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11. 发明申请

WO1989010401A1 PLASMINOGENE ACTIVATORS WITH INCREASED FIBRIN SELECTIVITY 审中-公开
标题翻译：具有增加纤维素选择性的PLASMINOGENE激活剂
公开(公告)号：WO1989010401A1
公开(公告)日：1989-11-02
申请号：PCT/US1989001255
申请日：1989-03-23
申请人： COLLABORATIVE RESEARCH, INC.
发明人： COLLABORATIVE RESEARCH, INC. , MAO, Jen-I , ABERCROMBIE, David, Miller
IPC分类号： C12N09/48
CPC分类号： C12N9/6462 , C12Y304/21073
摘要： A modified plasminogen activator having greater fibrin selectivity than the unmodified plasminogen activator from which it is derived is provided. The modified plasminogen activator includes at least one of the following domains: a kringle that is more homologous with a kringle of plasminogen than is any kringle of the umodified plasminogen activator; a first hybrid t-PA kringle, the first hybrid kringle being a fusion of a portion of t-PA kringle 1 and a portion of t-PA kringle 2; or a second hybrid t-PA kringle, the second hybrid kringle being a fusion of a portion of t-PA kringle 2 and a portion of an scu-PA kringle.
摘要翻译：提供了具有比未衍生的纤维蛋白溶酶原激活物更高的纤维蛋白选择性的修饰的纤溶酶原激活物。修饰的纤溶酶原激活剂包括以下领域中的至少一个：与改变的纤溶酶原激活物的任何三环相比，其与纤溶酶原的三环体更相似的三环; 第一混合t-PA双绞线，第一混合型三角形是一部分t-PA双绞线1和一部分t-PA三角形2的融合; 或第二混合t-PA三角形，所述第二混合三角形是t-PA三角形2的一部分和一部分scu-PA三角形的融合。

12. 发明申请

WO1989008702A1 IMPROVED METHODS FOR RECOVERY OF TISSUE PLASMINOGEN ACTIVATOR USING CHAOTROPIC AGENTS 审中-公开
标题翻译：改良组织匀浆激活剂回收方法的改进方法
公开(公告)号：WO1989008702A1
公开(公告)日：1989-09-21
申请号：PCT/US1989000936
申请日：1989-03-08
申请人： CODON
发明人： CODON , RICE, Craig , MORSER, Michael, John , GLASER, Charles
IPC分类号： C12N09/48
CPC分类号： C12N9/6459 , C12Y304/21069 , Y10S435/815 , Y10S514/824
摘要： This invention relates to the efficient recovery of tissue plasminogen activator (t-PA) from liquid media and more specifically, to improved methods for recovering intact single-chain t-PA substantially free of degraded t-PA and other non-homologous proteins using chaotropic agents to stabilize high concentrations of t-PA during purification.
摘要翻译：本发明涉及从液体培养基有效回收组织纤溶酶原激活剂（t-PA），更具体地说，涉及用于回收基本上不含降解的t-PA的完整单链t-PA和使用离液序列的其它非同源蛋白质的改进方法在纯化期间稳定高浓度的t-PA的试剂。

13. 发明申请

WO1982004261A1 ISOLATION OF PROTEIN AND PEPTIDE FROM URINE 审中-公开
标题翻译：从尿液中分离蛋白质和肽
公开(公告)号：WO1982004261A1
公开(公告)日：1982-12-09
申请号：PCT/US1982000716
申请日：1982-05-26
申请人： UNIV TENNESSEE
发明人： UNIV TENNESSEE , SLOANE NATHAN H
IPC分类号： C12N09/48
CPC分类号： C07K14/47 , A61K38/00 , Y10S530/828 , Y10S530/834
摘要： Le passage de l'urine humaine au travers d'un lit d'un materiau adsorbant provoque l'adsorbtion de l'urine de substances antineoplastiques, ainsi qu'il a ete determine par des techniques de culture de tissus in vitro utilisant des cellules tumorales humaines. L'adsorbant libere deux fractions antineoplastiques lors d'une elution sequentielle. L'elution de la fraction antineoplastique de faible poids moleculaire est obtenue avec l'acetone aqueuse froide a un pH legerement alcalin. L'elution de la fraction antineoplastique de poids moleculaire eleve est executee ensuite par elution avec un melange froid d'acetone aqueuse et de glycerole a un pH legerement alcalin. Les activites antineoplastiques de ces fractions sont determinees par des techniques de culture de tissus utilisant une variete de cellules neoplastiques humaines. En outre, la fraction de poids moleculaire eleve, la proteine urinaire antineoplastique, inhibe egalement la progression et provoque la regression de certaines cellules tumorales humaines implantees dans la souris nue.
摘要翻译：人尿通过吸附材料床导致通过使用人肿瘤细胞的体外组织培养技术测定的来自抗肿瘤物质的尿液的吸附。连续洗脱后，吸附剂产生两种抗肿瘤成分。低分子量抗肿瘤级分的洗脱是通过在弱碱性pH下用冷的含水丙酮洗脱来完成的。此后，通过在弱碱性pH下用冷的含水丙酮 - 甘油混合物洗脱来完成高分子量抗肿瘤级分的洗脱。通过使用多种人肿瘤细胞的组织培养技术测定这些级分的抗肿瘤活性。此外，高分子量级分，抗肿瘤性尿蛋白也抑制了植入裸鼠的某些人类肿瘤细胞的进展并引起消退。

14. 发明申请

WO1996012800A1 MODIFIED FACTOR VII 审中-公开
标题翻译：修改因子七
公开(公告)号：WO1996012800A1
公开(公告)日：1996-05-02
申请号：PCT/US1995013925
申请日：1995-10-24
申请人： ZYMOGENETICS, INC. , NOVO NORDISK A/S
发明人： ZYMOGENETICS, INC. , NOVO NORDISK A/S , BERKNER, Kathleen, L. , PETERSEN, Lars, Christian , HART, Charles, E. , HEDNER, Ulla , BREGENGAARD, Claus
IPC分类号： C12N09/48
CPC分类号： A61K38/166 , A61K38/49 , C12N9/6437 , C12Y304/21021 , Y10S514/822 , A61K2300/00
摘要： The catalytic active site of Factor VII is modified to produce a compound which effectively interrupts the blood coagulation cascade. The modifications render Factor VIIa substantially unable to activate plasma Factors X or IX. Pharmaceutical compositions of the modified Factor VII are used to treat a variety of coagulation-related disorders.
摘要翻译：因子VII的催化活性位点被修饰以产生有效中断凝血级联的化合物。修饰使得因子VIIa基本上不能激活血浆因子X或IX。修饰的因子VII的药物组合物用于治疗各种凝血相关疾病。

15. 发明申请

WO1995027048A1 A METHOD OF PREPARING RECOMBINANT STAPHYLOKINASE 审中-公开
标题翻译：一种制备重组脂蛋白酶的方法
公开(公告)号：WO1995027048A1
公开(公告)日：1995-10-12
申请号：PCT/CN1995000025
申请日：1995-04-03
申请人： SHANGHAI MEDICAL UNIVERSITY , SONG, Houyan
发明人： SHANGHAI MEDICAL UNIVERSITY
IPC分类号： C12N09/48
CPC分类号： C07K14/31
摘要： The present invention relates to constructing expression vector pSTE-SAK-1 by using staphylokinase amplified by PCR and prokaryotic expression vector, such as PLY-4, and transforming E. coli with pSTE-SAK-1, and inducing the expression of such gene by heating. The engineered bacteria are propagated by fermenting, and destructed. The r-SAK product is purified from the supernatant by a two-step method which includes ion exchange and gel filtration; the purity and the yield of the staphylokinase of the invention are high, and cost low.
摘要翻译：本发明涉及通过PCR扩增的葡萄球菌激酶和PLY-4等原核表达载体和用pSTE-SAK-1转化大肠杆菌来构建表达载体pSTE-SAK-1，并通过加热。工程细菌通过发酵和破坏繁殖。通过两步法从上清中纯化r-SAK产物，其包括离子交换和凝胶过滤; 本发明的葡激酶的纯度和产率高，成本低。

16. 发明申请

WO1994026882A1 PROLINE IMINOPEPTIDASE, PROCESS FOR ITS PREPARATION AND ITS USE IN THE FLAVOURING OF FOOD COMPOSITIONS 审中-公开
标题翻译：精氨酸酶抑制剂，其制备方法及其在食品组合物挥发中的应用
公开(公告)号：WO1994026882A1
公开(公告)日：1994-11-24
申请号：PCT/EP1994001497
申请日：1994-05-09
申请人： QUEST INTERNATIONAL B.V. , BOOT, Jacobus , DEUTZ, Inge, Elisabeth, M. , LEDEBOER, Adrianus, Marinus , LEENHOUTS, Cornelis, Johannes , TOONEN, Maria, Yvonne
发明人： QUEST INTERNATIONAL B.V.
IPC分类号： C12N09/48
CPC分类号： C12Y304/11005 , A21D8/042 , A23C19/063 , A23L5/25 , A23L5/27 , A23L27/24 , C12N9/52
摘要： A novel proline iminopeptidase which is a metal dependent serine peptidase, which is obtainable from Propionibacterium shermanii ATCC 9617 and which has a calculated molecular mass of 45 kDa. The proline iminopeptidase has an amino acid sequence according to the unique sequence of Figure 3. Genetic variants having a homology exceeding 60 % and having the same functionality are comprised by the invention. The proline iminopeptidase can be prepared in large quantities with the use of a genetically modified microorganism. It is used for modifying the flavour of food products, and in particular for rendering the product less bitter.
摘要翻译：作为金属依赖性丝氨酸肽酶的新型脯氨酸亚氨基肽酶，其可从丙酸杆菌ATCC 9617获得，其计算分子量为45kDa。脯氨酸亚氨基肽酶具有根据图3的唯一序列的氨基酸序列。本发明包括具有超过60％的同源性并且具有相同官能度的遗传变体。脯氨酸亚氨基肽酶可以使用遗传修饰的微生物大量制备。它用于改变食品的味道，特别是用于使产品更少的苦味。

17. 发明申请

WO1993015188A1 METHOD FOR DECREASING THE ALLERGENICITY OF PSYLLIUM SEED HUSK BY ENZYME TREATMENT 审中-公开
标题翻译：通过酶处理降低磷酸钙的过敏症的方法
公开(公告)号：WO1993015188A1
公开(公告)日：1993-08-05
申请号：PCT/US1992010797
申请日：1992-12-14
申请人： KELLOGG COMPANY
发明人： KELLOGG COMPANY , NIELSEN, Susan
IPC分类号： C12N09/48
CPC分类号： C07G17/00 , A23L5/25
摘要： Disclosed is a method for reducing the allergenicity of psyllium seed husk by treatment with an enzyme. An aqueous slurry of psyllium seed husk is treated with the enzyme under conditions sufficient to inactivate the allergenic protein thus reducing the allergenicity of the psyllium seed husk.

18. 发明申请

WO1991006637A1 SUBTILISIN MUTANTS 审中-公开
标题翻译：分支突变体
公开(公告)号：WO1991006637A1
公开(公告)日：1991-05-16
申请号：PCT/US1990006084
申请日：1990-10-26
申请人： GENENCOR INTERNATIONAL, INC.
发明人： GENENCOR INTERNATIONAL, INC. , CALDWELL, Robert, Mark , ESTELL, David, Aaron , GRAYCAR, Thomas, Paul
IPC分类号： C12N09/48
CPC分类号： C11D3/38609 , C11D3/386 , C12N9/14 , C12N9/16 , C12N9/20 , C12N9/50 , C12N9/54 , C12N15/102 , C12N15/75 , C12P7/6418 , C12R1/07
摘要： Novel carbonyl hydrolase mutants derived from the DNA sequences of naturally-occurring or recombinant non-human carbonyl hydrolases are disclosed. The mutant carbonyl hydrolases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant carbonyl hydrolase to generate the substitution of one or more amino acid residues in the amino acid sequence of a precursor carbonyl hydrolase. Such mutant carbonyl hydrolases have porperties which are different from those of the precursor hydrolase and are especially useful in detergent formulations. The substituted amino acid residues correspond to position +123 and/or +274 in Bacillus amyloliquefaciens subtilisin.
摘要翻译：公开了衍生自天然存在或重组非人羰基水解酶的DNA序列的新型羰基水解酶突变体。突变体羰基水解酶通常通过体外修饰编码天然存在或重组羰基水解酶的前体DNA序列获得，以产生前体羰基水解酶的氨基酸序列中一个或多个氨基酸残基的取代。这种突变型羰基水解酶具有与前体水解酶不同的杂交，并且在洗涤剂配方中特别有用。取代的氨基酸残基对应于解淀粉芽孢杆菌枯草杆菌蛋白酶中的位置+123和/或+ 274。

19. 发明申请

WO1989005347A1 METHOD TO SOLUBILIZE TISSUE PLASMINOGEN ACTIVATOR 审中-公开
标题翻译：溶解组织培养基激活剂的方法
公开(公告)号：WO1989005347A1
公开(公告)日：1989-06-15
申请号：PCT/US1988004402
申请日：1988-12-09
申请人： INVITRON CORPORATION
发明人： INVITRON CORPORATION , DUFFY, Stephen, A. , PRIOR, Christopher, P. , SCOTT, Randal, W.
IPC分类号： C12N09/48
CPC分类号： C12N9/6459 , A61K9/0019 , A61K38/00 , A61K47/12 , A61K47/183 , B01D15/12 , B01D15/34 , C12Y304/21069
摘要： Practical amounts of tissue plasminogen activator (tPA) whether secreted by cells naturally producing it or prepared by recombinant means can be solubilized by providing, in aqueous medium at pH 5-8 a solubilizing amount of a basic amino acid optionally and preferably in the presence of a citric acid moiety. The ability to solubilize tPA at concentrations of up to 50 mg is significant as an aid in permitting smaller volumes for purification and permitting single injections of the drug as opposed to intravenous administration.

20. 发明申请

WO1989000191A1 NOVEL THROMBOLYTIC PROTEINS 审中-公开
标题翻译：新型凝血酶蛋白
公开(公告)号：WO1989000191A1
公开(公告)日：1989-01-12
申请号：PCT/US1988002263
申请日：1988-07-06
申请人： GENETICS INSTITUTE, INC.
发明人： GENETICS INSTITUTE, INC. , LARSEN, Glenn, R. , AHERN, Tim , LANGER-SAFER, Pennina
IPC分类号： C12N09/48
CPC分类号： C12N9/6459 , C12Y304/21069
摘要： Thrombolytic proteins are disclosed which have tissue plasminogen-type activity. The proteins are characterized by modification of the tPA peptide sequence. Methods for making these proteins are disclosed as are therapeutic compositions containing same.
摘要翻译：公开了具有组织纤溶酶原型活性的溶血蛋白。蛋白质的特征是修饰tPA肽序列。公开了制备这些蛋白质的方法，其含有含有它们的治疗组合物。

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