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    • 13. 发明申请
    • METHOD AND APPARATUS FOR ANALYZING INDIVIDUAL CELLS OR PARTICULATES USING FLUORESCENT QUENCHING AND/OR BLEACHING
    • 用于分析使用荧光淬火和/或漂白的个体细胞或颗粒的方法和设备
    • WO2009117683A2
    • 2009-09-24
    • PCT/US2009/037845
    • 2009-03-20
    • ABBOTT POINT OF CARELEVINE, Robert, A.WARDLAW, Stephen, C.LALPURIA, Niten, V.UNFRICHT, Darryn, W.
    • LEVINE, Robert, A.WARDLAW, Stephen, C.LALPURIA, Niten, V.UNFRICHT, Darryn, W.
    • G01N21/64G01N15/14G01N33/50
    • G01N33/49G01N15/1463G01N15/1475G01N21/6428G01N21/6456G01N33/48G01N33/5091G01N2015/008G01N2015/1477G01N2021/6432G01N2021/6439G01N2201/062
    • A method for analyzing a blood sample is provided that includes the steps of: a) providing a blood sample having one or more first constituents and one or more second constituents, which second constituents are different from the first constituents; b) depositing the sample into an analysis chamber adapted to quiescently hold the sample for analysis, the chamber defined by a first panel and a second panel, both of which panels are transparent; c) admixing a colorant with the sample, which colorant is operative to cause the first constituents and second constituents to fluoresce upon exposure to predetermined first wavelengths of light, and which colorant is operative to absorb light at one or more predetermined second wavelengths of light; d) illuminating at least a portion of the sample containing the first constituents and the second constituents at the first wavelengths and at the second wavelengths; e) imaging the at least a portion of the sample, including producing image signals indicative of fluorescent emissions from the first constituents and the second constituents and the optical density of the first constituents and the second constituents; f) determining a fluorescence value for each the first constituents and second constituents using the image signals; g) determining an optical density value for each of the first constituents and second constituents, which optical density is a function of the colorant absorbed by the constituents, using the image signals; and h) identifying the first constituents and the second constituents using the determined fluorescence and optical density values.
    • 提供了一种用于分析血液样本的方法,其包括以下步骤:a)提供具有一个或多个第一成分和一个或多个第二成分的血液样本,该第二成分不同于第一成分 成分; b)将样品沉积到分析室中,该分析室适于静止地保持样品用于分析,该室由第一面板和第二面板限定,这两个面板都是透明的; c)将着色剂与样品混合,该着色剂可操作以在暴露于预定的第一波长的光时使第一组分和第二组分发荧光,并且该着色剂可操作以吸收一种或多种预定的第二波长的光; d)以第一波长和第二波长照射包含第一组分和第二组分的至少一部分样品; e)对样本的至少一部分进行成像,包括产生指示来自第一成分和第二成分的荧光发射以及第一成分和第二成分的光密度的图像信号; f)使用图像信号确定每个第一成分和第二成分的荧光值; g)使用所述图像信号确定所述第一成分和第二成分中的每一个的光密度值,所述光密度是由所述成分吸收的所述着色剂的函数; 和h)使用所确定的荧光和光密度值来识别第一组分和第二组分。