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1. 发明授权

US5721120A Method of disrupting cultured cells using an impinging jet device 失效
标题翻译：使用冲击式喷射装置破坏培养细胞的方法
公开(公告)号：US5721120A
公开(公告)日：1998-02-24
申请号：US724802
申请日：1996-10-02
申请人： Douglas B. Seifert , Frank S. Leu
发明人： Douglas B. Seifert , Frank S. Leu
IPC分类号： C12M1/33 , C12M3/08 , C12N1/06 , C12N7/02 , C12D21/06 , C12N5/00 , C12N7/00 , C12D21/02
CPC分类号： C12N7/00 , C12M47/06 , C12N1/06 , C12N2710/16751
摘要： A novel method of disrupting cells which do not have a cell wall comprises passing suspended cells through a low pressure impinging jet device. This method disrupts the cells, but does not harm the cell products which are liberated.
摘要翻译：破坏不具有细胞壁的细胞的新方法包括使悬浮细胞通过低压冲击射流装置。这种方法破坏细胞，但不会伤害被释放的细胞产物。

2. 发明授权

US5710018A Mammalian influx peptide transporter 失效
标题翻译：哺乳动物流入肽转运蛋白
公开(公告)号：US5710018A
公开(公告)日：1998-01-20
申请号：US463345
申请日：1995-06-05
申请人： Anne H. Dantzig , Jo Ann Hoskins , Paul L. Skatrud
发明人： Anne H. Dantzig , Jo Ann Hoskins , Paul L. Skatrud
IPC分类号： C12N5/10 , C07K14/47 , C12N15/09 , C12N15/12 , C12P21/02 , C12Q1/02 , C12R1/91 , C12D21/06
CPC分类号： C07K14/47
摘要： The present invention provides isolated DNA compounds and recombinant DNA vectors that encode mammalian influx peptide transporter activity. The invention also provides host cells transformed with these vectors and a method for production of mammalian influx peptide transporter activity by recombinant DNA techniques. The invention also provides a method for identifying compounds that are transported into the cell by the influx peptide transporter.
摘要翻译：本发明提供分离的DNA化合物和编码哺乳动物流入肽转运蛋白活性的重组DNA载体。本发明还提供了用这些载体转化的宿主细胞和通过重组DNA技术产生哺乳动物流入肽转运蛋白活性的方法。本发明还提供了鉴定通过流入肽转运蛋白被转运到细胞中的化合物的方法。

3. 发明授权

US07101694B2 Genes encoding proteins capable of regenerating luciferin, recombinant DNA and process for producing protein capable of regenerating luciferin 失效
标题翻译：编码能够再生荧光素的蛋白质的基因，重组DNA和产生能够再生荧光素的蛋白质的方法
公开(公告)号：US07101694B2
公开(公告)日：2006-09-05
申请号：US10333740
申请日：2001-07-26
申请人： Kozo Hirokawa , Keiko Kurosawa , Naoki Kajiyama
发明人： Kozo Hirokawa , Keiko Kurosawa , Naoki Kajiyama
IPC分类号： C12P9/02 , C12N15/00 , C12N1/20 , C12D21/06 , C07H21/04
CPC分类号： C12N9/0004 , C07K14/43563
摘要： The present invention relates to:an isolated or synthesized gene, which encodes a protein comprising the amino acid sequence represented by SEQ ID NO: 2,an isolated or synthesized gene, which encodes a protein comprising an amino acid sequence comprising a deletion, substitution or addition of one or more amino acids with respect to the amino acid sequence represented by SEQ ID NO: 2 and being capable of regenerating luciferin,an isolated or synthesized gene, which hybridizes with the complementary strand sequence of a DNA comprising the nucleotide sequence represented by SEQ ID NO: 1 under stringent conditions and encodes a protein capable of regenerating luciferin,a recombinant DNA, which is characterized in that the above-described isolated or synthesized gene is inserted into a vector DNA,a transformant or transductant comprising the above-described recombinant DNA, anda process for producing a protein capable of regenerating luciferin, which is characterized in that the method comprises culturing the above-described transformant or transductant in a medium and collecting therefrom the protein capable of regenerating luciferin.
摘要翻译：本发明涉及：分离或合成的基因，其编码包含SEQ ID NO：2所示的氨基酸序列的蛋白质，分离或合成的基因，其编码包含缺失，取代或取代的氨基酸序列的蛋白质相对于SEQ ID NO：2所示的氨基酸序列添加一个或多个氨基酸，并且能够再生荧光素，一种分离或合成的基因，其与包含以下的核苷酸序列的DNA的互补链序列杂交： SEQ ID NO：1，并编码能够再生荧光素的蛋白质，重组DNA，其特征在于将上述分离或合成的基因插入载体DNA，转化体或包含上述的转导体的转导体重组DNA，以及能够再生荧光素的蛋白质的制造方法，其特征在于， od包括在培养基中培养上述转化体或转导体，并从中收集能够再生萤光素的蛋白质。

4. 发明授权

US5312750A GL-7ACA acylase 失效
标题翻译： GL-7ACA酰基转移酶
公开(公告)号：US5312750A
公开(公告)日：1994-05-17
申请号：US80240
申请日：1993-06-22
申请人： Ichiro Aramori , Masao Fukagawa , Hiroki Ono , Yosuke Ishitani , Mana Tsumura , Morita Iwami , Hitoshi Kojo
发明人： Ichiro Aramori , Masao Fukagawa , Hiroki Ono , Yosuke Ishitani , Mana Tsumura , Morita Iwami , Hitoshi Kojo
IPC分类号： C12N1/21 , C12N9/80 , C12N15/09 , C12N15/55 , C12P35/02 , C12R1/07 , C12R1/19 , C12D21/06 , C12N15/00
CPC分类号： C12N9/80 , C12P35/02
摘要： A GL-7ACA acylase having the following characteristics:(a) has ability to catalyze the enzymatic conversion of glutaryl 7-ACA, adipyl 7-ACA, and succinyl 7-ACA, into 7-aminocephalosporanic acid,(b) has a molecular weight of 70,000 dalton (SDS-PAGE) and(c) has N-terminal amino acid sequence (sea in No: 1) thereof: Gln-Ser-Glu-Gln-Glu-Lys-Ala-Glu-Glu-.A process for producing GL-7ACA acylase is also provided.
摘要翻译：具有以下特征的GL-7ACA酰基转移酶：（a）具有催化戊二酰7-ACA，己二酰基7-ACA和琥珀酰基7-ACA酶促转化成7-氨基头孢烷酸的能力，（b）具有分子量的70,000道尔顿（SDS-PAGE）和（c）具有N-末端氨基酸序列（海洋号：1）：Gln-Ser-Glu-Gln-Glu-Lys-Ala-Glu-Glu-。还提供了生产GL-7ACA酰基转移酶的方法。

5. 发明授权

US07214523B2 Method for production of S-hydroxynitrile lyase by use of Escherichia coli 失效
标题翻译：使用大肠杆菌生产S-羟基腈裂解酶的方法
公开(公告)号：US07214523B2
公开(公告)日：2007-05-08
申请号：US10738927
申请日：2003-12-16
申请人： Hisashi Semba , Eita Ichige , Masaharu Mukouyama
发明人： Hisashi Semba , Eita Ichige , Masaharu Mukouyama
IPC分类号： C12D21/06 , C12N9/88 , C12N1/20 , C07H21/02 , A61K35/78
CPC分类号： C12N9/88
摘要： A method for efficient production of an S-hydroxynitrile lyase by gene recombination using Escherichia coli is provided. A gene is formed by altering a codon in an S-hydroxyniitrylase gene originating in cassava (Manihot esculenta Crantz) without changing the amino acid sequence thereof till the frequency of codon usage wholly with an amino acid in Escherichia coli reaches a level of not less than 5%. According to the method for the production of an S-hydroxynitrile lyase by the use of the gene, the S-hydroxynitrile lyase can be produced in a large amount with an unusually high yield.
摘要翻译：提供了使用大肠杆菌通过基因重组有效生产S-羟基腈裂解酶的方法。通过改变源自木薯（Manihot esculenta Crantz）的S-羟基亚硝基酯基因中的密码子而不改变其氨基酸序列而形成基因，直到完全用大肠杆菌中的氨基酸进行密码子使用的频率达到不低于 5％。根据通过使用该基因生产S-羟基腈裂解酶的方法，可以以异常高的产率大量生产S-羟基腈裂解酶。

6. 发明授权

US06982159B2 Trichoderma β-glucosidase 有权
标题翻译：木霉属β-葡糖苷酶
公开(公告)号：US06982159B2
公开(公告)日：2006-01-03
申请号：US09957880
申请日：2001-09-21
申请人： Nigel Dunn-Coleman , Frits Goedegebuur , Michael Ward , Jian Yao
发明人： Nigel Dunn-Coleman , Frits Goedegebuur , Michael Ward , Jian Yao
IPC分类号： C12N9/00 , C12N9/24 , C12D21/06 , C07H21/04
CPC分类号： A21D8/042 , C11D3/38645 , C12N9/2445 , C12N15/1137 , C12Y302/01021 , Y02E50/17
摘要： The present invention provides a novel β-glucosidase nucleic acid sequence, designated bgl3, and the corresponding BGL3 amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding BGL3, recombinant BGL3 proteins and methods for producing the same.
摘要翻译：本发明提供了命名为bgl3的新型β-葡糖苷酶核酸序列和相应的BGL3氨基酸序列。本发明还提供了包含编码BGL3的核酸序列，重组BGL3蛋白的表达载体和宿主细胞及其制备方法。

7. 发明授权

US5863763A Insect neuropeptides 失效
标题翻译：昆虫神经肽
公开(公告)号：US5863763A
公开(公告)日：1999-01-26
申请号：US714053
申请日：1996-09-11
申请人： Hanne Duve , Alan Thorpe , Anders Holten Johnsen
发明人： Hanne Duve , Alan Thorpe , Anders Holten Johnsen
IPC分类号： C12N15/09 , A01N37/18 , A01N63/00 , A01N63/02 , C07K7/06 , C07K7/08 , C12D21/06 , A61K38/00 , A61K38/04 , C07K5/00
CPC分类号： C07K7/06 , A01N63/02 , C07K7/08
摘要： The present invention relates to novel peptides isolated from the blowfly Calliphora vomitoria. The invention also relates to nucleic acid molecules encoding these peptides, vectors and host cells comprising these nucleic acid molecules, and chemical and biological methods for producing these peptides. The present peptides have novel sequences comprising hydroxyproline residues and C-terminal amide groups which give the peptides improved stability in the gut of insects. The peptides of the invention are related to the allatostatin class of insect neuropeptides, have inhibitory effects on gut motility in the blowfly C. vomitoria, and have allatostatic effects in cockroaches of the species Diploptera punctata and Periplaneta americana. The invention also provides compositions comprising the present peptides which may be used as insecticides, and provides methods of killing or controlling insects comprising administering the nucleic acid molecules, peptides or compositions of the invention to insects.
摘要翻译： PCT No.PCT / GB95 / 00505 Sec。 371日期：1996年9月11日 102（e）日期1996年9月11日PCT Filed Mar. 9，1995 PCT Pub。公开号WO95 / 24423 1995年9月14日发明内容本发明涉及从泡虱Calliphora vonitoria分离的新型肽。本发明还涉及编码这些肽的核酸分子，包含这些核酸分子的载体和宿主细胞，以及用于产生这些肽的化学和生物学方法。本发明的肽具有包含羟脯氨酸残基和C-末端酰胺基团的新型序列，其使得肽在昆虫肠中的稳定性提高。本发明的肽与昆虫神经肽的维甲酸酯抑制剂有关，对飞虱嗜酒的肠道蠕动具有抑制作用，并且对Dip虫和美洲大蠊的蟑螂具有抑制作用。本发明还提供了包含可用作杀虫剂的本发明肽的组合物，并提供了杀死或控制昆虫的方法，包括向昆虫施用本发明的核酸分子，肽或组合物。

8. 发明授权

US5747329A Glutamylcysteine synthetase light subunit 失效
标题翻译：谷氨酰半胱氨酸合成酶轻亚基
公开(公告)号：US5747329A
公开(公告)日：1998-05-05
申请号：US350325
申请日：1994-12-05
申请人： Alton Meister , Chin-Shiou Huang , Mary E. Anderson
发明人： Alton Meister , Chin-Shiou Huang , Mary E. Anderson
IPC分类号： A61K38/00 , C12N9/00 , C12N1/20 , C07H21/04 , C12D21/06
CPC分类号： C12N9/93 , A61K38/00
摘要： The nucleotide cDNA sequence for the light subunit of gamma glutamylcysteine synthetase, and the amino acid sequence for this subunit, are disclosed
摘要翻译：公开了γ-谷氨酰半胱氨酸合成酶的轻亚基的核苷酸cDNA序列和该亚基的氨基酸序列

9. 发明授权

US5658757A Cloned leptospira outer membrane protein 失效
标题翻译：克隆的钩端螺旋体外膜蛋白
公开(公告)号：US5658757A
公开(公告)日：1997-08-19
申请号：US362739
申请日：1994-12-20
申请人： David A. Haake , David R. Blanco , Cheryl I. Champion , Michael A. Lovett , James N. Miller
发明人： David A. Haake , David R. Blanco , Cheryl I. Champion , Michael A. Lovett , James N. Miller
IPC分类号： A61K39/00 , C07K14/20 , C12N15/00 , C12N5/00 , C12D21/06 , C07H19/00
CPC分类号： C07K14/20 , A61K39/00 , Y10S435/81 , Y10S530/825
摘要： An antigenic preparation is provided which contains a 31 Kd outer membrane protein from Leptospira which can be used immunologically as a vaccine for leptospirosis caused by this organism.
摘要翻译：提供了一种抗原制剂，其含有来自钩端螺旋体的31Kd外膜蛋白，其可以免疫学地用作由该生物体引起的钩端螺旋体病毒的疫苗。

10. 发明授权

US5614381A Method for the inducible production of proteins in genetically modified eukaryotic host-cells multiplied in vivo 失效
标题翻译：在遗传修饰的真核宿主细胞中可诱导产生蛋白质的方法在体内倍增
公开(公告)号：US5614381A
公开(公告)日：1997-03-25
申请号：US421277
申请日：1995-04-13
申请人： Peter Bromley , Michel Dreano , Michel Fischbach , Xavier Fouillet , Prudent Padieu , Richard Voellmy
发明人： Peter Bromley , Michel Dreano , Michel Fischbach , Xavier Fouillet , Prudent Padieu , Richard Voellmy
IPC分类号： C12N15/09 , A01K67/027 , C07K14/61 , C12N5/00 , C12N5/10 , C12N15/00 , C12N15/85 , C12P21/00 , C12P21/02 , C12R1/91 , A01N63/00 , C12D21/06
CPC分类号： C07K14/61 , A01K67/0271 , C12N15/85 , A01K2217/05 , C12N2830/002 , C12N2830/85
摘要： In the production of proteins of biological interest by means of a stress inducible gene expression unit/eukaryotic host cell system, the transformed cell lines are multiplicated by tumour growing in immunodefficient warm-blooded animals, after which the multiplicated cells are cultured in vitro and subjected to stress, whereby expression occurs in high yield. In vivo multiplication rates of 10.sup.5 -10.sup.6 the innoculated quantity/2 weeks are reported without any loss of the latent inducible expression capacity.
摘要翻译：在通过应激诱导型基因表达单元/真核宿主细胞系统生产生物感兴趣的蛋白质中，通过在免疫缺陷的温血动物中生长肿瘤将转化的细胞系倍增，之后将多重细胞体外培养并进行压力，从而表达以高产量发生。报道了105-106的接种量/ 2周的体内增殖率，没有潜在的诱导表达能力的任何损失。

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