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1. 发明授权

US07312378B2 Nucleic acids encoding heat stable mutants of plant ADP-glucose pyrophosphorylase 失效
标题翻译：编码植物ADP-葡萄糖焦磷酸化酶热稳定突变体的核酸
公开(公告)号：US07312378B2
公开(公告)日：2007-12-25
申请号：US10970230
申请日：2004-10-20
申请人： L. Curtis Hannah , Thomas W. Greene
发明人： L. Curtis Hannah , Thomas W. Greene
IPC分类号： A01N5/00 , A01N5/10 , C12N15/82 , C12N15/52
CPC分类号： C12N9/1241 , C12N9/1051 , C12N15/8245 , C12N15/8271 , C12Y204/01021 , C12Y207/07027
摘要： The subject invention pertains to novel mutant polynucleotide molecules that encode enzymes that have increased heat stability. These polynucleotides, when expressed in plants, result in increased yield in plants grown under conditions of heat stress. The polynucleotide molecules of the subject invention encode maize endosperm ADP glucose pyrophosphorylase (AGP) and soluble starch synthase (SSS) enzyme activities. Plants and plant tissue bred to contain, or transformed with, the mutant polynucleotides, and expressing the polypeptides encoded by the polynucleotides, are also contemplated by the present invention. The subject invention also concerns methods for isolating polynucleotides and polypeptides contemplated within the scope of the invention. Methods for increasing yield in plants grown under conditions of heat stress are also provided.
摘要翻译：本发明涉及编码具有增加的热稳定性的酶的新型突变体多核苷酸分子。当在植物中表达时，这些多核苷酸导致在热胁迫条件下生长的植物中的产量增加。本发明的多核苷酸分子编码玉米胚乳ADP葡萄糖焦磷酸化酶（AGP）和可溶性淀粉合成酶（SSS）酶活性。繁殖含有突变体多核苷酸或转化了突变多核苷酸并表达多核苷酸编码的多肽的植物和植物组织也被本发明考虑。本发明还涉及分离本发明范围内考虑的多核苷酸和多肽的方法。还提供了在热胁迫条件下生长的植物中提高产量的方法。

2. 发明授权

US07371924B2 Nucleic acid sequences encoding β-ketoacyl-ACP synthase and uses thereof 有权
标题翻译：编码β-酮酰基-ACP合酶的核酸序列及其用途
公开(公告)号：US07371924B2
公开(公告)日：2008-05-13
申请号：US10735242
申请日：2003-12-12
申请人： Katayoon Dehesh
发明人： Katayoon Dehesh
IPC分类号： C12N15/29 , C12N15/52 , C12N15/82 , A01H5/00 , A01N5/10
CPC分类号： C12N9/1029 , A23D9/00 , C11B1/00 , C12N15/8247 , C12P7/6409 , C12P7/6463
摘要： This invention relates to sequences encoding β-ketoacyl-ACP synthase (KAS) and methods of use thereof. Also provided are methods for decreasing saturated fatty acid levels as a component of total triglycerides found in plant oils. The method generally comprises growing a soybean plant having integrated into its genome a DNA construct comprising, in the 5′ to 3′ direction of transcription, a promoter functional in a soybean plant seed cell, a DNA sequence encoding a KAS protein, and a transcription termination region functional in a plant cell. The present invention also provides a soybean seed with less than about 3.5 weight percent total saturated fatty acids.
摘要翻译：本发明涉及编码β-酮酸酰-ACP合酶（KAS）的序列及其使用方法。还提供了降低饱和脂肪酸水平作为在植物油中发现的总甘油三酯的组分的方法。该方法通常包括生长大豆植物，其整合到其基因组中，DNA构建体包含在转录的5'至3'方向上在大豆植物种子细胞中起作用的启动子，编码KAS蛋白的DNA序列和转录终止区在植物细胞中起作用。本发明还提供了具有小于约3.5重量％的总饱和脂肪酸的大豆种子。

3. 发明授权

US07253343B2 AXMI-003, a delta-endotoxin gene and methods for its use 有权
标题翻译： AXMI-003，一种δ-内毒素基因及其使用方法
公开(公告)号：US07253343B2
公开(公告)日：2007-08-07
申请号：US10926819
申请日：2004-08-26
申请人： Nadine Carozzi , Tracy Hargiss , Michael G. Koziel , Nicholas B. Duck , Brian Carr
发明人： Nadine Carozzi , Tracy Hargiss , Michael G. Koziel , Nicholas B. Duck , Brian Carr
IPC分类号： A01N5/00 , A01N5/10 , C12N15/32 , C12N15/82 , C12P21/04
CPC分类号： C07H21/04 , C07K14/325
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2 or 4, or the nucleotide sequence set forth in SEQ ID NO:1 or 3, as well as variants thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含δ-内毒素多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的δ-内毒素核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了包含编码SEQ ID NO：2或4所示的氨基酸序列的核苷酸序列或SEQ ID NO：1或3所示的核苷酸序列的分离的核酸分子及其变体。

4. 发明授权

US06891086B2 Plastid transformation of Brassica 有权
标题翻译：芸苔的质体转化
公开(公告)号：US06891086B2
公开(公告)日：2005-05-10
申请号：US10219227
申请日：2002-08-15
申请人： Sumita Chaudhuri , Janette V. Oakes
发明人： Sumita Chaudhuri , Janette V. Oakes
IPC分类号： A01H5/00 , C12N5/10 , C12N15/09 , C12N15/29 , C12N15/82 , C12N15/12 , C12N15/31 , A01N5/00 , A01N5/10
CPC分类号： C12N15/8214
摘要： A method is provided for transforming Brassica plants to express DNA sequences of interest from the plant cell plastid. The method allows the transformation of Brassica plant tissue with heterologous DNA constructs. Such DNA constructs comprise, in the 5′ to 3′ direction of transcription, a promoter region functional in a plant plastid and a DNA sequence of interest. The invention further provides for Brassica cells in which the plastids contain heterologous DNA constructs.
摘要翻译：提供了用于转化芸苔属植物以从植物细胞质体表达感兴趣的DNA序列的方法。该方法允许用异源DNA构建体转化芸苔属植物组织。这样的DNA构建体在5'到3'的转录方向上包含在植物质体中有功能的启动子区和感兴趣的DNA序列。本发明还提供了其中质体含有异源DNA构建体的芸苔属细胞。

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