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    • 5. 发明申请
    • A NOVEL GROUP OF ESTERASES FOR THE ENANTIOSELECTIVE PRODUCTION OF FINE AND SPECIALITY CHEMICALS
    • 一种新的精选和特种化学品生产的新一代产品
    • WO2007128496A2
    • 2007-11-15
    • PCT/EP2007/003919
    • 2007-05-03
    • B.R.A.I.N. AGLIEBETON, KlausECK, JürgenBORNSCHEUER, UweBÖTTCHER, DominiqueLANGER, PeterBELLUR, Esen
    • LIEBETON, KlausECK, JürgenBORNSCHEUER, UweBÖTTCHER, DominiqueLANGER, PeterBELLUR, Esen
    • C12N9/18C12N15/55C07K16/40C12N5/10
    • C12N9/18
    • The present invention relates to a polynucleotide encoding an enzyme having carboxylesterase [E. C. 3.1.1.1] activity, wherein the coding sequence is selected from the group consisting of (a) a polynucleotide encoding an amino acid sequence as depicted in any one of SEQ ID NOs: 2, 4, 6, 8, 10 and 12; (b) a polynucleotide having or comprising a nucleotide sequence encoding an enzyme, wherein the nucleic acid sequence is as shown in any one of SEQ ID NOs: 1, 3, 5, 7, 9 and 11; (c) a polynucleotide having or comprising a nucleotide sequence encoding a fragment or derivative of an enzyme encoded by a polynucleotide of (a) or (b), wherein in said derivative one or more amino acid residues are conservatively substituted compared to the amino acid sequence of (a); (d) a polynucleotide encoding an enzyme having carboxylesterase activity which polynucleotide is at least 66% identical to a polynucleotide encoding an enzyme as shown in one of SEQ ID NOs: 2, 4, 6, 8, 10 and 12; (e) a polynucleotide having or comprising a nucleotide sequence the complementary strand of which hybridizes to a polynucleotide as defined in any one of (a) to (d); and (T) a polynucleotide having or comprising a nucleotide sequence being degenerate to the nucleotide sequence of the polynucleotide of (d) or (e); or the complementary strand of such a polynucleotide of (a) to (f) or fragments thereof useful as specific probes or primers. The present invention also relates to a host genetically engineered with the polynucleotide of the present invention or the vector of the present invention. The present invention also relates to a process for producing a polypeptide having carboxylesterase [E. C. 3.1.1.1] activity, comprising culturing the host of the present invention and recovering the polypeptide produced by said host. Moreover, The present invention also relates to a process for producing bacteria or eukaryotic cells capable of expressing a polypeptide having carboxylesterase [E. C. 3.1.1.1] activity, the process comprising genetically engineering bacteria or eukaryotic cells with the vector of the present invention. Finally, the present invention relates to (poly)peptides, antibodies, compositions and various methods for the production of optically active compounds.
    • 本发明涉及编码具有羧酸酯酶[E. 3.1.1.1]活性,其中编码序列选自(a)编码SEQ ID NO:2,4,6,8,10和12中任一个所示的氨基酸序列的多核苷酸 ; (b)具有或包含编码酶的核苷酸序列的多核苷酸,其中所述核酸序列如SEQ ID NO:1,3,5,7,9和11中任一个所示; (c)具有或包含编码由(a)或(b)的多核苷酸编码的酶的片段或衍生物的核苷酸序列的多核苷酸,其中在所述衍生物中,与氨基酸相比,一个或多个氨基酸残基被保守取代 (a)的顺序; (d)编码具有羧酸酯酶活性的多核苷酸的多核苷酸与编码如SEQ ID NO:2,4,6,8,10和12之一所示的酶的多核苷酸至少66%相同; (e)具有或包含核苷酸序列的多核苷酸,其互补链与(a)至(d)中任一项所定义的多核苷酸杂交; 和(T)具有或包含与(d)或(e)的多核苷酸的核苷酸序列退化的核苷酸序列的多核苷酸; 或(a)至(f)的这种多核苷酸或其片段的互补链用作特异性探针或引物。 本发明还涉及用本发明的多核苷酸或本发明的载体进行遗传工程改造的宿主。 本发明还涉及产生具有羧酸酯酶[E. 3.1.1.1]活性,包括培养本发明的宿主并回收由所述宿主产生的多肽。 此外,本发明还涉及能够表达具有羧酸酯酶[E]的多肽的细菌或真核细胞的生产方法。 3.1.1.1]活性,该方法包括用本发明的载体将细菌或真核细胞遗传工程化。 最后,本发明涉及(多)肽,抗体,组合物和用于制备光学活性化合物的各种方法。
    • 9. 发明申请
    • PROTEASE FOR WOUND CONDITIONING AND SKIN CARE
    • 蛋白酶对伤口调理和皮肤护理
    • WO2010099955A1
    • 2010-09-10
    • PCT/EP2010/001328
    • 2010-03-03
    • B.R.I.A.N. BIOTECHNOLOGY RESEARCH AND INFORMATION NETWORK AGNIEHAUS, FrankECK, JürgenSCHULZE, Renate
    • NIEHAUS, FrankECK, JürgenSCHULZE, Renate
    • C12N9/64C07K16/40A61K38/43C12N15/62C12N5/10
    • C12N9/6408
    • We have identified by molecular cloning a protease which originates from the larvae of Lucilia sericata and which was termed debrilase due to its activities useful for debridement of wounds. Described is a nucleic acid molecule encoding a serine protease having the ability to cleave fibrin and casein which is (a) a nucleic acid molecule encoding the serine protease comprising or consisting of the amino acid sequence of SEQ ID NO: 4 as well as to nucleic acid molecules encoding precursors or fragments of said serine protease; (b) a nucleic acid molecule comprising or consisting of the nucleotide sequence of SEQ ID NO: 3; (c) a nucleic acid molecule encoding a serine protease the amino acid sequence of which is at least 80 % identical to the amino acid sequence of (a), preferably at least 85 % identical, more preferably at least 90 % identical, and most preferred 95% identical; (d) a nucleic acid molecule comprising or consisting of a nucleotide sequence which is at least 80 % identical to the nucleotide sequence of (b), preferably at least 85 % identical, more preferably at least 90 % identical, and most preferred 95% identical; (e) a nucleic acid molecule which is degenerate with respect to the nucleic acid molecule of (b) or (d); or (f) a nucleic acid molecule corresponding to the nucleic acid molecule of any one of (a) to (d) wherein T is replaced by U.
    • 我们已经通过分子克隆鉴定了来源于丝光绿蝇幼虫的蛋白酶,并且由于其活性可用于伤口清创,因此被称为清创酶。 描述了编码丝氨酸蛋白酶的核酸分子,所述丝氨酸蛋白酶具有切割纤维蛋白和酪蛋白的能力,所述纤维蛋白和酪蛋白是(a)编码包含SEQ ID NO:4的氨基酸序列或由SEQ ID NO:4的氨基酸序列组成的丝氨酸蛋白酶的核酸分子, 编码所述丝氨酸蛋白酶的前体或片段的酸分子; (b)包含SEQ ID NO:3的核苷酸序列或由其组成的核酸分子; (c)编码丝氨酸蛋白酶的核酸分子,其氨基酸序列与(a)的氨基酸序列至少80%相同,优选至少85%相同,更优选至少90%相同,且大多数 优选的95%相同; (d)包含与(b)的核苷酸序列至少80%相同,优选至少85%相同,更优选至少90%相同,并且最优选95%相同的核苷酸序列或由其组成的核酸分子, 相同; (e)相对于(b)或(d)的核酸分子简并的核酸分子; 或(f)对应于(a)至(d)中任一项的核酸分子的核酸分子,其中T被U取代。