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    • 44. 发明申请
    • CHIMERIC HUMAN SWEET-UMAMI AND UMAMI-SWEET TASTE RECEPTORS
    • CHIMERIC人类SWEET-UMAMI和UMAMI-SWEET TASTE RECEPTORS
    • WO2007047988A2
    • 2007-04-26
    • PCT/US2006/041158
    • 2006-10-19
    • SENOMYX, INC.LI, XiaodongZHANG, FengXU, HongLI, Qing
    • LI, XiaodongZHANG, FengXU, HongLI, Qing
    • C07K14/705
    • C07K14/705C12N2503/00C12N2510/00
    • This invention relates to chimeric taste receptors comprising the extracellular portion of one TlR or a variant or fragment thereof, either TlRl or T1R2, and the transmembrane portion of another TlR or a variant or fragment thereof, either TlRl or T1R2, preferably associated with a T1R3 polypeptide and a suitable G protein. These chimeric taste receptors and cells which express such chimeric taste receptors are useful in assays for identifying sweet and umami ligands as well in assays for identifying sweet and umami enhancers. Additionally, these chimeric taste receptors and cells which express same can be used to map and determine where specific sweet and umami ligands interact with their respective receptors and to elucidate the mechanism of receptor activation.
    • 本发明涉及包含一种TlR的细胞外部分或其变体或片段,即T1R1或T1R2的嵌合味觉感受器,以及T1R1或其变体或片段的跨膜部分,T1R1或T1R2,优选与T1R3相关 多肽和合适的G蛋白。 这些嵌合味觉受体和表达这种嵌合味觉受体的细胞可用于鉴定甜味和鲜味配体的测定以及用于鉴定甜味和鲜味增强剂的测定中。 此外,这些嵌合味觉受体和表达相同的细胞可以用于绘制和确定特定甜味和鲜味配体与其各自受体相互作用的位置,并阐明受体活化的机制。