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    • 7. 发明申请
    • DATA ACQUISITION METHOD USING A LASER SCANNER
    • 使用激光扫描仪的数据采集方法
    • US20110006219A1
    • 2011-01-13
    • US12666035
    • 2009-02-13
    • Alois SchausbergerAndreas GfrörerWolfgang Fuchs
    • Alois SchausbergerAndreas GfrörerWolfgang Fuchs
    • G01T1/10
    • G02B21/26G01N21/278G01N21/6458G01N2021/6419G01N2201/104G01N2201/11G02B21/0076
    • The invention relates to a data acquisition method using a laser scanner for the pixel-precise imaging of fluorescent samples which are on object carriers and have been treated with fluorescent dyes. Such a laser scanner comprises a sample table; at least one laser and a first optical system for providing at least one laser beam for exciting the fluorescent samples; a scanner head (50) having an optical deflecting element for scanning this sample in at least one direction of movement (75); a first lens; a second optical system for forwarding emission beams, which are triggered by the laser beams on the sample and are deflected by the first lens and the deflecting element, to at least one detector; a position encoder (91) which emits position encoder signals (92) which indicate the instantaneous location of the scanner head (50) in relation to a zero point; an electronic element for filtering the detector signals (93) with a defined time constant; and an A/D converter for digitizing the filtered detector signals (93). The data acquisition method according to the invention is characterized in that the filtered detector signals (93) from the A/D converter and the position encoder signals (92) are acquired independently, in a parallel manner and continuously by a computer unit or a controller (40) and are related to a common time base (94), wherein the A/D conversion is carried out so often that each pixel (95) of an image is always assigned more than one data point of the A/D converter.
    • 本发明涉及一种使用激光扫描仪进行像素精确成像的数据采集方法,其中荧光样品位于物体载体上并用荧光染料处理。 这种激光扫描器包括样品台; 至少一个激光器和第一光学系统,用于提供用于激发荧光样品的至少一个激光束; 扫描头(50)具有用于在至少一个运动方向(75)扫描该样品的光学偏转元件; 第一个镜头; 用于转发发射光束的第二光学系统,其由样品上的激光束触发并被第一透镜和偏转元件偏转到至少一个检测器; 位置编码器(91),其发射指示扫描器头(50)相对于零点的瞬时位置的位置编码器信号(92) 用于以确定的时间常数对检测器信号(93)进行滤波的电子元件; 以及用于数字化滤波的检测器信号(93)的A / D转换器。 根据本发明的数据获取方法的特征在于,来自A / D转换器的滤波检测器信号(93)和位置编码器信号(92)以并行方式独立地获取并且由计算机单元或控制器 (40)并且与公共时基(94)相关,其中所述A / D转换经常被执行,使得图像的每个像素(95)总是被分配多个A / D转换器的一个数据点。