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91. 发明申请

WO2011038360A1 MULTIPLEX (+/-) STRANDED ARRAYS AND ASSAYS FOR DETECTING CHROMOSOMAL ABNORMALITIES ASSOCIATED WITH CANCER AND OTHER DISEASES 审中-公开
标题翻译：用于检测与癌症和其他疾病相关的染色体异常的多重（+/-）条带阵列和测定
公开(公告)号：WO2011038360A1
公开(公告)日：2011-03-31
申请号：PCT/US2010/050431
申请日：2010-09-27
申请人： SIGNATURE GENOMICS LABORATORIES LLC , MCDANIEL, Lisa , BALLIF, Blake , SCHULTZ, Roger , TEBBS, Brice , BEJJANI, Bassem , SHAFFER, Lisa
发明人： MCDANIEL, Lisa , BALLIF, Blake , SCHULTZ, Roger , TEBBS, Brice , BEJJANI, Bassem , SHAFFER, Lisa
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q2565/519 , C12Q2565/513 , C12Q2539/101
摘要： Multiplex (+/-) stranded analyses, such as array comparative genomic hybridization (aCGH), are provided for detecting chromosomal rearrangements associated with cancer and other diseases. For example, an illustrative multiplex array for CGH includes discrete plus (+) strand and minus (-) strand DNA probes, complementary to each other but separable on the CGH array. The minus (-) strand DNA probes recover diagnostic information lost to conventional microarrays, since many genes transcribe from the minus (-) strand. In an illustrative system, patient and control DNA samples are prepared for CGH by amplification and labeling using comprehensive primers that generate both plus (+) strands and minus (-) strands of DNA in the samples. The breakpoints of a translocated chromosome may be detected on a multiplex microarray by DNA probes of one polarity, while DNA copy number changes associated with the translocation region may be detected by corresponding DNA probes of the complementary polarity. Related methods for identifying translocation partner genes are also provided.
摘要翻译：提供多重（+/-）链分析，如阵列比较基因组杂交（aCGH），用于检测与癌症和其他疾病相关的染色体重排。例如，用于CGH的说明性多重阵列包括彼此互补但在CGH阵列上可分离的离散加（+）链和负（ - ）链DNA探针。负（ - ）链DNA探针恢复常规微阵列损失的诊断信息，因为许多基因从负（ - ）链转录。在说明性系统中，通过使用在样品中产生加（+）链和负（ - ）链DNA的综合引物进行扩增和标记来制备用于CGH的患者和对照DNA样品。可以通过一个极性的DNA探针在多重微阵列上检测易位染色体的断点，而与易位区域相关的DNA拷贝数变化可以通过互补极性的相应DNA探针检测。还提供了用于鉴定易位配偶体基因的相关方法。

92. 发明申请

WO2008073378A3 HIGH THROUGHPUT DNA SEQUENCING METHOD AND APPARATUS 审中-公开
标题翻译：高通量DNA测序方法和装置
公开(公告)号：WO2008073378A3
公开(公告)日：2009-04-02
申请号：PCT/US2007025242
申请日：2007-12-11
申请人： THOMAS JEFFERSON UNIVERSITY ME , KHAN RISHI LEE , SCHWABER JAMES STEPHEN
发明人： KHAN RISHI LEE , SCHWABER JAMES STEPHEN
IPC分类号： C12P19/34
CPC分类号： C12Q1/6869 , B01L3/5027 , C12Q2565/619 , C12Q2565/513 , C12Q2565/301
摘要： The present invention relates to a method for high throughput nucleic acid sequencing using a multi-bead flow cell and pyrophosphate sequencing, a sequencer capable of performing this method, and a kit of the pyrophosphate sequencing reagents.
摘要翻译：本发明涉及使用多珠流式细胞和焦磷酸测序的高通量核酸测序方法，能够进行该方法的测序仪和焦磷酸测序试剂盒。

93. 发明申请

WO2008080531A3 METHOD, DEVICE, AND KIT FOR ANALYZING A LIQUID SAMPLE 审中-公开
标题翻译：用于检验液体试验的方法，装置和试剂盒
公开(公告)号：WO2008080531A3
公开(公告)日：2008-12-31
申请号：PCT/EP2007010895
申请日：2007-12-12
申请人： ADVALYTIX AG , GAUER CHRISTOPH
发明人： GAUER CHRISTOPH
IPC分类号： B01J19/00 , C12Q1/68 , G01N33/68
CPC分类号： G01N33/6845 , C12Q1/6837 , G01N33/54306 , C12Q2565/513
摘要： The invention relates to a method for analyzing a liquid sample as to the presence of at least one of at least two predetermined, different types of sample macromolecules, the probability that said at least two predetermined types of different sample macromolecules are present in the sample being less than 20 percent. In said method, a support is provided that comprises at least one reaction center to which at least two types of probe macromolecules are bound. Said at least two types of probe macromolecules are complementary to the at least two predetermined types of different sample macromolecules in such a way as to be able to specifically react therewith. A marking step is carried out for marking at least the predetermined sample macromolecules, the sample is brought in contact with the at least one reaction center such that a reaction can take place between the predetermined sample macromolecules and the respective complementary probe macromolecules, uncombined sample material is removed, and the presence of sample macromolecules that are specifically bound to the at least one reaction center is verified. The invention further relates to a device and a kit for carrying out the method according to the invention as well as a method for producing a device according to the invention.
摘要翻译：本发明涉及一种方法，用于测试的液体样品的至少两个预定的不同品种的样品的大分子中的至少一个的存在，其中所述至少两个预定类型的不同样品的大分子各具有小于20％的样品中存在的概率并且其中一个托架设有至少一个反应中心，附连到至少两种类型的探针的大分子，其是互补的以这样的方式向至少两个预定类型的不同样品的大分子，它们可以特异性地与反应，标记步骤用于标记至少所述预定样本的大分子，样品被放置在这样的方式与接触的至少一个反应中心中进行，所述预定样本的大分子与各自的互补探针的大分子之间的反应未结合的样品材料被去除并且测试了与至少一个反应中心特异性结合的样品大分子的存在。所述Erfmdung还涉及一种装置和用于执行本发明的方法和根据本发明制造器件的方法的试剂盒。

94. 发明申请

WO2006138257A3 SINGLE MOLECULE ARRAYS FOR GENETIC AND CHEMICAL ANALYSIS 审中-公开
标题翻译：用于遗传和化学分析的单分子阵列
公开(公告)号：WO2006138257A3
公开(公告)日：2008-12-11
申请号：PCT/US2006022895
申请日：2006-06-13
申请人： CALLIDA GENOMICS INC , DRMANAC RADOJE , CALLOW MATTHEW J , DRMANAC SNEZANA , HAUSER BRIAN K , YEUNG GEORGE
发明人： DRMANAC RADOJE , CALLOW MATTHEW J , DRMANAC SNEZANA , HAUSER BRIAN K , YEUNG GEORGE
IPC分类号： C12Q1/68 , C07H21/04 , C12M1/00 , C12M1/36
CPC分类号： C12Q1/6874 , C07H21/04 , C07K1/047 , C12Q1/6806 , C12Q1/682 , C12Q1/6837 , C12Q1/6869 , C12Q2525/151 , C12Q2525/313 , C12Q2531/125 , C12Q2565/513 , G01N15/1404 , G01N15/1434 , Y10S977/778 , Y10S977/789 , Y10S977/792 , Y10S977/88 , Y10S977/882 , C12Q2521/307 , C12Q2525/161 , C12Q2535/122 , C12Q2563/179 , C12Q2565/514
摘要： Random arrays of single molecules are provided for carrying out large scale analyses, particularly of biomolecules, such as genomic DNA, cDNAs, proteins, and the like. In one aspect, arrays of the invention comprise concatemers of DNA fragments that are randomly disposed on a regular array of discrete spaced apart regions, such that substantially all such regions contain no more than a single concatemer. Preferably, such regions have areas substantially less than 1 µm2 and have nearest neighbor distances that permit optical resolution of on the order of 109 single molecules per cm2. Many analytical chemistries can be applied to random arrays of the invention, including sequencing by hybridization chemistries, sequencing by synthesis chemistries, SNP detection chemistries, and the like, to greatly expand the scale and potential applications of such techniques.
摘要翻译：提供单个分子的随机阵列用于进行大规模分析，特别是生物分子，例如基因组DNA，cDNAs，蛋白质等。在一个方面，本发明的阵列包括随机设置在离散间隔开的区域的规则阵列上的DNA片段的并列物质，使得基本上所有这些区域都包含不超过单个并联体。优选地，这样的区域具有基本上小于1μm2的面积，并且具有最近的相邻距离，其允许每厘米每分钟109个单分子的光学分辨率。许多分析化学物质可以应用于本发明的随机阵列，包括杂交化学测序，合成化学测序，SNP检测化学等，以大大扩展这些技术的规模和潜在应用。

95. 发明申请

WO2008143627A2 TARGETED WHOLE GENOME AMPLIFICATION METHOD FOR IDENTIFICATION OF PATHOGENS 审中-公开
标题翻译：目标识别病原体的全基因扩增方法
公开(公告)号：WO2008143627A2
公开(公告)日：2008-11-27
申请号：PCT/US2007/020045
申请日：2007-09-14
申请人： IBIS BIOSCIENCES, INC. , ECKER, David, J. , ESHOO, Mark, W.
发明人： ECKER, David, J. , ESHOO, Mark, W.
IPC分类号： A61K31/4741
CPC分类号： C12Q1/6806 , A61K31/4741 , C12P19/34 , C12Q1/68 , C12Q1/6848 , C12Q1/686 , C12Q1/689 , H01J49/0027 , H01J49/40 , Y02A50/473 , C12Q2565/627 , C12Q2537/143 , C12Q2531/119 , C12Q2565/513 , C12Q2563/167
摘要： The methods disclosed herein relate to methods and compositions for amplifying nucleic acid sequences, more specifically, from nucleic acid sequences of pathogens by targeted whole genome amplification.
摘要翻译：本文公开的方法涉及用于通过靶向全基因组扩增来扩增核酸序列，更具体地从病原体的核酸序列扩增的方法和组合物。

96. 发明申请

WO2007120208A2 NANOGRID ROLLING CIRCLE DNA SEQUENCING 审中-公开
标题翻译： NANOGRID滚动圆片DNA序列
公开(公告)号：WO2007120208A2
公开(公告)日：2007-10-25
申请号：PCT/US2006/044209
申请日：2006-11-14
申请人： PRESIDENT AND FELLOWS OF HARVARD COLLEGE , CHURCH, George, M. , PORRECA, Gregory, J. , SHENDURE, Jay , ROSENBAUM, Abraham
发明人： CHURCH, George, M. , PORRECA, Gregory, J. , SHENDURE, Jay , ROSENBAUM, Abraham
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q2565/513 , C12Q2563/155 , C12Q2531/125
摘要： The present invention relates to methods for sequencing a polynucleotide immobilized on an array having a plurality of specific regions each having a defined diameter size, including synthesizing a concatemer of a polynucleotide by rolling circle amplification, wherein the concatemer has a cross-sectional diameter greater than the diameter of a specific region, immobilizing the concatemer to the specific region to make an immobilized concatemer, and sequencing the immobilized concatemer.
摘要翻译：本发明涉及用于对固定在阵列上的多核苷酸进行测序的方法，所述阵列具有多个具有限定直径尺寸的特定区域，包括通过滚圆扩增来合成多核苷酸的连接体，其中所述并联体的横截面直径大于特定区域的直径，将连接体固定到特定区域以制备固定化的连接体，并测序固定化的连接体。

97. 发明申请

WO2007076132A3 COMPOSITIONS COMPRISING ORIENTED, IMMOBILIZED MACROMOLECULES AND METHODS FOR THEIR PREPARATION 审中-公开
标题翻译：包含靶向的固定化大分子的组合物及其制备方法
公开(公告)号：WO2007076132A3
公开(公告)日：2007-09-07
申请号：PCT/US2006049279
申请日：2006-12-22
申请人： NANOSTRING TECHNOLOGIES INC , FERREE SEAN M , DUNAWAY DWAYNE L
发明人： FERREE SEAN M , DUNAWAY DWAYNE L
IPC分类号： C12N11/00 , C12N11/04 , C12N11/16
CPC分类号： B82Y5/00 , C12Q1/6834 , C12Q2565/513 , G01N33/54353 , Y10T428/31768 , Y10T428/31971
摘要： The present invention provides methods and compositions that facilitate the identification of structures of a variety of macromolecules. For instance, the present invention provides methods for the selective immobilization of macromolecules in an extended or oriented state. The present invention also provides compositions comprising macromolecules selectively immobilized in extended or oriented states.
摘要翻译：本发明提供了有助于鉴定各种大分子的结构的方法和组合物。例如，本发明提供了以扩展或定向状态选择性固定大分子的方法。本发明还提供了包含以扩展或取向状态选择性固定的大分子的组合物。

98. 发明申请

WO2007059839A9 METHOD, DEVICE AND KIT FOR STUDYING MACROMOLECULES IN A SAMPLE 审中-公开
标题翻译：方法，装置及KIT FOR大分子在样本调查
公开(公告)号：WO2007059839A9
公开(公告)日：2007-07-26
申请号：PCT/EP2006010283
申请日：2006-10-25
申请人： ADVALYTIX AG , GAUER CHRISTOPH , MANN WOLFGANG
发明人： GAUER CHRISTOPH , MANN WOLFGANG
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837 , C12Q2565/513 , C12Q2565/543 , C12Q2537/143
摘要： The invention relates to a method for studying macromolecules in a sample, in which a support having at least one reaction centre is provided to which are bound at least two types of known macromolecules as probe molecules, macromolecules of the sample which are to be identified are labelled, the sample having at least one reaction centre is brought into contact in such a manner that a reaction between at least one type of the bound macromolecules with the identifying macromolecules can take place, when a specific reaction between macromolecules to be identified with one type of bound macromolecule is possible, unbound sample material is removed and the presence of sample macromolecules specifically bound to the at least one reaction centre is tested. The invention further relates to a device and a kit for carrying out the method of the invention and to a method for producing a device of the invention.
摘要翻译：本发明涉及一种用于大分子的样品中的分析，其中，载体设有至少一个反应中心的方法，附连到所述至少两种类型的公知的大分子作为探针分子被检测样品中的大分子标记，与所述至少一个样品反应中心是这样接触时，可存在至少一种类型的与检测的大分子结合的大分子之间的反应，当检测到的大分子之间的特异性反应是可能的与各种结合的大分子，未结合的样品材料被去除的和在存在至少一个反应位点特异性结合的样品的大分子被选中。本发明还涉及一种装置和用于根据本发明和根据本发明的制造装置的方法实施该方法的试剂盒。

99. 发明申请

WO2005045076A3 IMPROVED ACCURACY ARRAY ASSAY SYSTEM AND METHOD 审中-公开
标题翻译：改进的准确的数组测试系统和方法
公开(公告)号：WO2005045076A3
公开(公告)日：2005-06-30
申请号：PCT/US2004036249
申请日：2004-10-28
申请人： BECKMAN COULTER INC , SILZEL JOHN , OFFORD KEITH , MAURER GARRICK
发明人： SILZEL JOHN , OFFORD KEITH , MAURER GARRICK
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837 , C12Q2565/513
摘要： An array device 10 and 100 for detection of targets has a plurality of detection zones 2A, 2B, and 2C containing different detection spots 14, 16, 18, 20, 22, 114, 116, 118, 120 and 122 in a predetermined pattern, such as edge effects. The detection spots 14, 16, 18, 20, 22, 114, 116, 118, 120 and 122 are randomized to reduce inaccuracies resulting from spatial effects. Preferably, no two detection zones 2A, 2B, and 2C have the same pre-determined pattern. The device can be provided with a readable code to allow a detector to determine the predetermined pattern used for the array.
摘要翻译：用于检测目标的阵列装置10和100具有包含预定图案的不同检测点14,16,18,20,22,114,116,118,120和122的多个检测区域2A，2B和2C，如边缘效应。检测点14,16,18,20,22,114,116,118,120和122被随机化以减少由空间效应引起的不准确性。优选地，没有两个检测区域2A，2B和2C具有相同的预定图案。可以向设备提供可读代码以允许检测器确定用于阵列的预定图案。

100. 发明申请

WO2005017193A1 A RAPID METHOD TO DETECT NUCLEIC ACID MOLECULES 审中-公开
标题翻译：检测核酸分子的快速检测方法
公开(公告)号：WO2005017193A1
公开(公告)日：2005-02-24
申请号：PCT/CN2003/000722
申请日：2003-08-27
申请人： TSINGHUA UNIVERSITY , CAPITAL BIOCHIP COMPANY, LTD. , WANG, Dong , LI, Gang , MA, Xuemei , LIU, Chengxun , ZHOU, Yuxiang , CHENG, Jing
发明人： WANG, Dong , LI, Gang , MA, Xuemei , LIU, Chengxun , ZHOU, Yuxiang , CHENG, Jing
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , C12Q1/6837 , C12Q2565/501 , C12Q2565/513 , C12Q2565/507
摘要： This invention relates to the field of detecting nucleic acid molecules using microarrays. The invention provides a method for detecting a target nucleic acid molecule in a biological sample by hybridizing a cell lysate directly probes immobilized on microarrays without any nucleic acid purification.
摘要翻译：本发明涉及使用微阵列检测核酸分子的领域。本发明提供了一种用于通过将固定在微阵列上的细胞裂解物直接杂交而不进行任何核酸纯化来检测生物样品中的靶核酸分子的方法。

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