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1. 发明申请

US20110081318A1 VIRAL COMPOSITIONS AND METHODS 审中-公开
标题翻译：病毒组合物和方法
公开(公告)号：US20110081318A1
公开(公告)日：2011-04-07
申请号：US12863351
申请日：2009-01-16
申请人： Asaf Korkut
发明人： Asaf Korkut
IPC分类号： A61K35/76 , C12N7/01 , A61P43/00
CPC分类号： C12N7/00 , C12N2740/16051 , C12N2760/12151 , C12N2760/14151 , C12N2760/16151 , C12N2760/20151 , C12N2770/24151 , C12N2770/24251
摘要： Methods for producing recombinant viruses, compositions including the viruses, and uses for the compositions are described herein.
摘要翻译：本文描述了用于产生重组病毒的方法，包括病毒的组合物和组合物的用途。

2. 发明申请

US20130203152A1 Method for Reducing DNA Impurities in Viral Compositions 有权
标题翻译：减少病毒组合物中DNA杂质的方法
公开(公告)号：US20130203152A1
公开(公告)日：2013-08-08
申请号：US13813897
申请日：2010-08-12
申请人： Yi Zhang , Jinming Dai , Yajin Ni
发明人： Yi Zhang , Jinming Dai , Yajin Ni
IPC分类号： C12N7/02 , C12N7/06
CPC分类号： C12N7/02 , A61K39/12 , A61K2039/525 , C12N7/00 , C12N7/06 , C12N2760/20134 , C12N2760/20151
摘要： A method for purification of viral compositions is provided. In particular, a method for reduction of unwanted residual DNA in a viral composition while retaining the immunogenicity of the virus itself is provided. The resulting immunogenic viral composition is substantially free of residual DNA, and is useful for the manufacture of medical products, such as vaccines designed for human or animal.
摘要翻译：提供了病毒组合物的纯化方法。具体地，提供了在保持病毒本身的免疫原性的同时还原病毒组合物中不需要的残留DNA的方法。所得的免疫原性病毒组合物基本上不含残留的DNA，并且可用于制造医疗产品，例如为人或动物设计的疫苗。

3. 发明申请

US20050260601A1 Recombinant mutants of rhabdovirus and methods of use thereof 审中-公开
标题翻译：弹状病毒的重组突变体及其使用方法
公开(公告)号：US20050260601A1
公开(公告)日：2005-11-24
申请号：US10656894
申请日：2003-09-08
申请人： Michael Whitt , Clinton Robison , Himangi Jayakar , Mark Miller
发明人： Michael Whitt , Clinton Robison , Himangi Jayakar , Mark Miller
IPC分类号： C12N15/09 , A61K31/7088 , A61K35/76 , A61K35/766 , A61K39/00 , A61K39/07 , A61K48/00 , A61P1/16 , A61P3/10 , A61P5/14 , A61P9/00 , A61P9/12 , A61P11/00 , A61P11/06 , A61P13/12 , A61P17/00 , A61P17/02 , A61P17/06 , A61P19/02 , A61P19/10 , A61P21/04 , A61P25/00 , A61P25/14 , A61P25/16 , A61P25/18 , A61P25/24 , A61P25/28 , A61P27/02 , A61P27/16 , A61P29/00 , A61P31/04 , A61P35/00 , A61P37/04 , A61P37/06 , C07K14/145 , C12N7/00 , C12N7/04 , C12N15/86 , C12Q1/02 , C12Q1/68 , G01N33/50 , G01N33/574
CPC分类号： C07K14/005 , A61K35/766 , A61K38/1709 , A61K38/2013 , A61K38/208 , A61K38/21 , A61K39/00 , A61K39/07 , A61K48/00 , A61K2039/5254 , A61K2039/5256 , A61K2039/55538 , C07K2319/00 , C12N7/00 , C12N15/86 , C12N2760/20122 , C12N2760/20143 , C12N2760/20151 , C12N2760/20222 , C12N2760/20232 , C12N2760/20261 , C12N2810/60 , C12N2840/20 , G01N33/5011 , A61K2300/00
摘要： The present invention relates to recombinant Rhabdoviridae, isolated nucleic acids, vectors, cells and compositions comprising same. The recombinant Rhabdoviridae, isolated nucleic acids, vectors, cells and compositions express Rhabdoviral proteins including a mutated matrix protein (M) and/or a mutated glycoprotein (G), in addition to expression of at least one foreign nucleic acid. The present invention also relates to methods of use thereof, including their use in vivo, in anti-cancer applications, such as in the treatment of gliomas. The recombinant Rhabdoviridae of the present invention are also useful in gene therapy and vaccine applications.
摘要翻译：本发明涉及重组弹状病毒科，分离的核酸，载体，细胞和包含其的组合物。除了表达至少一种外源核酸外，重组的弹状病毒科，分离的核酸，载体，细胞和组合物还表达包括突变的基质蛋白（M）和/或突变的糖蛋白（G）的Rhabdoviral蛋白质。本发明还涉及其用途的方法，包括它们在体内的用途，在抗癌应用中，例如用于治疗神经胶质瘤。本发明的重组革兰氏病毒杆菌科也可用于基因治疗和疫苗应用。

4. 发明授权

US4255520A Process for the preparation of a rabies vaccine and vaccine obtained by this process 失效
标题翻译：通过该方法获得的狂犬病疫苗和疫苗的制备方法
公开(公告)号：US4255520A
公开(公告)日：1981-03-10
申请号：US136722
申请日：1980-04-02
申请人： Klaus R. Schell
发明人： Klaus R. Schell
IPC分类号： A61K39/205
CPC分类号： A61K39/205 , A61K39/12 , C12N7/00 , A61K2039/5252 , C12N2760/20134 , C12N2760/20151
摘要： A method for preparing a rabies vaccine wherein rabies viruses are multiplied in poultry embryos, the heads of the embryos are harvested and the cell extract containing the rabies antigens is separated therefrom for differential centrifugation and density gradient centrifugation, the separated antigen is further processed using conventional methods. This process results in a highly selective concentration of antigens and the production of viruses having a very high activity and freedom from contaminating proteins.
摘要翻译：制备狂犬病疫苗的方法，其中狂犬病病毒在家禽胚胎中繁殖，收获胚胎的头部，从其中分离出含有狂犬病抗原的细胞提取物用于差速离心和密度梯度离心，使用常规方法进一步处理分离的抗原方法。该方法导致抗原的高选择性浓度和具有非常高的活性并且不受污染蛋白质的病毒的产生。

5. 发明申请

US20110151506A1 Process for Culturing Adherent Cells 有权
标题翻译：培养粘附细胞的方法
公开(公告)号：US20110151506A1
公开(公告)日：2011-06-23
申请号：US12976679
申请日：2010-12-22
申请人： Eric Calvosa , Nicolas Seve
发明人： Eric Calvosa , Nicolas Seve
IPC分类号： C12P1/00 , C12N5/10
CPC分类号： C12N5/00 , C12N7/00 , C12N2500/92 , C12N2500/99 , C12N2760/20151
摘要： The subject of the present invention is a process for producing adherent cells comprising: a. introducing a suspension of adherent cells into a culture vessel containing microcarriers in a culture medium; b. amplifying the cells by performing a plurality of cell passages in the same culture vessel wherein each cell passage subsequent to the first is carried out: i) by using all or part of the cells produced during the previous cell passage after having subjected the cells to enzyme treatment to detach the cells from the microcarriers, and ii) by introducing culture medium and an increasing amount of microcarriers into the culture medium; and c. harvesting the cells produced during the last cell passage, optionally after having subjected the cells to enzyme treatment to detach cells from microcarriers. The invention also relates to the implementation of this process for the production of biological agents, serving in particular to prepare vaccines or drugs.
摘要翻译：本发明的主题是制备贴壁细胞的方法，包括：a。将粘附细胞的悬浮液引入培养基中含有微载体的培养容器中; b。通过在相同的培养容器中进行多个细胞通道来扩增细胞，其中进行第一次之后的每个细胞通道：i）通过在使细胞进入酶之后使用在先前细胞传代期间产生的全部或部分细胞处理以从微载体中分离细胞，和ii）通过将培养基和增加量的微载体导入培养基中; 和c。收集在最后一个细胞传代期间产生的细胞，任选地在使细胞进行酶处理以从微载体分离细胞之后。本发明还涉及用于生产生物制剂的该方法的实施，特别是用于制备疫苗或药物。

6. 发明授权

US4206014A Process for removing detergents from virus-antigen suspensions 失效
标题翻译：从病毒 - 抗原悬浮液中去除洗涤剂的方法
公开(公告)号：US4206014A
公开(公告)日：1980-06-03
申请号：US958512
申请日：1978-11-07
申请人： Edgar Reichert , Mirko Majer
发明人： Edgar Reichert , Mirko Majer
IPC分类号： C12N7/00 , A61K39/12 , A61K39/145 , A61K39/205 , C12N7/06 , A61K39/18 , A61K39/28
CPC分类号： C12N7/00 , A61K39/12 , A61K39/145 , A61K39/205 , C12N2760/16134 , C12N2760/16151 , C12N2760/16234 , C12N2760/16251 , C12N2760/18834 , C12N2760/18851 , C12N2760/20134 , C12N2760/20151 , Y10S435/961 , Y10S530/826
摘要： A method for isolating a detergent-free virus-antigen from a suspension of said virus-antigen containing detergent, which method comprises precipitating said virus-antigen by adding a polyethylene glycol to said suspension and then isolating the detergent-free virus-antigen precipitate by sedimentation, said detergent remaining in said suspension.
摘要翻译：一种从所述含有病毒抗原的洗涤剂的悬浮液中分离无去污剂的病毒抗原的方法，该方法包括通过向所述悬浮液中加入聚乙二醇使所述病毒抗原沉淀，然后通过所述洗涤剂沉淀在所述悬浮液中。

7. 发明授权

US4071619A Method of producing vaccines 失效
标题翻译：生产疫苗的方法
公开(公告)号：US4071619A
公开(公告)日：1978-01-31
申请号：US719692
申请日：1976-09-02
申请人： Tamaz Vladimirovich Peradze , Eidlya Abramovna Fridman , Nina Vsevolodovna Zheleznova , Vsevolod Mikhailovich Kolikov , Semen Khaimovich-Efimovich Bresler , Boris Viktorovich Mchedlishvili , Viktor Mikhailovich Molodkin , Ljudmila Mikhailovna Molodkina , Nina Vasilievna Katushkina
发明人： Tamaz Vladimirovich Peradze , Eidlya Abramovna Fridman , Nina Vsevolodovna Zheleznova , Vsevolod Mikhailovich Kolikov , Semen Khaimovich-Efimovich Bresler , Boris Viktorovich Mchedlishvili , Viktor Mikhailovich Molodkin , Ljudmila Mikhailovna Molodkina , Nina Vasilievna Katushkina
IPC分类号： A61K39/12 , A61K39/13 , A61K39/145 , A61K39/205 , A61K41/00 , C12K7/00
CPC分类号： A61K39/12 , A61K39/13 , A61K39/145 , A61K39/205 , C12N7/00 , A61K2039/5252 , C12N2760/10034 , C12N2760/10051 , C12N2760/16134 , C12N2760/16151 , C12N2760/20134 , C12N2760/20151
摘要： A method for producing vaccines wherein a suspension of the viral culture is purified by gel chromatography in a column packed with a porous virion non-sorbing silicate material having pore diameter of 50 to 10000 A, followed by adsorption-elution concentration and completion of the vaccine, or the starting suspension of the viral culture is concentrated by adsorption-elution and then purified by gel chromatography in a column packed with porous silicate material having pore diameter of 50 to 10000 A, or the starting suspension of the viral culture is subjected to gel chromatography in a column filled with a porous silicate material having pore diameter of 50 to 10000 A and the final vaccine is produced.
摘要翻译：一种生产疫苗的方法，其中病毒培养物的悬浮液通过凝胶色谱法在填充有孔径为50至10000A的多孔病毒粒子非吸附硅酸盐材料的柱中进行纯化，然后进行吸附洗脱浓度和疫苗完成，或通过吸附洗脱浓缩病毒培养物的起始悬浮液，然后通过凝胶色谱法在填充有孔径为50至10000的多孔硅酸盐材料的柱中纯化，或将病毒培养物的起始悬浮液进行凝胶在填充有孔径为50至10000的多孔硅酸盐材料的柱中进行色谱法，并制备最终疫苗。

8. 发明授权

US2879202A Stabilization of live viral vaccines by hexahydric alcohols 失效
标题翻译：通过六元醇稳定活病毒疫苗
公开(公告)号：US2879202A
公开(公告)日：1959-03-24
申请号：US46721354
申请日：1954-11-05
申请人： AMERICAN CYANAMID CO
发明人： STEWART AISTON , DANIELSON IRVIN S
IPC分类号： A61K39/12 , A61K39/13 , A61K39/205 , A61K39/275
CPC分类号： A61K39/13 , A61K9/0019 , A61K39/12 , A61K39/205 , A61K39/275 , A61K47/26 , A61K2039/5254 , C12N2710/24134 , C12N2710/24151 , C12N2760/20134 , C12N2760/20151 , C12N2770/32634 , C12N2770/32651

9. 发明授权

US09750800B2 Method for purifying the rabies virus 有权
公开(公告)号：US09750800B2
公开(公告)日：2017-09-05
申请号：US14593337
申请日：2015-01-09
申请人： Virginie Fabre , Celine Rocca , Pierre Riffard , Eric Calvosa
发明人： Virginie Fabre , Celine Rocca , Pierre Riffard , Eric Calvosa
IPC分类号： A61K39/205 , C12N7/00 , A61K39/12
CPC分类号： A61K39/205 , A61K39/12 , C12N7/00 , C12N2760/20134 , C12N2760/20151
摘要： The subject of the invention is a method for purifying the rabies virus, comprising a single ion-exchange chromatography step, said step being cation exchange chromatography according to which: a) the supernatant of a culture of cells infected with this virus is brought into contact with a cation exchange chromatography support comprising a polymethacrylate matrix onto which sulfoisobutyl groups have been grafted such that the rabies virus binds to this support, and; b) the virus is eluted from its support.

10. 发明授权

US09145543B2 Process for culturing adherent cells 有权
标题翻译：培养贴壁细胞的方法
公开(公告)号：US09145543B2
公开(公告)日：2015-09-29
申请号：US12976679
申请日：2010-12-22
申请人： Eric Calvosa , Nicolas Seve
发明人： Eric Calvosa , Nicolas Seve
IPC分类号： C12P1/00 , C12N1/00 , C12N5/00 , C12N7/00
CPC分类号： C12N5/00 , C12N7/00 , C12N2500/92 , C12N2500/99 , C12N2760/20151
摘要： The subject of the present invention is a process for producing adherent cells comprising: a. introducing a suspension of adherent cells into a culture vessel containing microcarriers in a culture medium; b. amplifying the cells by performing a plurality of cell passages in the same culture vessel wherein each cell passage subsequent to the first is carried out: i) by using all or part of the cells produced during the previous cell passage after having subjected the cells to enzyme treatment to detach the cells from the microcarriers, and ii) by introducing culture medium and an increasing amount of microcarriers into the culture medium; and c. harvesting the cells produced during the last cell passage, optionally after having subjected the cells to enzyme treatment to detach cells from microcarriers. The invention also relates to the implementation of this process for the production of biological agents, serving in particular to prepare vaccines or drugs.
摘要翻译：本发明的主题是制备贴壁细胞的方法，包括：a。将粘附细胞的悬浮液引入培养基中含微载体的培养容器中; b。通过在相同的培养容器中进行多个细胞通道来扩增细胞，其中进行第一次之后的每个细胞通道：i）通过在使细胞进入酶之后使用在先前细胞传代期间产生的全部或部分细胞处理以从微载体中分离细胞，和ii）通过将培养基和增加量的微载体导入培养基中; 和c。收集在最后一个细胞传代期间产生的细胞，任选地在使细胞进行酶处理以从微载体分离细胞之后。本发明还涉及用于生产生物制剂的该方法的实施，特别是用于制备疫苗或药物。

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