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71. 发明申请

WO2011055266A3 A METHOD OF NUCLEIC ACIDS EXTRACTION FROM MICROORGANISMS IN THE PRESENCE OF METALLIC IONS THAT EMPLOYS PARTICULATED CHITOSAN 审中-公开
标题翻译：在存在金属离子的情况下，微生物提取的核酸提取方法
公开(公告)号：WO2011055266A3
公开(公告)日：2016-08-11
申请号：PCT/IB2010054828
申请日：2010-10-25
申请人： BIOSIGMA SA , REYES JARA ANGÉLICA , PARADA VALDECANTOS PILAR , BADILLA OHLBAUM RICARDO , ROMÁN ESPINOZA ENRIQUE
发明人： REYES JARA ANGÉLICA , PARADA VALDECANTOS PILAR , BADILLA OHLBAUM RICARDO , ROMÁN ESPINOZA ENRIQUE
IPC分类号： C12N1/08
CPC分类号： C12N15/1003
摘要： The present invention describes a method for nucleic acids extraction from microorganisms in the presence of metallic ions that avoids degradation of the nucleic acids obtained. This method includes the following stages: (a) adding particulated chitosan to a suspensión of microorganisms contaminated with metallic ions, (b) incubating this mixture for 10 to 60 minutes with modérate stirring, (c) separating the particulated chitosan from the suspensión of microorganisms, (d) lysing the cells present in the suspensión of the previous stage, and (e) subsequently separating the nucleic acids from the other cell elements in the lysed cells.
摘要翻译：本发明描述了一种在金属离子存在下从微生物中提取核酸的方法，其避免所获得的核酸降解。该方法包括以下阶段：（a）将微粒壳聚糖加入到被金属离子污染的微生物悬浮液中，（b）在搅拌下将该混合物温育10至60分钟，（c）将微粒壳聚糖与微生物悬浮液分离，（d）裂解存在于前一阶段的悬浮液中的细胞，和（e）随后将裂解细胞中的核酸与其它细胞元件分离。

72. 发明申请

WO2016069345A1 SAMPLE PREPARATION VESSELS, MICROFLUIDIC CIRCUITS, AND SYSTEMS AND METHODS FOR SAMPLE PREPARATION, EXTRACTION, AND ANALYSIS 审中-公开
标题翻译：样品制备容器，微流控电路，以及样品制备，萃取和分析的系统和方法
公开(公告)号：WO2016069345A1
公开(公告)日：2016-05-06
申请号：PCT/US2015/056641
申请日：2015-10-21
申请人： ENVIROLOGIX INC.
发明人： SHAFFER, Daniel
IPC分类号： C12N1/08 , C12M3/00 , B01D63/00 , B01D27/00 , C12Q1/68
CPC分类号： C12Q1/6844 , C12Q1/6837 , G01N33/68 , C12Q2565/629
摘要： The invention generally provides a sample preparation vessel including a flexible substrate defining at least one sealable opening adapted and configured to receive a solid sample; at least one fitting; and at least one filter adjacent to the at least one fitting, the filter adapted and configured to permit extracted fluids to exit the vessel while retaining solid particles, as well as vessels, circuits, systems, and related methods for sample preparation, extraction, and analysis.
摘要翻译：本发明通常提供一种样品制备容器，其包括限定至少一个可密封的开口的柔性基底，所述可密封开口适于并构造成容纳固体样品; 至少一个配件; 以及与所述至少一个配件相邻的至少一个过滤器，所述过滤器适于并且构造成允许提取的流体离开所述容器同时保留固体颗粒，以及容器，回路，系统和用于样品制备，提取和分析。

73. 发明申请

WO2013059595A1 REDUCED GENOME BACTERIA WITH IMPROVED GENETIC STABILITY 审中-公开
标题翻译：具有改善的遗传稳定性的减少的基因组细菌
公开(公告)号：WO2013059595A1
公开(公告)日：2013-04-25
申请号：PCT/US2012/061027
申请日：2012-10-19
申请人： SCARAB GENOMICS, LLC
发明人： BLATTNER, Frederick, R. , CSORGO, Balint , POSFAI, Gyorgy
IPC分类号： C12N1/08 , C12N15/10
CPC分类号： C12N15/70 , C12N1/08 , C12N1/20 , C12P21/00 , C12P21/02 , C12R1/19
摘要： Reduced genome bacteria with improved genetic stability are provided. Also provided are methods of producing polypeptides using the reduced genome bacteria with improved genetic stability.
摘要翻译：提供了具有改善的遗传稳定性的减少的基因组细菌。还提供了使用具有改善的遗传稳定性的还原的基因组细菌产生多肽的方法。

74. 发明申请

WO2013043860A1 ENDOGENOUS DNASE ACTIVITY TO REDUCE DNA CONTENT 审中-公开
标题翻译：内源DNASE活性降低DNA含量
公开(公告)号：WO2013043860A1
公开(公告)日：2013-03-28
申请号：PCT/US2012/056315
申请日：2012-09-20
申请人： DANISCO US INC. , HOFFMANN, Katherine , KO, Douglas , WARD, Michael
发明人： HOFFMANN, Katherine , KO, Douglas , WARD, Michael
IPC分类号： C12N1/08 , C12N1/14 , C12P21/02
CPC分类号： C12N1/08 , C12N1/14 , C12N9/16 , C12N9/20 , C12P21/02 , C12Q1/686
摘要： The application provides a method of reducing the DNA content of a protein preparation or a culture broth from a filamentous fungal host cell using an endogenous filamentous fungal host DNase activity.
摘要翻译：该应用提供了使用内源丝状真菌宿主DNA酶活性从丝状真菌宿主细胞中降低蛋白质制剂或培养肉汤的DNA含量的方法。

75. 发明申请

WO2012054613A3 STABILIZED PROTEASE-CONTAINING SOLUTIONS 审中-公开
标题翻译：稳定的含蛋白酶的溶液
公开(公告)号：WO2012054613A3
公开(公告)日：2012-06-14
申请号：PCT/US2011056900
申请日：2011-10-19
申请人： BIO RAD LABORATORIES , OKINO STEVEN T , WANG YAN
发明人： OKINO STEVEN T , WANG YAN
IPC分类号： C12N1/08
CPC分类号： C12N9/22 , C12N9/58 , C12N9/96 , C12N15/1003 , C12Y301/27005 , C12Y304/21064
摘要： The present invention provides compositions and methods for purifying RNA-free DNA from a sample. An exemplary reagent mixture for purifying RNA-free DNA from a sample comprising DNA, RNA, and protein, the reagent mixture comprising: a protease; an RNase; and a detergent, wherein the protease in the reagent mixture does not substantially inactivate the RNase when the mixture is stored for at least 24 hours at 4°C.
摘要翻译：本发明提供了用于从样品中纯化无RNA的DNA的组合物和方法。用于从包含DNA，RNA和蛋白质的样品中纯化无RNA的DNA的示例性试剂混合物，所述试剂混合物包含：蛋白酶; 核糖核酸酶和洗涤剂，其中当混合物在4℃下储存至少24小时时，试剂混合物中的蛋白酶基本上不使核糖核酸酶失活。

76. 发明申请

WO2008066858A2 METHOD OF SEPARATING TARGET DNA FROM MIXED DNA 审中-公开
标题翻译：从混合DNA分离靶标DNA的方法
公开(公告)号：WO2008066858A2
公开(公告)日：2008-06-05
申请号：PCT/US2007/024523
申请日：2007-11-29
申请人： CANON U.S. LIFE SCIENCES, INC. , STONE, Michelle
发明人： STONE, Michelle
IPC分类号： C12N1/08
CPC分类号： C12N15/1003 , C12N15/1017
摘要： The present invention relates to methods of separating target DNA from mixed DNA in a sample. In some embodiments, the target DNA is present in target organisms. In other embodiments, the target organisms may be viruses, bacteria, fungi or combinations thereof. In some embodiments the sample contains cells having nuclei. In some embodiments the cells are mammalian cells. In some embodiments, the separated target organisms are treated to release their DNA which can be recovered.
摘要翻译：本发明涉及从样品中的混合DNA中分离靶DNA的方法。在一些实施方案中，靶DNA存在于靶生物中。在其它实施方案中，靶生物可以是病毒，细菌，真菌或其组合。在一些实施方案中，样品含有具有核的细胞。在一些实施方案中，细胞是哺乳动物细胞。在一些实施方案中，处理分离的靶生物以释放其可以回收的DNA。

77. 发明申请

WO2006086210A2 METHODS AND COMPOSITIONS FOR TAGGING AND IDENTIFYING POLYNUCLEOTIDES 审中-公开
标题翻译：用于标记和鉴定多核苷酸的方法和组合物
公开(公告)号：WO2006086210A2
公开(公告)日：2006-08-17
申请号：PCT/US2006/003689
申请日：2006-02-03
申请人： COMPASS GENETICS, LLC , BRENNER, Sydney
发明人： BRENNER, Sydney
IPC分类号： C12Q1/68 , C12P19/34 , C12N1/08
CPC分类号： C12Q1/6874 , C12N15/1065 , C12N15/1093 , C12P19/34 , C12Q1/6827 , C12Q1/6837 , Y10T436/143333 , C12Q2565/514 , C12Q2563/179 , C12Q2537/143 , C12Q2525/191
摘要： The invention provides methods and compositions for attaching oligonucleotide tags to polynucleotides for the purpose of carrying out analytical assays in parallel and for decoding the oligonucleotide tags of polynucleotides selected in such assays. Words, or subunits, of oligonucleotide tags index submixtures in successively more complex sets of submixtures (referred to herein as "tiers" of submixtures) that a polynucleotide goes through while successive words are added to a growing tag. By identifying each word of an oligonucleotide tag, a series of submixtures is identified including the first submixture that contains only a single polynucleotide, thereby providing the identity of the selected polynucleotide. The analysis of the words of an oligonucleotide tag can be carried out in parallel, e.g. by specific hybridization of the oligonucleotide tag to its tag complement on an addressable array; or such analysis can be carried out serially by successive specific hybridizations of labeled word complements, or the like.
摘要翻译：本发明提供了用于将寡核苷酸标签连接到多核苷酸的方法和组合物，用于并行进行分析测定并解码在这些测定中选择的多核苷酸的寡核苷酸标签。寡核苷酸标签的单词或亚单位在连续更复杂的一组子混合物（本文中称为“子混合物”）中指示子混合物，多核苷酸经过，而连续词被添加到生长标签中。通过鉴定寡核苷酸标签的每个单词，鉴定了一系列的亚混合物，其包括仅包含单个多核苷酸的第一个亚基混合物，从而提供所选多核苷酸的身份。寡核苷酸标签的词的分析可以并行进行，例如，通过在可寻址阵列上寡核苷酸标签与其标签补体的特异性杂交; 或者这样的分析可以通过标记的词互补体的连续特异性杂交等连续进行。

78. 发明申请

WO2006060282A3 MIXING DEVICES FOR CHEMICAL LYSIS OF CELLS 审中-公开
公开(公告)号：WO2006060282A3
公开(公告)日：2006-06-08
申请号：PCT/US2005/042797
申请日：2005-11-28
申请人： MERIAL LIMITED , DETRAZ, Noël, Joseph, François , RIGAUT, Guillaume
发明人： DETRAZ, Noël, Joseph, François , RIGAUT, Guillaume
IPC分类号： C12M1/33 , C12N1/06 , C12N1/08 , C12P1/00 , C12M1/34 , C12M1/36 , C12M1/38
摘要： The invention relates to flow-through devices for mixing fluids and uses thereof for mixing and for lysing cells to release biological compounds of interest. The invention particularly encompasses flow-through methods for mixing and chemical methods for the isolation and purification of plasmid DNA from cell culture.

79. 发明申请

WO2006034009A2 PURIFICATION OF BIOMOLECULES FROM CONTAMINATING INTACT NUCLEIC ACIDS 审中-公开
标题翻译：生物分子的净化从污染的核酸中分离出来
公开(公告)号：WO2006034009A2
公开(公告)日：2006-03-30
申请号：PCT/US2005/033091
申请日：2005-09-14
申请人： SIGMA-ALDRICH CO. , WARD, Brian Walter , BUNTAINE, Brian Ward
发明人： WARD, Brian Walter , BUNTAINE, Brian Ward
IPC分类号： C12Q1/68 , C12N1/08
CPC分类号： C12N9/1252 , C12N1/08 , C12N9/1241
摘要： The present invention provides methods for the removal, destruction or inactivation of intact nucleic acids contaminating desired biomolecules by the use of small molecule nucleic acid cleavage agents.
摘要翻译：本发明提供了通过使用小分子核酸切割剂去除，破坏或灭活污染所需生物分子的完整核酸的方法。

80. 发明申请

WO2004042003A3 CELL LYSIS COMPOSITIONS, METHODS OF USE, APPARATUS, AND KIT 审中-公开
标题翻译：细胞裂解组合物，使用方法，装置和试剂盒
公开(公告)号：WO2004042003A3
公开(公告)日：2004-08-12
申请号：PCT/US0330463
申请日：2003-09-26
申请人： PROMEGA CORP , ENGEL LAURIE , SHULTZ JOHN W , JOHNSON TONNY M , ZIMMERMAN KRISTOPHER , BOZEK LAURA L , STEVENS JUDITH N
发明人： ENGEL LAURIE , SHULTZ JOHN W , JOHNSON TONNY M , ZIMMERMAN KRISTOPHER , BOZEK LAURA L , STEVENS JUDITH N
IPC分类号： B01D15/36 , B01D15/38 , C07H19/00 , C07H21/00 , C07K1/36 , C11D1/44 , C11D1/66 , C11D1/72 , C11D1/88 , C11D3/37 , C11D3/38 , C11D3/386 , C12N20060101 , C12N1/06 , C12N1/08 , C12P21/06
CPC分类号： B01D15/3804 , B01D15/361 , B01J20/28009 , C07K1/36 , C11D1/44 , C11D1/662 , C11D1/72 , C11D1/88 , C11D3/3719 , C11D3/381 , C11D3/38636 , C12N1/06
摘要： Cell lysis compositions, methods for extracting and isolating proteins and peptides from a host cells using the compositions, kits and apparatus for extracting and isolating protein and peptide molecules from host cells and for detecting for the presence of a protein or peptide. The composition allows for the extraction and isolation of proteins and peptides from host cells without the need for mechanical disruption and with or without isolation of the cells from cell medium. The composition includes at least one surfactant having a hydrophobic-lipophilic balance value in the range from about 11 to about 16; and at least one cell membrane altering compound.
摘要翻译：细胞裂解组合物，使用所述组合物，试剂盒和设备从宿主细胞中提取和分离蛋白质和肽以及从宿主细胞中提取和分离蛋白质和肽分子并检测蛋白质或肽的存在的方法。该组合物允许从宿主细胞中提取和分离蛋白质和肽，而不需要机械破坏以及有或没有从细胞培养基中分离细胞。该组合物包括至少一种疏水 - 亲油平衡值在约11至约16范围内的表面活性剂; 和至少一种改变细胞膜的化合物。

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