专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2004007752A2 SYSTEM AND METHOD FOR DETERMINING KNOWN DNA VARIANTS WITH TEMPERATURE GRADIENT ELECTROPHORESIS 审中-公开
标题翻译：用温度梯度电泳法测定已知DNA变异体的系统和方法
公开(公告)号：WO2004007752A2
公开(公告)日：2004-01-22
申请号：PCT/US0322067
申请日：2003-07-15
申请人： SPECTRUMEDIX LLC
发明人： LIU ZHAOWEI , GUO ZHIYONG , MAYE CHRISTINA , GUTSHALL KEVIN R
IPC分类号： C12Q1/68 , G01N27/447 , C12Q
CPC分类号： G01N27/44704 , C12Q1/6827 , C12Q2565/131 , C12Q2527/107
摘要： A first aspect of the invention relates to a method of determining the genotype of a sample polynucleotide having at least a first variant site. The method may include amplifying at least a portion of the sample polynucleotide to obtain first amplicons, the first amplicons including the first variant site. The position of the first variant site along the sample nucleotide is preferably known. The first amplicons may be combined with first and second different polynucleotide controls. The first and second polynucleotide controls may differ by at least one base therealong, the position of the at least one differing base preferably corresponds to the position of the first variant site along the sample polynucleotide. A plurality of first duplexes may be prepared. At least some and preferably each of at least some of the first duplexes may include (i) a polynucleotide strand of one of the first amplicons and (ii) a complementary polynucleotide strand of the first polynucleotide control. A plurality of second duplexes may be prepared. At least some and preferably each of at least some of the second duplexes including (i) a polynucleotide strand of one of the first amplicons and (ii) a complementary polynucleotide strand of the second polynucleotide control. The first and second duplexes may be subjected to temperature gradient electrophoresis (TGE) to obtain first and second electrophoresis data, which are indicative of the genotype of the first variant site. The genotype of the first variant site of the sample polynucleotide may be determined based on the first and second electrophoresis data. The determination of the genotype may include determining a number of peaks present in the first electrophoresis data and a number of peaks present in the second electrophoresis data.
摘要翻译：本发明的第一方面涉及确定具有至少第一变体位点的样品多核苷酸的基因型的方法。该方法可以包括扩增样品多核苷酸的至少一部分以获得第一扩增子，第一扩增子包括第一变体位点。沿着样品核苷酸的第一个变体位点的位置是优选的。第一个扩增子可以与第一个和第二个不同的多核苷酸对照组合。第一和第二个多核苷酸对照可以通过至少一个碱基不同，至少一个不同碱基的位置优选对应于沿着样品多核苷酸的第一个变体位点的位置。可以制备多个第一双链体。至少一些第一双链体中的至少一些优选各自可以包括（i）第一扩增子之一的多核苷酸链和（ii）第一多核苷酸对照的互补多核苷酸链。可以制备多个第二双链体。至少一些第二双链体的至少一些，优选各自包括（i）第一扩增子之一的多核苷酸链和（ii）第二多核苷酸对照的互补多核苷酸链。可以对第一和第二双链体进行温度梯度电泳（TGE）以获得指示第一变体位点的基因型的第一和第二电泳数据。可以基于第一和第二电泳数据确定样品多核苷酸的第一变体位点的基因型。基因型的确定可以包括确定存在于第一电泳数据中的多个峰和存在于第二电泳数据中的多个峰。

2. 发明申请

WO2004033621A3 SYSTEM AND METHOD FOR DETERMINING THE SIZES AND QUANTITY OF POLYNUCLEOTIDES WITH CAPILLARY ARRAY ELECTROPHORESIS 审中-公开
标题翻译：用毛细管阵列电泳法测定多晶硅的尺寸和数量的系统和方法
公开(公告)号：WO2004033621A3
公开(公告)日：2006-05-04
申请号：PCT/US0314389
申请日：2003-05-01
申请人： SPECTRUMEDIX LLC
发明人： LIU ZHAOWEI , GUO ZHIYONG , LI QINGBO , KANE THOMAS
IPC分类号： C12Q1/68 , G01N27/447 , G01N33/48 , C25B11/00 , C25B15/00
CPC分类号： G01N27/44782 , C12Q1/6816 , C12Q2565/125 , C12Q2545/113 , C12Q2545/101
摘要： An electrophoretic separation system configured to determine a size of each of a plurality of sample polynucleotides includes a plurality of sample separation lanes, such as capillaries. Each separation lane is configured to subject a number plurality of respective sample polynucleotides and a respective internal standard polynucleotide (ISP) to electrophoresis. The system also includes a ladder separation lane for subjecting at least two members of polynucleotide ladder to electrophoresis. A processor of the system is configured to determine migration coordinates of (1) the ISP and sample polynucleotides subjected to electrophoresis within each separation lane and (2) at least two of the PLMs. The processor also transforms the migration coordinates of the sample polynucleotides of each separation lane from a migration dimension of their respective separation lane to a migration dimension of the polynucleotide ladder. The sizes of the sample polynucleotides are determined based on (1) the respective transformed migration coordinates thereof and (2) migration coordinates of at least two PLMs.
摘要翻译：配置成确定多个样品多核苷酸中的每一个的尺寸的电泳分离系统包括多个样品分离通道，例如毛细管。每个分离通道被配置为使多个相应的多个样品多核苷酸和相应的内标多核苷酸（ISP）进行电泳。该系统还包括用于使多核苷酸梯度的至少两个成员进行电泳的梯子分离通道。该系统的处理器被配置为确定（1）在每个分离通道内经受电泳的ISP和样品多核苷酸的迁移坐标，以及（2）至少两个PLM。处理器还将每个分离通道的样品多核苷酸的迁移坐标从它们各自的分离通道的迁移维度转变为多核苷酸梯度的迁移维度。基于（1）各自的变换迁移坐标和（2）至少两个PLM的迁移坐标来确定样品多核苷酸的大小。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式