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1. 发明申请

WO2004068934A2 BREEDING PLANTS 审中-公开
标题翻译：种植植物
公开(公告)号：WO2004068934A2
公开(公告)日：2004-08-19
申请号：PCT/EP2004/050085
申请日：2004-02-05
申请人： CROPDESIGN N.V. , DILLEN, Willy , LEJEUNE, Pierre , BROEKAERT, Willem , LEYNS, Frederik
发明人： DILLEN, Willy , LEJEUNE, Pierre , BROEKAERT, Willem , LEYNS, Frederik
IPC分类号： A01G
CPC分类号： A01G9/143 , A01G31/042 , Y02A40/252 , Y02P60/216
摘要： A process for breeding plants which comprises growing plants of a species in an array of containers charged with growing medium of uniform characteristics in an environment of controlled climatic conditions with controlled supply of nutrients and feed water and changing the positions of the containers within the environment as required to ensure at least substantially uniform exposure of all plants in the containers to conditions in the environment. A process for the breeding of open pollinating plants in a greenhouse environment is also provided.
摘要翻译：一种育种植物的方法，其包括在受控的气候条件的环境中装载具有均匀特性的生长培养基的容器阵列中种植植物的植物，所述气候条件受控地供应营养物和给水以及将所述容器在所述环境中的位置改变为需要确保容器中所有植物至少基本均匀地暴露于环境条件。还提供了在温室环境中育种开放授粉植物的过程。

2. 发明申请

WO2006029987A1 ROOT EVALUATION 审中-公开
标题翻译： ROOT评估
公开(公告)号：WO2006029987A1
公开(公告)日：2006-03-23
申请号：PCT/EP2005/054466
申请日：2005-09-08
申请人： CROPDESIGN N.V. , LEJEUNE, Pierre , BROEKAERT, Willem , LEYNS, Frederik , DHONT, Frederik
发明人： LEJEUNE, Pierre , BROEKAERT, Willem , LEYNS, Frederik , DHONT, Frederik
IPC分类号： A01G9/00 , A01G31/00 , A01G1/00
CPC分类号： H04N7/002 , A01C1/00 , A01G2/00 , A01G9/00 , A01G31/00 , Y02P60/216
摘要： A method is provided comprising (i) growing a plant in a substantially transparent container charged with a particulate, non-transparent growing medium; and (ii) evaluating plant roots through said transparent container by digital imaging. An apparatus for evaluating plant roots in a high throughput manner is also provided.
摘要翻译：提供了一种方法，其包括（i）在装有微粒非透明生长介质的基本上透明的容器中生长植物; 和（ii）通过数字成像通过所述透明容器评估植物根。还提供了一种用于以高通量方式评估植物根的装置。

3. 发明申请

WO2002083901A2 PLANT GROWTH REGULATING GENES, PROTEINS AND USES THEREOF 审中-公开
标题翻译：植物生长调节基因，蛋白质及其用途
公开(公告)号：WO2002083901A2
公开(公告)日：2002-10-24
申请号：PCT/EP2002/004035
申请日：2002-04-11
申请人： CROPDESIGN N.V. , SAUTER, Margret , LORBIECKE, René , MIRONOV, Vladimir , FRANKARD, Valérie , DILLEN, Willy , LEJEUNE, Pierre , BROEKAERT, Willem
发明人： SAUTER, Margret , LORBIECKE, René , MIRONOV, Vladimir , FRANKARD, Valérie , DILLEN, Willy , LEJEUNE, Pierre , BROEKAERT, Willem
IPC分类号： C12N15/29
CPC分类号： C12N15/8262 , C07K14/415 , C12N15/8237 , C12N15/8261 , Y02A40/146
摘要： Provided are methods for controlling or altering the growth characteristics of a plant or of an organ or tissue or cell of this plant. This method comprises introduction and/or expression of one or more Growth Regulating Proteins functional in a plant or parts thereof and/or one or more DNA sequences encoding such proteins. Another method comprises contacting a plant or plant tissue with the purified Growth Regulated Protein or the active product derived from it. Provided are also DNA sequences encoding plant Growth Regulating Protein(s) as well as methods for obtaining the same. Furthermore, vectors comprising said DNA sequences are described, wherein the DNA sequences are operatively linked to regulatory elements allowing expression in prokaryotic and/or eukaryotic host cells. In addition, proteins encoded by said DNA sequences, antibodies to said proteins and methods for their production are provided. Furthermore, regulatory sequences, which naturally regulate the expression of the above-described DNA sequences, are described. Methods for the identification of compounds being capable of interacting with said Growth Regulating Proteins are described as well. Furthermore, transgenic plant cells, plant tissues and plants containing the above-described DNA sequences and vectors are described, as well as the use of the aforementioned DNA sequences, vectors, proteins, antibodies, regulatory sequences and/or compounds identified by the method of the invention in plant cell and tissue culture, plant breeding and/or agriculture.
摘要翻译：提供了用于控制或改变植物或该植物的器官或组织或细胞的生长特性的方法。该方法包括在植物或其部分和/或编码这种蛋白质的一个或多个DNA序列中引入和/或表达一种或多种功能的生长调节蛋白。另一种方法包括使植物或植物组织与纯化的生长调节蛋白或其衍生的活性产物接触。还提供编码植物生长调节蛋白的DNA序列以及获得其的方法。此外，描述了包含所述DNA序列的载体，其中所述DNA序列可操作地连接到允许在原核和/或真核宿主细胞中表达的调节元件。此外，提供了由所述DNA序列编码的蛋白质，对所述蛋白质的抗体及其生产方法。此外，描述了调节序列，其自然调节上述DNA序列的表达。还描述了用于鉴定能够与所述生长调节蛋白相互作用的化合物的方法。此外，描述了含有上述DNA序列和载体的转基因植物细胞，植物组织和植物，以及使用上述DNA序列，载体，蛋白质，抗体，调节序列和/或化合物本发明在植物细胞和组织培养，植物育种和/或农业中。

4. 发明申请

WO2003025185A1 A METHOD TO MODIFY CELL NUMBER, ARCHITECTURE AND YIELD OF PLANTS BY OVEREXPRESSING THE E2F TRANSCRIPTION FACTOR 审中-公开
标题翻译：通过过度表达E2F转录因子修饰植物细胞数，构建和产量的方法
公开(公告)号：WO2003025185A1
公开(公告)日：2003-03-27
申请号：PCT/EP2002/010236
申请日：2002-09-12
申请人： CROPDESIGN N.V. , BEECKMAN, Tom , DE VEYLDER, Lieven , INZE, Dirk , MIRONOV, Vladimir , BROEKAERT, Willem , DILLEN, Willy , FRANKARD, Valerie
发明人： BEECKMAN, Tom , DE VEYLDER, Lieven , INZE, Dirk , MIRONOV, Vladimir , BROEKAERT, Willem , DILLEN, Willy , FRANKARD, Valerie
IPC分类号： C12N15/82
CPC分类号： C12N15/8216 , C12N15/8261 , Y02A40/146
摘要： The present invention relates to a method for modifying plant growth and development processes by affecting cell cycle regulation comprising modulating expression of members of the plant E2F transcription factor family. More specific the invention relates to methods for stimulating cell division in a plant. The invention further relates to transgenic plants produced by the methods of the invention having altered plant growth and development characteristics compared to their isogenic counterparts. Preferably, the plant characteristics modified by the present invention include enlarged cotyledons, enhanced growth rate, stress resistance and seedling survival, more cells, more tillers, more panicles.
摘要翻译：本发明涉及通过影响细胞周期调节来修饰植物生长和发育过程的方法，其包括调节植物E2F转录因子家族成员的表达。更具体地，本发明涉及刺激植物细胞分裂的方法。本发明还涉及通过与其同基因对应物相比具有改变的植物生长和发育特征的本发明方法生产的转基因植物。优选地，通过本发明修饰的植物特征包括扩大的子叶，增强的生长速率，抗胁迫性和幼苗存活，更多的细胞，更多的分蘖，更多的穗。

5. 发明申请

WO2005024029A2 PLANTS HAVING MODIFIED GROWTH CHARACTERISTICS AND METHOD FOR MAKING THE SAME 审中-公开
标题翻译：具有改良增长特性的植物及其制造方法
公开(公告)号：WO2005024029A2
公开(公告)日：2005-03-17
申请号：PCT/EP2004/052035
申请日：2004-09-03
申请人： CROPDESIGN N.V. , BROEKAERT, Willem , FRANKARD, Valerie , HATZFELD, Yves , MIRONOV, Vladimir
发明人： BROEKAERT, Willem , FRANKARD, Valerie , HATZFELD, Yves , MIRONOV, Vladimir
IPC分类号： C12N15/82
CPC分类号： C12N9/1205 , C12N15/8262 , C12Q1/485 , G01N2333/39 , G01N2500/10
摘要： The present invention concerns a method for modifying the growth characteristics of plants by modulating expression in a plant of a B-type CDK nucleic acid and/or modulating activity and/or levels in a plant of a B-type CDK protein. The invention also relates to transgenic plants having modified growth characteristics, which plants have modulated expression of a B-type CDK nucleic acid and/or modulated activity and/or levels in a plant of a B-type CDK protein relative to expression, activity and/or levels in corresponding wild-type plants. The present invention also provides a novel screening method for the identification of mutant CDKs having enhanced CDK activity relative to corresponding non-mutated CDKs. The present invention also provides a novel screening method for the identification of non-active CDKs that are able to bind to CKIs (cyclin dependent kinase inhibitors). The invention also provides mutant CDKs obtainable by the screening methods according to the invention.
摘要翻译：本发明涉及通过调节植物中B型CDK核酸的表达和/或调节植物中B的活性和/或水平来修饰植物生长特性的方法型CDK蛋白。本发明还涉及具有修饰的生长特征的转基因植物，所述植物调节B型CDK核酸的表达和/或调节B型CDK蛋白的植物中的活性和/或水平相对于表达，活性和 /或相应野生型植物中的水平。本发明还提供了用于鉴定相对于相应的未突变的CDK具有增强的CDK活性的突变型CDK的新型筛选方法。本发明还提供了用于鉴定能够结合CKI（细胞周期蛋白依赖性激酶抑制剂）的非活性CDK的新型筛选方法。本发明还提供了通过根据本发明的筛选方法可获得的突变型CDK。

6. 发明申请

WO2003060134A1 HERBICIDE MODE OF ACTION DETERMINATION 审中-公开
标题翻译：除草剂作用方式决定
公开(公告)号：WO2003060134A1
公开(公告)日：2003-07-24
申请号：PCT/EP2003/000512
申请日：2003-01-14
申请人： CROPDESIGN N.V. , BROEKAERT, Willem , INZE, Dirk
发明人： BROEKAERT, Willem , INZE, Dirk
IPC分类号： C12N15/82
CPC分类号： C12N15/8274 , G01N33/5097
摘要： The present invention relates to methods to determine the molecular target of an agrochemical or candidate agrochemical compound. The methods of the present invention can also be used as screening assay to identify new agrochemicals with pesticidal potency or with growth regulatory potency (such as herbicides or growth stimulators). The invention further relates to agrochemical and agrochemical compositions and methods for preparing these. The invention further provides plants that are tolerant to an agrochemcial compound.
摘要翻译：本发明涉及确定农用化学品或候选农药化合物的分子靶标的方法。本发明的方法也可用作筛选测定以鉴定具有杀虫潜力或具有生长调节效力的新农药（如除草剂或生长刺激剂）。本发明还涉及农用化学品和农药组合物及其制备方法。本发明还提供了耐农药化合物的植物。

7. 发明申请

WO2003085115A2 IDENTIFICATION AND VALIDATION OF NOVEL TARGETS FOR AGROCHEMICALS 审中-公开
标题翻译：鉴定和验证农业新型目标
公开(公告)号：WO2003085115A2
公开(公告)日：2003-10-16
申请号：PCT/EP2003/003703
申请日：2003-04-08
申请人： CROPDESIGN N.V. , INZE, Dirk , BROEKAERT, Willem
发明人： INZE, Dirk , BROEKAERT, Willem
IPC分类号： C12N15/82
CPC分类号： C12N15/8261 , C12N15/8274 , C12Q1/6895 , C12Q2600/13 , C12Q2600/158 , Y02A40/146
摘要： The invention relates to a method for identifying and validating plant targets for agrochemicals, comprising the steps of determining gene or protein expression profiles in function of the progression of an essential biological process in a plant, and the subsequent downregulation of expression of said gene or protein in a plant cell. More particularly, the effects of downregulation of the candidate target gene were directly monitored on plants locally infected with a vector mediating viral induced gene suppression in that infected plant area. The invention also relates to isolated plant genes encoding proteins involved in plant growth and development. The invention also relates to plants tolerant to agrochemicals such as herbicides or pesticides.
摘要翻译：本发明涉及用于鉴定和验证农用化学品的植物靶标的方法，其包括以下步骤：确定基因或蛋白质表达谱，其功能是植物中基本生物学过程的进展，随后下调所述基因或蛋白质的表达在植物细胞中。更具体地说，候选靶基因的下调效应直接由在感染的植物区域中介导病毒诱导的基因抑制的载体局部感染的植物上监测。本发明还涉及编码参与植物生长和发育的蛋白质的分离的植物基因。本发明还涉及耐农业化学品如除草剂或杀虫剂的植物。

8. 发明申请

WO2004076616A3 ARABIDOPSIS PROMOTERS 审中-公开
公开(公告)号：WO2004076616A3
公开(公告)日：2004-09-10
申请号：PCT/EP2004/050213
申请日：2004-02-26
申请人： CROPDESIGN N.V. , BROEKAERT, Willem , HATZFELD, Yves
发明人： BROEKAERT, Willem , HATZFELD, Yves
IPC分类号： C12N15/82
摘要： The present invention provides several promoters isolated from Arabidopsis thaliana, which promoters are capable of driving and/or regulating the expression of an operably linked nucleic acid in a plant. The expression pattern s of the promoters according to the present invention have been studied in Arabidopsis thaliana and some of the promoters displayed specific activity in particular cells, tissues or organs of the plant, while others displayed constitutive expression throughout substantially the whole plant. Some promoters showed weak expression, while others were strongly active.

9. 发明申请

WO2004070039A3 RICE PROMOTERS 审中-公开
公开(公告)号：WO2004070039A3
公开(公告)日：2004-08-19
申请号：PCT/EP2004/050081
申请日：2004-02-04
申请人： CROPDESIGN N.V. , HATZFELD, Yves , BROEKAERT, Willem
发明人： HATZFELD, Yves , BROEKAERT, Willem
IPC分类号： C12N15/82
摘要： The present invention provides several promoters isolated from Oryza sativa , which promoters are capable of driving and/or regulating the expression of a n operably linked nucleic acid in a plant. The expression pattern s of the promoters according to the present invention have been studied in Oryza sativa and some of the promoters displayed specific activity in particular cells, tissues or organs of the plant, while others displayed constitutive expression throughout substantially the whole plant. Some promoters showed weak expression, while others were strongly active.

10. 发明申请

WO2003085115A3 IDENTIFICATION AND VALIDATION OF NOVEL TARGETS FOR AGROCHEMICALS 审中-公开
公开(公告)号：WO2003085115A3
公开(公告)日：2003-10-16
申请号：PCT/EP2003/003703
申请日：2003-04-08
申请人： CROPDESIGN N.V. , INZE, Dirk , BROEKAERT, Willem
发明人： INZE, Dirk , BROEKAERT, Willem
IPC分类号： C12N15/82
摘要： The invention relates to a method for identifying and validating plant targets for agrochemicals, comprising the steps of determining gene or protein expression profiles in function of the progression of an essential biological process in a plant, and the subsequent downregulation of expression of said gene or protein in a plant cell. More particularly, the effects of downregulation of the candidate target gene were directly monitored on plants locally infected with a vector mediating viral induced gene suppression in that infected plant area. The invention also relates to isolated plant genes encoding proteins involved in plant growth and development. The invention also relates to plants tolerant to agrochemicals such as herbicides or pesticides.

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