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31. 发明申请

WO2016186953A1 GUIDE RNA/CAS ENDONUCLEASE SYSTEMS 审中-公开
标题翻译：指南RNA / CAS内切酶体系
公开(公告)号：WO2016186953A1
公开(公告)日：2016-11-24
申请号：PCT/US2016/032073
申请日：2016-05-12
申请人： PIONEER HI BRED INTERNATIONAL INC
发明人： CIGAN, Andrew Mark , GASIUNAS, Giedrius , KARVELIS, Tautvydas , SIKSNYS, Virginijus , YOUNG, Joshua K.
IPC分类号： C12N9/22 , C12N15/113 , C12N15/90
CPC分类号： C12N15/1093 , C12N9/22 , C12N15/102 , C12N15/1051 , C12N15/113 , C12N15/8213 , C12N15/902 , C12N2310/20 , C12N2320/10 , C12Q1/6811 , C12Q2521/301 , C12Q2525/179 , C12Q2525/191 , C12Q2535/122
摘要： Compositions and methods are provided for novel guide RNA/ Cas endonuclease systems. Type II Cas9 endonuclease systems originating from Brevibacillus laterosporus, Lactobacillus reuteri Mlc3, Lactobacillus rossiae DSM 15814, Pediococcus pentosaceus SL4, Lactobacillus nodensis JCM 14932, Sulfurospirillum sp . SCADC, Bifidobacterium thermophilum DSM 20210, Loktanella vestfoldensis, Sphingomonas sanxanigenens NX02, Epilithonimonas tenax DSM 16811, Sporocytophaga myxococcoides are described herein. The present disclosure also describes methods for genome modification of a target sequence in the genome of a cell, for gene editing, and for inserting a polynucleotide of interest into the genome of a cell.
摘要翻译：提供了新的RNA / Cas内切核酸酶系统的组成和方法。 II型Cas9内切核酸酶系统来源于短孢杆菌（Brevibacillus soosporus），罗伊氏乳杆菌（Luterobacillus reuteri）Mlc3，罗氏假丝酵母（DSM）15814，戊糖片球菌SL4，乳酸乳杆菌JCM 14932， SCADC，双歧杆菌嗜热乳杆菌DSM 20210，Loktanella vestfoldensis，Sphingomonas sanxanigenens NX02，Epilithonimonas tenax DSM 16811，孢子体孢子虫粘球菌在本文中描述。本公开还描述了用于基因组修饰细胞基因组中的靶序列，用于基因编辑以及将感兴趣的多核苷酸插入细胞基因组的方法。

32. 发明申请

WO2016183005A1 METHODS AND COMPOSITIONS TO ALTER FLOWERING TIME IN SORGHUM 审中-公开
标题翻译：方法和组合物，以提高萌发时间
公开(公告)号：WO2016183005A1
公开(公告)日：2016-11-17
申请号：PCT/US2016/031462
申请日：2016-05-09
申请人： E I DU PONT DE NEMOURS AND COMPANY , NEXSTEPPE INC , PIONEER HI BRED INTERNATIONAL INC
发明人： JUNG, Mark Timothy , FRANKS, Cleve Douglas , MAYOR, Maria Laura , OREAR, Jerry D. , PORTER, Kay , SCHNEEBERGER, Richard
IPC分类号： A01H1/04 , A01H5/10 , C12Q1/68
CPC分类号： A01H1/04 , A01H5/10 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156
摘要： Compositions and methods useful in identifying and selecting sorghum plants with different flowering times are provided herein. The methods and compositions provided herein can be used to select sorghum plants with modulated photoperiod sensitivity. The methods use molecular genetic markers to identify and/or select sorghum plants with different flowering times and or/to identify and select sorghum plants with different photoperiod sensitivities.
摘要翻译：本文提供了用于鉴定和选择具有不同开花时间的高粱植物的组合物和方法。本文提供的方法和组合物可用于选择具有调节光周期灵敏度的高粱植物。该方法使用分子遗传标记来鉴定和/或选择具有不同开花时间的高粱植物和/或识别和选择具有不同光周期敏感性的高粱植物。

33. 发明申请

WO2016138106A1 COMPOSITIONS AND METHODS TO CONTROL INSECT PESTS 审中-公开
标题翻译：组合物及其控制方法
公开(公告)号：WO2016138106A1
公开(公告)日：2016-09-01
申请号：PCT/US2016/019313
申请日：2016-02-24
申请人： PIONEER HI BRED INTERNATIONAL INC , E I DU PONT DE NEMOURS AND COMPANY
发明人： HU, Xu , NIU, Xiping , PRESNAIL, James Kevin , RICHTMAN, Nina , ZHAO, Joe
IPC分类号： C12N15/11 , C12N15/82 , A01N63/00
CPC分类号： C12N15/113 , A01N63/02 , C12N15/111 , C12N15/8286 , C12N2310/14 , Y02A40/162
摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a plant insect pest, such as a Coleopteran plant pest or a Diabrotica plant pest, decrease the expression of a target sequence in the pest. Disclosed are various target polynucleotides set forth in any one of SEQ ID NOS: 1-54 and 81-84 disclosed herein, or variants and fragments thereof, or complements thereof, wherein a decrease in expression of one or more of the sequences in the target pest controls the pest (i.e., has insecticidal activity). Plants, plant parts, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
摘要翻译：提供了使用沉积元件的方法和组合物，当由植物昆虫害虫（例如鞘翅目植物害虫或双壳花属植物害虫）摄入时，降低害虫中靶序列的表达。公开了本文公开的SEQ ID NO：1-54和81-84中任一个所示的各种靶多核苷酸，或其变体和片段，或其互补序列，其中靶中的一个或多个序列的表达降低害虫控制害虫（即具有杀虫活性）。还提供了包含本发明的沉默元件或其活性变体或片段的植物，植物部分，细菌和其它宿主细胞。

34. 发明申请

WO2016061206A1 INSECTICIDAL PROTEINS AND METHODS FOR THEIR USE 审中-公开
标题翻译：杀虫蛋白及其使用方法
公开(公告)号：WO2016061206A1
公开(公告)日：2016-04-21
申请号：PCT/US2015/055502
申请日：2015-10-14
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E. I. DU PONT DE NEMOURS AND COMPANY
发明人： BARRY, Jennifer K. , CLARK, Deborah W. , ENGLISH, James , ONG, Azalea , SCHEPERS, Eric , QI, Julie , RICE, Janet
IPC分类号： C12N15/82 , C07K14/415 , A01N65/00
CPC分类号： C07K14/415 , A01N37/46 , A01N47/44 , A01N65/00 , A01N65/04 , C07K2319/00 , C12N15/8286 , Y02A40/162
摘要： Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.
摘要翻译：提供了控制害虫的组成和方法。该方法涉及用编码杀虫蛋白的核酸序列转化生物体。特别地，核酸序列可用于制备具有杀虫活性的植物和微生物。因此，提供了转化的细菌，植物，植物细胞，植物组织和种子。组合物是杀虫的核酸和细菌物种的蛋白质。这些序列用于构建表达载体，用于随后转化为感兴趣的生物体，包括植物，作为分离其他同源（或部分同源的）基因的探针。杀虫蛋白用于控制，抑制生长或杀死鳞翅目，鞘翅目，双翅目，真菌，半翅目和线虫害虫种群并生产具有杀虫活性的组合物。

35. 发明申请

WO2016060949A1 INSECTICIDAL POLYPEPTIDES HAVING BROAD SPECTRUM ACTIVITY AND USES THEREOF 审中-公开
标题翻译：具有广谱活性的杀虫多肽及其用途
公开(公告)号：WO2016060949A1
公开(公告)日：2016-04-21
申请号：PCT/US2015/054869
申请日：2015-10-09
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： ABAD, Andre R. , CROW, Andrew C. , POLAND, Brad , SHI, Xiaomei , WOLFE, Thomas C.
IPC分类号： C12N5/14
CPC分类号： C12N15/8286 , A01N63/02 , C07K14/32 , C12N5/04 , C12N15/8279 , C12N2510/02 , C12P21/02
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera and Coleoptera . Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.
摘要翻译：本发明提供了从编码具有抗虫害杀虫活性的多肽的苏云金芽孢杆菌菌株获得的核酸及其变体和片段，所述多肽对包括鳞翅目昆虫 >和<鞘翅目> 。本发明的具体实施方案提供了编码杀虫蛋白的分离的核酸，杀虫组合物，DNA构建体和包含实施方案的核酸的转化的微生物和植物。这些组合物可用于防治害虫，特别是植物害虫的方法。

36. 发明申请

WO2016040876A1 METHODS AND COMPOSITIONS FOR PLANT PEST CONTROL 审中-公开
标题翻译：植物病虫害防治方法与组合
公开(公告)号：WO2016040876A1
公开(公告)日：2016-03-17
申请号：PCT/US2015/049806
申请日：2015-09-11
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , IOWA STATE UNIVERSITY RESEARCH FOUNDATION, INC. , NORTH CAROLINA STATE UNIVERSITY , UNIVERSITY OF GEORGIA RESEARCH FOUNDATION, INC. , THE CURATORS OF THE UNIVERSITY OF MISSOURI
发明人： WEI, Jun-zhi , WU, Gusui , SIMMONS, Carl R. , BAUM, Thomas , DAVIS, Eric Lee , HUSSEY, Richard , MITCHUM, Melissa G.
IPC分类号： C12N15/82 , C12N15/13 , C07K14/435
CPC分类号： C12N15/8285 , C07K14/4354 , Y02A40/164
摘要： The present invention comprises methods and compositions for controlling nematode parasitism in host plant. The present invention comprises novel polynucleotides and polypeptides encoded by such polynucleotides comprising one or more nucleic acid sequences disclosed herein having a nucleotide sequence comprising any one of SEQ ID NOs: 162-179, a fragment or variant thereof, or a complement or partial complement or substantial complement thereof, or a polypeptide sequence encoded by SEQ ID NOs: 162-179, or a polypeptide sequence that is a fragment or variant of a polypeptide sequence encoded SEQ ID NOs: 162-179.
摘要翻译：本发明包括用于控制宿主植物中线虫寄生的方法和组合物。本发明包括新的多核苷酸和由这样的多核苷酸编码的多肽，所述多核苷酸包含本文所公开的一种或多种核酸序列，其具有包含SEQ ID NO：162-179中任一项的核苷酸序列，其片段或变体，或补体或部分补体或或其由SEQ ID NO：162-179编码的多肽序列，或作为编码SEQ ID NO：162-179的多肽序列的片段或变体的多肽序列。

37. 发明申请

WO2016040030A1 GENERATION OF SITE-SPECIFIC-INTEGRATION SITES FOR COMPLEX TRAIT LOCI IN CORN AND SOYBEAN, AND METHODS OF USE 审中-公开
标题翻译：玉米和大豆中复合物种位点特异性整合位点的生成及其使用方法
公开(公告)号：WO2016040030A1
公开(公告)日：2016-03-17
申请号：PCT/US2015/047706
申请日：2015-08-31
申请人： E. I. DU PONT DE NEMOURS AND COMPANY , PIONEER HI-BRED INTERNATIONAL, INC.
发明人： CIGAN, Andrew Mark , GAO, Huirong , LIU, Zhan-Bin , MUTTI, Jasdeep , PODLICH, Dean , SCELONGE, Christopher
IPC分类号： C12N15/82 , A01H5/10
CPC分类号： C12N15/8213 , C12N15/8201 , C12N15/8241
摘要： Compositions and methods are provided for introducing transgenic target sites for Site Specific Integration (SSI) and/or polynucleotides of interest into at least one double-strand break target site of a double-strand-break inducing agent in a genomic window of a plant genome. Also provided are methods and compositions for producing a complex trait locus in a genomic window of a plant comprising at least one transgenic target site for site specific integration integrated in at least double-strand-break target site. The double-strand-break target site can be, but is not limited to, a target site for a zinc finger endonuclease, an engineered endonuclease, a meganuclease, a TALENs and/or a Cas endonuclease. The genomic window of said plant can comprise at least one genomic locus of interest such as a trait cassette, a transgene, a mutated gene, a native gene, an edited gene or a site-specific integration (SSI) target site.
摘要翻译：提供组合物和方法用于将位点特异性整合（SSI）和/或目标多核苷酸的转基因靶位点引入植物基因组的基因组窗口中双链断裂诱导剂的至少一个双链断裂靶位点。还提供了用于在植物的基因组窗口中产生复合特征基因座的方法和组合物，其包含至少一个整合在至少双链断裂靶位点中的位点特异性整合的转基因靶位点。双链断裂靶位点可以是但不限于锌指内切核酸酶，工程内切核酸酶，大范围核酸酶，TALENs和/或Cas内切核酸酶的靶位点。所述植物的基因组窗可以包含至少一个感兴趣的基因座，例如性状盒，转基因，突变基因，天然基因，编辑基因或位点特异性整合（SSI）靶位点。

38. 发明申请

WO2016022376A1 PLANT REGULATORY ELEMENTS AND METHODS OF USE THEREOF 审中-公开
标题翻译：植物调节因子及其使用方法
公开(公告)号：WO2016022376A1
公开(公告)日：2016-02-11
申请号：PCT/US2015/042842
申请日：2015-07-30
申请人： PIONEER HI BRED INTERNATIONAL, INC. , E. I. DU PONT DE NEMOURS AND COMPANY
发明人： DIEHN, Scott , VAN ALLEN, Michelle , LU, Albert
IPC分类号： C12N15/82
CPC分类号： C12N15/8216 , A01H1/00 , C12N2730/00022
摘要： The present disclosure provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for regulatory elements from Lamium Leaf Distortion Associated Virus. A method for expressing a heterologous nucleotide sequence in a plant using the regulatory element sequences disclosed herein is provided. The method comprises transforming a plant or plant cell with a nucleotide sequence operably linked to one of the regulatory elements of the present disclosure.
摘要翻译：本公开提供了用于调节植物中异源核苷酸序列表达的组合物和方法。组合物包括用于来自Lamium Leaf Distortion Associated Virus的调节元件的新型核苷酸序列。提供了使用本文公开的调节元件序列在植物中表达异源核苷酸序列的方法。该方法包括用与本公开的一种调节元件可操作地连接的核苷酸序列转化植物或植物细胞。

39. 发明申请

WO2016007347A1 COMPOSITIONS AND METHODS FOR PRODUCING PLANTS RESISTANT TO GLYPHOSATE HERBICIDE 审中-公开
标题翻译：用于生产耐草甘膦杀虫剂的植物的组合物和方法
公开(公告)号：WO2016007347A1
公开(公告)日：2016-01-14
申请号：PCT/US2015/038767
申请日：2015-07-01
申请人： E. I. DU PONT DE NEMOURS AND COMPANY , PIONEER HI-BRED INTERNATIONAL, INC.
发明人： DJUKANOVIC, Vesna , JONES, Spencer Charles , LIU, Zhan-Bin , LASSNER, Michael , LYZNIK, L. Aleksander
IPC分类号： A01H1/02 , C12N15/113 , C12N15/82
CPC分类号： C12N15/8275 , A01H1/02 , A01H4/008 , C12N15/8213 , C12N15/8241 , C12N2310/20 , C12Y301/00
摘要： The present disclosure includes the production of a mutant plant resistant to an herbicide of the phosphonomethylglycine family, e.g. glyphosate. Compositions and methods are provided for editing a nucleotide sequence of interest in a cell employing a guide polynucleotide/Cas endonuclease system, wherein the Cas endonuclease is guided by a guide polynucleotide to recognize and optionally introduce a double strand break at a specific target site into the genome of a cell. The nucleotide sequence of interest to be edited can be located within or outside the target site that is recognized by a Cas endonuclease. More specifically, compositions and methods are provided for editing an enolpyruvylshikimate-3-phosphate synthase (EPSPS) nucleotide sequence in a cell. The methods and compositions employ a guide polynucleotide/Cas endonuclease system to provide for an effective system for editing EPSPS nucleotide sequences of within the genome of a cell. Also provided are compositions and methods for the production of glyphosate tolerant plant cells, plants explants, seeds and grain.
摘要翻译：本公开内容包括产生对膦酰基甲基甘氨酸家族除草剂具有抗性的突变植物，例如，草甘膦。提供组合物和方法用于使用引导多核苷酸/ Cas内切核酸酶系统编码细胞中的目的核苷酸序列，其中所述Cas内切核酸酶由引导多核苷酸引导以识别并任选地将特定靶位点处的双链断裂引入到细胞的基因组。要编辑的感兴趣的核苷酸序列可以位于由Cas内切核酸酶识别的靶位点内部或外部。更具体地，提供组合物和方法用于在细胞中编辑烯醇丙酮酸莽草酸-3-磷酸合酶（EPSPS）核苷酸序列。方法和组合物采用引导多核苷酸/ Cas内切核酸酶系统来提供用于编辑细胞基因组内的EPSPS核苷酸序列的有效系统。还提供了用于生产耐受草甘膦植物细胞，植物外植体，种子和谷粒的组合物和方法。

40. 发明申请

WO2015191374A1 CONSTRUCTS AND METHODS FOR PLANT TRANSFORMATION 审中-公开
标题翻译：植物转化的构建和方法
公开(公告)号：WO2015191374A1
公开(公告)日：2015-12-17
申请号：PCT/US2015/034343
申请日：2015-06-05
申请人： PIONEER HI BRED INTERNATIONAL INC
发明人： ANAND, Ajith , CHO, Myeong-Je , GORDON-KAMM, William
IPC分类号： C12N15/82
CPC分类号： C12N9/1241 , C12N15/8201 , C12N15/8205 , C12N15/8209 , C12N15/8213 , C12N15/8216 , C12N15/8273 , C12N15/8286 , C12N2800/30 , C12Y207/07
摘要： Compositions and transformation methods to increase the frequency of plants in a population of transformed plants which have a single copy of the target polynucleotide of interest are provided. The frequency of plants in a population of transformed plants containing no contaminating vector backbone sequence may be increased. The methods and compositions provide for a greater number of transgenic events having single copy inserts and no contaminating vector backbone sequence.
摘要翻译：提供了组合物和转化方法，用于增加具有目标目的多核苷酸单拷贝的转化植物群体中植物的频率。可以增加不含污染载体骨架序列的转化植物群体中植物的频率。所述方法和组合物提供更多数量的具有单拷贝插入物并且没有污染载体骨架序列的转基因事件。

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