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41. 发明申请

WO01032929A1 METHODS AND COMPOSITIONS FOR ANALYSIS OF SNPS AND STRS 审中-公开
标题翻译： SNPS和STRS分析的方法和组合
公开(公告)号：WO01032929A1
公开(公告)日：2001-05-10
申请号：PCT/US2000/030534
申请日：2000-11-03
IPC分类号： C12Q1/68
CPC分类号： C12Q1/683 , C12Q1/6837 , C12Q1/6858 , C12Q2537/119 , C12Q2535/125 , C12Q2525/125 , C12Q2565/519
摘要： The present invention is directed to methods and compositions for detecting differences in sequences, such as genomic sequences. Particularly, the invention is directed to determinations of single nucleotide polymorphisms and short tandem repeats. Methods of detection include triplex protection assays and restriction fragment target assays.
摘要翻译：本发明涉及用于检测诸如基因组序列之类的序列差异的方法和组合物。特别地，本发明涉及单核苷酸多态性和短串联重复序列的测定。检测方法包括三重保护测定和限制性片段靶标测定。

42. 发明申请

WO0050644A3 METHODS FOR PURIFYING DNA USING IMMOBILIZED CAPTURE PROBES 审中-公开
标题翻译：使用固定捕获探针来净化DNA的方法
公开(公告)号：WO0050644A3
公开(公告)日：2001-04-05
申请号：PCT/US0004939
申请日：2000-02-25
申请人： MOSAIC TECHNOLOGIES
发明人： ADAMS CHRISTOPHER P , BOLES T CHRISTIAN , WEIR LAWRENCE , DHANDA RAHUL K , SUMMERS NEVIN M
IPC分类号： C12N15/10 , C12Q1/68 , G01N1/34 , G01N35/10 , B01L3/02
CPC分类号： G01N1/405 , C12N15/101 , C12Q1/6806 , C12Q1/6869 , G01N2035/1053 , C12Q2565/519 , C12Q2565/125
摘要： Methods employing purification devices comprising an electrophoretic medium containing immobilized capture probes for the purification of DNA are disclosed.
摘要翻译：公开了使用包含含有用于纯化DNA的固定化捕获探针的电泳培养基的纯化装置的方法。

43. 发明申请

WO01011080A1 A PRINTED CIRCUIT BOARD, BIOSENSOR AND METHOD OF USING SAME 审中-公开
标题翻译：印刷电路板，生物传感器及其使用方法
公开(公告)号：WO01011080A1
公开(公告)日：2001-02-15
申请号：PCT/US1999/017620
申请日：1999-08-04
IPC分类号： C12Q1/00 , C12Q1/68 , G01N33/543 , C12Q1/26 , C12Q1/28 , C12Q1/42 , G01N27/327
CPC分类号： C12Q1/6825 , B01J2219/00653 , B01J2219/00722 , B01J2219/00729 , C12Q1/001 , C12Q1/6804 , G01N33/5438 , C12Q2565/607 , C12Q2565/531 , C12Q2563/113 , C12Q2565/525 , C12Q2565/519
摘要： The invention, in its various aspects and embodiments, is a printed circuit board biosensor and a use for the same. The printed circuit board biosensor comprises a printed circuit board and a bioreporter. The printed circuit board includes a working electrode and a reference electrode formed thereon. The bioreporter is operably linked to the working electrode and capable of generating an electrochemical signal upon specifically recognizing a target molecule to be detected in a sample when subjected to an electrical potential applied across the working and reference electrodes. The printed circuit board biosensor may, in some embodiments, comprise part of a system for detecting a target molecule in a sample. Such a system might include, in addition to the biosensor, means for detecting the electrochemical signal when a potential is applied across at least one reference electrode and at least one working electrode and/or means for applying the electrical potential. The printed circuit board, and systems including the same, may also comprise kits when sold with instructions on their use in accordance with the present invention.
摘要翻译：本发明在其各个方面和实施例中是印刷电路板生物传感器及其用途。印刷电路板生物传感器包括印刷电路板和生物传送器。印刷电路板包括在其上形成的工作电极和参考电极。生物传送器可操作地连接到工作电极，并且当经受施加在工作电极和参考电极上的电位时，在特异性识别样品中待检测的靶分子时，能够产生电化学信号。在一些实施例中，印刷电路板生物传感器可以包括用于检测样品中的靶分子的系统的一部分。除了生物传感器之外，这样的系统可以包括当跨越至少一个参考电极和至少一个工作电极施加电势时检测电化学信号的装置和/或用于施加电势的装置。印刷电路板及包括该印刷电路板的系统也可以包括根据本发明使用说明书出售的套件。

44. 发明申请

WO00066779A1 COMPARATIVE FLUORESCENCE HYBRIDIZATION TO OLIGONUCLEOTIDE MICROARRAYS 审中-公开
标题翻译：对寡核苷酸微量比较的比较荧光杂交
公开(公告)号：WO00066779A1
公开(公告)日：2000-11-09
申请号：PCT/US2000/011433
申请日：2000-04-28
IPC分类号： C12Q1/68 , C07H21/02 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/6809 , C12Q1/6855 , C12Q2525/155 , C12Q2525/161 , C12Q2565/519 , C12Q2539/107 , C12Q2537/143
摘要： The present invention provides methods of determining relative copy number of target nucleic acid sequences and precise mapping of chromosomal abnormalities associated with disease. The methods of the invention use target nucleic acid sequences immobilized on a solid surface, to which a sample comprising two sets of differentially labeled nucleic acid sequences are hybridized. The hybridization of the labeled nucleic acid sequences to the solid surface is then detected using standard techniques.
摘要翻译：本发明提供确定靶核酸序列的相对拷贝数和与疾病相关的染色体异常的精确映射的方法。本发明的方法使用固定在固体表面上的靶核酸序列，其中包含两组差异标记的核酸序列的样品与之杂交。然后使用标准技术检测标记的核酸序列与固体表面的杂交。

45. 发明申请

WO0022163A2 METHOD AND KIT FOR DIRECTLY DETECTING NUCLEOTIDE SEQUENCES, AMINO ACID SEQUENCES OR ANTIGENS 审中-公开
标题翻译：用于直接检测核苷酸序列，氨基酸序列或抗原的方法和试剂盒
公开(公告)号：WO0022163A2
公开(公告)日：2000-04-20
申请号：PCT/EP9907709
申请日：1999-10-11
申请人： ATTOMOL GMBH MOLEKULARE DIAGNO , LEHMANN WERNER
发明人： LEHMANN WERNER
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6804 , C12Q1/6853 , C12Q2565/519 , C12Q2521/301 , C12Q2521/101 , C12Q2525/155 , C12Q2525/131 , C12Q2531/119 , C12Q2527/101
摘要： The invention relates to a method called Restrictase Chain Reaction (RCR) for directly detecting target nucleotide or target amino acid sequences or target antigens by amplification of a nucleotide sequence using DNA polymerase, a restriction enzyme and a matrix DNA, on which an initial primer is bonded and amplified. The invention also relates to a kit containing said matrix DNA and the utilization of said kit in medical diagnosis and trace analysis in legal medicine and for analysis related to the environment, food products and medicaments.
摘要翻译：本发明涉及一种如限制性内链式反应由DNA聚合酶，限制性内切酶的核苷酸序列的扩增已知的用于直接检测靶核苷酸或靶氨基酸序列的靶抗原或（RCR）方法，并且在一个矩阵-DNA 通过这些引物的初始引物的结合而被扩增。本发明还涉及包含该基质DNA的试剂盒以及该试剂盒在医学诊断和犯罪痕量分析以及环境，生命和药物分析中的用途。

46. 发明申请

WO98058081A1 METHODS FOR STOOL SAMPLE PREPARATION 审中-公开
标题翻译： STOOL样品制备方法
公开(公告)号：WO98058081A1
公开(公告)日：1998-12-23
申请号：PCT/US1998/011550
申请日：1998-06-04
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q1/6806 , C12Q1/6827 , C12Q2600/156 , C12Q2565/519 , C12Q2527/125 , C12Q2535/131
摘要： The present invention provides methods for the preparation of stool samples to increase the yield of relevant DNA, and further provides methods for isolating and analyzing target DNA for characteristics indicative of colorectal cancer. Methods for screening patients for the presence of cancerous or pre-cancerous colorectal lesions are also provided.
摘要翻译：本发明提供了用于制备粪便样品以增加相关DNA产量的方法，并且还提供了用于分离和分析指示结肠直肠癌特征的靶DNA的方法。还提供了筛选患者癌变或癌前结肠直肠损伤的方法。

47. 发明申请

WO9811253A3 METHOD AND KIT FOR DIAGNOSING AND/OR QUANTIFYING BY SANDWICH HYBRIDISATION OF NUCLEIC ACID SEQUENCES ON SOLID SUPPORT 审中-公开
标题翻译：用于固体支持的核酸序列的三聚体杂交的诊断和/或定量的方法和工具包
公开(公告)号：WO9811253A3
公开(公告)日：1998-05-07
申请号：PCT/BE9700102
申请日：1997-09-09
申请人： REMACLE JOSE , ALEXANDRE ISABELLE , ZAMMATTEO NATHALIE , ERNEST ISABELLE
发明人： REMACLE JOSE , ALEXANDRE ISABELLE , ZAMMATTEO NATHALIE , ERNEST ISABELLE
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6816 , C12Q1/6837 , C12Q2565/519 , C12Q2545/114 , C12Q2537/125
摘要： The invention concerns a method for detecting and/or quantifying a target nucleotide sequence (2) present in a biological sample characterised in that it comprises a 'sandwich' type contacting of the target nucleotide sequence (2) with a trapper single strand nucleotide sequence (5) fixed on an insoluble solid support (3) and complementary of part (7) of said target nucleotide sequence (2) and with one or several nucleotide sequences (6, 11) of which at least one is marked (6), the said nucleotide sequence(s) (6, 11) being complementary of another part (8) of the target nucleotide sequence (2); in that the trapper nucleotide sequence (5) is fixed covalently by one of its ends on the solid support (3), and has a length between 50 and 500 bases; and in that the part (10) of the trapper nucleotide sequence (5) which is not hybridised with the part (7) of the target nucleotide sequence (2) has less than 60 bases. The invention also concerns the kit for detecting and/or quantifying for implementing this method and the use of one standard quantifying nucleotide sequence.

48. 发明申请

WO1996023903A1 METHOD FOR DETECTING NUCLEIC ACID SEQUENCE VARIATIONS 审中-公开
标题翻译：检测核酸序列变异的方法
公开(公告)号：WO1996023903A1
公开(公告)日：1996-08-08
申请号：PCT/SE1996000095
申请日：1996-01-30
申请人： LANDEGREN, Ulf , LAGERQVIST, Arild
IPC分类号： C12Q01/68
CPC分类号： C12Q1/683 , C12Q1/6827 , C12Q1/6837 , C12Q2565/519 , C12Q2537/113 , C12Q2535/125
摘要： A method of analyzing nucleic acid-containing samples for sequence variations relative to standard nucleic acid sequences, which method comprises providing a single-stranded standard nucleic acid sequence immobilized on a solid support, hybridizing a nucleic acid strand derived from a sample to the immobilized strand, subjecting the nucleic acid complex formed to (i) mismatch-induced cleavage or (ii) mismatch-terminated extension reactions, and detecting possible cleavage or extension-termination by optical measurement on the solid support.
摘要翻译：一种分析含有核酸的样品相对于标准核酸序列的序列变异的方法，该方法包括提供固定在固体支持物上的单链标准核酸序列，将衍生自样品的核酸链与固定链杂交使形成的核酸复合物（i）错配诱导的切割或（ii）错配终止的延伸反应，并通过在固体支持物上的光学测量来检测可能的切割或延伸终止。

49. 发明申请

WO1993017126A1 NOVEL OLIGONUCLEOTIDE ARRAYS AND THEIR USE FOR SORTING, ISOLATING, SEQUENCING, AND MANIPULATING NUCLEIC ACIDS 审中-公开
标题翻译：新型寡核苷酸阵列及其用于分选，分离，测序和操纵核酸的用途
公开(公告)号：WO1993017126A1
公开(公告)日：1993-09-02
申请号：PCT/US1993001552
申请日：1993-02-19
申请人： THE PUBLIC HEALTH RESEARCH INSTITUTE OF THE CITY ...
发明人： THE PUBLIC HEALTH RESEARCH INSTITUTE OF THE CITY ... , CHETVERIN, Alexander, B. , KRAMER, Fred, Russell
IPC分类号： C12Q01/68
CPC分类号： B82Y30/00 , B01J19/0046 , B01J2219/00313 , B01J2219/00315 , B01J2219/00527 , B01J2219/00529 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00621 , B01J2219/00626 , B01J2219/00637 , B01J2219/00644 , B01J2219/00659 , B01J2219/00662 , B01J2219/00675 , B01J2219/00722 , C12N15/10 , C12Q1/6806 , C12Q1/6811 , C12Q1/6834 , C12Q1/6837 , C12Q1/6853 , C12Q1/686 , C12Q1/6874 , C40B40/06 , C40B50/14 , C40B60/14 , Y10S435/81 , Y10S436/808 , C12Q2563/179 , C12Q2531/107 , C12Q2525/131 , C12Q2525/191 , C12Q2565/519 , C12Q2565/537 , C12Q2525/179
摘要： The present invention relates to new oligonucleotide arrays and methods of using oligonucleotide arrays. Binary oligonucleotide arrays, having binary oligonucleotides characterized by a constant nucleotide sequence adjacent to a variable nucleotide sequence, are used for sorting and surveying nucleic acid strands. Oligonucleotide arrays are used for sorting mixtures of nucleic acid strands, making immobilized partial copies of nucleic strands, ligating strands, or introducing site directed mutations into strands. Information is obtained for determining the sequence of a nucleic acid strand, alone or in a mixture, by generating partials of the strand and, for groups of partials having the same terminal variable oligonucleotide, separately determining the presence and sequence of all variable oligonucleotides. Arrays are also used to order previously sequenced nucleic acid fragments and to allocate ordered allelic fragments to chromosomal linkage groups.
摘要翻译：本发明涉及新的寡核苷酸阵列和使用寡核苷酸阵列的方法。具有特征在于与可变核苷酸序列相邻的恒定核苷酸序列的二元寡核苷酸的二元寡核苷酸阵列用于分选和测量核酸链。寡核苷酸阵列用于分选核酸链的混合物，使核链的固定化部分拷贝，连接链或引入位点定向的突变进入链。通过产生部分链，以及对于具有相同末端可变寡核苷酸的部分组，分别确定所有可变寡核苷酸的存在和序列，获得单独或混合物中核酸链的序列的信息。阵列也用于对先前测序的核酸片段进行排序并将有序等位基因片段分配给染色体连锁基团。

50. 发明申请

WO1990011373A1 PROCESS FOR RAPID NUCLEIC ACID DETECTION 审中-公开
标题翻译：快速检测核酸的方法
公开(公告)号：WO1990011373A1
公开(公告)日：1990-10-04
申请号：PCT/US1990001533
申请日：1990-03-26
申请人： E.I. DU PONT DE NEMOURS AND COMPANY
发明人： E.I. DU PONT DE NEMOURS AND COMPANY , HOBBS, Frank, Worden, Jr. , LITT, Gerald, Joseph , MILLER, Jeffrey, Allan
IPC分类号： C12Q01/68
CPC分类号： C12Q1/703 , C12Q1/686 , C12Q2565/519 , C12Q2563/131 , C12Q2563/107
摘要： A nucleic acid probe assay which incorporates a deoxyribonucleotide triphosphate containing a reporter moiety into a nucleic acid amplification process followed by affinity capture and detection of the reporter moiety is provided.
摘要翻译：提供了一种将含有报道部分的脱氧核糖核苷酸三磷酸结合到核酸扩增过程中，然后亲和捕获和检测报道部分的核酸探针测定。

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