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    • 10. 发明申请
    • METHOD FOR PRODUCTION OF HIGHLY PURE PHOSPHOLIPID, AND HIGHLY PURE SPHINGOMYELIN AND PLASMALOGEN-TYPE GLYCEROPHOSPHOLIPID PRODUCED BY THE METHOD
    • 生产高纯度磷脂的方法,以及通过该方法生产的高纯度磷脂酰胆碱和等离子体型糖苷磷酸酯
    • US20110160471A1
    • 2011-06-30
    • US12999801
    • 2009-06-19
    • Yoshitaka NadachiSiro Mawatari
    • Yoshitaka NadachiSiro Mawatari
    • C07F9/24C12P9/00C07F9/10
    • C11B1/10A23J7/00A23L33/115C11B11/00C11B13/00Y02W30/74
    • This invention provides a process for producing a high-purity sphingomyelin and a high-purity plasmalogen-form glycerophospholipid from a biological material by simple procedures at high yields. The process comprises the steps of: (A) subjecting dried total lipids extracted from a biological material to extraction treatment with a specific mixture solution to separate an insoluble portion composed mainly of sphingomyelin and a soluble portion; (B) subjecting the insoluble portion, obtained in said Step (A), to washing treatment with a specific mixture solution to obtain crude sphingomyelin; (C) subjecting the soluble portion, obtained in said Step (A), to washing treatment with a water-soluble ketone solvent to obtain crude plasmalogen-form glycerophospholipid; (D) causing an enzyme to act on the crude sphingomyelin, obtained in said Step (B), to obtain sphingomyelin having a purity of 90% or more; and (E) causing an enzyme to act on the crude plasmalogen-form glycerophospholipid, obtained in said Step (C), to obtain plasmalogen-form glycerophospholipid having a purity of 40% or more.
    • 本发明提供了一种以高产率通过简单的方法从生物材料制备高纯度鞘磷脂和高纯度血浆原型形成的甘油磷脂的方法。 该方法包括以下步骤:(A)使用生物材料提取的干燥的总脂质用特定的混合溶液进行提取处理,以分离主要由鞘磷脂和可溶部分组成的不溶部分; (B)将所述工序(A)得到的不溶部分用特定的混合溶液洗涤,得到粗的鞘磷脂; (C)将所述工序(A)得到的可溶部分用水溶性酮溶剂进行洗涤处理,得到粗制的原浆形式的甘油磷脂; (D)使所述步骤(B)中得到的粗制鞘磷脂作用于酶,得到纯度为90%以上的鞘磷脂; 和(E)使酶作用于所述步骤(C)中获得的粗制浆素形式的甘油磷脂,以获得纯度为40%以上的浆果形式的甘油磷脂。