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    • 62. 发明申请
    • ENZYMATIC METHODS FOR POLYUNSATURATED FATTY ACID ENRICHMENT
    • 用于聚脂肪脂肪酸增强的酶学方法
    • WO1996026287A1
    • 1996-08-29
    • PCT/FR1996000226
    • 1996-02-13
    • SOBREMAR S.A.BRETON, Gildas
    • SOBREMAR S.A.
    • C12P07/64
    • C12P7/6427C11C1/045C11C3/02C11C3/10C12P7/6472C12P7/6481
    • A method for use in the field of chemistry and particularly in the chemistry of fats. The method is used to prepare polyunsaturated fatty acid concentrates and comprises selectively enzymatically hydrolysing a fish oil containing docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) in position 1, 2 or 3, to give a mixture of free fatty acids, monoglycerides and diglycerides, separating the components of the mixture, recovering the free fatty acids and purifying them by urea crystallisation to increase the EPA and DHA content, decomplexing the isolated fatty acids, and performing interesterification between the free fatty acids having a high polyunsaturated fatty acid concentration and the unprocessed oil, in the presence of a position- or steric hindrance-specific lipase, to give a polyunsaturated fatty acid triglyceride-enriched mixture which is separated while the free fatty acids are removed therefrom. A method for enzymatically enriching phospholipids with polyunsaturated fatty acids (EPA, DHA), and the synthesis of polyunsaturated fatty acid monoacylglycerols of series n3 by enzymatic synthesis using 1,2-dialkylene glycerol as the starting material, are also disclosed. The resulting products are suitable for use in foodstuffs, cosmetics and pharmaceuticals.
    • 一种用于化学领域,特别是脂肪化学领域的方法。 该方法用于制备多不饱和脂肪酸浓缩物,并且包括选择性酶促水解1,2或3位的二十二碳六烯酸(DHA)和二十碳五烯酸(EPA)的鱼油,得到游离脂肪酸,甘油单酯和甘油二酯的混合物 ,分离混合物的组分,回收游离脂肪酸并通过尿素结晶纯化,以增加EPA和DHA含量,使分离的脂肪酸解链,并进行具有高多不饱和脂肪酸浓度的游离脂肪酸和 在处于位置或空间位阻特异性脂肪酶的情况下,未加工的油,得到富含多不饱和脂肪酸甘油三酸酯的混合物,其中游离脂肪酸被除去。 还公开了使用多不饱和脂肪酸(EPA,DHA)酶促富集磷脂的方法,以及通过使用1,2-二亚烷基甘油作为起始原料的酶合成合成n3系列的多不饱和脂肪酸单酰基甘油的方法。 所得产品适用于食品,化妆品和药品。
    • 65. 发明申请
    • METHOD FOR PREPARING 2-MONOACYLGLYCERIDES
    • 制备2-单乙酰胆碱的方法
    • WO2017003992A1
    • 2017-01-05
    • PCT/US2016/039728
    • 2016-06-28
    • STEPAN COMPANY
    • RONGIONE, Joseph, C.
    • C11C1/00C11C1/04C11C1/08C11C1/10
    • C11C1/045C07C31/08C07C47/54C07C47/548C07C47/58C11C1/08C11C1/10C12N9/20C12P7/6445C12Y301/01003
    • Methods for preparing and purifying 2-monoacylglyceride compounds are disclosed. In one method, an unsaturated triglyceride is reacted with water, a C 1 -C 8 alcohol, or a mixture thereof in the presence of a lipase to produce a mixture comprising a 1,3-dihydroxy-2-monoacylglyceride and fatty esters or acids. Reaction of the 1,3-dihydroxy-2-monoacylglyceride with an aldehyde or ketone gives a mixture comprising a 2-monoacylglyceride acetal or ketal. Fatty esters or acids are removed from the mixture as an overhead product by distillation or wiped-film evaporation to isolate a purified 2-monoacylglyceride acetal or ketal. The inventive methods provide a 2-monoacylglyceride protected at the 1 - and 3- positions such that the acyl unit remains at the 2- position. The products are enriched in unsaturated fatty acid content when compared with the unsaturated fatty acid content of the original unsaturated triglyceride. Each method utilizes a practical purification scheme that avoids the scale-up or toxicity issues of commonly employed purification strategies.
    • 公开了制备和纯化2-单酰基甘油酯化合物的方法。 在一种方法中,不饱和甘油三酸酯在脂肪酶存在下与水,C 1 -C 8醇或其混合物反应,以产生包含1,3-二羟基-2-单酰基甘油酯和脂肪酸酯或酸的混合物。 1,3-二羟基-2-单酰基甘油酯与醛或酮的反应得到包含2-单酰基甘油酯缩醛或缩酮的混合物。 通过蒸馏或擦拭膜蒸发将脂肪酯或酸作为塔顶产物从混合物中除去以分离纯化的2-单酰基甘油酯缩醛或缩酮。 本发明的方法提供在1-和3-位保护的2-单酰基甘油酯,使得酰基单元保留在2-位。 与原始不饱和甘油三酸酯的不饱和脂肪酸含量相比,产品富含不饱和脂肪酸含量。 每种方法都采用一种实用的纯化方案,可以避免常用纯化策略的放大或毒性问题。