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1. 发明申请

US20080293109A1 Methods for degrading or converting plant cell wall polysaccharides 审中-公开
公开(公告)号：US20080293109A1
公开(公告)日：2008-11-27
申请号：US12172852
申请日：2008-07-14
申请人： Randy Berka , Joel Cherry
发明人： Randy Berka , Joel Cherry
IPC分类号： C12P13/24 , C12P1/00 , C12P7/04 , C12P7/00 , C12P13/04 , C12P7/16 , C12P7/10 , C12P13/06 , C12P13/08 , C12P13/10 , C12P13/12 , C12P13/14 , C12P13/16 , C12P13/18 , C12P13/20
CPC分类号： C12P19/14 , C12P5/023 , C12P7/00 , C12P7/02 , C12P7/04 , C12P7/06 , C12P7/10 , C12P7/16 , C12P7/18 , C12P7/20 , C12P7/26 , C12P7/28 , C12P7/40 , C12P13/04 , C12P17/04 , C12P19/02 , Y02E50/10 , Y02E50/16 , Y02E50/17 , Y02E50/343
摘要： The present invention relates to methods for converting plant cell wall polysaccharides into one or more products, comprising: treating the plant cell wall polysaccharides with an effective amount of a spent whole fermentation broth of a recombinant microorganism, wherein the recombinant microorganism expresses one or more heterologous genes encoding enzymes which degrade or convert the plant cell wall polysaccharides into the one or more products. The present invention also relates to methods for producing an organic substance, comprising: (a) saccharifying plant cell wall polysaccharides with an effective amount of a spent whole fermentation broth of a recombinant microorganism, wherein the recombinant microorganism expresses one or more heterologous genes encoding enzymes which degrade or convert the plant cell wall polysaccharides into saccharified material; (b) fermenting the saccharified material of step (a) with one or more fermenting microoganisms; and (c) recovering the organic substance from the fermentation.

2. 发明授权

US6083728A Production of glutamate using wild type Bacillus methanolicus 失效
标题翻译：使用野生型甲醇芽孢杆菌生产谷氨酸
公开(公告)号：US6083728A
公开(公告)日：2000-07-04
申请号：US953265
申请日：1997-10-17
申请人： Frederick J. Schendel , Richard Dillingham , Richard S. Hanson , Konosuke Sano , Kazuhiko Matsui
发明人： Frederick J. Schendel , Richard Dillingham , Richard S. Hanson , Konosuke Sano , Kazuhiko Matsui
IPC分类号： C12P13/14 , C12P13/18 , C12N1/20
CPC分类号： C12P13/18 , C12P13/14 , Y10S435/832
摘要： A method of producing glutamic acid by culturing a biologically pure wild type Bacillus methanolicus which exhibits sustained growth at 50.degree. C. using methanol as a carbon and energy source and requiring vitamin B.sub.12 and biotin is provided.
摘要翻译：通过培养生物纯的野生型甲醇甲醇生产谷氨酸的方法，其在50℃下使用甲醇作为碳和能量源并需要维生素B12和生物素显示出持续的生长。

3. 发明授权

US5492818A Method of producing L-glutamic acid by fermentation 失效
标题翻译：通过发酵生产L-谷氨酸的方法
公开(公告)号：US5492818A
公开(公告)日：1996-02-20
申请号：US354014
申请日：1994-12-05
申请人： Hidetsugu Nakazawa , Hiroki Kawashima , Inao Oyama , Keiji Ishii , Yoshio Kawahara
发明人： Hidetsugu Nakazawa , Hiroki Kawashima , Inao Oyama , Keiji Ishii , Yoshio Kawahara
IPC分类号： C12P13/14 , C12R1/13 , C12R1/15 , C12P13/18
CPC分类号： C12P13/14 , Y10S435/843
摘要： The present invention provides a method of producing L-glutamic acid by fermentation, comprising the steps ofculturing a mutant of an L-glutamic acid-producing microorganism of the genus Brevibacterium or Corynebacterium which has lower .alpha.-ketoglutaric acid dehydrogenase activity compared with the wild strains from which said mutant is derived, in a liquid nutrient culture medium containing biotin at a concentration of 10 to 1000 .mu.g/l without adding a biotin activity-suppressing substance thereto;producing and accumulating L-glutamic acid in the culture solution; andrecovering L-glutamic acid from said culture solution.According to the method of the present invention, it is possible to industrially produce L-glutamic acid by fermentation in a more economical and efficient manner.
摘要翻译：本发明提供通过发酵生产L-谷氨酸的方法，包括以下步骤：与野生型相比，培养具有较低α-酮戊二酸脱氢酶活性的短杆菌属或棒状杆菌属的L-谷氨酸生产微生物的突变体在不含生物素活性抑制物质的情况下，在含有浓度为10〜1000μg/ l的生物素的液体营养培养基中，所述突变体得自其中的菌株; 在培养液中产生和积累L-谷氨酸; 从所述培养液中回收L-谷氨酸。根据本发明的方法，可以以更经济和有效的方式通过发酵工业生产L-谷氨酸。

4. 发明授权

US4728610A Method for producing L-glutamic acid 失效
标题翻译： L-谷氨酸的制备方法
公开(公告)号：US4728610A
公开(公告)日：1988-03-01
申请号：US924141
申请日：1986-10-27
申请人： Yukihiro Kanegae , Yoshio Sugiyama , Isamu Nakatsui
发明人： Yukihiro Kanegae , Yoshio Sugiyama , Isamu Nakatsui
IPC分类号： C12P13/18 , C12P13/14 , C12R1/13 , C12R1/15 , C12P13/24
CPC分类号： C12P13/14 , Y10S435/84 , Y10S435/843
摘要： L-Glutamic acid is produced in a high yield by cultivating an L-glutamic acid-producing microorganism which requires oleic acid but does not require biotin for growth in a culture medium containing an oleic acid compound and a biotin compound of no less than 100 .mu.g/liter as biotin, with carbohydrate and acetic acid as carbon sources being maintained in a weight ratio of about 80:20 through about 40:60.
摘要翻译：通过培养需要油酸但不需要生物素来生长的L-谷氨酸微生物在含有油酸化合物和生物素化合物的培养基中培养不低于100μl的L-谷氨酸 g / L作为生物素，碳水化合物和乙酸作为碳源以约80:20至约40:60的重量比保持。

5. 发明授权

US10011839B2 Metabolic transistor in bacteria 有权
公开(公告)号：US10011839B2
公开(公告)日：2018-07-03
申请号：US15263146
申请日：2016-09-12
申请人： William Marsh Rice University
发明人： Ka-Yiu San , George N. Bennett , Hui Wu
IPC分类号： C12N9/10 , C12N15/52 , C12N9/02 , C12N9/88 , C12N9/00 , C12P13/14 , C12P13/06 , C12P13/08 , C12P7/56 , C12N9/80 , C12P1/04 , C12P7/06 , C12P7/18 , C12P7/40 , C12P7/46 , C12P7/54 , C12P13/04 , C12P13/18 , C12P13/22
CPC分类号： C12N15/52 , C12N9/0008 , C12N9/001 , C12N9/0073 , C12N9/1085 , C12N9/80 , C12N9/88 , C12N9/93 , C12P1/04 , C12P7/065 , C12P7/18 , C12P7/40 , C12P7/46 , C12P7/54 , C12P7/56 , C12P13/04 , C12P13/06 , C12P13/08 , C12P13/14 , C12P13/18 , C12P13/222 , C12P13/225 , C12Y102/01025 , C12Y102/04004 , C12Y102/07007 , C12Y103/01076 , C12Y103/05005 , C12Y103/99031 , C12Y114/13027 , C12Y205/01001 , C12Y205/01029 , C12Y205/01032 , C12Y205/01093 , C12Y305/01022 , C12Y305/99002 , C12Y401/01024 , C12Y499/01004 , C12Y602/01011 , Y02E50/17
摘要： The disclosure relates to a metabolic transistor in microbes such as bacteria and yeast where a competitive pathway is introduced to compete with a product pathway for available carbon so as to control the carbon flux in the microbe.

6. 发明授权

US09441253B2 Metabolic transistor in bacteria 有权
标题翻译：细菌中的代谢晶体管
公开(公告)号：US09441253B2
公开(公告)日：2016-09-13
申请号：US14176008
申请日：2014-02-07
申请人： William Marsh Rice University
发明人： Ka-Yiu San , George N. Bennett , Hui Wu
IPC分类号： C12P13/22 , C12P7/56 , C12P7/54 , C12P13/18 , C12P13/08 , C12P13/06 , C12N15/52 , C12N9/02 , C12N9/10 , C12N9/80 , C12N9/88 , C12N9/00 , C12P1/04 , C12P7/06 , C12P7/18 , C12P7/40 , C12P7/46 , C12P13/04 , C12P13/14
CPC分类号： C12P13/225 , C12N9/0008 , C12N9/001 , C12N9/0073 , C12N9/1085 , C12N9/80 , C12N9/88 , C12N9/93 , C12N15/52 , C12P1/04 , C12P7/065 , C12P7/18 , C12P7/40 , C12P7/46 , C12P7/54 , C12P7/56 , C12P13/04 , C12P13/06 , C12P13/08 , C12P13/14 , C12P13/18 , C12P13/222 , C12Y102/01025 , C12Y102/04004 , C12Y102/07007 , C12Y103/01076 , C12Y103/05005 , C12Y114/13027 , C12Y205/01001 , C12Y205/01032 , C12Y205/01093 , C12Y305/01022 , C12Y305/99002 , C12Y401/01024 , C12Y602/01011 , Y02E50/17 , Y02P20/52
摘要： The disclosure relates to a metabolic transistor in bacteria where a competitive pathway is introduced to compete with a product pathway for available carbon so as to control the carbon flux in the bacteria.
摘要翻译：本公开涉及细菌中的代谢晶体管，其中引入竞争性途径以与可用碳的产物途径竞争，以便控制细菌中的碳通量。

7. 发明授权

US06165731A Assay for the ubiquitination-promoting activity of human proteins 失效
标题翻译：测定人类蛋白质泛素化促进活性
公开(公告)号：US06165731A
公开(公告)日：2000-12-26
申请号：US235572
申请日：1999-01-22
申请人： Raymond Deshaies , Svetlana Lyapina , Craig C. Correll
发明人： Raymond Deshaies , Svetlana Lyapina , Craig C. Correll
IPC分类号： C07K16/14 , C07K16/18 , C12Q1/25 , G01N33/53 , A61K39/395 , C07K16/00 , C12P13/18 , G01N33/573
CPC分类号： C07K16/18 , C07K16/14 , C12Q1/25 , G01N2333/9015 , G01N2500/00
摘要： A method is provided for identifying an compound that affects an activity of a polypeptide subunit of a SCF complex. The method includes contacting a sample comprising a chimeric SCF complex assembled from subunits derived from Saccharomyces cerevisiae or human and another species and a CDC34p polypeptide with the compound under conditions that allow the components to interact, and adding to these components an E1 enzyme, ubiquitin and ATP, and a SCF substrate. The ubiquitination of the SCF substrate is measured. A chimeric in vitro assay system is provided for measuring CDC53p or CUL1p activity, comprising a CDC4p, CDC34p, and a SKP1p polypeptide, and either a CDC53p or CUL1p polypeptide. In this assay the CDC4p, CDC34p, and SKP1p polypeptide are either a yeast polypeptide or a polypeptide from another species, and at least one of the CDC4p, CDC34p, and SKP1p polypeptides is a yeast polypeptide and at least one of the CDC4p, CDC34p, and SKP1p polypeptides is a polypeptide from another species. A method is further provided for identifying a compound that affects the ability of a CDC4p, a SKP1p, a CDC34p, and a CDC53p or a CUL1p to ubiquitinate a substrate. The method includes contacting a sample comprising a CDC4p, a SKP1p, a CDC34p, and a CDC53p or CUL1p, with the compound under conditions sufficient to allow the components to interact, and adding to these components an E1 enzyme, ubiquitin and ATP, and a substrate for ubiquitination. The ability of the CDC4p, the SKP1p, the CDC34p, and the CDC53p or CUL1p, to ubiquitinate the substrate is measured. A method is also provided of identifying a polypeptide having a function of a CDC4 subunit of SCF. A method is further provided for identifying a polypeptide as a substrate for a ubiquitination reaction.
摘要翻译：提供了用于鉴定影响SCF复合物的多肽亚基的活性的化合物的方法。该方法包括在允许组分相互作用的条件下将包含由来自酿酒酵母（Saccharomyces cerevisiae）或人与另一物种的亚基组装的样品与CDC34p多肽与所述化合物接触，并将E1酶，泛素和 ATP和SCF底物。测量SCF底物的泛素化。提供嵌合体外测定系统用于测量CDC53p或CUL1p活性，其包含CDC4p，CDC34p和SKP1p多肽，以及CDC53p或CUL1p多肽。在该测定中，CDC4p，CDC34p和SKP1p多肽是酵母多肽或来自另一物种的多肽，并且CDC4p，CDC34p和SKP1p多肽中的至少一个是酵母多肽，并且CDC4p，CDC34p，和SKP1p多肽是来自另一物种的多肽。还提供了一种鉴定影响CDC4p，SKP1p，CDC34p和CDC53p或CUL1p泛素化底物的能力的化合物的方法。该方法包括使包含CDC4p，SKP1p，CDC34p和CDC53p或CUL1p的样品与足以允许组分相互作用的条件下与化合物接触，并将E1酶，泛素和ATP以及底物用于泛素化。测量CDC4p，SKP1p，CDC34p和CDC53p或CUL1p的泛素化底物的能力。还提供了鉴定具有SCF的CDC4亚基的功能的多肽的方法。还提供了一种鉴定多肽作为泛素化反应底物的方法。

8. 发明授权

US5869300A Method for producing L-glutamic acid by continuous fermentation 失效
标题翻译：通过连续发酵生产L-谷氨酸的方法
公开(公告)号：US5869300A
公开(公告)日：1999-02-09
申请号：US974919
申请日：1997-11-20
申请人： Tatsuya Yoshioka , Toshimasa Ishii , Yoshio Kawahara , Yosuke Koyama , Eiko Shimizu
发明人： Tatsuya Yoshioka , Toshimasa Ishii , Yoshio Kawahara , Yosuke Koyama , Eiko Shimizu
IPC分类号： C12P13/14 , C12R1/13 , C12P13/18
CPC分类号： C12P13/14 , Y10S435/813 , Y10S435/84 , Y10S435/843
摘要： A method for producing L-glutamic acid, comprising inoculating a microorganism having an ability to produce L-glutamic acid, in a liquid medium containing a carbon source and a nitrogen source, conducting continuous L-glutamic acid fermentation in which both a carbon source and a nutrient having an effect of promoting bacterial growth are fed so as to make the microorganism grow, and then collecting L-glutamic acid produced and accumulated in a culture broth.
摘要翻译：一种生产L-谷氨酸的方法，包括在含有碳源和氮源的液体培养基中接种具有产生L-谷氨酸能力的微生物，进行连续的L-谷氨酸发酵，其中碳源和喂养具有促进细菌生长的作用的营养物，使微生物生长，然后收集培养液中产生并积累的L-谷氨酸。

9. 发明申请

US20070212764A1 METHOD FOR PRODUCING L-AMINO ACIDS USING BACTERIUM OF THE ENTEROBACTERIACEAE FAMILY 审中-公开
标题翻译：利用肠杆菌属家禽生产L-氨基酸的方法
公开(公告)号：US20070212764A1
公开(公告)日：2007-09-13
申请号：US11275507
申请日：2006-01-11
申请人： Leonid Ptitsyn , Irina Altman , Veronika Kotliarova , Yury Kozlov
发明人： Leonid Ptitsyn , Irina Altman , Veronika Kotliarova , Yury Kozlov
IPC分类号： C12P13/18 , C12P13/04 , C12P13/24 , C12P13/22 , C12P13/20 , C12P13/14 , C12P13/16 , C12P13/12 , C12P13/10 , C12N1/21
CPC分类号： C12P13/04
摘要： There is disclosed a method for producing an L-amino acid, for example L-threonine, L-lysine, L-histidine, L-phenylalanine, L-arginine, L-tryptophan, or L-glutamic acid, using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified to enhance an activity of N-acetylglucosamine permease encoded by the nagE gene.
摘要翻译：公开了使用细菌的L-氨基酸，例如L-苏氨酸，L-赖氨酸，L-组氨酸，L-苯丙氨酸，L-精氨酸，L-色氨酸或L-谷氨酸来制备L-氨基酸的方法肠杆菌科，其中细菌已被修饰以增强由nagE基因编码的N-乙酰葡糖胺通透酶的活性。

10. 发明申请

US20070077622A1 Corynebacterium glutamicum genes encoding proteins involved in carbon metabolism and energy production 审中-公开
标题翻译：编码参与碳代谢和能量产生的蛋白质的谷氨酸棒杆菌基因
公开(公告)号：US20070077622A1
公开(公告)日：2007-04-05
申请号：US11505198
申请日：2006-08-15
申请人： Markus Pompejus , Burkhard Kroger , Hartwig Schroder , Oskar Zelder , Gregor Haberhauer
发明人： Markus Pompejus , Burkhard Kroger , Hartwig Schroder , Oskar Zelder , Gregor Haberhauer
IPC分类号： C07H21/04 , C12P21/06 , C12P13/04 , C12P13/24 , C12P13/22 , C12P13/20 , C12P13/14 , C12P13/18 , C12P13/16 , C12P13/12 , C12P13/10 , C12P13/08 , C12N9/10 , C12N1/21 , C12N15/74
CPC分类号： C12N9/18 , C07K14/34 , C07K2319/00 , C12N9/00 , C12N9/90 , C12P7/18 , C12P7/40 , C12P7/6409 , C12P7/6427 , C12P7/6463 , C12P7/6472 , C12P13/04 , C12P13/06 , C12P13/08 , C12P13/10 , C12P13/12 , C12P13/14 , C12P13/20 , C12P13/222 , C12P13/225 , C12P13/227 , C12P13/24 , C12P19/00 , C12P19/34 , C12Y301/01031
摘要： Isolated nucleic acid molecules, designated SMP nucleic acid molecules, which encode novel SMP proteins from Corynebacterium glutamicum are described. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing SMP nucleic acid molecules, and host cells into which the expression vectors have been introduced. The invention still further provides isolated SMP proteins, mutated SMP proteins, fusion proteins, antigenic peptides and methods for the improvement of production of a desired compound from C. glutamicum based on genetic engineering of SMP genes in this organism.
摘要翻译：描述了编码来自谷氨酸棒杆菌的新型SMP蛋白质的分离的核酸分子，称为SMP核酸分子。本发明还提供了反义核酸分子，含有SMP核酸分子的重组表达载体，以及引入了表达载体的宿主细胞。本发明还基于该生物体中的SMP基因的遗传工程，进一步提供了分离的SMP蛋白，突变的SMP蛋白，融合蛋白，抗原肽和用于从谷氨酸棒球菌（C.GM）生产所需化合物的方法。

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