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31. 发明申请

WO2007027328A2 MICROBIAL SULFOXIDATION AND AMIDATION OF BENZHDRYLSULFANYL CARBOXYLIC ACIDS AND USES THEREOF 审中-公开
标题翻译：苯甲基磺酰基羧酸的微生物硫化和酰胺化及其应用
公开(公告)号：WO2007027328A2
公开(公告)日：2007-03-08
申请号：PCT/US2006/029098
申请日：2006-07-27
申请人： UNIVERSITY OF IOWA RESEARCH FOUNDATION , OLIVO, Horacio, F. , OSORIO-LOZADA, Antonio, Victor
发明人： OLIVO, Horacio, F. , OSORIO-LOZADA, Antonio, Victor
CPC分类号： C12P11/00 , C12P13/02 , C12P41/006
摘要： The present invention provides novel methods for the synthesis of racemic and enantiomers of modafinil via microbial oxidation-amidation transformation. The methods include the successive oxidation-amidation of benzhydrylsulfanyl carboxylic acid to produce racemic and enantiomers of modafinil using at least one microorganism of yeast, bacteria, or fungus. Also disclosed are pharmaceutical compositions of racemic and enantiomers of modafinil along with their use in the treatment of diseases, including attention deficit hyperactivity disorder and drug addiction.
摘要翻译：本发明提供了通过微生物氧化 - 酰胺化转化来合成莫达非尼的外消旋和对映体的新方法。该方法包括使用至少一种酵母，细菌或真菌的微生物对二苯甲基硫烷基羧酸进行连续氧化 - 酰胺化以产生莫达非尼的外消旋和对映异构体。还公开了莫达非尼的外消旋和对映异构体的药物组合物以及它们在治疗疾病（包括注意力缺陷多动症和药物成瘾）中的用途。

32. 发明申请

WO2003042412A1 RECA-NEGATIVE AND RHAB-NEGATIVE MICROORGANISM 审中-公开
标题翻译： RECA阴性和RHAB阴性微生物
公开(公告)号：WO2003042412A1
公开(公告)日：2003-05-22
申请号：PCT/EP2002/011979
申请日：2002-11-11
申请人： DEGUSSA AG , MAY, Oliver , LIEBETON, Klaus , ECK, Jürgen
发明人： MAY, Oliver , LIEBETON, Klaus , ECK, Jürgen
IPC分类号： C12R1/19
CPC分类号： C12R1/19 , C12N15/68 , C12N15/70 , C12P13/04 , C12P21/02 , C12P41/006
摘要： The present invention describes new microorganisms specifically for use as expression systems for rhamnose-inducible promoters. Due to the combination of rhamnose and recombination deficiency artificially created in these organisms, expression systems which require less of the expensive rhamnose for induction and therefore can be induced more inexpensively, and have a higher genetic stability are obtained.
摘要翻译：本发明描述了专门用作鼠李糖诱导型启动子表达系统的新微生物。由于在这些生物体中人造产生的鼠李糖和重组缺陷的组合，需要较少昂贵的鼠李糖用于诱导并因此可以更廉价地诱导并具有较高遗传稳定性的表达系统。

33. 发明申请

WO2003010183A2 PROCESS FOR PREPARING VARIANT POLYNUCLEOTIDES 审中-公开
标题翻译：制备变体多核苷酸的方法
公开(公告)号：WO2003010183A2
公开(公告)日：2003-02-06
申请号：PCT/EP2002/008225
申请日：2002-07-23
申请人： DSM N.V. , BOVENBERG, Roelof, Ary, Lans , KERKMAN, Richard
发明人： BOVENBERG, Roelof, Ary, Lans , KERKMAN, Richard
IPC分类号： C07K
CPC分类号： C12N9/80 , C12N15/102 , C12N15/1027 , C12N15/1031 , C12P13/04 , C12P41/006
摘要： The present invention discloses a process for the preparation of variant polynucleotides using a combination of mutagenesis of a starting population of polynucleotides and recombination of the mutated polynucleotides. The process comprises the steps of subjecting a population of polynucleotides to (a series of) two (or more) separate PCR's comprising a first PCR with a forward mutation-specific primer for a position to be mutated and a reverse universal primer and a second PCR with a forward universal primer and a reverse mutation-specific primer for a position to be mutated. The products of the (series of) two (or more) PCR's are assembled by a polymerase, preferably in one tube.
摘要翻译：本发明公开了使用多核苷酸的起始群体的诱变和突变的多核苷酸的重组的组合制备变体多核苷酸的方法。该方法包括以下步骤：使多核苷酸群体经受包含第一PCR的（一系列）两个（或更多个）单独PCR，其中突变位置的正向突变特异性引物和反向通用引物以及第二PCR 用正向通用引物和用于突变位置的回复突变特异性引物。（一系列）两种（或多种）PCR的产物通过聚合酶组装，优选在一个管中。

34. 发明申请

WO02020820A1 METHOD FOR THE PREPARATION OF ENANTIOMERICALLY ENRICHED AMINES 审中-公开
标题翻译：制备高效液相色谱的方法
公开(公告)号：WO02020820A1
公开(公告)日：2002-03-14
申请号：PCT/NL2001/000642
申请日：2001-08-30
IPC分类号： C07C237/20 , C12P13/00 , C12P13/02 , C12P13/04 , C12P41/00 , C07C237/06
CPC分类号： C12P13/002 , C12P13/008 , C12P13/02 , C12P13/04 , C12P41/006 , C12P41/007
摘要： Method for the preparation of an enantiomerically enriched acylated amine wherein a phenylacetic acid derivative is brought into contact with a mixture of enantiomers of an amine H2NCR1R2R3, in which R1, R2, R3 are not equal to each other and each independently stand for H, CN, a whether or not substituted (cyclo)alkyl, ary-, alkylaryl or arylalkyl group, whether or not cyclic heteroalkyl or heteroaryl group with one or more N, O or S atoms or in which R1 and R2 (and R3) together with the C-atom to which they have been bound form a (bi)cyclic group that optionally contains one or more N, O of S atoms, in the presence of a Pen-G acylase derived from Alcaligenes faecalis . And method for the preparation of enantiomerically enriched amine of formula H2NCR1R2R3, in which R1, R2, R3 are as defined above, wherein an acylated amine is brought into contact with a Pen-G acylase originating from Alcaligenes faecalis . Preferably enantiomerically enriched phenylglycine amide or an ester of p-hydroxyphenylglycine is applied as phenylacetic acid derivative.
摘要翻译：制备对映异构体富集的酰化胺的方法，其中苯乙酸衍生物与胺H 2 NCR 1 R 2 R 3的对映异构体的混合物接触，其中R 1，R 2，R 3不相同，并且各自独立代表H，CN 无论是否具有一个或多个N，O或S原子的环状杂烷基或杂芳基，或其中R 1和R 2（和R 3）与该取代的（环）烷基，芳基 - 烷基芳基或芳烷基它们已经被结合的C原子形成任选地含有一个或多个S原子的N，O的（双）环基团，在来源于产碱杆菌的Pen-G酰基转移酶存在下。以及制备式H2NCR1R2R3的对映异构体富集的胺的方法，其中R 1，R 2，R 3如上定义，其中酰化胺与源自产碱杆菌的Pen-G酰基转移酶接触。优选对映体富集的苯基甘氨酸酰胺或对羟基苯基甘氨酸的酯作为苯乙酸衍生物。

35. 发明申请

WO9937754A3 ENZYME ACTIVATED SUPPORTS FOR ENANTIOMERIC SEPARATIONS 审中-公开
标题翻译： ENZYME激活的支持用于宽带分离
公开(公告)号：WO9937754A3
公开(公告)日：1999-10-14
申请号：PCT/US9901645
申请日：1999-01-26
申请人： PHARM ECO LAB INC , GABRIEL RICHARD L , SWANSON DAVID A
发明人： GABRIEL RICHARD L , SWANSON DAVID A
IPC分类号： C07D411/04 , C12N9/18 , C12N9/36 , C12N11/00 , C12N11/08 , C12P41/00
CPC分类号： C12N9/2462 , C12N9/18 , C12N11/00 , C12N11/08 , C12P41/003 , C12P41/004 , C12P41/006
摘要： An enzyme is immobilized on a solid support and can react selectively with one enantiomer in an enantiomeric mixture. A methods of using the enzyme immobilized solid support in conjunct with a separating means to separate enantiomeric mixtures is described. An apparatus for separating an enantiomeric mixture using an enzyme immobilized on a solid support is also described.
摘要翻译：酶固定在固体支持物上，并且可以与对映体混合物中的一种对映异构体选择性反应。描述了使用酶固定的固体支持物与分离装置结合以分离对映体混合物的方法。还描述了使用固定在固体支持物上的酶分离对映体混合物的装置。

36. 发明申请

WO98048040A1 PROCESS FOR PRODUCING L-ALLYSINE ACETALS 审中-公开
标题翻译：生产L-亚氨酸乙酯的方法
公开(公告)号：WO98048040A1
公开(公告)日：1998-10-29
申请号：PCT/JP1998/001830
申请日：1998-04-22
IPC分类号： C12P7/40 , C12P7/42 , C12P13/04 , C12P41/00
CPC分类号： C12P7/40 , C12P13/04 , C12P41/006
摘要： A process for efficiently producing L-allysine acetals in short steps, which comprises reacting a D,L-allysine acetal of general formula: (1) (wherein R and R are the same or different from each other and each represents an alkyl group of 1 to 8 carbon atoms, or R and R together form a ring to represent an alkylene group having 2 to 8 carbon atoms) in the presence of an enzyme having the activity for sterically selectively and oxidatively deaminating D-amino acids to convert the D,L-allysine acetal into a mixture of a 2-oxo-6,6-dialkoxyhexanoic acid of general formula (2) (wherein R and R are each as defined above) with an L-allysine acetal of general formula (3) (wherein R and R are each as defined above) and then recovering the L-allysine acetal.
摘要翻译：一种用于在短时间内有效生产L-烯丙基乙缩醛的方法，其包括使通式为（1）（其中R 1和R 2彼此相同或不同）的D，L-烯丙基乙缩醛反应，以及各自表示1至8个碳原子的烷基，或者R 1和R 2一起形成环，以表示具有2至8个碳原子的亚烷基）在具有空间选择性活性的酶存在下和氧化脱氨基的D-氨基酸以将D，L-烯丙基乙缩醛转化成通式（2）的2-氧代-6,6-二烷氧基己酸（其中R 1和R 2各自为如上所述）与通式（3）的L-烯丙基乙缩醛（其中R 1和R 2各自如上所定义），然后回收L-赖氨酸缩醛。

37. 发明申请

WO1998010075A1 MICROORGANISM, LACTAMASE ENZYME OBTAINED THEREFROM, AND THEIR USE 审中-公开
标题翻译： MICROORGANISM，LACTAMASE ENZYME获得者及其使用
公开(公告)号：WO1998010075A1
公开(公告)日：1998-03-12
申请号：PCT/GB1997002344
申请日：1997-09-01
申请人： CHIROSCIENCE LIMITED
发明人： CHIROSCIENCE LIMITED , WISDOM, Richard, Anthony , LEE, Caroline, Susan , BROWN, Robert, Christopher
IPC分类号： C12N15/55
CPC分类号： C12N9/86 , C12P41/006
摘要： A lactamase enzyme having good stability, capable of hydrolysing an enantiomer of the bicyclic lactam, 2-azabicyclo[2.2.1]hept-5-en-3-one, to give (-) lactam and (+) amino-acid, has been found in a strain of Comamonas acidivorans. The enzyme has been isolated and cloned, and its structure identified.
摘要翻译：能够水解双环内酰胺2-氮杂双环[2.2.1]庚-5-烯-3-酮的对映异构体以得到（ - ）内酰胺和（+）氨基酸的具有良好稳定性的内酰胺酶具有被发现在一个Comamonas acidivorans菌株中。酶已被分离并克隆，其结构鉴定。

38. 发明申请

WO9801568A3 METHOD OF PREPARING (S) - OR (R) -3,3,3-TRIFLUORO-2-HYDROXY-2- METHYLPROPIONIC ACID 审中-公开
标题翻译：制备（S） - （（R）-3,3,3-三氟-2-羟基-2-甲基丙酸的方法
公开(公告)号：WO9801568A3
公开(公告)日：1998-02-19
申请号：PCT/EP9703670
申请日：1997-07-10
申请人： LONZA AG , BRIEDEN WALTER , NAUGHTON ANDREW , ROBINS KAREN , SHAW NICHOLAS , TINSCHERT ANDREAS , ZIMMERMANN THOMAS
发明人： BRIEDEN WALTER , NAUGHTON ANDREW , ROBINS KAREN , SHAW NICHOLAS , TINSCHERT ANDREAS , ZIMMERMANN THOMAS
IPC分类号： C12N15/09 , C07C235/06 , C12N1/21 , C12N9/80 , C12N15/55 , C12P13/02 , C12P41/00 , C12R1/06 , C07C231/06 , C12N1/20 , C12P7/42
CPC分类号： C12N1/20 , C07C235/06 , C12N9/80 , C12P7/42 , C12P13/02 , C12P41/006
摘要： Described are new micro-organisms and a new enzyme capable of using as sole source of nitrogen the propionic acid amide of formula (VI), in racemate form or as optically active isomers. Described also is a method of preparing (S) - or (R)-3,3,3-trifluoro-2-hydroxy-2-methylpropionic acid of formulas (I) and (II) starting from trifluoroaceto-acetic ester. The first three process steps are chemical, the fourth process step microbiological.

39. 发明申请

WO1994006930A1 A PROCESS FOR THE PREPARATION OF ENANTIOMERIC 2-ALKANOIC ACIDS 审中-公开
标题翻译：一种制备高效2-羟基酸的方法
公开(公告)号：WO1994006930A1
公开(公告)日：1994-03-31
申请号：PCT/US1993006821
申请日：1993-07-23
申请人： E.I. DU PONT DE NEMUORS AND COMPANY , FALLON, Robert, Donald , STIEGLITZ, Barry
发明人： E.I. DU PONT DE NEMUORS AND COMPANY
IPC分类号： C12P41/00
CPC分类号： C12P41/006 , C12P7/40 , C12P13/02
摘要： Converting 2-alkyl nitriles of formula (1), wherein A and R are as defined in the text, to the corresponding 2-alkanoic acids; 2-alkyl nitriles to the corresponding amides; and 2-alkyl amides to the corresponding acids, all employing one or both of Pseudomonas putida 5B-MGN-2p and Pseudomonas chlororaphis B23.
摘要翻译：将式（1）的2-烷基腈（其中A和R 1如本文定义）转化为相应的2-链烷酸; 2-烷基腈与相应的酰胺; 和2-烷基酰胺与所有相应的酸，都使用恶臭假单胞菌5B-MGN-2p和绿假单胞菌B23中的一种或两种。

40. 发明申请

WO1992018477A1 AZABICYCLOHEPTANONE 审中-公开
公开(公告)号：WO1992018477A1
公开(公告)日：1992-10-29
申请号：PCT/GB1992000731
申请日：1992-04-21
申请人： CHIROS LIMITED , EVANS, Christopher, Thomas , ROBERTS, Stanley, Michael , SUTHERLAND, Alan, Gordon
发明人： CHIROS LIMITED
IPC分类号： C07D205/12
CPC分类号： C07C229/48 , C07D205/12 , C12P13/04 , C12P41/006
摘要： The individual enantiomers of the bicyclic beta -lactam compounds (Ia), (Ib), (IIa) or (IIb), optionally substituted by non-interfering substituent(s). The novel enantiomers can be obtained by biotransformation. The Ia or IIa compounds can be used for the synthesis of chiral cis-pentacin.
摘要翻译：双环β-内酰胺化合物（Ia），（Ib），（IIa）或（IIb）的单独对映异构体，任选被非干扰性取代基取代。新型对映异构体可以通过生物转化获得。 Ia或IIa化合物可用于合成手性顺式五肽。

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