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    • 10. 发明授权
    • Tissue engineered cartilage, method of making same, therapeutic and cosmetic surgical applications using same
    • 组织工程软骨,其制备方法,使用其的治疗和美容手术应用
    • US08202551B2
    • 2012-06-19
    • US11922251
    • 2006-06-15
    • Wan-Ju LiRocky S. Tuan
    • Wan-Ju LiRocky S. Tuan
    • A01N65/00
    • A61Q90/00A61L27/18A61L27/3817A61L27/3852A61L27/3895A61L27/56A61L2400/12A61L2430/06C08L67/04
    • Cartilage has been constructed using biodegradable electrospun polymeric scaffolds seeded with chondrocytes or adult mesenchymal stem cells. More particularly engineered cartilage has been prepared where the cartilage has a biodegradable and biocompatible nanofibrous polymer support prepared by electrospinning and a plurality of chondocytes or mesenchymal stem cells dispersed in the pores of the support. The tissue engineered cartilages of the invention possess compressive strength properties similar to natural cartilage. Methods of preparing engineered tissues, including tissue engineered cartilages, are provided in which an electrospun nanofibrous polymer support is provided, the support is treated with a cell solution and the polymer-cell mixture cultured in a rotating bioreactor to generate the cartilage. The invention provides for the use of the tissue engineered cartilages in the treatment of cartilage degenerative diseases, reconstructive surgery, and cosmetic surgery.
    • 软骨已经使用生物降解的电纺聚合物支架构建,该支架用软骨细胞或成体间充质干细胞接种。 已经制备了更特别工程化的软骨,其中软骨具有通过静电纺丝制备的可生物降解和生物相容的纳米纤维聚合物载体和分散在载体孔中的多个软骨细胞或间充质干细胞。 本发明的组织工程软骨具有类似于天然软骨的抗压强度性质。 提供了制备工程组织,包括组织工程软骨的方法,其中提供电纺丝纳米纤维聚合物载体,将载体用细胞溶液处理,并将聚合物 - 细胞混合物在旋转的生物反应器中培养以产生软骨。 本发明提供组织工程软骨在治疗软骨退行性疾病,重建手术和整容手术中的应用。