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1. 发明申请

US20080131891A1 Biochemical Reagents And Their Uses 审中-公开
标题翻译：生化试剂及其用途
公开(公告)号：US20080131891A1
公开(公告)日：2008-06-05
申请号：US11814849
申请日：2006-02-01
申请人： David James Squirrell , Martin Alan Lee
发明人： David James Squirrell , Martin Alan Lee
IPC分类号： C12Q1/68 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/6818 , C12Q1/6823 , C12Q1/6848 , C12Q2525/301 , C12Q2521/337
摘要： A method for adding a first and a second functional nucleic acid sequence to a reaction mixture, in particular an amplification reaction mixture in a predetermined stoichiometry and/or at a predetermined point in time, said method comprising adding to the reaction mixture an oligonucleotide comprising a first and a second functional nucleic acid sequence separated by a spacer sequence, said spacer sequence comprising a region which, when double stranded, comprises a cleavable region, forming a cleavable double stranded region within the spacer region of said oligonucleotide, and cleaving the double stranded region within said oligonucleotide. Oligonucleotides for use in the method, and comprising a first and a second functional nucleic acid sequence, such as primers or probes used in an amplification reaction, separated by a spacer sequence, is also provided.
摘要翻译：一种将第一和第二功能性核酸序列以预定的化学计量和/或在预定时间点向反应混合物，特别是扩增反应混合物中加入的方法，所述方法包括向反应混合物中加入包含第一和第二功能性核酸序列由间隔序列分隔，所述间隔序列包含当双链时包含可切割区的区域，在所述寡核苷酸的间隔区内形成可切割双链区，并切割双链所述寡核苷酸内的区域。还提供了用于该方法并包含第一和第二功能性核酸序列的寡核苷酸，例如扩增反应中使用的引物或探针，由间隔序列分隔。

2. 发明申请

US20080166700A1 Method For Detecting Viable Cells In A Sample By Using A Virus 有权
标题翻译：通过使用病毒检测样品中活细胞的方法
公开(公告)号：US20080166700A1
公开(公告)日：2008-07-10
申请号：US11816331
申请日：2006-02-16
申请人： David James Squirrell , Martin Alan Lee
发明人： David James Squirrell , Martin Alan Lee
IPC分类号： C12Q1/70 , C12N7/00
CPC分类号： C12Q1/689 , C12Q1/70
摘要： A method for detecting viable cells such as bacterial cells, within a sample, said method comprising (i) incubating said sample with a virus which is able to infect said cells under conditions which allow said virus to infect and replicate within any such cells which are viable; (ii) detecting any nucleic acid obtained by replication of the virus in said cell.
摘要翻译：一种用于检测样品内的活细胞如细菌细胞的方法，所述方法包括（i）在允许所述病毒在任何这样的细胞内感染和复制的条件下，使能够感染所述细胞的病毒孵育所述样品，所述病毒是可行的（ii）检测通过在所述细胞中复制病毒获得的任何核酸。

3. 发明申请

US20080206751A1 Method For Carrying Out A Multi-Step Reaction, Breakable Container For Storing Reagents And Method For Transferring Solid Reagent Using An Electrostatically Charged Wand 审中-公开
标题翻译：用于进行多步反应的方法，用于储存试剂的易碎容器和使用静电充电棒转移固体试剂的方法
公开(公告)号：US20080206751A1
公开(公告)日：2008-08-28
申请号：US11814726
申请日：2006-01-26
申请人： David James Squirrell , Martin Alan Lee
发明人： David James Squirrell , Martin Alan Lee
IPC分类号： C12Q1/68 , B01L3/00 , C12P19/34 , B65G61/00 , B65H29/00
CPC分类号： B01J19/0046 , B01J2219/00283 , B01J2219/00335 , B01J2219/00344 , B01J2219/00421 , B01J2219/00468 , B01J2219/00704 , B01J2219/00722 , B01L3/502 , B01L3/5021 , B01L7/52 , B01L2200/16 , B01L2300/0838 , B01L2300/087 , B01L2400/0409 , C40B40/06 , G01N35/0098
摘要： The application relates to a method of performing a multi-step reaction vessel (68) having at least two compartments (685, 680). The reagents are placed in the first compartment (685) and moved to second one (680) by centrifugation, after which another set of reagents may be placed in the first compartment (685) while the reaction in the lower chamber takes place. Once the reaction is complete, the reagents that were in the first compartment (685) may be moved to the lower one (680) by centrifugation. The application also claims a container having a pierceable lower surface and an upper surface with either a pierceable component or a lid. A wand capable of being electrostatically charged, an apparatus comprising such a wand and a method of transferring solid reagents using such a wand is also claimed.
摘要翻译：本申请涉及执行具有至少两个隔室（685,680）的多步骤反应容器（68）的方法。将试剂置于第一隔室（685）中，并通过离心移至第二隔室（680），之后另外一组试剂可放置在第一隔室（685）中，同时在下室中发生反应。一旦反应完成，通过离心可将第一隔室（685）中的试剂移至较低的试剂（680）。本申请还要求具有可刺穿的下表面的容器和具有可刺破的部件或盖子的上表面。一种能够被静电充电的魔杖，包括这种魔杖的装置以及使用这种魔杖传送固体试剂的方法也被要求保护。

4. 发明申请

US20080193936A1 Polymorphism Detection Method 审中-公开
标题翻译：多态性检测方法
公开(公告)号：US20080193936A1
公开(公告)日：2008-08-14
申请号：US11909218
申请日：2006-03-22
申请人： David James Squirrell
发明人： David James Squirrell
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q2561/125 , C12Q2521/501 , C12Q2521/101
摘要： A method for detecting the presence or absence of a polymorphism at a particular position within a nucleic acid sequence in a sample, said method comprising i) annealing a pair of probes to said nucleic acid sequence, said probes being designed such that they anneal adjacent each other, and only completely anneal to one form of the sequence, in a reaction mixture which is substantially free of ATP; ii) ligating together any completely annealed pairs of probes in the presence of NAD and a nucleic acid ligase which uses NAD as a substrate/ iii) phosphorylating any AMP released by a ligation event to ATP, and iv) detecting ATP, in particular using a bioluminescent reaction, in the resultant reaction mixture. Kits for conducting this method are also described and claimed.
摘要翻译：一种用于检测样品中核酸序列内特定位置上存在或不存在多态性的方法，所述方法包括：i）将一对探针退火至所述核酸序列，所述探针被设计为使得它们与每个在基本上不含ATP的反应混合物中，仅对序列的一种形式进行完全退火; ii）在存在NAD和使用NAD作为底物的核酸连接酶的情况下将任何完全退火的探针对连接在一起; iii）将通过连接事件释放的任何AMP磷酸化成ATP，以及iv）检测ATP，特别是使用在所得反应混合物中进行生物发光反应。还描述和要求保护用于进行该方法的套件。

5. 发明申请

US20120141976A1 METHOD FOR DETECTING VIABLE CELLS IN A SAMPLE BY USING A VIRUS 有权
标题翻译：通过使用病毒检测样品中的可见细胞的方法
公开(公告)号：US20120141976A1
公开(公告)日：2012-06-07
申请号：US13276772
申请日：2011-10-19
申请人： David James Squirrell , Martin Alan Lee
发明人： David James Squirrell , Martin Alan Lee
IPC分类号： C12Q1/70
CPC分类号： C12Q1/689 , C12Q1/70
摘要： A method for detecting viable cells such as bacterial cells, within a sample, said method comprising (i) incubating said sample with a virus which is able to infect said cells under conditions which allow said virus to infect and replicate within any such cells which are viable; and (ii) detecting any nucleic acid obtained by replication of the virus in said cell.
摘要翻译：一种用于检测样品内的活细胞如细菌细胞的方法，所述方法包括（i）在允许所述病毒在任何这样的细胞内感染和复制的条件下，使能够感染所述细胞的病毒孵育所述样品，所述病毒是可行的和（ii）检测通过在所述细胞中复制病毒获得的任何核酸。

6. 发明授权

US08071337B2 Method for detecting viable cells in a sample by using a virus 有权
标题翻译：通过使用病毒检测样品中的活细胞的方法
公开(公告)号：US08071337B2
公开(公告)日：2011-12-06
申请号：US11816331
申请日：2006-02-16
申请人： David James Squirrell , Martin Alan Lee
发明人： David James Squirrell , Martin Alan Lee
IPC分类号： C12P19/34
CPC分类号： C12Q1/689 , C12Q1/70
摘要： A method for detecting viable cells such as bacterial cells, within a sample, said method comprising (i) incubating said sample with a virus which is able to infect said cells under conditions which allow said virus to infect and replicate within any such cells which are viable; (ii) detecting any nucleic acid obtained by replication of the virus in said cell.
摘要翻译：一种用于检测样品内的活细胞如细菌细胞的方法，所述方法包括（i）在允许所述病毒在任何这样的细胞内感染和复制的条件下，使能够感染所述细胞的病毒孵育所述样品，所述病毒是可行的（ii）检测通过在所述细胞中复制病毒获得的任何核酸。

7. 发明申请

US20110244454A1 FREEZE-DRIED COMPOSITIONS FOR BIOCHEMICAL REACTIONS 审中-公开
标题翻译：冷冻干燥的组合物用于生物化学反应
公开(公告)号：US20110244454A1
公开(公告)日：2011-10-06
申请号：US13001208
申请日：2009-07-01
申请人： Felix Franks , Samantha Spiers , Martin Lee , Diane Sutton
发明人： Felix Franks , Samantha Spiers , Martin Lee , Diane Sutton
IPC分类号： C12Q1/68 , C12N9/12 , C12P19/34
CPC分类号： C07H21/00 , C12Q1/6806 , C12Q2527/125
摘要： The use of raffinose as a glass-forming agent for freeze-dried compositions intended for use in conducting chemical or biochemical reactions such as the PCR. Thus, for instance, there is provided a composition for carrying out a chemical or biochemical reaction, said composition being in a freeze-dried form and comprising (i) a set of reagents comprising at least some of the chemical or biochemical reagents necessary for conducting said chemical or biochemical reaction, and (ii) raffinose. Kits comprising these compositions and methods of using them form a further aspect of the invention.
摘要翻译：使用棉子糖作为用于进行化学或生物化学反应如PCR的冷冻干燥组合物的玻璃形成剂。因此，例如，提供了用于进行化学或生物化学反应的组合物，所述组合物为冷冻干燥形式，并且包括（i）一组试剂，其包含至少一些进行所需的化学或生化试剂表示化学或生化反应，和（ii）棉子糖。包含这些组合物的组合物和使用它们的方法形成本发明的另一方面。

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