREE ENZYME PROTEINS 审中-公开
标题翻译： SUFF无原子免疫酶蛋白
公开(公告)号：WO01000797A1
公开(公告)日：2001-01-04
申请号：PCT/JP2000/002112
申请日：2000-03-31
IPC分类号： C12N9/00 , C12N9/06 , C12N9/08 , C12N9/24 , C12N15/53 , C12N15/56 , C12N9/02 , C12N15/52
CPC分类号： C12N9/003 , C07K2319/00 , C12N9/00 , C12N9/0065 , C12N9/248 , C12Y302/01008
摘要： Sulfur atom-free enzyme proteins sustaining the activity of the intact enzyme proteins and showing an oxidation resistance wherein L-cysteine and L-methionine residues in enzyme proteins have been substituted by 18 L-amino acid residues including L-alanine, L-aspartic acid, L-glutamic acid, L-phenylalanine, L-glycine, L-histidine, L-isoleucine, L-lysine, L-leucine, L-asparagine, L-proline, L-glutamine, L-arginine, L-serine, L-threonine, L-valine, L-tyrosine and L-tryptophan. Namely, enzyme proteins having an antioxidative property against oxidation with hydrogen peroxide, etc. while sustaining the activity of the intact enzymes and a process for producing the same.
摘要翻译：维持完整酶蛋白活性并显示抗氧化性的无硫原子的酶蛋白，其中酶蛋白中的L-半胱氨酸和L-甲硫氨酸残基已被18个L-氨基酸残基取代，包括L-丙氨酸，L-天冬氨酸，L-谷氨酸，L-苯丙氨酸，L-甘氨酸，L-组氨酸，L-异亮氨酸，L-赖氨酸，L-亮氨酸，L-天冬酰胺，L-脯氨酸，L-谷氨酰胺，L-精氨酸，L-丝氨酸， L-苏氨酸，L-缬氨酸，L-酪氨酸和L-色氨酸。即，具有抗过氧化氢氧化的抗氧化性能的酶蛋白，同时维持完整酶的活性及其制备方法。

77. 发明申请

WO00028006A2 PLANT NADP-SPECIFIC GLUTAMATE DEHYDROGENASE 审中-公开
标题翻译：植物NADP特异性谷氨酸脱氢酶
公开(公告)号：WO00028006A2
公开(公告)日：2000-05-18
申请号：PCT/US1999/025949
申请日：1999-11-04
IPC分类号： C12N9/06 , C12N15/82 , C12N9/00
CPC分类号： C12N9/0016 , C12N15/8261 , Y02A40/146
摘要： This invention relates to an isolated nucleic acid fragment encoding an ammonium assimilation protein. The invention also relates to the construction of a chimeric gene encoding all or a portion of the ammonium assimilation protein, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the ammonium assimilation protein in a transformed host cell.
摘要翻译：本发明涉及编码铵同化蛋白的分离的核酸片段。本发明还涉及在正义或反义方向上构建编码全部或部分的铵同化蛋白的嵌合基因，其中嵌合基因的表达导致转化的宿主细胞中的同源化蛋白质水平的改变。

78. 发明申请

WO00024606A1 A COUPLING MECHANISM FOR A FRAMELESS TRAILER 审中-公开
标题翻译：一种无框拖车的联动机构
公开(公告)号：WO00024606A1
公开(公告)日：2000-05-04
申请号：PCT/US1999/025049
申请日：1999-10-22
IPC分类号： C07K14/245 , C07K14/31 , C12N9/06 , C12N9/10 , C12N9/24 , C12N9/78 , C12N9/90 , C12N15/63 , C12Q1/68 , B60P1/18
CPC分类号： C07K14/245 , B60D1/015 , B60P1/283 , B62D53/0842 , C07K14/31 , C12N9/2402 , C12N15/63 , C12Q1/6897
摘要： A coupler is provided for coupling a frameless trailer (10) to a wheel of a tractor. The coupler includes both a coupling plate (38) having a king pin (40) and a pivot member (128). The pivot member (128) pivotably couples the lower end of a hydraulic cylinder (102) to the coupling plate (38). The hydraulic cylinder (104) lifts the front end of the trailer body between a down or "travel" position, and an up or "dump" position. The coupling plate (38) also includes an upper surface on which forwardly extending (202, 204) and rearwardly extending (208, 210) engagement members are placed. The engagement members (202, 204, 208, 210) extend upwardly from the surface of the coupling member, and each include an upper engagement surface (203, 205, 207, 211).
摘要翻译：提供了一种联接器，用于将无框拖车（10）联接到拖拉机的车轮。耦合器包括具有主销（40）和枢转构件（128）的联接板（38）。枢转构件（128）将液压缸（102）的下端可枢转地联接到联接板（38）。液压缸（104）将拖车主体的前端提升到向下或“行进”位置和向上或“倾倒”位置。联接板（38）还包括上表面，其上放置有向前延伸的（202,204）和向后延伸的（208,210）接合构件。接合构件（202,204,208,210）从联接构件的表面向上延伸，并且每个包括上接合表面（203,205,207,211）。

79. 发明申请

WO99063100A1 PROCESS FOR OBTAINING TRANSGENIC PLANTS WHICH HAVE AN IMPROVED CAPACITY FOR THE UPTAKE OF NUTRIENTS AND TOLERANCE TO TOXIC COMPOUNDS WHICH ARE PRESENT IN THE SOIL 审中-公开
标题翻译：具有改善营养物质的能力的转基因植物的过程和对土壤中存在的毒性化合物的耐受性
公开(公告)号：WO99063100A1
公开(公告)日：1999-12-09
申请号：PCT/MX1998/000020
申请日：1998-05-29
IPC分类号： C07H21/04 , C12N9/04 , C12N9/06 , C12N9/88 , C12N15/82 , A01H4/00 , A01H5/00 , A01H5/10 , C12N5/04 , C12P21/06
CPC分类号： C12N9/0028 , C12N9/0006 , C12N9/88 , C12N15/8271
摘要： The invention relates to a process for obtaining through genetic engineering plants with an improved capacity to synthesize, accumulate and exudate organic acids. More specifically, it relates to the generation of transgenic plants which have an improved capacity for the production and excretion of organic acids, providing them with an improved capacity to absorb natural nutrients for the soil or added nutrients such as fertilizers. Said plants have also an incremented capacity to tolerate the presence in the soil of certain toxic compounds such as aluminium. The transformation process implies the introduction of genes which increase the capacity of the plant to produce organic acids and comprises the following steps: a) preparation of a recombinant molecule comprising the coding sequence for an enzyme which produces organic acids, functionally ligated to a promoter sequence which is active in vegetable cells and a terminator of the functional transcription in vegetable cells; b) transformation of vegetable cells with said construction; c) regeneration of transgenic plants from transformed cells.
摘要翻译：本发明涉及通过遗传工程植物获得具有改进的合成，积累和渗出有机酸的能力的方法。更具体地说，它涉及具有改善的有机酸生产和排泄能力的转基因植物的生成，为其提供了吸收土壤天然营养物质或添加营养物质如肥料的改进能力。所述植物还具有增加的容忍在土壤中存在某些有毒化合物如铝的能力。转化过程意味着引入增加植物生产有机酸的能力的基因，并且包括以下步骤：a）制备重组分子，其包含产生有机酸的酶的编码序列，功能性连接到启动子序列其在植物细胞中是活性的，并且在植物细胞中具有功能转录的终止子; b）用所述构造转化植物细胞; c）从转化细胞再生转基因植物。

80. 发明申请

WO99047560A1 ISOFORM 2 OF DIMETHYLGLYCINE DEHYDROGENASE-LIKE GENE 审中-公开
标题翻译：二甲基胆碱脱氢酶基因的ISOFORM 2
公开(公告)号：WO99047560A1
公开(公告)日：1999-09-23
申请号：PCT/CN1998/000041
申请日：1998-03-18
IPC分类号： C12N9/06 , C12N15/12 , C07K14/47 , C12N15/63
CPC分类号： C12N9/0026
摘要： Dimethylglycine dehydrogenase-like 2 polypeptides and polynucleotides and methods for producing such polypeptides by recombinant techniques are disclosed. Also disclosed are methods for utilizing dimethylglycine dehydrogenase-like 2 polypeptides and polynucleotides in the design of protocols for the treatment of sarcosinemia, cardiomyopathy, retinitis pigmentosa, deafness, neurological disease, cancer, metabolic defects and AIDS, among others, and diagnostic assays for such conditions.
摘要翻译：公开了二甲基甘氨酸脱氢酶样2多肽和多核苷酸以及通过重组技术产生此类多肽的方法。还公开了在设计用于治疗肌浆蛋白血症，心肌病，色素性视网膜炎，耳聋，神经疾病，癌症，代谢缺陷和AIDS等的方案的设计中使用二甲基甘氨酸脱氢酶样2多肽和多核苷酸的方法，以及用于其的诊断测定条件。

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