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1. 发明授权

US07313482B2 Selection of target sites for antisense attack of RNA 失效
标题翻译：选择RNA反义突变的靶位点
公开(公告)号：US07313482B2
公开(公告)日：2007-12-25
申请号：US10478049
申请日：2002-05-15
申请人： Karl Drlica , Jian-Ying Wang
发明人： Karl Drlica , Jian-Ying Wang
IPC分类号： G01N33/48 , G01N33/00 , C12Q1/00 , C12Q1/68
CPC分类号： C12N15/113 , G06F19/18
摘要： Nucleic acid hybridization under steady-state conditions is described by a kinetic model in which the intermediate state is assumed to be locally single stranded. An expression was derived that relates nucleic acid secondary structure to the rate of oligonucleotide-RNA hybridization. The model allows the calculation of a rate factor that is proportional to the rate constant for hybridization between complementary nucleic acids and is generally applicable to any RNA molecule with potential utility for rapid identification of sites for antisense attack of mRNA.
摘要翻译：在稳态条件下的核酸杂交通过动力学模型描述，其中假定中间状态是局部单链的。得出了将核酸二级结构与寡核苷酸 - RNA杂交速率相关联的表达。该模型允许计算与互补核酸之间杂交的速率常数成比例的速率因子，并且通常适用于具有用于快速鉴定mRNA反义位点的潜在效用的任何RNA分子。

2. 发明授权

US07129087B2 Oligonucleotide-facilitated coalescence 有权
标题翻译：寡核苷酸促进聚结
公开(公告)号：US07129087B2
公开(公告)日：2006-10-31
申请号：US10398833
申请日：2001-10-11
申请人： Fred R. Kramer , Osama A. Alsmadi , Sanjay Tyagi
发明人： Fred R. Kramer , Osama A. Alsmadi , Sanjay Tyagi
IPC分类号： C12N15/02 , C12N15/88 , A61K9/127
CPC分类号： A61K9/127 , A61K9/1271
摘要： Coalescence of cells or other membrane-bound entities is facilitated by anchoring an outwardly projecting first oligonucleotide in one member and an outwardly projecting second oligonucleotide, complementary to the first, in a second member and incubating under hybridizing conditions. Liposomes may be coalesced with cells to deliver hydrophilic agents thereto, such as DNA probes or drugs. Kits containing complementary oligonucleotides containing hydrophobic anchoring moieties may be used.
摘要翻译：通过将向外突出的第一寡核苷酸锚定在一个构件中并通过在第二构件中与第一寡核苷酸互补的向外突出的第二寡核苷酸并在杂交条件下孵育来促进细胞或其它膜结合实体的聚结。脂质体可以与细胞聚结以向其递送亲水剂，例如DNA探针或药物。可以使用含有含有疏水性锚定部分的互补寡核苷酸的试剂盒。

3. 发明申请

US20060240566A1 Delivery of metered amounts of liquid materials 审中-公开
标题翻译：交付量的液体材料
公开(公告)号：US20060240566A1
公开(公告)日：2006-10-26
申请号：US11471889
申请日：2006-06-20
申请人： Timothy Chang , Peter Tolias
发明人： Timothy Chang , Peter Tolias
IPC分类号： B01L3/00
CPC分类号： G01N35/1074 , B01J2219/00387 , B01L2400/028 , C40B60/14 , G01N35/1002 , G01N35/1016 , G01N2035/1037 , G01N2035/1039 , Y10T436/2575
摘要： Techniques for the delivery of metered amounts of liquid material include the use of a pin, such as an optical fiber. A sample of the liquid material may be placed on the tip of the pin for delivery to a target area, such as a specific location on a microarray, in a contactless manner. Light transmitted through the pin may be detected to facilitate accurate measuring of the pin's position.
摘要翻译：用于递送计量的液体材料的技术包括使用诸如光纤的销。可以将液体材料的样品放置在针的尖端上，以非接触的方式传送到目标区域，例如微阵列上的特定位置。可以检测透过销的光线，以便于精确测量销的位置。

4. 发明授权

US6103463A Method of sorting a mixture of nucleic acid strands on a binary array 失效
标题翻译：在二进制阵列上排列核酸链的混合物的方法
公开(公告)号：US6103463A
公开(公告)日：2000-08-15
申请号：US247530
申请日：1994-05-23
申请人： Alexander B. Chetverin , Fred Russell Kramer
发明人： Alexander B. Chetverin , Fred Russell Kramer
IPC分类号： B01J19/00 , C12M1/34 , C12N15/10 , C12Q1/68 , C40B40/06 , C40B50/14 , C40B60/14
CPC分类号： B82Y30/00 , B01J19/0046 , C12N15/10 , C12Q1/6806 , C12Q1/6811 , C12Q1/6834 , C12Q1/6837 , C12Q1/6853 , C12Q1/686 , C12Q1/6874 , B01J2219/00313 , B01J2219/00315 , B01J2219/00527 , B01J2219/00529 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00621 , B01J2219/00626 , B01J2219/00637 , B01J2219/00644 , B01J2219/00659 , B01J2219/00662 , B01J2219/00675 , B01J2219/00722 , C40B40/06 , C40B50/14 , C40B60/14 , Y10S435/81 , Y10S436/808
摘要： A method of sorting mixtures of nucleic acid strands comprising hybridizing the strands to an array of immobilized oligonucleotides, each of which includes a constant segment adjacent to a variable segment. The constant segment of the immobilized oligonucleotides can be made complementary to the ends of strands obtained by digesting a double-stranded nucleic acid with a restriction enzyme and restoring the restriction sites, thereby permitting the sorting of strands according to their variable sequences adjacent to their constant terminal restored restriction sites.
摘要翻译：一种分选核酸链的混合物的方法，其包括将所述链杂交至固定化寡核苷酸阵列，其中每一个包括与可变区相邻的恒定区。可以使固定的寡核苷酸的恒定区域与通过用限制酶消化双链核酸获得的链的末端互补，并恢复限制性位点，从而允许根据它们与其常数相邻的可变序列对链进行分选终端恢复限制站点。

5. 发明授权

US5925517A Detectably labeled dual conformation oligonucleotide probes, assays and kits 失效
标题翻译：可检测标记的双构型寡核苷酸探针，测定和试剂盒
公开(公告)号：US5925517A
公开(公告)日：1999-07-20
申请号：US439819
申请日：1995-05-12
申请人： Sanjay Tyagi , Fred R. Kramer , Paul M. Lizardi
发明人： Sanjay Tyagi , Fred R. Kramer , Paul M. Lizardi
IPC分类号： C12N15/09 , C07H21/00 , C07H21/04 , C12Q1/68 , C07H21/02
CPC分类号： C12Q1/6816 , C12Q1/6818 , C12Q1/6827 , C12Q1/6841
摘要： Unimolecular and bimolecular hybridization probes for the detection of nucleic acid target sequences comprise a target complement sequence, an affinity pair holding the probe in a closed conformation in the absence of target sequence, and either a label pair that interacts when the probe is in the closed conformation or, for certain unimolecular probes, a non-interactive label. Hybridization of the target and target complement sequences shifts the probe to an open conformation. The shift is detectable due to reduced interaction of the label pair or by detecting a signal from a non-interactive label. Certain unimolecular probes can discriminate between target and non-target sequences differing by as little as one nucleotide. Also, universal stems and kits useful for constructing said probes. Also, assays utilizing said probes and kits for performing such assays.
摘要翻译：用于检测核酸靶序列的单分子和双分子杂交探针包含靶补体序列，在不存在靶序列的情况下将探针保持在封闭构象中的亲和对，以及当探针处于封闭状态时相互作用的标记对构象，或对于某些单分子探针，非交互式标记。靶和补体序列的杂交将探针转移到开放构象。由于标签对的相互作用减少或通过检测来自非交互式标签的信号，该位移是可检测的。某些单分子探针可以区分不同于一个核苷酸的靶和非靶序列。此外，通用的茎和试剂盒可用于构建所述探针。此外，利用所述探针和试剂盒进行这种测定的测定。

6. 发明授权

US5643756A Fusion glycoproteins 失效
标题翻译：融合糖蛋白
公开(公告)号：US5643756A
公开(公告)日：1997-07-01
申请号：US110300
申请日：1993-08-20
申请人： Samuel Kayman , Abraham Pinter
发明人： Samuel Kayman , Abraham Pinter
IPC分类号： C07K14/15 , C07K14/16 , C12N15/867 , C12N5/10 , C12N15/85 , C12P21/02
CPC分类号： C07K14/005 , C12N15/86 , C07K2319/00 , C12N2740/13022 , C12N2740/13043 , C12N2740/13045 , C12N2740/16043 , C12N2740/16045 , C12N2740/16222 , C12N2810/60 , C12N2810/6054
摘要： Novel expression vectors are provided for expressing a fusion glycoprotein. The fusion glycoprotein contains the N-terminal globular domain of a retroviral env surface protein linked to a selected glycopeptide. Truncation glycoproteins as well as insertion glycoproteins are expressed using the vectors.
摘要翻译：提供新的表达载体用于表达融合糖蛋白。融合糖蛋白含有与所选糖肽连接的逆转录病毒env表面蛋白的N末端球状结构域。使用载体表达截断糖蛋白以及插入糖蛋白。

7. 发明申请

US20100120048A1 ASSAYS FOR SHORT SEQUENCE VARIANTS 审中-公开
标题翻译：短期序列变量的测定
公开(公告)号：US20100120048A1
公开(公告)日：2010-05-13
申请号：US12614917
申请日：2009-11-09
申请人： Sanjay Tyagi , Fred Kramer , David Alland
发明人： Sanjay Tyagi , Fred Kramer , David Alland
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689 , C12Q1/6818 , C12Q2525/301
摘要： The invention provides assays that can detect multiple genetic variants of a gene (e.g., a mycobacterium gene) in a sample using a pool (using 2, 3, 4, or more) of oligonucletide hybridization probes.
摘要翻译：本发明提供了使用池（使用2,3,4或更多）寡核苷酸杂交探针在样品中检测基因（例如分枝杆菌基因）的多种遗传变体的测定法。

8. 发明授权

US5759773A Sensitive nucleic acid sandwich hybridization assay 失效
标题翻译：敏感核酸夹心杂交测定
公开(公告)号：US5759773A
公开(公告)日：1998-06-02
申请号：US393888
申请日：1995-02-24
申请人： Sanjay Tyagi , Fred R. Kramer , Paul M. Lizardi , Ulf D. Landegren , Herman J. Blok
发明人： Sanjay Tyagi , Fred R. Kramer , Paul M. Lizardi , Ulf D. Landegren , Herman J. Blok
IPC分类号： G01N33/53 , C07H21/04 , C12N15/09 , C12Q1/34 , C12Q1/68 , G01N33/566 , G01N33/58 , C12P19/34
CPC分类号： C12Q1/6867 , C12Q1/6813 , C12Q1/682 , C12Q1/6823 , C12Q1/6853 , C12Q1/686 , C12Q1/6862
摘要： Nucleic acid sandwich hybridization assays are provided that incorporate one or a combination of background reduction steps. Those steps include use of a separate capture probe and separation from immobilized capture probes by cleavage and isolation. A very sensitive assay for RNA targets includes both of those steps, plus RNA binary probes, an RNA-directed RNA ligase and amplification by an RNA-directed RNA polymerase. Kits of reagents for performing assays according to this invention are also provided.
摘要翻译：提供核酸夹心杂交测定法，其包含背景还原步骤的一种或组合。那些步骤包括使用单独的捕获探针，并通过裂解和分离与固定的捕获探针分离。对RNA靶标的非常敏感的测定包括这两个步骤，加上RNA二元探针，RNA指导的RNA连接酶和通过RNA指导的RNA聚合酶的扩增。还提供了用于进行根据本发明的测定的试剂盒。

9. 发明申请

US20110129828A1 SPECIFIC DOUBLE-STRANDED PROBES FOR HOMOGENEOUS DETECTION OF NUCLEIC ACID AND THEIR APPLICATION METHODS 有权
标题翻译：用于均质检测核酸的特异性双重探针及其应用方法
公开(公告)号：US20110129828A1
公开(公告)日：2011-06-02
申请号：US12854619
申请日：2010-08-11
申请人： Qingge Li , Jixuan Liang , Guoyan Luan
发明人： Qingge Li , Jixuan Liang , Guoyan Luan
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6813 , C07H21/00 , C07H21/04 , C12Q1/6818 , C12Q1/6844 , C12Q2565/525 , C12Q2563/107 , C12Q2561/113 , C12Q2537/161 , C12Q2525/204
摘要： Nucleic acid detection probes that comprise a pair of complementary, fluorophore/quencher labeled oligonucleotides, one of which is shorter than the other, are able to detect single-stranded and double-stranded targets in hybridization reactions and amplification reactions with real-time detection. Double-stranded probes of equal length are useful in PCR amplification reactions with real-time detection.
摘要翻译：包含一对互补的荧光团/猝灭剂标记寡核苷酸的核酸检测探针，其中之一短于另一个，能够通过实时检测在杂交反应和扩增反应中检测单链和双链靶标。具有相等长度的双链探针可用于具有实时检测的PCR扩增反应。

10. 发明授权

US5652107A Diagnostic assays and kits for RNA using RNA binary probes and a ribozyme ligase 失效
标题翻译：使用RNA二元探针和核酶连接酶的RNA的诊断测定和试剂盒
公开(公告)号：US5652107A
公开(公告)日：1997-07-29
申请号：US683045
申请日：1996-07-15
申请人： Paul M. Lizardi , Sanjay Tyagi , Ulf D. Landegren , Fred R. Kramer , Jack W. Szostak
发明人： Paul M. Lizardi , Sanjay Tyagi , Ulf D. Landegren , Fred R. Kramer , Jack W. Szostak
IPC分类号： C12N15/09 , C07H21/02 , C12Q1/68 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/6867 , C12Q1/682 , C12Q1/6862
摘要： There are provided nucleic acid hybridization assays for RNA targets using RNA binary probes and a ribozyme ligase that is a stringent RNA-directed RNA ligase. Preferred assays include exponential amplification for signal generation. Tetrahymena ribozyme ligase is a preferred ligase for use in this invention. It may be tethered to hold it close to the ligation junction. One assay according to this invention is a "tethered ligase chain reaction." Also provided are kits for performing assays according to the invention.
摘要翻译：使用RNA二元探针和作为严格的RNA指导的RNA连接酶的核酶连接酶为RNA靶提供核酸杂交测定。优选的测定包括用于信号产生的指数扩增。四膜虫核酶连接酶是用于本发明的优选连接酶。它可能被束缚以使其靠近结扎处。根据本发明的一种测定法是“连接连接酶链反应”。还提供了用于进行根据本发明的测定的试剂盒。

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