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91. 发明申请

WO0120008A3 VASCULAR-SPECIFIC PROMOTERS 审中-公开
标题翻译：血管特异性促进剂
公开(公告)号：WO0120008A3
公开(公告)日：2001-08-09
申请号：PCT/EP0006248
申请日：2000-07-03
申请人： UNIV LEIDEN , SCARPELLA ENRICO , HOGE MEPPELINK ANNEKE HF , MEIJER ANNEMARIE H
发明人： HOGE J HARRY C DI , SCARPELLA ENRICO , HOGE-MEPPELINK ANNEKE HF , MEIJER ANNEMARIE H
IPC分类号： C07K14/415 , C12N1/21 , C12N15/82 , A01H5/00 , C12N1/00
CPC分类号： C07K14/415 , C12N15/8223 , C12N15/8237 , C12N15/8238 , C12N15/8239 , C12N15/8279 , C12N15/8286 , Y02A40/162
摘要： A DNA fragment upstream of the rice Oshox1 gene or portions or variants of the DNA fragment wherein the DNA fragment or portions or variants thereof are capable of promoting vascular expression of an associated DNA sequence upon reintroduction into a plant, typically wherein vascular expression of the associated DNA sequence enables production of an antipathogenic protein.
摘要翻译：水稻Oshox1基因上游的DNA片段或DNA片段的部分或变体，其中DNA片段或其部分或变体能够在重新引入植物中时促进相关DNA序列的血管表达，通常其相关DNA序列的血管表达能够产生抗病原性蛋白质。

92. 发明申请

WO0134821A2 CHEMICAL INDUCIBLE PROMOTER USED TO OBTAIN TRANSGENIC PLANTS WITH A SILENT MARKER AND ORGANISMS AND CELLS AND METHODS OF USING SAME FOR SCREENING FOR MUTATIONS 审中-公开
标题翻译：用于获得具有无机标记和有机体和细胞的转基因植物的化学诱导促进剂及其筛选用于筛选的方法
公开(公告)号：WO0134821A2
公开(公告)日：2001-05-17
申请号：PCT/US0031070
申请日：2000-11-13
申请人： UNIV ROCKEFELLER , ZUO JIANRU , CHUA NAM HAI
发明人： ZUO JIANRU , CHUA NAM-HAI
IPC分类号： A01H1/00 , C12N5/10 , C12N15/09 , C12N15/82 , A01H5/00 , C12Q1/02 , C12Q1/68
CPC分类号： C12N15/8274 , C12N15/8209 , C12N15/8237 , C12N15/8238 , C12N15/8242 , C12N15/8295
摘要： Disclosed is a chemically inducible promoter for transforming plants or plant cells with genes which are regulatable by adding the plants or cells to a medium containing an inducer or by removing them from such medium. The promoter is inducible by a glucocorticoid, estrogen or inducer not endogenous to plants. Such promoters may be used with any plant genes that can promote shoot regeneration and development to induce shoot formation in the presence of a glucocorticoid, estrogen or inducer. The promoter may be used with antibiotic or herbicide resistance genes or other genes which are regulatable by the presence or absence of a given inducer. Also presented are organisms or cells comprising a gene wherein the natural promoter of the gene is disrupted and the gene is placed under the control of a transgenic inducible promoter. These organisms and cells and their progeny are useful for screening for conditional gain of function and loss of function mutations.
摘要翻译：公开了一种化学诱导型启动子，用于通过将植物或细胞添加到含有诱导物的培养基中或通过从这种培养基中除去可调节基因来转化植物或植物细胞。启动子可通过糖皮质激素，雌激素或对植物无内源性的诱导物诱导。这样的启动子可以与任何植物基因一起使用，所述植物基因可以在糖皮质激素，雌激素或诱导物的存在下促进芽再生和发育以诱导芽形成。启动子可以与抗生素或除草剂抗性基因或其它可通过给定诱导物的存在或不存在而调节的基因一起使用。还提供了包含基因的生物体或细胞，其中基因的天然启动子被破坏并且将基因置于转基因诱导型启动子的控制下。这些生物和细胞及其后代可用于筛选功能条件增加和功能突变丧失。

93. 发明申请

WO0102593A3 CONTROL OF GENE EXPRESSION IN EUKARYOTIC CELLS 审中-公开
标题翻译：原核细胞中基因表达的控制
公开(公告)号：WO0102593A3
公开(公告)日：2001-05-17
申请号：PCT/US0018444
申请日：2000-06-30
申请人： CALGENE LLC , MCBRIDE KEVIN , OULMASSOV TIM N , MILLER PAULA C , ANDERSON JOHN C , CROSSLAND LYLE D , ADAMS TOM , GAVRIAS VICKY
发明人： MCBRIDE KEVIN , OULMASSOV TIM N , MILLER PAULA C , ANDERSON JOHN C , CROSSLAND LYLE D , ADAMS TOM , GAVRIAS VICKY
IPC分类号： A01H5/00 , A01K67/027 , A61K31/7088 , A61K48/00 , A61P31/00 , C07D307/33 , C07H21/04 , C12N1/19 , C12N5/04 , C12N5/10 , C12N9/18 , C12N15/09 , C12N15/81 , C12N15/82 , C12N15/85 , C12Q1/02 , C12Q1/68 , A61K31/34 , A61K31/365 , C12N15/67
CPC分类号： C07D307/33 , A61K48/00 , C12N15/81 , C12N15/8214 , C12N15/8216 , C12N15/8217 , C12N15/8238 , C12N15/85 , C12N2830/00 , C12N2830/001 , C12N2830/002 , C12N2830/15 , C12N2830/30 , C12N2830/36 , C12N2840/105 , C12N2840/20 , C12N2840/203
摘要： DNA constructs and other compositions and methods for controlling gene expression in eukaryotic cells and organisms are derived from bacterial quorum sensing systems. One or more cis elements from the luxI promoter (" lux box") or a functionally similar sequence are incorporated in a eukaryotic promoter. A receptor protein from the LuxR family of transcriptional regulators, upon binding an acylated homoserine lactone (AHL) compound, interacts with the lux box, modulating the activity of the promoter.
摘要翻译：用于控制真核细胞和生物体中的基因表达的DNA构建体和其它组合物和方法源自细菌群体感测系统。来自LuxI启动子（“lux”框）“的一个或多个顺式元件或功能上相似的序列并入真核启动子中。转录调节因子的LuxR家族的受体蛋白在结合酰化的高丝氨酸内酯（AHL）化合物后，与lux框相互作用，调节启动子的活性。

94. 发明申请

WO01027298A2 SCLEROTINIA-INDUCIBLE GENES AND PROMOTERS AND THEIR USES 审中-公开
标题翻译： SCLEROTINIA - 不可遗传的基因和促销者及其用途

公开(公告)号：WO01027298A2

公开(公告)日：2001-04-19

申请号：PCT/US2000/028489

申请日：2000-10-13

IPC分类号： C07K14/415 , C12N15/29 , C12N15/82 , A01H5/00 , C12N5/10

CPC分类号： C07K14/415 , C12N15/8237 , C12N15/8238 , C12N15/8239 , C12N15/8282

摘要： Compositions and methods to aid in protecting plants from invading pathogenic organisms are provided. The compositions of the invention comprise anti-pathogenic nucleotide sequences, including their promoters, and polypeptides encoded by the anti-pathogenic nucleic acid sequences. The compositions find use in methods for reducing or eliminating damage to plants caused by plant pathogens. Transformed plants, plant cells, tissues, and seed are also provided having enhanced disease resistance.

摘要翻译：提供了有助于保护植物免受入侵病原生物体的组合物和方法。本发明的组合物包含抗致病核苷酸序列，包括其启动子和由抗病原核酸序列编码的多肽。该组合物用于减少或消除由植物病原体引起的植物损伤的方法。还提供了具有增强的抗病性的转化植物，植物细胞，组织和种子。

95. 发明申请

WO0032761A8 ETHYLENE-RESPONSE-FACTOR1 (ERF1) IN PLANTS 审中-公开
标题翻译：乙烯响应因子1（ERF1）在植物中
公开(公告)号：WO0032761A8
公开(公告)日：2001-02-15
申请号：PCT/US9928104
申请日：1999-11-24
申请人： UNIV PENNSYLVANIA , ECKER JOSEPH R , SOLANO ROBERTO J
发明人： ECKER JOSEPH R , SOLANO ROBERTO J
IPC分类号： A01H5/00 , C07K14/415 , C07K16/16 , C12N5/10 , C12N15/09 , C12N15/82 , C12P21/08 , C12Q1/02 , C12N15/01 , A01H4/00 , C12N5/04 , C12N15/62 , C12P21/02
CPC分类号： C12N15/8238 , C07K14/415 , C12N15/8249 , C12N15/8266 , C12N15/8279
摘要： The invention provides an isolated nucleic acid encoding Ethylene Response Factor 1 (ERF1), an early responsive gene, encoding a GCC-box binding protein in the ethylene gas signaling pathway in plants. Also provided and characterized is the peptide ERF1, as well as methods of using ERF1 or its expression product to modulate the response in plants or plant cells to ethylene. Moreover, because ERF1 acts downstream of EIN3 and all previously identified members of the signaling pathway, and because constitutive expression of ERF1 results in the modulation of a variety of ethylene response genes and phenotypes, the invention provides novel and useful methods for regulating the ethylene signaling pathway.
摘要翻译：本发明提供编码乙烯应答因子1（ERF1）的分离的核酸，其是在植物中的乙烯气体信号传导途径中编码GCC-盒结合蛋白的早期响应基因。还提供并表征了肽ERF1，以及使用ERF1或其表达产物调节植物或植物细胞对乙烯的反应的方法。此外，因为ERF1在EIN3的下游和所有先前识别的信号通路成员中起作用，并且由于ERF1的组成型表达导致多种乙烯应答基因和表型的调节，本发明提供了用于调节乙烯信号传导的新颖和有用的方法途径。

96. 发明申请

WO00071732A2 NUCLEOTIDE SEQUENCE FOR INCREASING THE DEFENSE REACTION OF A PLANT AGAINST INFECTION BY A PATHOGEN 审中-公开
标题翻译：核苷酸增加防御反应植物病原体
公开(公告)号：WO00071732A2
公开(公告)日：2000-11-30
申请号：PCT/DE2000/001589
申请日：2000-05-19
IPC分类号： C07H21/00 , C12N15/29 , C12N15/82 , A01H5/00 , A01N65/00
CPC分类号： C07H21/00 , C12N15/8222 , C12N15/8237 , C12N15/8238 , C12N15/8239 , C12N15/8282
摘要： The invention relates to promoters which increase the pathogen-inducability of genes in plants, preferably in sugar beets, rape, potatoes, corn, soybeans, wheat, rice, rye, barley, cotton and sunflowers. The novel promoters are characterized in that they lead to the rapid local expression of the gene respectively under the control thereof in different organs and tissues of transgenic plants, such as leaves, shoots and/or roots, after having been infected by a pathogen or wounded. This attribute makes these sequences ideal for use as a regulation element for the controlled expression of antifungal compounds and thus for developing fungus-resistant plants.
摘要翻译：本发明涉及启动子增加基因的病原体诱导在植物中，优选在糖用甜菜，油籽油菜，马铃薯，玉米，大豆，小麦，稻，黑麦，大麦，棉花和向日葵。该新型的启动子由它们导致不同的器官和转基因植物的组织，例如叶，枝条和/或根，病原体或创伤的属性在其控制下的每个基因的快速局部表达的事实进一步区分。这种性质使得这些序列为抗真菌化合物的受控表达真菌具有抗性的植物的开发的理想调节元件，因此。

97. 发明申请

WO00032761A1 ETHYLENE-RESPONSE-FACTOR1 (ERF1) IN PLANTS 审中-公开
标题翻译：乙烯响应因子1（ERF1）在植物中
公开(公告)号：WO00032761A1
公开(公告)日：2000-06-08
申请号：PCT/US1999/028104
申请日：1999-11-24
IPC分类号： A01H5/00 , C07K14/415 , C07K16/16 , C12N5/10 , C12N15/09 , C12N15/82 , C12P21/08 , C12Q1/02 , C12N15/01 , A01H4/00 , C12N5/04 , C12N15/62 , C12P21/02
CPC分类号： C12N15/8238 , C07K14/415 , C12N15/8249 , C12N15/8266 , C12N15/8279
摘要： The invention provides an isolated nucleic acid encoding Ethylene Response Factor 1 (ERF1), an early responsive gene, encoding a GCC-box binding protein in the ethylene gas signaling pathway in plants. Also provided and characterized is the peptide ERF1, as well as methods of using ERF1 or its expression product to modulate the response in plants or plant cells to ethylene. Moreover, because ERF1 acts downstream of EIN3 and all previously identified members of the signaling pathway, and because constitutive expression of ERF1 results in the modulation of a variety of ethylene response genes and phenotypes, the invention provides novel and useful methods for regulating the ethylene signaling pathway.
摘要翻译：本发明提供编码乙烯应答因子1（ERF1）的分离的核酸，其是在植物中的乙烯气体信号传导途径中编码GCC-盒结合蛋白的早期响应基因。还提供和表征的是肽ERF1，以及使用ERF1或其表达产物调节植物或植物细胞对乙烯的响应的方法。此外，因为ERF1在EIN3的下游和所有先前识别的信号通路成员中起作用，并且由于ERF1的组成型表达导致各种乙烯应答基因和表型的调节，本发明提供了用于调节乙烯信号传导的新颖和有用的方法途径。

98. 发明申请

WO99064562A3 EXPRESSION CONTROL ELEMENTS FROM GENES ENCODING STARCH BRANCHING ENZYMES 审中-公开
标题翻译：来自基因的表达控制元件编码淀粉分支酶
公开(公告)号：WO99064562A3
公开(公告)日：2000-05-18
申请号：PCT/US1999/013266
申请日：1999-06-11
IPC分类号： C12N9/10 , C12N15/82 , C07H21/02 , C07K1/00 , C12N5/04 , C12N15/29
CPC分类号： C12N9/107 , C12N15/8234 , C12N15/8237 , C12N15/8238
摘要： Nucleic acid molecules for directing and modulating gene expression in transformed cells are disclosed. The molecules comprise 5' and 3' regulatory sequences isolated from genomic clones having homology in their coding regions with maize genes encoding Type I and Type IIB starch branching enzymes. Chimeric genes comprising these expression controlling nucleic acid molecules, and cells transformed with such chimeric genes, are also disclosed.
摘要翻译：公开了用于引导和调节转化细胞中基因表达的核酸分子。分子包含从其编码区与编码I型和IIB型淀粉分支酶的玉米基因的同源性的基因组克隆分离的5'和3'调节序列。还公开了包含这些表达控制核酸分子的嵌合基因和用这种嵌合基因转化的细胞。

99. 发明申请

WO00018926A1 USE OF BIFUNCTIONAL alpha -AMYLASE SUBTILISIN INHIBITOR PROMOTER SEQUENCE OF BARLEY TO CONFER EXPRESSION IN SEEDS 审中-公开
标题翻译：使用双功能的α-亚磺酸酶抑制剂抑制剂启动子序列进行种子表达
公开(公告)号：WO00018926A1
公开(公告)日：2000-04-06
申请号：PCT/AU1999/000823
申请日：1999-09-24
IPC分类号： C07K14/415 , C07K14/81 , C12N15/29 , C12N15/82 , A01H1/00 , A01H5/10
CPC分类号： C12N15/8238 , C07K14/415 , C07K14/811 , C12N15/8234
摘要： The present invention provides a novel promoter that is functional in the seeds of plants, in particular the endosperm and/or aleurone and/or the scutellum. Preferably, the isolated promoter sequence of the invention further modulates the expression of a structural gene in response to the phytohormones ABA and/or GA. Alternatively or in addition, the promoter sequence of the present invention is capable of being repressed or otherwise down-regulated in response to GA. The present invention further encompasses genetic constructs capable of expressing a structural gene operably under the control of the inventive promoter sequence, and transgenic plants carrying the genetic constructs. The promoter sequence of the present invention is particularly useful for modifying the seed traits of plants.
摘要翻译：本发明提供了一种在植物种子，特别是胚乳和/或糊粉和/或盾片中起作用的新型启动子。优选地，本发明的分离的启动子序列进一步调节响应于植物激素ABA和/或GA的结构基因的表达。或者或另外，本发明的启动子序列能够响应于GA被抑制或以其它方式下调。本发明还包括能够在本发明启动子序列控制下可操作地表达结构基因的遗传构建体和携带遗传构建体的转基因植物。本发明的启动子序列特别可用于改变植物的种子性状。

100. 发明申请

WO9938988A3 A CHEMICAL INDUCIBLE PROMOTER USED TO OBTAIN TRANSGENIC PLANTS WITH A SILENT MARKER 审中-公开
标题翻译：用于获得无机标记的化学诱导剂用于获得转基因植物
公开(公告)号：WO9938988A3
公开(公告)日：1999-10-21
申请号：PCT/US9901629
申请日：1999-01-27
申请人： UNIV ROCKEFELLER
发明人： CHUA NAM-HAI , AOYAMA TAKASHI
IPC分类号： A01H1/00 , C12N5/10 , C12N15/09 , C12N15/82 , A01H5/00
CPC分类号： C12N15/8242 , C12N15/8209 , C12N15/8238
摘要： A chemically inducible promoter is described which may be used to transform plants with genes which are easily regulatable by adding plants or plant cells to a medium containing an inducer of the promoter or by removing the plants or plant cells from such medium. The promoter described is one which is inducible by a glucocorticoid which is not endogenous to plants. Such promoters may be used with a variety of genes such as ipt or knottedl to induce shoot formation is the presence of a glucocorticoid. The promoter may also be used with antibiotic or herbicide resistance genes which are then regulatable by the presence or absence of inducer rather than being constitutive. Other examples of genes which may be placed under the control of the inducible promoter are also presented.
摘要翻译：描述了一种化学诱导型启动子，其可用于通过将植物或植物细胞添加到含有启动子诱导剂的培养基的培养基中或通过从该培养基中除去植物或植物细胞而使易于调节的基因转化植物。描述的启动子是由植物不是内源性的糖皮质激素诱导的。这样的启动子可以与各种基因如ipt或knotted1一起使用以诱导芽形成是糖皮质激素的存在。启动子也可以与抗生素或除草剂抗性基因一起使用，然后通过存在或不存在诱导剂而不是组成型的基因。还可以列举可置于诱导型启动子控制下的基因的其它实例。

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