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1. 发明授权

US4205132A Lyophilization of bacteria 失效
标题翻译：细菌冻干
公开(公告)号：US4205132A
公开(公告)日：1980-05-27
申请号：US925034
申请日：1978-07-17
申请人： William E. Sandine , Ebenezer R. Vedamuthu
发明人： William E. Sandine , Ebenezer R. Vedamuthu
IPC分类号： A23C19/032 , C12N1/04 , C12K1/08 , A23C19/02 , C12K3/00
CPC分类号： C12N1/04 , A23C19/0323 , Y10S435/885
摘要： Storage stable, lyophilized, acid producing bacteria, such as lactic acid bacteria, are prepared by lyophilizing the bacteria in the presence of at least 5% by weight of the bacteria of a basic organic or inorganic buffering agent, preferably an alkali metal salt of glycerophosphate, to provide lyophilized bacteria containing less than 5% by weight water, and sealing the lyophilized bacteria in a container while in a vacuum or surrounded by a gaseous atmosphere substantially free of oxygen, preferably argon which is essentially free of oxygen.
摘要翻译：在至少5重量％的碱性有机或无机缓冲剂的细菌，优选甘油磷酸的碱金属盐的存在下，将细菌冻干，制备储存稳定的，冻干的酸产细菌如乳酸菌提供含有小于5重量％水的冻干细菌，并且在真空中将冻干细菌密封在容器中，或者由基本上不含氧的气体气氛（优选基本上不含氧的氩气）包围。

2. 发明授权

US3893308A Freezing apparatus particularly useful for freezing spermatozoa 失效
标题翻译：冷冻设备特别适用于冷冻精子
公开(公告)号：US3893308A
公开(公告)日：1975-07-08
申请号：US45078374
申请日：1974-03-13
申请人： RICOR LTD
发明人： BARKAY JOSEPH , ZUCKERMAN HENRYK , PUNDAK NACHMAN
IPC分类号： F25D3/10 , F25D29/00 , C12K1/08
CPC分类号： F25D29/001 , F25D3/10
摘要： Freezing apparatus is described particularly useful for producing frozen pellets of a medium containing spermatozoa, for use in artificial insemination. The apparatus includes a freezing chamber having a heat-conductive removable freezing plate constituting the top wall of a compartment into which a cooling gas is fed from a liquified gas container. A valve in the gas feed line controls the amount of gas fed to the freezing chamber to produce a preselected temperature as preset by a controller, and an electrical heating element within the liquified gas container effects the drive of the gas from the container to the freezing chamber. The heat-conductive plate is formed with a plurality of recessed pockets in which the medium is introduced and freezes in the form of pellets.
摘要翻译：描述冷冻装置特别适用于生产用于人工授精的含有精子的培养基的冷冻颗粒。该装置包括具有构成隔室的顶壁的导热性可移除冷冻板的冷冻室，冷却气体从液化气体容器供入冷冻室。气体供给管中的阀控制供给到冷冻室的气体量，以产生由控制器预设的预选温度，并且液化气体容器内的电加热元件影响气体从容器的驱动到冷冻房间。导热板形成有多个凹槽，其中介质被引入并以颗粒的形式冻结。

3. 发明授权

US07078224B1 Cell concentration and lysate clearance using paramagnetic particles 失效
公开(公告)号：US07078224B1
公开(公告)日：2006-07-18
申请号：US09475958
申请日：1999-12-30
申请人： Rex M. Bitner , Craig E. Smith , Douglas H. White , Braeden L. Butler , Jacqui Sankbeil
发明人： Rex M. Bitner , Craig E. Smith , Douglas H. White , Braeden L. Butler , Jacqui Sankbeil
IPC分类号： C12K1/08 , C12Q1/68 , C12M1/34 , G01N33/566 , C07H21/02
CPC分类号： C12N15/1006 , C12N1/02 , C12N15/1013 , C12Q1/6806 , Y10T436/143333
摘要： Methods are disclosed for using paramagnetic particles to concentrate or harvest cells. Methods are also disclosed for clearing a solution of disrupted biological material, such as a lysate of cells or a homogenate of mammalian tissue. Methods are also disclosed for using paramagnetic particles to isolate target nucleic acids, such as RNA or DNA, from a solution cleared of disrupted biological material using the same type or a different type of paramagnetic particle. Kits are also disclosed for use with the various methods of the present invention. Nucleic acids isolated according to the present methods and using the present kits are suitable for immediate use in downstream processing, without further purification.

4. 发明授权

US3960664A Bacterial product useful for making sausage 失效
标题翻译：用于制作香肠的细菌产品
公开(公告)号：US3960664A
公开(公告)日：1976-06-01
申请号：US594797
申请日：1975-07-10
申请人： Ronald H. Olsen , Harold Rothchild
发明人： Ronald H. Olsen , Harold Rothchild
IPC分类号： A23L13/40 , C12K1/08
CPC分类号： A23L13/45 , Y10S435/822
摘要： A frozen stabilized bacterial product consisting of Pediococcus cerevisiae mixed with a stabilizing agent, such as glycerol, and a nutrient medium. A process of making a fermented meat product in which the thawed concentrate is mixed with a meat formulation in an amount of at least .1 percent by weight, then the mixture is heated to between 110.degree. and 125.degree.F. for from 8 to 15 hours and then the bacterial action is terminated.
摘要翻译：由稳定剂（例如甘油）和营养培养基混合的由啤酒酵母组成的冷冻稳定的细菌产品。一种制备发酵肉制品的方法，其中将解冻的浓缩物与肉制剂混合至少为0.1重量％，然后将混合物加热至110至125℃，从8至15 小时，然后终止细菌作用。

5. 发明授权

US3541203A Protected virus composition for insect control 失效
标题翻译：保护性病毒组合物
公开(公告)号：US3541203A
公开(公告)日：1970-11-17
申请号：US3541203D
申请日：1969-05-08
申请人： NCR CO
发明人： FOGLE MARK V , PEYTON DAVID G
IPC分类号： A01N25/00 , A01N25/22 , A01N63/00 , C12N7/00 , C12K1/08
CPC分类号： A01N63/00 , A01N2300/00

6. 发明授权

US4325937A Microbial insecticide 失效
公开(公告)号：US4325937A
公开(公告)日：1982-04-20
申请号：US240846
申请日：1981-03-05
申请人： Kemet D. Spence , Robert E. Andrews
发明人： Kemet D. Spence , Robert E. Andrews
IPC分类号： A01N25/28 , A01N63/00 , A61K39/02 , A01N15/00 , A61K39/12 , C12K1/08
CPC分类号： A01N25/28 , A01N63/00 , Y10S514/972
摘要： An improved microbial insecticide composition, and methods for the production and utilization thereof, are disclosed. The disclosed composition comprises a microbial insect pathogen of viral, bacterial, or fungal origin which is susceptible to sunlight-induced inactivation embedded in a coacervate microbead which is comprised of a nucleic acid, typically RNA, and a proteinaceous material, whereby the microbead structure itself effectively shields the agent from sunlight-induced inactivation. The microbead is typically stabilized by chemical crosslinking.

7. 发明授权

US4210672A Preparation of yogurt 失效
标题翻译：准备酸奶
公开(公告)号：US4210672A
公开(公告)日：1980-07-01
申请号：US866396
申请日：1977-12-30
申请人： Kosei Hata
发明人： Kosei Hata
IPC分类号： A23C9/123 , A23C9/12 , C12K1/08 , C12K3/00
CPC分类号： A23C9/1234 , A23C9/123 , Y10S435/832
摘要： Yogurt is prepared with a mixture of milk powder and novel Lactobacillus thermophilus (also known as Bacillus coagulans) spores that have specific characteristics of requiring nourishment at the time of spore formation, being capable of high speed acid formation, taking a short time for transition into germ cells after budding and being highly thermobiotic so as to kill or inhibit during yogurt fermentation the growth of undesirable saprophytes including spore forming saprophytes present in yogurt which serve to putrify or spoil the yogurt. To prepare yogurt, boiling water is added to the mixture to kill or inhibit the growth of saprophytes and the mixture is permitted to ferment for a relatively short period of time.
摘要翻译：酸奶由粉末和新型嗜热乳杆菌（也称为凝结芽孢杆菌）混合物制成，孢子在孢子形成时具有需要营养的特殊特征，能够高速酸形成，需要很短的时间才能过渡到芽殖后的生殖细胞并且是高度生殖的，以便在酸奶发酵过程中杀死或抑制不良营养物质的生长，包括存在于酸奶中的孢子形成腐生菌，其用于使酸奶腐败或破坏。为了制备酸奶，向混合物中加入沸水以杀死或抑制腐生菌的生长，并允许混合物发酵较短的时间。

8. 发明授权

US3671400A Bacterial controls and preparation thereof 失效
标题翻译：细菌控制及其制备
公开(公告)号：US3671400A
公开(公告)日：1972-06-20
申请号：US3671400D
申请日：1969-12-05
申请人： HOFFMANN LA ROCHE
发明人： CEKORIC THOMAS JR , EVANS GEORGE
IPC分类号： C12M1/26 , C12K1/08
CPC分类号： C12M45/22 , C12Q1/04 , G01N2496/25 , Y10S435/805 , Y10S435/81 , Y10S435/849 , Y10S435/873 , Y10S435/875 , Y10S435/883 , Y10S435/884 , Y10S435/885
摘要： Bacteria are preserved by centrifuging a broth culture, mixing the bacterial sediment with gelatin, diethylaminoethyl dextran and monosodium glutamate, and drying at ambient temperature on a non-adhering support surface. The product is useful as a control for test procedures and reagents.
摘要翻译：通过离心培养基培养物，将细菌沉淀物与明胶，二乙基氨基乙基葡聚糖和谷氨酸单钠混合并在环境温度下在不粘附的支撑表面上干燥来保存细菌。该产品可用作测试程序和试剂的对照。

9. 发明授权

US4328203A Microbial insecticide 失效
标题翻译：微生物杀虫剂
公开(公告)号：US4328203A
公开(公告)日：1982-05-04
申请号：US187455
申请日：1980-09-15
申请人： Kemet D. Spence , Robert E. Andrews
发明人： Kemet D. Spence , Robert E. Andrews
IPC分类号： A01N25/28 , A01N63/00 , A61K39/02 , A01N15/00 , A61K39/12 , C12K1/08
CPC分类号： A01N25/28 , A01N63/00 , Y10S514/972
摘要： An improved microbial insecticide composition, and methods for the production and utilization thereof, are disclosed. The disclosed composition comprises a microbial insect pathogen of viral, bacterial, or fungal origin which is susceptible to sunlight-induced inactivation embedded in a coacervate microbead which is comprised of a nucleic acid, typically RNA, and a proteinaceous material, whereby the microbead structure itself effectively shields the agent from sunlight-induced inactivation. The microbead is typically stabilized by chemical crosslinking.
摘要翻译：公开了改进的微生物杀虫剂组合物及其生产和利用方法。所公开的组合物包含病毒，细菌或真菌来源的微生物昆虫病原体，其易于包埋在由核酸（通常为RNA）和蛋白质物质组成的凝聚微球中的阳光诱导的灭活，由此微珠结构本身有效地屏蔽了阳光引起的灭活剂。微珠通常通过化学交联来稳定。

10. 发明授权

US4212943A Production of bacterial cell aggregate 失效
标题翻译：生产细菌细胞聚集体
公开(公告)号：US4212943A
公开(公告)日：1980-07-15
申请号：US890500
申请日：1978-03-27
申请人： Gerald B. Borglum
发明人： Gerald B. Borglum
IPC分类号： C12N9/92 , C12N11/02 , C12N11/08 , C12P19/24 , C12K1/00 , C12K1/08
CPC分类号： C12N11/08 , C12P19/24
摘要： A bacterial cell aggregate having increased particle hardness is produced by contacting a mass of bacterial cells with a cross-linking reaction product of (1) glutaraldehyde, cyanuric halide or combinations thereof and (2) a specific cationic polymer obtained by polymerization of an epihalohydrin and an alkylene polyamine, recovering the resulting aggregate and drying the aggregate.
摘要翻译：通过使大量细菌细胞与（1）戊二醛，氰尿酰卤或其组合的交联反应产物和（2）通过表卤代醇的聚合获得的特定的阳离子聚合物和亚烷基多胺，回收所得聚集体并干燥聚集体。

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