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    • 10. 发明授权
    • Digital holographic microscope
    • 数字全息显微镜
    • US07463366B2
    • 2008-12-09
    • US11284768
    • 2005-11-22
    • Franck DuboisCatherine Yourassowsky
    • Franck DuboisCatherine Yourassowsky
    • G01B9/02
    • G01N21/6458G02B21/16G02B21/365G03H1/0005G03H1/041G03H1/0443G03H1/0465G03H1/0866G03H2001/005G03H2001/0452G03H2001/0456G03H2001/0458G03H2222/24G03H2223/14G03H2223/26
    • A method and device for obtaining a sample with three-dimensional microscopy, in particular a thick biological sample and the fluorescence field emitted by the sample. One embodiment includes obtaining interferometric signals of a specimen, obtaining fluorescence signals emanating from the specimen, recording these signals, and processing these signals so as to reconstruct three-dimensional images of the specimen and of the field of fluorescence emitted by the specimen at a given time. Another embodiment includes a digital holography microscope, a fluorescence excitation source illuminating a specimen, where the microscope and the fluorescence excitation source cooperate to obtain interferometric signals of the specimen and obtain fluorescence signals emanating from the specimen, means for recording the interferometric signals and fluorescence signals, and means for processing the interferometric signals and the fluorescence signals so as to reconstruct three-dimensional images of the specimen and of the field of fluorescence emitted by the specimen at a given time.
    • 一种利用三维显微镜获得样品的方法和装置,特别是厚的生物样品和由样品发射的荧光场。 一个实施例包括获得样本的干涉信号,获得从样本发出的荧光信号,记录这些信号,以及处理这些信号,以便重建样本的三维图像和由给定的样本发射的荧光场 时间。 另一个实施例包括数字全息显微镜,照射样本的荧光激发源,其中显微镜和荧光激发源协作以获得样本的干涉信号并获得从样本发出的荧光信号,用于记录干涉信号和荧光信号的装置 以及用于处理干涉信号和荧光信号的装置,以便重建样本的三维图像和在给定时间由样本发射的荧光场。