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1. 发明申请

US20110021361A1 Novel oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids 审中-公开
标题翻译：新型寡核苷酸阵列及其用于分选，分离，测序和操作核酸的用途
公开(公告)号：US20110021361A1
公开(公告)日：2011-01-27
申请号：US12755287
申请日：2010-04-06
申请人： Alexander B. Chetverin , Fred Russell Kramer
发明人： Alexander B. Chetverin , Fred Russell Kramer
IPC分类号： C40B30/00 , C12Q1/68
CPC分类号： B82Y30/00 , B01J19/0046 , B01J2219/00313 , B01J2219/00315 , B01J2219/00527 , B01J2219/00529 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00621 , B01J2219/00626 , B01J2219/00637 , B01J2219/00644 , B01J2219/00659 , B01J2219/00662 , B01J2219/00675 , B01J2219/00722 , C12N15/10 , C12Q1/6806 , C12Q1/6811 , C12Q1/6834 , C12Q1/6837 , C12Q1/6853 , C12Q1/686 , C12Q1/6874 , C40B40/06 , C40B50/14 , C40B60/14 , Y10S435/81 , Y10S436/808 , C12Q2563/179 , C12Q2531/107 , C12Q2525/131 , C12Q2525/191 , C12Q2565/519 , C12Q2565/537 , C12Q2525/179
摘要： A method of sorting mixtures of nucleic acid strands comprising hybridizing the strands to an array of immobilized oligonucleotides, each of which includes a constant segment adjacent to a variable segment. The constant segment of the immobilized oligonucleotides can be made complementary to the ends of strands obtained by digesting a double-stranded nucleic acid with a restriction enzyme and restoring the restriction sites, thereby permitting the sorting of strands according to their variable sequences adjacent to their constant terminal restored restriction sites.
摘要翻译：一种分选核酸链的混合物的方法，其包括将所述链杂交至固定化寡核苷酸阵列，其中每一个包括与可变区相邻的恒定区。可以使固定的寡核苷酸的恒定区域与通过用限制酶消化双链核酸获得的链的末端互补，并恢复限制性位点，从而允许根据它们与其常数相邻的可变序列对链进行分选终端恢复限制站点。

2. 发明申请

US20100197509A1 Novel oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids 审中-公开
标题翻译：新型寡核苷酸阵列及其用于分选，分离，测序和操作核酸的用途
公开(公告)号：US20100197509A1
公开(公告)日：2010-08-05
申请号：US12498267
申请日：2009-07-06
申请人： Alexander B. Chetverin , Fred Russell Kramer
发明人： Alexander B. Chetverin , Fred Russell Kramer
IPC分类号： C12Q1/68 , C40B30/00 , C40B50/18
CPC分类号： B82Y30/00 , B01J19/0046 , B01J2219/00313 , B01J2219/00315 , B01J2219/00527 , B01J2219/00529 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00621 , B01J2219/00626 , B01J2219/00637 , B01J2219/00644 , B01J2219/00659 , B01J2219/00662 , B01J2219/00675 , B01J2219/00722 , C12N15/10 , C12Q1/6806 , C12Q1/6811 , C12Q1/6834 , C12Q1/6837 , C12Q1/6853 , C12Q1/686 , C12Q1/6874 , C40B40/06 , C40B50/14 , C40B60/14 , Y10S435/81 , Y10S436/808 , C12Q2563/179 , C12Q2531/107 , C12Q2525/131 , C12Q2525/191 , C12Q2565/519 , C12Q2565/537 , C12Q2525/179
摘要： A method of sorting mixtures of nucleic acid strands comprising hybridizing the strands to an array of immobilized oligonucleotides, each of which includes a constant segment adjacent to a variable segment. The constant segment of the immobilized oligonucleotides can be made complementary to the ends of strands obtained by digesting a double-stranded nucleic acid with a restriction enzyme and restoring the restriction sites, thereby permitting the sorting of strands according to their variable sequences adjacent to their constant terminal restored restriction sites.
摘要翻译：一种分选核酸链的混合物的方法，其包括将所述链杂交至固定化寡核苷酸阵列，其中每一个包括与可变区相邻的恒定区。可以使固定的寡核苷酸的恒定区域与通过用限制酶消化双链核酸获得的链的末端互补，并恢复限制性位点，从而允许根据它们与其常数相邻的可变序列对链进行分选终端恢复限制站点。

3. 发明授权

US6103463A Method of sorting a mixture of nucleic acid strands on a binary array 失效
标题翻译：在二进制阵列上排列核酸链的混合物的方法
公开(公告)号：US6103463A
公开(公告)日：2000-08-15
申请号：US247530
申请日：1994-05-23
申请人： Alexander B. Chetverin , Fred Russell Kramer
发明人： Alexander B. Chetverin , Fred Russell Kramer
IPC分类号： B01J19/00 , C12M1/34 , C12N15/10 , C12Q1/68 , C40B40/06 , C40B50/14 , C40B60/14
CPC分类号： B82Y30/00 , B01J19/0046 , C12N15/10 , C12Q1/6806 , C12Q1/6811 , C12Q1/6834 , C12Q1/6837 , C12Q1/6853 , C12Q1/686 , C12Q1/6874 , B01J2219/00313 , B01J2219/00315 , B01J2219/00527 , B01J2219/00529 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00621 , B01J2219/00626 , B01J2219/00637 , B01J2219/00644 , B01J2219/00659 , B01J2219/00662 , B01J2219/00675 , B01J2219/00722 , C40B40/06 , C40B50/14 , C40B60/14 , Y10S435/81 , Y10S436/808
摘要： A method of sorting mixtures of nucleic acid strands comprising hybridizing the strands to an array of immobilized oligonucleotides, each of which includes a constant segment adjacent to a variable segment. The constant segment of the immobilized oligonucleotides can be made complementary to the ends of strands obtained by digesting a double-stranded nucleic acid with a restriction enzyme and restoring the restriction sites, thereby permitting the sorting of strands according to their variable sequences adjacent to their constant terminal restored restriction sites.
摘要翻译：一种分选核酸链的混合物的方法，其包括将所述链杂交至固定化寡核苷酸阵列，其中每一个包括与可变区相邻的恒定区。可以使固定的寡核苷酸的恒定区域与通过用限制酶消化双链核酸获得的链的末端互补，并恢复限制性位点，从而允许根据它们与其常数相邻的可变序列对链进行分选终端恢复限制站点。

4. 发明授权

US06322971B1 Oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids 失效
标题翻译：寡核苷酸阵列及其用于分选，分离，测序和操作核酸的用途
公开(公告)号：US06322971B1
公开(公告)日：2001-11-27
申请号：US09164249
申请日：1998-09-30
申请人： Alexander B. C{acute over (h)}etverin , Fred Russell Kramer
发明人： Alexander B. C{acute over (h)}etverin , Fred Russell Kramer
IPC分类号： C12Q168
CPC分类号： B82Y30/00 , B01J19/0046 , B01J2219/00313 , B01J2219/00315 , B01J2219/00527 , B01J2219/00529 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00621 , B01J2219/00626 , B01J2219/00637 , B01J2219/00644 , B01J2219/00659 , B01J2219/00662 , B01J2219/00675 , B01J2219/00722 , C12N15/10 , C12Q1/6806 , C12Q1/6811 , C12Q1/6834 , C12Q1/6837 , C12Q1/6853 , C12Q1/686 , C12Q1/6874 , C40B40/06 , C40B50/14 , C40B60/14 , Y10S435/81 , Y10S436/808 , C12Q2563/179 , C12Q2531/107 , C12Q2525/131 , C12Q2525/191 , C12Q2565/519 , C12Q2565/537 , C12Q2525/179
摘要： Ligation methods for manipulating nucleic acid stands and oligonucleotides utilizing hybridization arrays of immobilized oligonucleotides. The oligonucleotide arrays may be plain or sectioned, comprehensive or non-comprehensive. The immobilized oligonucleotides may in some cases be binary oligonucleotides having constant as well as variable segments. Some embodiments include amplification of ligated products.
摘要翻译：使用固定化寡核苷酸杂交阵列来操纵核酸位点和寡核苷酸的连接方法。寡核苷酸阵列可以是平坦的或分段的，全面的或不全面的。在一些情况下，固定的寡核苷酸可以是具有恒定以及可变区的二元寡核苷酸。一些实施方案包括连接产物的扩增。

5. 发明授权

US6150097A Nucleic acid detection probes having non-FRET fluorescence quenching and kits and assays including such probes 失效
标题翻译：具有非FRET荧光猝灭的核酸检测探针和包括这种探针的试剂盒和测定
公开(公告)号：US6150097A
公开(公告)日：2000-11-21
申请号：US990176
申请日：1997-12-12
申请人： Sanjay Tyagi , Fred Russell Kramer
发明人： Sanjay Tyagi , Fred Russell Kramer
IPC分类号： C12N15/09 , C07H21/04 , C12Q1/68
CPC分类号： C12Q1/6818 , C12Q1/6816 , Y10S435/81 , Y10S436/80
摘要： Nucleic acid hybridization probes having a first conformation when not interacting with a target and a second conformation when interacting with a target, and having the ability to bring a label pair into touching contact in one conformation but not the other, are labeled with a non-FRET pair of chromophores and generate a fluorescent or absorbance signal. Kits may include such probes, and assays, including multiplex assays, may utilize such probes.
摘要翻译：当与靶相互作用时具有第一构象的核酸杂交探针和当与靶相互作用时具有第二构象，并且具有使标记对在一个构象而不是另一个构型中的接触接触的能力用非 - FRET对发色团并产生荧光或吸光度信号。试剂盒可以包括这样的探针，并且包括多重测定在内的测定可以利用这样的探针。

6. 发明授权

US06420539B1 Replicatable hybridizable recombinant RNA probes and methods of using same 失效
标题翻译：可复制的可混合重组RNA探针及其使用方法
公开(公告)号：US06420539B1
公开(公告)日：2002-07-16
申请号：US08484992
申请日：1995-06-07
申请人： Fred Russell Kramer , Paul M. Lizardi , Eleanor Ann Miele , Donald R. Mills
发明人： Fred Russell Kramer , Paul M. Lizardi , Eleanor Ann Miele , Donald R. Mills
IPC分类号： C07H2104
CPC分类号： G01N33/532 , C07H21/00 , C12N15/10 , C12P19/34 , C12Q1/6813 , C12Q1/682 , C12Q1/6832 , C12Q1/6867 , C12Q1/6876 , C12Q1/6893 , C12Q1/703 , Y10S435/948
摘要： The present invention provides a replicatable and hybridizable recombinant single-stranded RNA probe molecule comprising: a recognition sequence for the binding of an RNA-directed RNA polymerase; a sequence required for the initiation of product strand synthesis by the polymerase; and a heteroloqus RNA sequence inserted at a specific site in the internal region of the recombinant molecule and complementary to an oligo or polynucleotide of interest. This invention also provides methods for determining the presence of concentration of an oligo- or polynucleotide of interest in a sample and for simultaneously determining the presence or concentration of several different oligo- or polynucleotides of interest in a sample.
摘要翻译：本发明提供可重复和可杂交的重组单链RNA探针分子，其包含：用于结合RNA指导的RNA聚合酶的识别序列; 通过聚合酶引发产物链合成所需的序列; 和杂交RNA序列插入重组分子的内部区域的特定部位，并与感兴趣的寡核苷酸或多核苷酸互补。本发明还提供了用于确定样品中目的寡聚物或多核苷酸的浓度的存在以及用于同时测定样品中几种不同的目标的寡核苷酸或多核苷酸的存在或浓度的方法。

7. 发明授权

US5871976A Autocatalytic replication of recombinant RNA 失效
标题翻译：重组RNA的自动催化复制
公开(公告)号：US5871976A
公开(公告)日：1999-02-16
申请号：US467816
申请日：1995-06-06
申请人： Fred Russell Kramer , Eleanor Anne Miele , Donald Robert Mills
发明人： Fred Russell Kramer , Eleanor Anne Miele , Donald Robert Mills
IPC分类号： C12N15/10 , C12P19/34 , C12Q1/68
CPC分类号： C12N7/00 , C12N15/10 , C12P19/34 , C12Q1/6867 , C12N2795/10122 , C12N2795/18122
摘要： This invention provides a method for producing a mutant virus or viroid, or a mutant recombinant RNA molecule comprising a single stranded virus genome or a single stranded viroid genome, a circular virus genome or a circular viroid genome, or a segment of a virus genome or a segment of a viroid genome which comprises incubating a recombinant single-stranded RNA molecule comprising a recognition sequence for the binding of an RNA directed RNA polymerase, a sequence for the initiation of product strand synthesis by the polymerase and a heterologous sequence of interest derived from a different RNA molecule inserted at a specific site in the internal region of the recombinant molecule under appropriate selective conditions and for a sufficient period of time permitting the selection of a mutant population, the heterologous sequence of interest comprising the RNA of the single stranded virus genome or the single stranded viroid genome, the circular virus genome or the circular viroid genome or a segment of the virus genome or a segment of the viroid genome.
摘要翻译：本发明提供了用于产生突变病毒或病毒的方法或包含单链病毒基因组或单链病毒基因组，环状病毒基因组或环状病毒基因组或病毒基因组的片段的突变型重组RNA分子或一种病毒基因组的片段，其包括孵育包含用于RNA定向RNA聚合酶结合的识别序列的重组单链RNA分子，通过聚合酶引发产物链合成的序列和衍生自在合适的选择性条件下插入重组分子的内部区域的特定位点的不同RNA分子，并且允许选择突变群体足够长的时间段，包含单链病毒基因组的RNA的异源感兴趣序列或单链类病毒基因组，环状病毒基因组或环状病毒基因组我或病毒基因组的一段或病毒基因组的片段。

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