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1. 发明授权

US4115197A Procedure for obtaining penicillium species mutants with improved ability to synthesize mycophenolic acid 失效
标题翻译：获得具有改善合成霉酚酸能力的青霉菌种突变体的方法
公开(公告)号：US4115197A
公开(公告)日：1978-09-19
申请号：US828392
申请日：1977-08-29
申请人： Stephen W. Queener , Claude H. Nash, III
发明人： Stephen W. Queener , Claude H. Nash, III
IPC分类号： C12N15/00 , C12N15/01 , C12P17/04 , C12D9/06 , C12D9/10 , C12K1/02
CPC分类号： C12P17/04 , Y10S435/933
摘要： A procedure for efficiently obtaining higher mycophenolic-acid-producing mutants from mycophenolic-acid-producing strains of the genus Penicillium by selecting mutants which are resistant to polyene antibiotics.
摘要翻译：通过选择对多烯抗生素有抗性的突变体，有效地从青霉属霉菌酸生产菌株中获得更高霉菌酸产生突变体的方法。

2. 发明授权

US4094097A Method for preparing a pesticidally resistant rhizobium and agronomic composition thereof 失效
标题翻译：农药抗性根瘤菌的制备方法及其农艺组合物
公开(公告)号：US4094097A
公开(公告)日：1978-06-13
申请号：US722318
申请日：1976-09-10
申请人： Martin Alexander , Oluwasuyi Odeyemi
发明人： Martin Alexander , Oluwasuyi Odeyemi
IPC分类号： C12N1/36 , C12K1/02 , A01C1/06 , A01C21/00 , C12K3/00
CPC分类号： C12R1/41 , C12N1/36 , Y10S435/878
摘要： This invention relates to Rhizobium strains having good infecting and nitrogen-fixing characteristics and which are resistant to fungicides. These strains are produced by cultivating a Rhizobium strain sensitive to a particular fungicide in the presence of an amount of the fungicide, and for a time less than that sufficient to kill the entire Rhizobium population, but sufficient to kill a majority of the population. The remaining Rhizobium population is isolated and recultured in the presence of an increased amount of the fungicide, again an amount and a time less than sufficient to kill the entire Rhizobium population, but sufficient to kill a majority of the population. This procedure is repeated for sufficient passages, with increasing amounts of said fungicide, to provide a Rhizobium strain sufficiently resistant to said fungicide so that the Rhizobium strain multiplies and enters into a nitrogen-fixing symbiosis in the presence of agriculturally effective amounts of the fungicide.
摘要翻译：本发明涉及具有良好感染和固氮特性并且对杀菌剂具有抗性的根瘤菌属菌株。这些菌株通过在一定量的杀真菌剂的存在下培养对特定杀真菌剂敏感的根瘤菌菌株产生，并且时间小于足以杀死整个根瘤菌群体，但足以杀死大多数群体的时间。剩余的根瘤菌群体在存在增加量的杀真菌剂的情况下被分离和再培养，再次是少于足以杀死整个根瘤菌群体的量和时间，但足以杀死大多数群体。重复该步骤以增加所述杀真菌剂的量，以提供对所述杀真菌剂具有足够的抗性的根瘤菌菌株，使得根瘤菌菌株在农业有效量的杀真菌剂的存在下繁殖并进入固氮共生体。

3. 发明授权

US3923601A Process for the manufacture of cephalosphorin C 失效
标题翻译：头孢菌素C的制备方法
公开(公告)号：US3923601A
公开(公告)日：1975-12-02
申请号：US37953173
申请日：1973-07-16
申请人： CIBA GEIGY CORP
发明人： TREICHLER HANS-JOERG , NUESCH JAKOB
IPC分类号： C12P35/06 , C12K1/02
CPC分类号： C12P35/06 , Y10S435/925
摘要： Process for the manufacture of Cephalosporin C by cultivating mutants of Emericellopsis-Cephalosporium in which the metabolism and/or biosynthesis of organic sulfur compounds is disturbed.
摘要翻译：通过培养有机硫化合物的代谢和/或生物合成受到干扰的脑胶质 - 头孢菌的突变体来制造头孢菌素C的方法。

4. 发明授权

US4172899A Preparation of creamed cottage cheese with a Streptococcus diacetilactis mutant 失效
标题翻译：制备乳酸奶酪与{i链球菌双乙酰胆碱{b突变体
公开(公告)号：US4172899A
公开(公告)日：1979-10-30
申请号：US712692
申请日：1976-08-09
申请人： Ebenezer R. Vedamuthu
发明人： Ebenezer R. Vedamuthu
IPC分类号： A23C19/06 , A23C19/076 , A23C19/02 , A23C19/14 , C12K1/02 , C12K3/00
CPC分类号： C12R1/46 , A23C19/062 , A23C19/076 , Y10S435/885
摘要： A novel mutant strain Streptococcus diacetilactis NRRL-B-8177 which is particularly adapted for the preparation of creamed Cottage cheese without fermentation (or prior incubation) of the creaming mixture or dressing is described. This strain produces about one-half of the acid produced by S. diacetilactis 18-16 which is regarded as the best bacterium that is commercially available for use in Cottage cheese. Improved flavor and prophylaxis against spoilage bacteria of the dressed Cottage cheese is preferably provided by blending a concentrate of the Streptococcus diacetilactis NRRL-B-8177 cells with a creaming mixture at less than about 50.degree. F. (10.degree. C.) and then mixing the cold cream mixture with dry Cottage cheese curd cooled to less than about 50.degree. F. and maintaining the temperature at less than about 50.degree. F.
摘要翻译：描述了一种新颖的突变菌株二链球菌链球菌NRRL-B-8177，其特别适于制备奶油混合物或敷料发酵（或先前孵育）的奶油奶酪。该菌株产生约二分之一的由二乙酰乳杆菌18-16产生的酸，其被认为是商业上可用于酸奶酪的最好的细菌。优选通过在低于约50°F（10℃）下将双链半乳糖链球菌NRRL-B-8177细胞的浓缩物与奶油混合物混合，然后混合，从而提供改良的经过修整的奶酪的腐败细菌的风味和预防措施将含有干燥的奶酪干酪的冷奶油混合物冷却至小于约50°F，并将温度保持在小于约50°F。

5. 发明授权

US3981773A Process for the preparation of galactose and beverages based on galactose from solutions containing lactose 失效
标题翻译：基于含乳糖溶液的半乳糖制备半乳糖和饮料的方法
公开(公告)号：US3981773A
公开(公告)日：1976-09-21
申请号：US489736
申请日：1974-07-18
申请人： Pierre Galzy , Guy Jean Moulin
发明人： Pierre Galzy , Guy Jean Moulin
IPC分类号： A23C9/12 , C12N1/16 , C12N1/20 , C12P19/14 , C13K13/00 , C12D13/02 , C12K1/02
CPC分类号： C12N1/20 , A23C9/1203 , C12N1/16 , C12P19/14 , C13K13/00 , Y10S435/911 , Y10S435/921 , Y10S435/944
摘要： A process for the preparation of galactose which comprises (1) the cultivation of a mutant, non-pathogenic protoprophic yeast or bacteria possessing .beta.-galactosidase activity and a gal.sup.- character on a media containing lactose, e.g., lactoserum and (2) recovery of the galtose. The mutants are produced using two different methods. The galactose solutions produced are suitable for use in beverages.
摘要翻译：一种制备半乳糖的方法，其包括（1）在含有乳糖的培养基（例如乳糖）上培养具有β-半乳糖苷酶活性和gal - 性状的突变型非致病性原生质体酵母或细菌，和（2）恢复的galtose。突变体使用两种不同的方法产生。所生产的半乳糖溶液适用于饮料。

6. 发明授权

US3954536A Process for mutating yeast 失效
标题翻译：突变酵母的方法
公开(公告)号：US3954536A
公开(公告)日：1976-05-04
申请号：US559633
申请日：1975-03-19
申请人： Guido Pacchetti , Epifanio Bacchi
发明人： Guido Pacchetti , Epifanio Bacchi
IPC分类号： C12N15/00 , C12N15/01 , C12R1/645 , C12K1/02
CPC分类号： C12N15/01
摘要： Yeasts are mutated by treatment with 5-fluorouracil after growth in a medium containing glucose followed by growth in a medium containing glycerin.
摘要翻译：在含有葡萄糖的培养基中生长后，在含有甘油的培养基中生长，通过用5-氟尿嘧啶处理使酵母突变。

7. 发明授权

US4159226A Method of obtaining streptomyces protoplasts capable of efficient cell regeneration 失效
标题翻译：获得能够高效细胞再生的链霉菌原生质体的方法
公开(公告)号：US4159226A
公开(公告)日：1979-06-26
申请号：US812084
申请日：1977-07-01
申请人： Richard H. Baltz
发明人： Richard H. Baltz
IPC分类号： C12N15/02 , C12N1/20 , C12N15/03 , C12K1/02
CPC分类号： C12N15/03 , Y10S435/886
摘要： A method of obtaining Streptomyces protoplasts which are able to regenerate viable cells with high efficiency is disclosed. The method involves growing Streptomyces cells to a particular physiological state, the transition phase between the exponential and stationary growth phases, and then forming protoplasts. The physiological state of the cells at the time of protoplast formation is crucial for efficient cell regeneration. Protoplasts obtained by this method enhance the use of protoplast-fusion techniques to effect genetic exchange within the genus Streptomyces, thereby facilitating the construction of hybrid or recombinant Streptomyces strains with useful properties.
摘要翻译：公开了能够以高效率再生活细胞的获得链霉菌原生质体的方法。该方法包括将链霉菌细胞生长至特定生理状态，指数和稳定生长期之间的过渡期，然后形成原生质体。原生质体形成时细胞的生理状态对于有效的细胞再生是至关重要的。通过该方法获得的原生质体增强了原生质体融合技术在链霉菌属中进行遗传交换的用途，从而有助于构建具有有用特性的杂交或重组链霉菌属菌株。

8. 发明授权

US3971701A Method for producing L-glutamic acid by fermentation 失效
标题翻译：通过发酵生产L-谷氨酸的方法
公开(公告)号：US3971701A
公开(公告)日：1976-07-27
申请号：US512480
申请日：1974-10-07
申请人： Koichi Takinami , Takashi Tanaka , Michiaki Chiba , Yoshio Hirose
发明人： Koichi Takinami , Takashi Tanaka , Michiaki Chiba , Yoshio Hirose
IPC分类号： C12P13/18 , C12P13/14 , C12D13/06 , C12B1/20 , C12K1/02
CPC分类号： C12P13/14 , Y10S435/84
摘要： Mutants of glutamic acid producing strains of Brevibacterium which are more sensitive to N-palmitoylglutamic acid than the parent strains require less surfactant to suppress excessive biotin activity in a fermentation medium for permitting glutamic acid production and correspondingly less antifoaming agent without loss of glutamic acid yield.
摘要翻译：与亲本菌株相比，对N-棕榈酰谷氨酸更敏感的谷氨酸生产菌株的突变体需要较少的表面活性剂以抑制发酵培养基中过量的生物素活性，以允许谷氨酸生产，相应地减少消泡剂，而不损失谷氨酸产量。

9. 发明授权

US3957587A Production of xylose (dextrose) isomerase enzyme preparations 失效
标题翻译：生产木糖（葡萄糖）异构酶制剂
公开(公告)号：US3957587A
公开(公告)日：1976-05-18
申请号：US589115
申请日：1975-06-23
申请人： Frederick C. Armbruster , Robert E. Heady , Robert P. Cory
发明人： Frederick C. Armbruster , Robert E. Heady , Robert P. Cory
IPC分类号： C12N9/92 , C12D13/00 , C12D13/10 , C12K1/02
CPC分类号： C12N9/92 , Y10S435/886
摘要： Process for the production of xylose (dextrose) isomerase by means of a mutant strain of Streptomyces that proliferates in a culture medium that may be free of xylose. The xylose (dextrose) isomerase enzyme is therefore constitutive rather than induced.
摘要翻译：通过在可能不含木糖的培养基中增殖的链霉菌突变菌株生产木糖（葡萄糖）异构酶的方法。因此，木糖（葡萄糖）异构酶是组成型的而不是诱导的。

10. 发明授权

US3939042A Process for the production of L-glutamic acid 失效
标题翻译：生产L-谷氨酸的方法
公开(公告)号：US3939042A
公开(公告)日：1976-02-17
申请号：US495272
申请日：1974-08-06
申请人： Kiyoshi Nakayama , Mamoru Kobata , Yoshitake Tanaka , Tadaaki Nomura , Ryoichi Katsumata
发明人： Kiyoshi Nakayama , Mamoru Kobata , Yoshitake Tanaka , Tadaaki Nomura , Ryoichi Katsumata
IPC分类号： C12P13/14 , C12D13/06 , C12K1/02
CPC分类号： C12P13/14 , Y10S435/822 , Y10S435/874
摘要： High yields of L-glutamic acid are produced by culturing mutant strains of microorganisms of the genera Pseudomonas and Protaminobacter in a culture medium containing methanol as the principal source of assimilable carbon. L-glutamic acid is accumulated in the culture liquor and is isolated therefrom.
摘要翻译：通过在含有甲醇作为可同化碳的主要来源的培养基中培养假单胞菌属和Protaminobacter属的微生物的突变菌株来产生高产量的L-谷氨酸。 L-谷氨酸在培养液中积聚并从其中分离出来。

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