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1. 发明申请

WO2010021702A1 SELF-AVOIDING MOLECULAR RECOGNITION SYSTEMS IN DNA AMPLIFICATION 审中-公开
标题翻译：在DNA扩增中的自我避免分子识别系统
公开(公告)号：WO2010021702A1
公开(公告)日：2010-02-25
申请号：PCT/US2009004718
申请日：2009-08-19
申请人： BENNER STEVEN ALBERT , HOSHIKA SHUICHI , CHEN FEI
发明人： BENNER STEVEN ALBERT , HOSHIKA SHUICHI , CHEN FEI
IPC分类号： C07H21/04 , A61K31/70 , C12P19/34
CPC分类号： C12Q1/6853 , C07H21/04 , C12Q1/6848 , C12Q1/6876 , C12Q2525/117 , C12Q2600/16 , C12Q2531/113 , C12Q2527/107 , C12Q2525/185 , C12Q2537/143 , C12Q2525/101
摘要： This invention concerns self-avoiding molecular recognition systems (SAMRS), DNA-like molecules that that bind to natural DNA having a Watson-Crick complementary sequence, but do not bind to other SAMRS-containing oligonucleotide analogs when SAMRS components are paired with other SAMRS components and, where once bound, serve as primers for polymerase extension, as well as templates in reverse priming characteristic of the polymerase chain reaction. As a product of extensive experimentation, SAMRS building blocks are disclosed that confer these properties on oligonucleotides that contain them, positions in primers where SAMRS building blocks are preferably placed, and a critical mass of biophysical data to support heuristic rules to permit design of SAMRS component-containing sequences that can be used as primers in primer extension and PCR reactions. Also disclosed are compositions containing a plurality of these oligonucleotides that can be used in kits, and processes using these compositions to PCR amplify target oligonucleotides.
摘要翻译：本发明涉及自回避分子识别系统（SAMRS），当SAMRS组分与其他SAMRS配对时，结合具有沃森 - 克里克互补序列但不与其它含SAMRS的寡核苷酸类似物结合的天然DNA的DNA样分子组分，并且其中一旦结合，用作聚合酶延伸的引物，以及聚合酶链反应的反引发特征的模板。作为广泛实验的产物，公开了SAMRS构建单元，其赋予其含有它们的寡核苷酸的这些性质，优选放置SAMRS构建块的引物中的位置，以及关键质量的生物物理数据以支持启发式规则以允许设计SAMRS组分可用作引物延伸和PCR反应中的引物。还公开了含有可用于试剂盒中的多种这些寡核苷酸的组合物，以及使用这些组合物PCR扩增靶寡核苷酸的方法。

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