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1. 发明申请

US20120034666A1 METHODS OF INCREASING DIHYDROXY ACID DEHYDRATASE ACTIVITY TO IMPROVE PRODUCTION OF FUELS, CHEMICALS, AND AMINO ACIDS 审中-公开
标题翻译：增加二羟酸脱水酶活性的方法，以提高燃料，化学品和氨基酸的生产
公开(公告)号：US20120034666A1
公开(公告)日：2012-02-09
申请号：US13279166
申请日：2011-10-21
申请人： Andrew C. HAWKINS , Aristos Aristidou , Doug Lies , Lynne H. Albert
发明人： Andrew C. HAWKINS , Aristos Aristidou , Doug Lies , Lynne H. Albert
IPC分类号： C12P7/16 , C12N1/19
CPC分类号： C12N9/88 , C07K14/395 , C12N9/13 , C12P7/16 , C12P13/02 , C12P13/06 , C12P13/08 , C12Y208/01007 , Y02E50/10
摘要： The present invention is directed to recombinant microorganisms comprising one or more dihydroxyacid dehydratase (DHAD)-requiring biosynthetic pathways and methods of using said recombinant microorganisms to produce beneficial metabolites derived from said DHAD-requiring biosynthetic pathways. In various aspects of the invention, the recombinant microorganisms may be engineered to overexpress one or more polynucleotides encoding one or more Nfs1 proteins or homologs thereof and/or one or more polynucleotides encoding one or more Isd11 proteins or homologs thereof. In some embodiments, the recombinant microorganisms may comprise a cytosolically localized DHAD enzyme. In additional embodiments, the recombinant microorganisms may comprise a mitochondrially localized DHAD enzyme. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.
摘要翻译：本发明涉及包含一种或多种二羟基酸脱水酶（DHAD） - 生物合成途径的重组微生物，以及使用所述重组微生物产生源自所述DHAD需要生物合成途径的有益代谢物的方法。在本发明的各个方面，重组微生物可被改造以过表达一种或多种编码一种或多种Nfs1蛋白或其同系物的多核苷酸和/或一种或多种编码一种或多种Isd11蛋白或其同系物的多核苷酸。在一些实施方案中，重组微生物可以包含细胞溶质定位的DHAD酶。在另外的实施方案中，重组微生物可以包含线粒体定位的DHAD酶。在本文所述的各种实施方案中，重组微生物可以是酵母进化枝，Crabtree阴性酵母微生物，Crabtree阳性酵母微生物，WGD（全基因组复制）酵母微生物，WGD前（全基因组复制）酵母微生物，和非发酵酵母微生物。

2. 发明授权

US12024736B2 Method for detecting l-serine based on 有权
公开(公告)号：US12024736B2
公开(公告)日：2024-07-02
申请号：US17814443
申请日：2022-07-22
申请人： Wenzhou Medical University
发明人： Guoqiang Tan , Jianghui Li , Feng Liang , Yilin Pang
IPC分类号： C12Q1/48 , G01N33/68
CPC分类号： C12Q1/48 , C12Y208/01007 , G01N33/6812 , G01N2333/91194
摘要： The present disclosure provides a method for detecting L-serine based on Escherichia coli cysteine desulfurase, and belongs to the technical field of amino acid detection. The method includes the steps of: reacting an unknown sample containing L-serine with E. coli cysteine desulfurase in vitro to produce a red substance, and qualitatively or quantitatively determining L-serine content in the unknown sample by observing a color of the red substance or determining content thereof. The method provided by the present disclosure is simple and feasible in technical operation, few in reaction steps, and capable of directly qualitatively detecting by naked eyes and quantitatively detecting the L-serine content.

3. 发明授权

US11859236B2 Method for detecting L-serine based on cysteine desulfurase-containing living 有权
公开(公告)号：US11859236B2
公开(公告)日：2024-01-02
申请号：US17811600
申请日：2022-07-11
申请人： WENZHOU MEDICAL UNIVERSITY
发明人： Guoqiang Tan , Jianghui Li , Feng Liang , Yilin Pang
IPC分类号： C12Q1/48 , C12N9/10 , C12N15/70
CPC分类号： C12Q1/48 , C12N9/13 , C12N15/70 , C12Y208/01007 , C12N2800/101
摘要： The present disclosure provides a method for detecting L-serine based on cysteine desulfurase-containing living Escherichia coli cells, and belongs to the technical field of amino acid detection. The method includes the following steps: incubating an unknown sample with the cysteine desulfurase-containing living E. coli cells to produce a red substance, and qualitatively or semi-quantitatively detecting L-serine content in the unknown sample according to color changes of the red substance of the living E. coli cells, or quantitatively detecting L-serine content in the unknown sample by measuring absorbance of a lysate of the living E. coli cells. The detection method provided by the present disclosure is simple and convenient in process, few in reaction steps and stable in enzymatic activity of living cells.

4. 发明公开

US20230145499A1 METHOD FOR DETECTING L-SERINE BASED ON ESCHERICHIA COLI CYSTEINE DESULFURASE 审中-公开
公开(公告)号：US20230145499A1
公开(公告)日：2023-05-11
申请号：US17814443
申请日：2022-07-22
申请人： Wenzhou Medical University
发明人： Guoqiang TAN , Jianghui Li , Feng Liang , Yilin Pang
IPC分类号： C12Q1/48 , G01N33/68
CPC分类号： C12Q1/48 , C12Y208/01007 , G01N33/6812 , G01N2333/91194
摘要： The present disclosure provides a method for detecting L-serine based on Escherichia coli cysteine desulfurase, and belongs to the technical field of amino acid detection. The method includes the steps of: reacting an unknown sample containing L-serine with E. coli cysteine desulfurase in vitro to produce a red substance, and qualitatively or quantitatively determining L-serine content in the unknown sample by observing a color of the red substance or determining content thereof. The method provided by the present disclosure is simple and feasible in technical operation, few in reaction steps, and capable of directly qualitatively detecting by naked eyes and quantitatively detecting the L-serine content.

5. 发明申请

US20140308711A1 ENZYMATIC PHOSPHOROTHIOATION OF DNA OR RNA 审中-公开
标题翻译： DNA或RNA的酶促磷酸化
公开(公告)号：US20140308711A1
公开(公告)日：2014-10-16
申请号：US14210188
申请日：2014-03-13
申请人： Massachusetts Institute of Technology , Wuhan University , Shanghai Jiaotong University
发明人： Peter C. Dedon , Delin You , Bo Cao , Ramesh Babu Indrakanti , Michael S. DeMott , Lianrong Wang , Zixin Deng , Xiufen Zhou
IPC分类号： C12N15/64 , C12N9/10
CPC分类号： C12N15/64 , C12N9/13 , C12P11/00 , C12P19/34 , C12Y208/01007
摘要： This invention relates to methods, compositions, and kits for enzymatic phosphorothioation of the sugar-phosphate backbone of nucleic acids. The invention allows for phosphorothioation of pre-existing nucleic acids.
摘要翻译：本发明涉及用于核酸的糖 - 磷酸骨架酶的硫代磷酸化的方法，组合物和试剂盒。本发明允许预先存在的核酸的硫代磷酸化。

6. 发明公开

US20240117326A1 EXPRESSION OF NITROGENASE POLYPEPTIDES IN PLANT CELLS 审中-公开
公开(公告)号：US20240117326A1
公开(公告)日：2024-04-11
申请号：US17767838
申请日：2020-10-09
申请人： Commonwealth Scientific and Industrial Research Organisation
发明人： Ema Jayne JOHNSTON , Robert Silas ALLEN , Christina Maria GREGG , Shoko OKADA , Amratha MENON , Andrew Charles WARDEN , Matthew Craig TAYLOR , Craig Christopher WOOD
IPC分类号： C12N9/02 , C07K14/415 , C12N9/10 , C12N9/24 , C12N15/62 , C12N15/82
CPC分类号： C12N9/0095 , C07K14/415 , C12N9/1025 , C12N9/13 , C12N9/2402 , C12N15/62 , C12N15/8243 , C12Y118/06001 , C12Y203/03014 , C12Y208/01007 , C07K2319/07 , C07K2319/50
摘要： The present invention relates to methods and means for producing nitrogenase polypeptides in the mitochondria of plant cells.

7. 发明申请

US20170355971A1 NOVEL THERMOPHILE-DERIVED KERATINASE AND USE THEREOF 审中-公开
公开(公告)号：US20170355971A1
公开(公告)日：2017-12-14
申请号：US15509095
申请日：2015-09-07
申请人： KYUNGPOOK NATIONAL UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION
发明人： Dong Woo Lee , Yong Jik Lee , Hyun Su Jin , Ji Yeon Kim , Gae Won Nam , Sang Jae Lee
IPC分类号： C12N9/10 , C12N9/48 , C12N9/02
CPC分类号： C12N9/13 , C12N9/00 , C12N9/0051 , C12N9/48 , C12N9/485 , C12N15/52 , C12Y108/01009 , C12Y208/01007 , C12Y304/16005
摘要： The present invention relates to a novel thermophile-derived keratinase having keratin decomposition ability. Further, the present invention relates to a polynucleotide encoding the keratinase, a recombinant vector containing the same, host cells transformed by using the recombinant vector, and a method for preparing a keratinase including a step of culturing the host cells. Further, the present invention relates to a composition for decomposing keratin containing the enzyme; and a method for decomposing keratin by using the same. Further, the present invention relates to a keratin decomposed product decomposed by the enzyme.The keratinase according to the present invention rapidly and effectively decomposes hardly-decomposable keratin, and thus it is expected that the keratinase can be used for the effective treatment and the high value-added resource recovery of agricultural and livestock waste, which causes environmental problems (for example, a novel material for enzyme cosmetics), and can be used in an innovative enzymatic bioconversion technique utilizing various decomposition enzyme groups.

8. 发明公开

US20230159980A1 METHOD FOR DETECTING L-SERINE BASED ON CYSTEINE DESULFURASE-CONTAINING LIVING ESCHERICHIA COLI CELL 审中-公开
公开(公告)号：US20230159980A1
公开(公告)日：2023-05-25
申请号：US17811600
申请日：2022-07-11
申请人： WENZHOU MEDICAL UNIVERSITY
发明人： Guoqiang TAN , Jianghui LI , Feng LIANG , Yilin PANG
IPC分类号： C12Q1/48 , C12N15/70 , C12N9/10
CPC分类号： C12Q1/48 , C12N15/70 , C12Y208/01007 , C12N9/13 , C12N2800/101
摘要： The present disclosure provides a method for detecting L-serine based on cysteine desulfurase-containing living Escherichia coli cells, and belongs to the technical field of amino acid detection. The method includes the following steps: incubating an unknown sample with the cysteine desulfurase-containing living E. coli cells to produce a red substance, and qualitatively or semi-quantitatively detecting L-serine content in the unknown sample according to color changes of the red substance of the living E. coli cells, or quantitatively detecting L-serine content in the unknown sample by measuring absorbance of a lysate of the living E. coli cells. The detection method provided by the present disclosure is simple and convenient in process, few in reaction steps and stable in enzymatic activity of living cells.

9. 发明授权

US10612010B2 Thermophile-derived keratinase and use thereof 有权
公开(公告)号：US10612010B2
公开(公告)日：2020-04-07
申请号：US15509095
申请日：2015-09-07
申请人： KYUNGPOOK NATIONAL UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION
发明人： Dong Woo Lee , Yong Jik Lee , Hyun Su Jin , Ji Yeon Kim , Gae Won Nam , Sang Jae Lee
IPC分类号： C12N9/00 , C12N15/52 , C12N9/48 , C12N9/10 , C12N9/02
CPC分类号： C12N9/13 , C12N9/00 , C12N9/0051 , C12N9/48 , C12N9/485 , C12N15/52 , C12Y108/01009 , C12Y208/01007 , C12Y304/16005
摘要： The present invention relates to a novel thermophile-derived keratinase having keratin decomposition ability. Further, the present invention relates to a polynucleotide encoding the keratinase, a recombinant vector containing the same, host cells transformed by using the recombinant vector, and a method for preparing a keratinase including a step of culturing the host cells. Further, the present invention relates to a composition for decomposing keratin containing the enzyme; and a method for decomposing keratin by using the same. Further, the present invention relates to a keratin decomposed product decomposed by the enzyme.
The keratinase according to the present invention rapidly and effectively decomposes hardly-decomposable keratin, and thus it is expected that the keratinase can be used for the effective treatment and the high value-added resource recovery of agricultural and livestock waste, which causes environmental problems (for example, a novel material for enzyme cosmetics), and can be used in an innovative enzymatic bioconversion technique utilizing various decomposition enzyme groups.

10. 发明申请

US20170321235A1 TRANSFORMED FUNGUS HAVING ENHANCED ERGOTHIONEINE PRODUCTIVITY AND METHOD FOR PRODUCING ERGOTHIONEINE 审中-公开
公开(公告)号：US20170321235A1
公开(公告)日：2017-11-09
申请号：US15533215
申请日：2015-12-25
申请人： KIKKOMAN CORPORATION
发明人： Seiichi HARA , Keiko KUROSAWA , Keiichi ICHIKAWA
IPC分类号： C12P13/04 , C12N9/10
CPC分类号： C12P13/04 , C12N9/10 , C12N9/1007 , C12N9/13 , C12N15/09 , C12R1/66 , C12R1/665 , C12R1/685 , C12R1/69 , C12Y201/01 , C12Y208/01007
摘要： The purpose of the present invention is to provide an organism having an ergothioneine productivity that is capable of easily producing ergothioneine within a short period of time at a high yield, as compared with a conventional technology, and, therefore, enables ergothioneine production on an industrial scale. This purpose can be achieved by a transformed fungus into which a gene encoding enzyme (1) or genes encoding enzymes (1) and (2) have been inserted and in which the inserted gene(s) are overexpressed. (1) an enzyme catalyzing a reaction of synthesizing hercynyl cysteine sulfoxide from histidine and cysteine in the presence of S-adenosyl methionine, iron (II) and oxygen. (2) An enzyme catalyzing a reaction of synthesizing ergothioneine from hercynyl cysteine sulfoxide using pyridoxal 5′-phosphate as a coenzyme.

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