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    • 10. 发明申请
    • SIZE DEPENDENCE OF NANOPARTICLE-PROTEIN INTERACTION AND HIGH SENSITIVITY POTENTIOMETRIC SENSOR EXPLOITING SUCH INTERACTIONS
    • 纳米尺度蛋白质相互作用的大小依赖性和高灵敏度电位传感器开发这种相互作用
    • US20160341686A1
    • 2016-11-24
    • US15156648
    • 2016-05-17
    • Kalle LEVONYanyan Wang
    • Kalle LEVONYanyan Wang
    • G01N27/327C25D15/00C12Q1/00
    • C12Q1/006B82Y30/00C12Q1/003C25D3/48C25D5/18C25D5/54G01N27/3278G01N2333/904
    • Fabrication of a high sensitivity potentiometric biosensor is described. The present inventors have developed and characterized a novel amplification platform using a gold nanoparticle (GNPs) electrodeposition method. The synthesized GNP sizes were found to be dependent of HAuCl4 concentration, media acid, scan cycles and scan rate. A systematic investigation into the adsorption of different sizes of proteins from aqueous electrolyte solution onto the electrodeposited GNPs surface by the potentiometric method was performed. Results suggest that the size of different proteins affect how they bond to different sizes of GNPs. This GNPs-based biosensor can retain the native-like structure of proteins, and successfully detect proteins at a high sensitivity level. The resulting glucose and immune biosensors also exhibit low detection limit and wide linear range. This improvement to potentiometric devices enables them to serve as highly sensitive detectors for biomolecules and provides a model that can be used to predict protein bonding on nanoparticles.
    • 描述了高灵敏度电位生物传感器的制造。 本发明人已经开发并表征了一种使用金纳米颗粒(GNP)电沉积方法的新型扩增平台。 发现合成的GNP大小取决于HAuCl4浓度,培养基酸,扫描周期和扫描速率。 进行了通过电位法对电沉积的GNP表面吸附不同大小蛋白质的电解质溶液的系统研究。 结果表明,不同蛋白质的大小影响它们如何结合不同大小的GNP。 这种基于GNP的生物传感器可以保留蛋白质的天然样结构,并成功地检测高灵敏度水平的蛋白质。 所得到的葡萄糖和免疫生物传感器也具有低的检测限和宽的线性范围。 这种对电位器件的改进使其能够用作生物分子的高灵敏度检测器,并提供可用于预测纳米粒子上的蛋白质结合的模型。