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21. 发明申请

WO2015112182A1 MAIZE EVENT DP-032218-9 AND METHODS FOR DETECTION THEREOF 审中-公开
标题翻译：主要事项DP-032218-9及其检测方法
公开(公告)号：WO2015112182A1
公开(公告)日：2015-07-30
申请号：PCT/US2014/032526
申请日：2014-04-01
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E.I. DUPONT DE NEMOURS & COMPANY , BEATTY, Mary , BRINK, Ken , CRANE, Virginia, Cox , DIEHN, Scott , LU, Albert, Laurence , YOUNG, Gregory, J.
发明人： BEATTY, Mary , BRINK, Ken , CRANE, Virginia, Cox , DIEHN, Scott , LU, Albert, Laurence , YOUNG, Gregory, J.
IPC分类号： C12N15/82 , C12Q1/68
CPC分类号： C12N15/8277 , C12N15/8286 , C12Q1/6895 , Y02A40/162
摘要： The disclosure provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize DP-032218-9 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.
摘要翻译：本公开提供与转基因抗虫玉米植物相关的DNA组合物。还提供了基于插入到玉米基因组中的重组构建体的DNA序列和插入位点侧翼的DNA序列来检测玉米DP-032218-9事件的存在的测定法。提供了用于进行测定的试剂盒和条件。

22. 发明申请

WO2012088227A1 VIRAL PROMOTER, TRUNCATIONS THEREOF, AND METHODS OF USE 审中-公开
公开(公告)号：WO2012088227A1
公开(公告)日：2012-06-28
申请号：PCT/US2011/066389
申请日：2011-12-21
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E. I. DU PONT DE NEMOURS AND COMPANY , DIEHN, Scott , SIMMONS, Carl, R. , LU, Albert Laurence
发明人： DIEHN, Scott , SIMMONS, Carl, R. , LU, Albert Laurence
IPC分类号： C12N15/82
CPC分类号： C12N15/8225 , C12N2730/00043
摘要： The present disclosure provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a promoter. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequence disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.

23. 发明申请

WO2011103154A3 A GREEN TISSUE-PREFERRED PROMOTER FROM MAIZE 审中-公开
标题翻译：绿色组织优先促销者从MAIZE
公开(公告)号：WO2011103154A3
公开(公告)日：2011-10-27
申请号：PCT/US2011025035
申请日：2011-02-16
申请人： PIONEER HI BRED INT , DU PONT , DIEHN SCOTT , LU ALBERT L , SIMS LYNNE E
发明人： DIEHN SCOTT , LU ALBERT L , SIMS LYNNE E
IPC分类号： A01H5/00 , C12N15/29 , C12N15/82
CPC分类号： C07K14/415 , C12N15/8223 , C12N15/8225
摘要： The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a green tissue-preferred promoter from maize. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises stabling incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.
摘要翻译：本发明提供调节植物中异源核苷酸序列表达的组合物和方法。组合物包括用于来自玉米的绿色组织优选启动子的新型核苷酸序列。提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。该方法包括将与本发明的根优选启动子可操作地连接的核苷酸序列掺入到植物细胞的基因组中，并再生表达核苷酸序列的稳定转化的植物。

24. 发明申请

WO2011025860A1 COMPOSITIONS AND METHODS TO CONTROL INSECT PESTS 审中-公开
标题翻译：组合物和方法来控制污染物
公开(公告)号：WO2011025860A1
公开(公告)日：2011-03-03
申请号：PCT/US2010/046762
申请日：2010-08-26
申请人： E. I. DU PONT DE NEMOURS AND COMPANY , BROGLIE, Karen, E. , KRISS, Kevin , LU, Albert, Laurence , MUTHALAGI, Mani , PRESNAIL, James, K.
发明人： BROGLIE, Karen, E. , KRISS, Kevin , LU, Albert, Laurence , MUTHALAGI, Mani , PRESNAIL, James, K.
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： A01N57/16 , A01N63/00 , A01N65/20 , A01N65/44 , C12N15/113 , C12N15/8218 , C12N15/8286 , C12N2310/11 , C12N2310/531 , Y02A40/162
摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a Coleopteran plant pest or a Diabrotica plant pest decrease the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides various target polynucleotides set forth in any one of SEQ ID NOS: 1-236 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is D. virgifera virgifera, D. barber i, D. speciosa, or D. undecimpimctata howardi, Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
摘要翻译：提供了使用沉积元件的方法和组合物，当由有害生物摄入时，例如鞘翅目植物病虫害病毒（Diabrotica plant pest），害虫减少害虫中靶序列的表达。在具体实施方案中，靶序列的表达降低控制害虫，由此方法和组合物能够限制对植物的损害。本发明提供了SEQ ID NO：1-236中任一项所述的各种目标多核苷酸或其活性变体和片段，其中目标害虫中一个或多个序列的表达降低控制害虫（即具有杀虫活性活动）。进一步提供的是沉积元件，当受害虫摄入时，降低靶多肽的水平，从而控制害虫。在具体实施方案中，有害生物是D. virgifera virgifera，D.berber i，D. speciosa或D. undecimpimctata howardi，植物，植物部分，细菌和其它宿主细胞，其包含沉默元件或其活性变体或片段还提供了发明。

25. 发明申请

WO2010141186A1 VIRAL PROMOTER, TRUNCATIONS THEREOF, AND METHODS OF USE 审中-公开
标题翻译：司法促进者，其职权及其使用方法
公开(公告)号：WO2010141186A1
公开(公告)日：2010-12-09
申请号：PCT/US2010/034320
申请日：2010-05-11
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E. I. DUPONT DE NEMOURS & COMPANY , DIEHN, Scott , LU, Albert L. , SIMMONS, Carl R.
发明人： DIEHN, Scott , LU, Albert L. , SIMMONS, Carl R.
IPC分类号： A01H5/00 , C12N15/29 , C12N15/82
CPC分类号： C12N15/8216
摘要： The present disclosure provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a promoter. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequence disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.
摘要翻译：本公开提供了用于调节植物中异源核苷酸序列表达的组合物和方法。组合物包括用于启动子的新型核苷酸序列。提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。该方法包括将与本发明的启动子可操作地连接的核苷酸序列稳定地并入到植物细胞的基因组中，并再生表达核苷酸序列的稳定转化的植物。

26. 发明申请

WO2007024866A3 METHODS AND COMPOSITIONS FOR THE EXPRESSION OF A POLYNUCLEOTIDE OF INTEREST 审中-公开
公开(公告)号：WO2007024866A3
公开(公告)日：2007-03-01
申请号：PCT/US2006/032792
申请日：2006-08-22
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E.I. DU PONT DE NEMOURS AND COMPANY , MCCUTCHEN, Billy, Fred , HAZEL, Christine, B. , LIU, Donglong , LU, Albert, L. , MEHRE, Wayne, J. , OLSON, Paul, D. , WONG, James, F. H.
发明人： MCCUTCHEN, Billy, Fred , HAZEL, Christine, B. , LIU, Donglong , LU, Albert, L. , MEHRE, Wayne, J. , OLSON, Paul, D. , WONG, James, F. H.
IPC分类号： C12N15/82
摘要： Methods and compositions for expressing a polynucleotide of interest are provided. Compositions comprise an enhancer domain set forth in SEQ ID NO: 1, 10, 15, or 16 and active variants and fragments thereof. Further provided are DNA constructs comprising at least one transcriptional enhancer sequence comprising the nucleotide sequence set forth in SEQ ID NO:1, 10, 15, or 16 or an active variant or fragment thereof, operably linked to a heterologous promoter. Such chimeric transcription regulatory regions can be operably linked any polynucleotide of interest. Further provided are cells, plants, plant parts, and germplasm comprising the DNA construct. Methods of using the chimeric transcriptional regulatory region are also provided. In specific embodiments, methods of expressing a polynucleotide of interest, including for example, sequences that confer tolerance to herbicides, and methods to select a cell having the DNA construct are provided.

27. 发明申请

WO2007024782A3 COMPOSITIONS PROVIDING TOLERANCE TO MULTIPLE HERBICIDES AND METHODS OF USE THEREOF 审中-公开
公开(公告)号：WO2007024782A3
公开(公告)日：2007-03-01
申请号：PCT/US2006/032606
申请日：2006-08-22
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E. I. DU PONT DE NEMOURS AND COMPANY , MCCUTCHEN, Billy, fred , CASTLE, Linda, A. , CHICOINE, Timothy, K. , CHO, Hyeon-je , CLAUS, Jon, S. , GREEN, Jerry, M. , GUIDA, Anthony, D., Jr. , HAZEL, Christine, B. , HECKERT, Matthew, J. , HEGSTAD, Jeffrey, M. , HUTCHISON, James, M. , LIU, Donglong , LU, Albert, L. , MEHRE, Wayne, J. , MOY, York , OLSON, Paul, D. , PEEPLES, Kenneth, A. , SAUNDERS, David, W. , VOGT, Mark, D. , WILKINSON, Jack, Q. , WONG, James, F., H.
发明人： MCCUTCHEN, Billy, fred , CASTLE, Linda, A. , CHICOINE, Timothy, K. , CHO, Hyeon-je , CLAUS, Jon, S. , GREEN, Jerry, M. , GUIDA, Anthony, D., Jr. , HAZEL, Christine, B. , HECKERT, Matthew, J. , HEGSTAD, Jeffrey, M. , HUTCHISON, James, M. , LIU, Donglong , LU, Albert, L. , MEHRE, Wayne, J. , MOY, York , OLSON, Paul, D. , PEEPLES, Kenneth, A. , SAUNDERS, David, W. , VOGT, Mark, D. , WILKINSON, Jack, Q. , WONG, James, F., H.
IPC分类号： C12N15/82
摘要： Methods and compositions are provided related to improved plants that are tolerant to more than one herbicide. Particularly, the invention provides plants that are tolerant of glyphosate and are tolerant to at least one ALS inhibitor, and methods of use thereof. The glyphosate/ALS inhibitor-tolerant plants comprise a polynucleotide that encodes a polypeptide that confers tolerance to glyphosate and a polynucleotide that encodes an ALS inhibitor-tolerant polypeptide. In specific embodiments, a plant of the invention expresses a GAT polypeptide and an HRA polypeptide. Methods to control weeds, improve plant yield, and increase transformation efficiencies are provided.

28. 发明申请

WO2006133182A2 INSECT-SPECIFIC PROTEASE RECOGNITION SEQUENCES 审中-公开
标题翻译： INSECT-SPECIFIC PROTEASE识别序列
公开(公告)号：WO2006133182A2
公开(公告)日：2006-12-14
申请号：PCT/US2006/021902
申请日：2006-06-06
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E. I. DUPONT DE NEMOURS & COMPANY , ABAD, Andre R. , CHUN, Gary C. , FLANNAGAN, Ronald D. , HERRMANN, Rafael , LU, Albert L. , MCCUTCHEN, Billy F. , RICE, Janet A. , SCHEPERS, Eric J.
发明人： ABAD, Andre R. , CHUN, Gary C. , FLANNAGAN, Ronald D. , HERRMANN, Rafael , LU, Albert L. , MCCUTCHEN, Billy F. , RICE, Janet A. , SCHEPERS, Eric J.
IPC分类号： C07K14/325 , C12N9/64 , A01N63/02 , C12N15/82
CPC分类号： C12N15/8286 , A01N63/02 , C07K14/325 , C12N9/6472 , Y02A40/162
摘要： Compositions and methods for protecting a plant from an insect pest are provided. Nucleic acid molecules encoding insect protoxins or insect toxins modified to comprise at least one proteolytic activation site that is sensitive to an insect gut protease are provided. Cleavage of a modified insect protoxin at the proteolytic activation site by an insect gut protease produces an active insect toxin in the gut of the insect pest. Cleavage of a modified insect toxin of the invention at a proteolytic activation site results in the production of an active insect toxin in the insect gut that displays improved pesticidal activity relative to the insect toxin that lacks the proteolytic activation site. Methods of using the modified insect protoxin and modified insect toxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin and modified insect toxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds. Insect gut proteases and the nucleic acid molecules that encode them are also disclosed herein.
摘要翻译：提供了保护植物免受害虫侵害的组合物和方法。提供编码昆虫原毒素或昆虫毒素的核酸分子，其被修饰为包含至少一个对昆虫肠道蛋白酶敏感的蛋白水解活化位点。通过昆虫肠蛋白酶在蛋白水解活化位点切割修饰的昆虫原毒素在害虫的肠道中产生活性昆虫毒素。在蛋白水解活化位点切割本发明的修饰的昆虫毒素导致在昆虫肠中产生活性昆虫毒素，其相对于缺乏蛋白水解活化位点的昆虫毒素显示出改善的杀虫活性。提供了使用修饰的昆虫原毒素和修饰的昆虫毒素核酸序列及其编码的多肽以保护植物免受害虫的方法。本发明的具体实施方案还提供了改良的昆虫原毒素和改良的昆虫毒素组合物和制剂，表达盒和转化的植物，植物细胞和种子。昆虫肠道蛋白酶和编码它们的核酸分子也在本文中公开。

29. 发明申请

WO2006083513A2 AN INDUCIBLE DEOXYHYPUSINE SYNTHASE PROMOTER FROM MAIZE 审中-公开
标题翻译：一种可诱导的脱氧吉他合成酶促进剂
公开(公告)号：WO2006083513A2
公开(公告)日：2006-08-10
申请号：PCT/US2006/001001
申请日：2006-01-11
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E. I. DU PONT DE NEMOURS AND COMPANY , DIEHN, Scott , LU, Albert L. , MCCUTCHEN, Billy F. , SIMS, Lynne E. , WARD, Kim R.
发明人： DIEHN, Scott , LU, Albert L. , MCCUTCHEN, Billy F. , SIMS, Lynne E. , WARD, Kim R.
CPC分类号： C12N9/0004 , C12N15/8227 , C12N15/8237 , C12N15/8239
摘要： The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for an inducible promoter for the gene encoding a maize deoxyhypusine synthase. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.
摘要翻译：本发明提供调节植物中异源核苷酸序列表达的组合物和方法。组合物包括用于编码玉米脱氧酪氨酸合成酶的基因的诱导型启动子的新型核苷酸序列。提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。该方法包括将可操作地连接到本发明的根优选启动子的核苷酸序列稳定地并入植物细胞的基因组中，并再生表达核苷酸序列的稳定转化的植物。

30. 发明申请

WO2006076189A2 MAIZE CYCLO1 GENE AND PROMOTER 审中-公开
标题翻译： MAIZE CYCLO1基因和促进剂
公开(公告)号：WO2006076189A2
公开(公告)日：2006-07-20
申请号：PCT/US2006/000153
申请日：2006-01-06
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E. I. DU PONT DE NEMOURS & COMPANY , DIEHN, Scott , LU, Albert L. , SIMS, Lynne E. , WARD, Kim R.
发明人： DIEHN, Scott , LU, Albert L. , SIMS, Lynne E. , WARD, Kim R.
IPC分类号： C12N15/82 , C07K14/415 , A01H5/00
CPC分类号： C12N15/8227 , C07K14/415 , C12N15/8279
摘要： The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a root-preferred promoter for the gene encoding Cyclo1. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises stabling incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence. The present invention also relates to isolated nucleic acids encoding plant cyclotides. The invention relates to the construction of a chimeric gene encoding all or a portion of the plant cyclotides, in sense or antisense orientation, wherein expression of the chimeric gene results in the production of altered levels of plant cyclotides in a transformed host cell.
摘要翻译：本发明提供调节植物中异源核苷酸序列表达的组合物和方法。组合物包括用于编码Cyclo1的基因的根优选启动子的新型核苷酸序列。提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。该方法包括将与本发明的根优选启动子可操作地连接的核苷酸序列掺入到植物细胞的基因组中，并再生表达核苷酸序列的稳定转化的植物。本发明还涉及编码植物环肽的分离的核酸。本发明涉及在正义或反义方向上构建编码全部或部分植物环肽的嵌合基因，其中嵌合基因的表达导致在转化的宿主细胞中产生改变水平的植物环肽。

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