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51. 发明申请

WO99002654A1 ISOLATION OF BONE AND CARTILAGE PRECURSOR CELLS 审中-公开
标题翻译：骨髓和前列腺细胞的分离
公开(公告)号：WO99002654A1
公开(公告)日：1999-01-21
申请号：PCT/US1998/014587
申请日：1998-07-14
IPC分类号： G01N33/53 , A61K31/00 , A61K35/12 , A61K35/14 , A61K35/28 , A61K38/00 , A61K38/17 , A61K47/02 , A61K47/12 , A61K47/34 , A61K47/42 , A61L27/38 , A61P19/00 , A61P19/08 , A61P43/00 , C07K16/28 , C12N5/077 , C12N5/0775 , C12N5/0789 , C12N15/09 , C12P21/08 , G01N33/48 , C12N5/00
CPC分类号： C12N5/0667 , A61K35/12 , A61K38/00 , A61K2035/124 , A61L27/3817 , A61L27/3821 , A61L27/3839 , A61L27/3847 , A61L27/3852 , C12N5/0663 , C12N2500/38 , C12N2501/39 , C12N2501/91
摘要： The present invention relates to the isolation of cartilage or bone precursor cells from hematopoietic and non-hematopoietic cells and their use in bone and cartilage regeneration procedures. The precursor cells are used for in vivo bone or cartilage repair by transplanting the cells, with or without a carrier material and without the need for in vitro culturing of the cells, to sites in the body requiring bone or cartilage repair.
摘要翻译：本发明涉及从造血细胞和非造血细胞中分离软骨或骨前体细胞及其在骨和软骨再生过程中的用途。前体细胞用于体内骨或软骨修复，通过移植具有或不具有载体材料的细胞，并且不需要体外培养细胞到需要骨或软骨修复的身体中的部位。

52. 发明申请

WO1997044442A1 ISOLATION, PROPAGATION, AND DIRECTED DIFFERENTIATION OF STEM CELLS FROM CENTRAL NERVOUS SYSTEM OF MAMMALS 审中-公开
标题翻译：干细胞从中央神经系统分离，传播和指导的差异
公开(公告)号：WO1997044442A1
公开(公告)日：1997-11-27
申请号：PCT/US1997007669
申请日：1997-05-07
申请人： NEURALSTEM BIOPHARMACEUTICALS , JOHE, Karl, K.
发明人： NEURALSTEM BIOPHARMACEUTICALS
IPC分类号： C12N05/08
CPC分类号： G01N33/5073 , A61K35/12 , C12N5/0623 , C12N2500/90 , C12N2501/11 , C12N2501/115 , C12N2501/13 , C12N2501/148 , C12N2501/395 , C12N2501/91 , C12N2506/02 , C12N2510/02 , G01N33/5008 , G01N33/502 , G01N33/5058 , G01N33/9413
摘要： The present invention reveals an in vitro procedure by which an homogeneous population of multipotential precursor cells from mammalian embryonic neuroepithelium (CNS stem cells) can be expanded up to 10 fold in culture while maintaining their multipotential capacity to differentiate into neurons, oligodendrocytes, and astrocytes. Chemically defined conditions are presented that enable a large number of neurons, up to 50 % of the expanded cells, to be derived from the stem cells. In addition, four factors -- PDGF, CNTF, LIF and T3 -- have been identified, which, individually, generate significantly higher proportion of neurons, astrocytes, or oligodendrocytes. These defined procedures permit a large-scale preparation of the mammalian CNS stem cells, neurons, astrocytes, and oligodendrocytes under chemically defined conditions with efficiency and control. The present invention also reveals in vitro cultures of region-specific, terminally differentiated, mature neurons derived from cultures of mammalian multipotential CNS stem cells and an in vitro procedure by which the differentiated neurons may be generated.
摘要翻译：本发明揭示了一种体外方法，其中来自哺乳动物胚胎神经上皮（CNS干细胞）的多潜能前体细胞的均质群体可以在培养物中扩增至10 9倍，同时保持其多能力分化为神经元，少突胶质细胞，和星形胶质细胞。提出了化学上定义的条件，使得能够从干细胞中获得多达50％的扩增细胞的大量神经元。此外，已经鉴定了四个因子 - PDGF，CNTF，LIF和T3-个体，其分别产生显着更高比例的神经元，星形胶质细胞或少突胶质细胞。这些定义的方法允许在化学定义的条件下大量制备哺乳动物CNS干细胞，神经元，星形胶质细胞和少突胶质细胞，其效率和对照。本发明还揭示衍生自哺乳动物多潜能CNS干细胞培养物的区域特异性，终末分化的成熟神经元的体外培养物，以及可产生分化的神经元的体外方法。

53. 发明申请

WO1997026326A1 ISOLATION OF PRECURSOR CELLS FROM HEMATOPOIETIC AND NON-HEMATOPOIETIC TISSUES AND THEIR USE 审中-公开
标题翻译：从HEMATOPOIETIC和非HEMATOPOIETIC TISSUES及其使用中分离前列腺细胞
公开(公告)号：WO1997026326A1
公开(公告)日：1997-07-24
申请号：PCT/US1997001213
申请日：1997-01-13
申请人： BOEHRINGER MANNHEIM CORPORATION
发明人： BOEHRINGER MANNHEIM CORPORATION , PETERSON, Dale, R. , NOUSEK-GOEBL, Nancy
IPC分类号： C12N05/00
CPC分类号： A61L27/3821 , A61K35/12 , A61K38/00 , A61K2035/124 , A61L27/3817 , A61L27/3839 , A61L27/3847 , A61L27/3852 , C12N5/0663 , C12N5/0665 , C12N5/0667 , C12N2500/38 , C12N2501/39 , C12N2501/91
摘要： Subsets of cells from hematopoietic and non-hematopoietic tissues are used for bone and cartilage regeneration. Bone and cartilage precursor cells are isolated from peripheral blood, bone marrow and adipose tissue using antibodies and other reagents that recognize hematopoietic cell surface marker CD34 and other markers on CD34+ cells. Precurosr cells may be used for in vivo bone or cartilage regeneration by transplanting the cells with or without a carrier material to sides in the body requiring bone or cartilage repair without the need for in vitro culturing. Precursor cells may also be used to seed prosthetic devices to enhance implantability.
摘要翻译：来自造血和非造血组织的细胞亚群用于骨和软骨再生。使用识别造血细胞表面标志物CD34和CD34 +细胞上的其他标记物的抗体和其它试剂从外周血，骨髓和脂肪组织分离骨和软骨前体细胞。通过将具有或不具有载体材料的细胞移植到身体中需要骨或软骨修复的侧面而不需要体外培养，可将Precurosr细胞用于体内骨或软骨再生。前体细胞也可用于种植假体装置以增强植入性。

54. 发明申请

WO1997024434A1 EXPANSION OF BONE MARROW STROMAL CELLS 审中-公开
标题翻译：骨巨细胞扩张
公开(公告)号：WO1997024434A1
公开(公告)日：1997-07-10
申请号：PCT/US1995017073
申请日：1995-12-29
申请人： ALG COMPANY
发明人： ALG COMPANY , GREENBERGER, Joel, S. , HURWITZ, David, R.
IPC分类号： C12N05/00
CPC分类号： C12N5/0669 , C12N2501/113 , C12N2501/91
摘要： The invention features a method for the selection and expansion of bone marrow stromal cells. The method includes the steps of obtaining bone marrow stromal cells, introducing the stromal cells into a vessel precoated on an inner surface with a gelatin, and containing a culture medium including an acidic fibroblast growth factor ("aFGF") polypeptide, and expanding the stromal cells in the culture medium under conditions and for a time sufficient to obtain an increased number of bone marrow stromal cells. The culture medium additionally can include heparin, and the vessel additionally can be precoated with fetal bovine serum.
摘要翻译：本发明的特征在于选择和扩增骨髓基质细胞的方法。该方法包括以下步骤：获得骨髓基质细胞，将基质细胞导入预先用明胶预涂在内表面上的容器中，并含有包含酸性成纤维细胞生长因子（“aFGF”）多肽的培养基，并扩展基质在足以获得增加数目的骨髓基质细胞的条件下和一段时间内在培养基中的细胞。培养基另外可以包括肝素，另外还可以用胎牛血清预涂。

55. 发明申请

WO1997024144A1 METHODS OF PREPARING BONE MARROW STROMAL CELLS FOR USE IN GENE THERAPY 审中-公开
标题翻译：用于基因治疗的骨髓基质细胞的制备方法
公开(公告)号：WO1997024144A1
公开(公告)日：1997-07-10
申请号：PCT/US1995016991
申请日：1995-12-29
申请人： ALG COMPANY
发明人： ALG COMPANY , GREENBERGER, Joel, S. , HURWITZ, David, R.
IPC分类号： A61K48/00
CPC分类号： C12N5/0669 , A61K48/00 , C12N2501/113 , C12N2501/91
摘要： This invention relates to sequential methods of cryopreserving bone marrow stromal cells that are transfected and used for gene therapy by transplantation. These methods include the following steps in various orders: obtaining the cells, expanding the cells in culture, transfecting the cells, and cryopreserving the cells. With these methods, populations of bone marrow stromal cells can be acquired that are large enough to be useful in a number of therapies. Further, these large populations can be stored for extended periods of time for immediate use when needed.
摘要翻译：本发明涉及通过移植转染和用于基因治疗的冷冻保存骨髓基质细胞的顺序方法。这些方法包括以下各步骤：获得细胞，扩增培养细胞，转染细胞和冷冻保存细胞。通过这些方法，可以获得足够大的骨髓基质细胞群以在许多疗法中有用。此外，这些大群体可以长时间储存，以便在需要时立即使用。

56. 发明申请

WO2016100035A1 SUSPENSION CULTURING OF PLURIPOTENT STEM CELLS 审中-公开
标题翻译：悬浮培养大肠杆菌干细胞
公开(公告)号：WO2016100035A1
公开(公告)日：2016-06-23
申请号：PCT/US2015/064713
申请日：2015-12-09
申请人： JANSSEN BIOTECH, INC.
发明人： FRYERS, Benjamin , LANIAUSKAS, Daina
IPC分类号： C12N5/071 , C12N5/02
CPC分类号： C12N5/0676 , C12N2500/02 , C12N2500/25 , C12N2500/38 , C12N2500/60 , C12N2501/117 , C12N2501/155 , C12N2501/16 , C12N2501/19 , C12N2501/385 , C12N2501/395 , C12N2501/41 , C12N2501/415 , C12N2501/727 , C12N2501/91 , C12N2501/999 , C12N2506/02
摘要： The present invention provides methods of differentiating pluripotent cells into beta cell using suspension clustering. The methods of the invention use control of one or more of pH, cell concentration, and retinoid concentration to generate a nearly homogenous population of PDX1/NKX6.1 co-expressing cells by suppressing precocious NGN3 expression and promoting NKX6.1 expression. Also, the nearly homogenous population of PDX1/NKX6.1 co-expressing cells may be further differentiated in vitro to form a population of pancreatic endocrine cells that co-express PDX1, NKX6.1, insulin and MAFA.
摘要翻译：本发明提供了使用悬浮聚类将多能细胞分化成β细胞的方法。本发明的方法是通过抑制早期的NGN3表达和促进NKX6.1表达来控制pH，细胞浓度和类视色素浓度中的一种或多种，以产生几乎均匀的PDX1 / NKX6.1共表达细胞群。此外，PDX1 / NKX6.1共表达细胞的几乎均匀的群体可以在体外进一步分化以形成共表达PDX1，NKX6.1，胰岛素和MAFA的胰腺内分泌细胞群体。

57. 发明申请

WO2016032152A1 성상세포의 생산방법 审中-公开
标题翻译：生产ASTROCYTES的方法
公开(公告)号：WO2016032152A1
公开(公告)日：2016-03-03
申请号：PCT/KR2015/008482
申请日：2015-08-13
申请人： 한국생명공학연구원
发明人： 한백수 , 이상철 , 배광희 , 이천옥 , 김원곤 , 오경진
IPC分类号： C12N5/079
CPC分类号： C12N5/0622 , C12N2500/32 , C12N2501/10 , C12N2501/105 , C12N2501/11 , C12N2501/115 , C12N2501/13 , C12N2501/15 , C12N2501/155 , C12N2501/16 , C12N2501/91 , C12N2501/999 , C12N2506/02 , C12N2533/90
摘要： 본 발명은 높은 순도와 동일한 형질의 성상세포를 지속적으로 생산할 수 있도록 줄기세포로부터 신경전구세포를 형성한 다음, 상기 신경전구세포를 2단계에 걸쳐 성상세포로 분화시키는 단계를 포함하는 성상세포의 생산방법 및 상기 방법으로 생산된 성상세포에 관한 것이다. 본 발명에서 제공하는 성상세포의 생산방법을 이용하면, 높은 순도로 성상세포를 생산할 수 있고, 동일한 형질의 성상세포를 보다 빠르게 생산할 수 있을 뿐만 아니라 신경전구세포를 사용하여 필요할 때에 신속하게 성상세포를 분화시킬 수 있으므로, 성상세포의 이식이 필요한 질환이 발병된 환자의 효과적인 치료에 널리 활용될 수 있을 것이다.
摘要翻译：本发明涉及通过该方法生产星形胶质细胞和星形胶质细胞的方法，其中所述方法包括从干细胞形成神经前体细胞的步骤，以连续产生具有高纯度和相同性状的星形胶质细胞，然后区分神经前体细胞通过两个阶段进入星形胶质细胞。使用本发明提供的星形胶质细胞的方法可以产生高纯度的星形胶质细胞; 可以快速产生具有相同特征的星形胶质细胞; 并且可以根据需要迅速地将神经前体细胞分化成星形胶质细胞。因此，本发明的方法可广泛用于有效治疗需要移植星形胶质细胞的疾病患者。

58. 发明申请

WO2015167401A1 HEPARAN SULPHATES 审中-公开
标题翻译： HEPARAN硫酸盐
公开(公告)号：WO2015167401A1
公开(公告)日：2015-11-05
申请号：PCT/SG2015/050092
申请日：2015-04-30
申请人： AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH
发明人： COOL, Simon , NURCOMBE, Victor , LEE, Jonathan
IPC分类号： C08B37/10 , C12P19/04 , C07K14/495 , A61K31/727 , A61P19/08 , A61P17/02 , A61L27/10 , C12N5/077 , A01N1/00
CPC分类号： A61L27/20 , A01N1/0226 , A61K8/735 , A61K31/727 , A61L15/44 , A61L15/60 , A61L27/34 , A61L27/3834 , A61L27/52 , A61L27/54 , A61L2300/414 , A61L2420/02 , A61L2430/06 , C07K14/495 , C08B37/0075 , C12N5/0662 , C12N2501/15 , C12N2501/91 , C08L5/10
摘要： A heparan sulphate that binds ΤGΡβ1 is disclosed.
摘要翻译：公开了结合ΤGPβ1的硫酸乙酰肝素。

59. 发明申请

WO2014135924A1 MENSTRUAL STEMS CELLS FOR THE EFFICIENT SUPPORT AND EXPANSION OF CD34+ CD133+ HEMATOPOIETIC STEM CELLS IN VITRO 审中-公开
标题翻译：用于有效支持和延长体外CD34 + CD133 + HEMATOPOIETIC干细胞的淋巴细胞
公开(公告)号：WO2014135924A1
公开(公告)日：2014-09-12
申请号：PCT/IB2013/051861
申请日：2013-03-08
申请人： CELLS FOR CELLS , KHOURY, Maroun , ALCAYAGA-MIRANDA, Francisca
发明人： KHOURY, Maroun , ALCAYAGA-MIRANDA, Francisca
IPC分类号： C12N5/0787
CPC分类号： C12N5/0647 , C12N5/0665 , C12N2501/06 , C12N2501/105 , C12N2501/113 , C12N2501/125 , C12N2501/145 , C12N2501/91 , C12N2502/243
摘要： The invention relates to highly proliferative stem cell obtained from menstrual fluid. The menstrual stem cells (MenSCs) may be cultured in vitro and exhibit mesenchymal stem cell (MSC)-like properties and some culture and expansion advantages. This population of Men SCs, compared to the broadly studied bone marrow derived stem cells (BM-MSCs) out- performs bone marrow derived mesenchymal stem cells in proliferation rate and support of hematopoietic stem cell (HSC) expansion in vitro. MenSCs demonstrate to maintain their stem cell properties over 2 years, being genetically stable, without expressing surface differentiation markers, and they also show the ability to differentiate into adipocytes, chondrocytes and osteoblast cells. MenSCs can be easily and periodically obtained, isolated and cultured.
摘要翻译：本发明涉及从月经流体获得的高增殖性干细胞。月经干细胞（MenSCs）可以在体外培养并显示出间充质干细胞（MSC）样的特性和一些培养和扩增的优点。与广泛研究的骨髓来源的干细胞（BM-MSC）相比，这种男性SCs群体的增殖速率和体外造血干细胞（HSC）扩增的支持超过了骨髓来源的间充质干细胞。 MenSCs证明维持其干细胞特性2年以上，遗传稳定，不表达表面分化标志物，并且还显示分化成脂肪细胞，软骨细胞和成骨细胞的能力。 MenSCs可以容易地和定期地获得，分离和培养。

60. 发明申请

WO2014076200A1 CELL CULTURE SUPPLEMENTS 审中-公开
标题翻译：细胞培养补品
公开(公告)号：WO2014076200A1
公开(公告)日：2014-05-22
申请号：PCT/EP2013/073865
申请日：2013-11-14
申请人： BIORIGEN INTERNATIONAL SA
发明人： MURAGLIA, Anita , MASTROGIACOMO, Maddalena , CANCEDDA, Ranieri
IPC分类号： C12N5/00
CPC分类号： C12N5/0037 , C12N2500/84 , C12N2501/91 , C12N2502/115
摘要： The invention relates to cell culture supplements based on a platelet rich plasma fraction and a platelet poor plasma fraction. The supplements increase cell proliferation rate, improve selection of clonogenic cells, proliferation of cells from biopsies from elderly patients, maintenance of cell differentiation potential, and in vitro expansion of cell cultures also starting from an extremely low number of initially plated cells. The supplements may be freeze-dried and kept for long period of time as a quality controlled "off the shelf" product.
摘要翻译：本发明涉及基于富含血小板的血浆级分和血小板不良血浆级分的细胞培养补充物。增加细胞增殖率，改善克隆形成细胞的选择，来自老年患者的活组织检查细胞的增殖，细胞分化潜能的维持以及细胞培养物的体外扩增，也从极少数的初始铺板细胞开始。补充品可以冷冻干燥并长期保存，作为质量控制的“现成”产品。

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