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31. 发明申请

WO2005121351A3 METHODS FOR TRANSFORMING AND EXPRESSION SCREENING OF FILAMENTOUS FUNGAL CELLS WITH A DNA LIBRARY 审中-公开
公开(公告)号：WO2005121351A3
公开(公告)日：2005-12-22
申请号：PCT/US2005/018871
申请日：2005-05-26
申请人： NOVOZYMES, INC.
发明人： TETER, Sarah , LAMSA, Michael , CHEERY, Joel , WARD, Connie
IPC分类号： C12P21/06
摘要： The present invention relates to methods for expression screening of filamentous fungal transformants, comprising: (a) isolating single colony transformants of a DNA library introduced into E. coli; (b) preparing DNA from each of the single colony E. coli transformants; (c) introducing a sample of each of the DNA preparations of step (b) into separate suspensions of protoplasts of a filamentous fungus to obtain transformants thereof, wherein each transformant contains one or more copies of an individual polynucleotide from the DNA library; (d) growing the individual filamentous fungal transformants of step (c) on selective growth medium, thereby permitting growth of the filamentous fungal transformants, while suppressing growth of untransformed filamentous fungi; and (e) measuring activity or a property of each polypeptide encoded by the individual polynucleotides.

32. 发明申请

WO2005121351A2 METHODS FOR TRANSFORMING AND EXPRESSION SCREENING OF FILAMENTOUS FUNGAL CELLS WITH A DNA LIBRARY 审中-公开
标题翻译：用DNA文库对真菌真菌进行转化和表达筛选的方法
公开(公告)号：WO2005121351A2
公开(公告)日：2005-12-22
申请号：PCT/US2005018871
申请日：2005-05-26
申请人： NOVOZYMES INC
发明人： TETER SARAH , LAMSA MICHAEL , CHEERY JOEL , WARD CONNIE
IPC分类号： C12N1/16 , C12N15/74 , C12P21/06
CPC分类号： C12N15/1037 , C12N15/1086 , C12N15/80 , C40B40/02
摘要： The present invention relates to methods for expression screening of filamentous fungal transformants, comprising: (a) isolating single colony transformants of a DNA library introduced into E. coli; (b) preparing DNA from each of the single colony E. coli transformants; (c) introducing a sample of each of the DNA preparations of step (b) into separate suspensions of protoplasts of a filamentous fungus to obtain transformants thereof, wherein each transformant contains one or more copies of an individual polynucleotide from the DNA library; (d) growing the individual filamentous fungal transformants of step (c) on selective growth medium, thereby permitting growth of the filamentous fungal transformants, while suppressing growth of untransformed filamentous fungi; and (e) measuring activity or a property of each polypeptide encoded by the individual polynucleotides.
摘要翻译：本发明涉及丝状真菌转化体的表达筛选方法，其包括：（a）分离导入大肠杆菌的DNA文库的单个菌落转化体; （b）从每个单个菌落的大肠杆菌转化体制备DNA; （c）将步骤（b）的每种DNA制剂的样品引入到丝状真菌的原生质体的分离悬浮液中以获得其转化体，其中每个转化体含有来自DNA文库的单个多核苷酸的一个或多个拷贝; （d）在选择性生长培养基上生长步骤（c）的单个丝状真菌转化体，从而允许丝状真菌转化体生长，同时抑制未转化的丝状真菌的生长; 和（e）测量由各个多核苷酸编码的每种多肽的活性或性质。

33. 发明申请

WO2005074656A3 POLYPEPTIDES HAVING CELLULOLYTIC ENHANCING ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME 审中-公开
公开(公告)号：WO2005074656A3
公开(公告)日：2005-08-18
申请号：PCT/US2005/003802
申请日：2005-02-04
申请人： NOVOZYMES, INC.
发明人： DOTSON, William , GREENIER, Jennifer , DING, Hanshu
IPC分类号： C12N9/42 , C12N15/56 , C12P1/02
摘要： The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated nucleic acids encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acids as well as methods for producing and using the polypeptides.

34. 发明申请

WO2005056772A1 METHODS FOR ELIMINATING OR REDUCING THE EXPRESSION OF GENES IN FILAMENTOUS FUNGAL STRAINS 审中-公开
标题翻译：消除或减少真菌真菌菌株中基因表达的方法
公开(公告)号：WO2005056772A1
公开(公告)日：2005-06-23
申请号：PCT/US2004/041510
申请日：2004-12-09
申请人： NOVOZYMES INC. , NOVOZYMES A/S
发明人： MAIYURAN, Suchindra , UDAGAWA, Hiroaki , BRODY, Howard
IPC分类号： C12N1/15
CPC分类号： C12P1/02 , C12N15/113 , C12N15/1137 , C12N2310/111 , C12N2310/14 , C12N2310/53
摘要： The present invention relates to methods for reducing or eliminating the expression of a target gene in a filamentous fungal strain, comprising: (a) inserting into the genome of the strain a double-stranded transcribable nucleic acid construct comprising a first nucleotide sequence comprising a promoter operably linked to a homologous coding region of the target gene and a second nucleotide sequence comprising the homologous coding region, or a portion thereof, of the target gene, wherein first and second nucleotide sequences are complementary to each other and the second nucleotide sequence is in reverse orientation relative to the first nucleotide sequence; and (b) inducing production of an interfering RNA encoded by the double-stranded transcribable nucleic acid construct by cultivating the under conditions conducive for production of the interfering RNA; wherein the interfering RNA interacts with RNA transcripts of the target gene to reduce or eliminate expression of the target gene.
摘要翻译：本发明涉及用于减少或消除丝状真菌菌株中靶基因表达的方法，其包括：（a）在菌株的基因组中插入双链可转录核酸构建体，其包含第一核苷酸序列，其包含启动子可操作地连接到靶基因的同源编码区和包含靶基因的同源编码区或其部分的第二核苷酸序列，其中第一和第二核苷酸序列彼此互补，第二个核苷酸序列在相对于第一核苷酸序列的反向取向; 和（b）通过培养有利于产生干扰RNA的条件，诱导由双链可转录核酸构建体编码的干扰RNA的产生; 其中所述干扰RNA与所述靶基因的RNA转录物相互作用以减少或消除所述靶基因的表达。

35. 发明申请

WO2017019490A1 POLYPEPTIDES HAVING ARABINOFURANOSIDASE ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME 审中-公开
标题翻译：具有阿拉伯糖苷酶活性的多肽和编码其的多核苷酸
公开(公告)号：WO2017019490A1
公开(公告)日：2017-02-02
申请号：PCT/US2016/043521
申请日：2016-07-22
申请人： NOVOZYMES INC.
发明人： HARRIS, Paul Vincent , HASANI, Sumati , LIU, Fang
IPC分类号： C12N9/24
CPC分类号： C12N9/2402 , C07K2319/00 , C12Y302/01 , C12Y302/01055
摘要： The present invention relates to isolated polypeptides having arabinofuranosidase activity, catalytic domains, carbohydrate binding modules and polynucleotides encoding the polypeptides, catalytic domains or carbohydrate binding modules. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or carbohydrate binding modules.
摘要翻译：本发明涉及具有阿拉伯呋喃糖苷酶活性的分离多肽，催化结构域，碳水化合物结合模块和编码多肽，催化结构域或碳水化合物结合模块的多核苷酸。本发明还涉及包含多核苷酸的核酸构建体，载体和宿主细胞以及产生和使用多肽，催化结构域或碳水化合物结合模块的方法。

36. 发明申请

WO2014138672A1 CELLOBIOHYDROLASE VARIANTS AND POLYNUCLEOTIDES ENCODING SAME 审中-公开
标题翻译：碳水化合物变体和编码其的多核苷酸
公开(公告)号：WO2014138672A1
公开(公告)日：2014-09-12
申请号：PCT/US2014/022068
申请日：2014-03-07
申请人： NOVOZYMES A/S , NOVOZYMES, INC. , BORCH, Kim , JENSEN, Kenneth , KROGH, Kristian , MCBRAYER, Brett , WESTH, Peter , KARI, Jeppe , OLSEN, Johan , SØRENSEN, Trine , WINDAHL, Michael , XU, Hui
发明人： BORCH, Kim , JENSEN, Kenneth , KROGH, Kristian , MCBRAYER, Brett , WESTH, Peter , KARI, Jeppe , OLSEN, Johan , SØRENSEN, Trine , WINDAHL, Michael , XU, Hui
IPC分类号： C12N9/42 , C12N15/82
CPC分类号： C12N9/2437 , C12N15/8246 , C12N15/8257 , C12P19/02 , C12P19/14 , C12P2203/00 , C12Y302/01091
摘要： The present invention relates to cellobiohydrolase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
摘要翻译：本发明涉及纤维二糖水解酶变体。本发明还涉及编码这些变体的多核苷酸; 核酸构建体，载体和包含多核苷酸的宿主细胞; 以及使用这些变体的方法。

37. 发明申请

WO2014099798A1 POLYPEPTIDES HAVING CELLULOLYTIC ENHANCINC ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME 审中-公开
标题翻译：具有纤维素增强活性的多肽和编码其的多核苷酸
公开(公告)号：WO2014099798A1
公开(公告)日：2014-06-26
申请号：PCT/US2013/075419
申请日：2013-12-16
申请人： NOVOZYMES A/S , NOVOZYMES, INC. , SCHNORR, Kirk , SHAGHASI, Tarana , SWEENEY, Matt
发明人： SCHNORR, Kirk , SHAGHASI, Tarana , SWEENEY, Matt
IPC分类号： C12N9/24 , C12P7/10 , D21C5/00 , C12N9/42 , C12N9/96
CPC分类号： C12N9/2437 , C12N9/0004 , C12N9/0071 , C12N15/1137 , C12N2310/14 , C12P7/10 , C12P19/02 , C12P19/12 , C12P2203/00 , C12Y302/01004 , D21C5/005 , Y02E50/16
摘要： The present invention relates to isolated polypeptides having cellulolytic enhancing activity and polynucleotides encoding the polypeptides, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
摘要翻译：本发明涉及具有纤维素分解增强活性的分离多肽和编码该多肽的多核苷酸，以及编码该多肽的多核苷酸。本发明还涉及包含多核苷酸的核酸构建体，载体和宿主细胞以及产生和使用多肽的方法。

38. 发明申请

WO2014085330A1 3-HYDROXYPROPIONIC ACID PRODUCTION BY RECOMBINANT YEASTS 审中-公开
标题翻译： 3重羟基酸生产重组酵母
公开(公告)号：WO2014085330A1
公开(公告)日：2014-06-05
申请号：PCT/US2013/071696
申请日：2013-11-25
申请人： NOVOZYMES, INC. , BINDEL, Mariah
发明人： BINDEL, Mariah
IPC分类号： C12P7/52 , C12N1/16
CPC分类号： C12P7/42 , C12N1/16 , C12N9/0008 , C12N15/52 , C12Y102/01009 , Y02P20/52
摘要： Provided herein are recombinant yeast cells having an active 3-Hydroxypropionic Acid (3-HP) pathway and further comprising a heterologous polynucleotide encoding a non-phosphorylating NADP-dependent glyceraldehyde-3-phosphate dehydrogenase (GAPN). Also described are methods of using the recombinant yeast cells to produce 3-HP and acrylic acid.
摘要翻译：本文提供具有活性3-羟基丙酸（3-HP）途径并且还包含编码非磷酸化NADP依赖性甘油醛-3-磷酸脱氢酶（GAPN）的异源多核苷酸的重组酵母细胞。还描述了使用重组酵母细胞产生3-HP和丙烯酸的方法。

39. 发明申请

WO2014052630A1 BACTERIAL MUTANTS WITH IMPROVED TRANSFORMATION EFFICIENCY 审中-公开
标题翻译：具有改进效率的细菌菌株
公开(公告)号：WO2014052630A1
公开(公告)日：2014-04-03
申请号：PCT/US2013/061980
申请日：2013-09-26
申请人： NOVOZYMES, INC. , BERKA, Randy , CHERRY, Barbara
发明人： BERKA, Randy , CHERRY, Barbara
IPC分类号： C07K14/32
CPC分类号： C12N15/75 , C07K14/32 , C12P5/007 , C12P5/02 , C12P5/026 , C12P7/04 , C12P13/04 , C12P21/00 , C12P21/02 , Y02E50/343
摘要： Provided herein are Bacillus mutants having improved transformation efficiency, comprising a disruption to an endogenous mecA gene and disruption to an endogenous sinI gene. Also described are methods for producing the mutants, methods for generating transformants using the mutants, and methods for producing a polypeptide or fermentation product using the mutants.
摘要翻译：本文提供具有改善的转化效率的芽孢杆菌突变体，其包含内源性mecA基因的破坏和内源性sinI基因的破坏。还描述了用于产生突变体的方法，使用突变体产生转化体的方法，以及使用突变体产生多肽或发酵产物的方法。

40. 发明申请

WO2013089889A3 CHIMERIC POLYPEPTIDES HAVING BETA-GLUCOSIDASE ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME 审中-公开
标题翻译：具有β-葡糖苷酶活性的多克隆多肽和编码相同多核苷酸的多核苷酸
公开(公告)号：WO2013089889A3
公开(公告)日：2013-08-15
申请号：PCT/US2012057893
申请日：2012-09-28
申请人： NOVOZYMES INC
发明人： WOGULIS MARK , OSBORN DAVID , HEU TIA
IPC分类号： C07K14/435
CPC分类号： C12N9/2445 , C07K2319/00 , C12N15/8257 , C12P19/02 , C12P19/14
摘要： The present invention relates to chimeric polypeptides having beta-glucosidase activity. The present invention also relates to polynucleotides encoding the chimeric polypeptides; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the chimeric polypeptides
摘要翻译：本发明涉及具有β-葡糖苷酶活性的嵌合多肽。本发明还涉及编码嵌合多肽的多核苷酸; 包含所述多核苷酸的核酸构建体，载体和宿主细胞; 和使用嵌合多肽的方法

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