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    • 4. 发明申请
    • COVALENT MODIFICATION OF BIOLOGICAL MACROMOLECULES
    • 生物大分子的共价修饰
    • US20140273153A1
    • 2014-09-18
    • US14210106
    • 2014-03-13
    • Mosaic Biosciences, Inc.
    • Alexei KAZANTSEVPeter D. MARINERMartin STANTON
    • C07K14/75C07K1/107A61K47/48C12N9/96
    • C07K14/75A61K47/557A61K47/60C07K1/1077C12N9/63C12N9/96C12Y304/22006
    • The present disclosure provides a method of covalently modifying a biological macromolecule, the method comprising subjecting a reaction mixture comprising: (a) a biological macromolecule comprising one or more thiol groups; and (b) a molecule comprising one or more olefin or alkyne moieties to a radical reaction under conditions sufficient to produce the covalently modified biological macromolecule. The present disclosure also provides a method of covalently modifying a biological macromolecule, the method comprising subjecting a reaction mixture comprising: (a) a molecule comprising one or more thiol groups; and (b) a biological macromolecule comprising one or more olefin or alkyne moieties to a radical reaction under conditions sufficient to produce the covalently modified biological macromolecule. The present disclosure further provides a covalently modified biological macromolecule prepared by any of the disclosed methods. The covalently modified biological macromolecules may be further crosslinked to form a scaffold.
    • 本公开提供共价修饰生物大分子的方法,所述方法包括使包含以下物质的反应混合物:(a)包含一个或多个硫醇基的生物大分子; 和(b)在足以产生共价修饰的生物大分子的条件下,包含一个或多个烯烃或炔部分至自由基反应的分子。 本公开还提供共价修饰生物大分子的方法,该方法包括使包含以下物质的反应混合物:(a)包含一个或多个硫醇基团的分子; 和(b)在足以产生共价修饰的生物大分子的条件下,在自由基反应中含有一个或多个烯烃或炔部分的生物大分子。 本公开进一步提供了通过任何所公开的方法制备的共价修饰的生物大分子。 共价修饰的生物大分子可进一步交联以形成支架。