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    • 37. 发明申请
    • PURIFICATION OF THE LEADING FRONT MIGRATORY CELLS
    • 提纯前导迁移细胞
    • WO2003068931A2
    • 2003-08-21
    • PCT/US2003/004453
    • 2003-02-12
    • THE SCRIPPS RESEARCH INSTITUTEKLEMKE, Richard, L.
    • KLEMKE, Richard, L.
    • C12N
    • G01N33/5076A61K31/739G01N33/567
    • The invention relates generally to methods of modulating cell migration. Included in the methods of the invention are methods of identifying the state of a pseudopodium in cell migration and methods of inducing extension or retraction of a pseudopodia from a cell. The invention also relates to methods of screening for and identifying an agent effective in inducing extension or retraction of a pseudopodium and therefore affecting cell migration. Agents that can modulate cell migration are useful in treatment of conditions in which cell migration plays a role. Such conditions can include wound healing, angiogenesis, and metastasis of a disease from one location to another. Additionally, the invention provides methods of biochemically separating the pseudopodium of a cell from the remainder of the cell body and methods of determining the proteins present in the pseudopodium and cell body. The invention also includes a pseudopodium isolated by the methods of the invention.
    • 本发明一般涉及调节细胞迁移的方法。 本发明的方法中包括鉴定细胞迁移中伪足的状态的方法和诱导伪足从细胞延伸或缩回的方法。 本发明还涉及筛选和鉴定有效诱导伪足的延伸或回缩并因此影响细胞迁移的试剂的方法。 可以调节细胞迁移的药剂可用于治疗细胞迁移起作用的病症。 这样的病症可以包括伤口愈合,血管生成和疾病从一个位置到另一个位置的转移。 另外,本发明提供了从细胞体的其余部分生物化学分离细胞伪足的方法以及确定存在于伪足和细胞体中的蛋白质的方法。 本发明还包括通过本发明的方法分离的伪足。