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1. 发明申请

US20080216198A1 Doubled haploid cells, embryos and plants 审中-公开
标题翻译：双倍体单倍体细胞，胚胎和植物
公开(公告)号：US20080216198A1
公开(公告)日：2008-09-04
申请号：US12054556
申请日：2008-03-25
申请人： Zuo-Yu Zhao , Dennis L. Bidney , Evan D. Elsing , Michael D. Miller , Xinli E. Wu , William J. Gordon-Kamm
发明人： Zuo-Yu Zhao , Dennis L. Bidney , Evan D. Elsing , Michael D. Miller , Xinli E. Wu , William J. Gordon-Kamm
IPC分类号： C12N15/29 , C12N15/11
CPC分类号： C12N15/8241 , A01H5/10 , C12N15/8201 , C12N15/829
摘要： Methods for producing homozygous plants, seeds, and plant cells are provided. Also provided are methods of transformation.
摘要翻译：提供了用于产生纯合植物，种子和植物细胞的方法。还提供了转化的方法。

2. 发明授权

US08859846B2 Doubling of chromosomes in haploid embryos 有权
标题翻译：染色体倍增在单倍体胚胎
公开(公告)号：US08859846B2
公开(公告)日：2014-10-14
申请号：US11532921
申请日：2006-09-19
申请人： Joanne E. Barton , Sheila E. Maddock , Xinli E. Wu , Zuo-Yu Zhao , Mark E. Williams , Tanveer Hussain , William J. Gordon-Kamm
发明人： Joanne E. Barton , Sheila E. Maddock , Xinli E. Wu , Zuo-Yu Zhao , Mark E. Williams , Tanveer Hussain , William J. Gordon-Kamm
IPC分类号： A01H1/08 , A01H4/00
CPC分类号： A01H1/08
摘要： Methods for producing doubled haploid corn (Zea mays) plants, seeds, and plant cells are provided.
摘要翻译：提供了生产双倍单倍体玉米（Zea mays）植物，种子和植物细胞的方法。

3. 发明申请

US20080216191A1 Doubling of Chromosomes in Haploid Embryos 有权
标题翻译：染色体倍倍数在单倍体胚胎
公开(公告)号：US20080216191A1
公开(公告)日：2008-09-04
申请号：US11532921
申请日：2006-09-19
申请人： Joanne E. Barton , Sheila E. Maddock , Xinli E. Wu , Zuo-Yu Zhao , Mark E. Williams , Tanveer Hussain , William J. Gordon-Kamm
发明人： Joanne E. Barton , Sheila E. Maddock , Xinli E. Wu , Zuo-Yu Zhao , Mark E. Williams , Tanveer Hussain , William J. Gordon-Kamm
IPC分类号： A01H1/08 , A01H5/00
CPC分类号： A01H1/08
摘要： Methods for producing doubled haploid corn (Zea mays) plants, seeds, and plant cells are provided.
摘要翻译：提供了生产双倍单倍体玉米（Zea mays）植物，种子和植物细胞的方法。

4. 发明申请

US20070143880A1 Methods for Introducing Into a Plant a Polynucleotide of Interest 审中-公开
标题翻译：向植物引入感兴趣的多核苷酸的方法
公开(公告)号：US20070143880A1
公开(公告)日：2007-06-21
申请号：US11427371
申请日：2006-06-29
申请人： Dennis L. Bidney , Sheila E. Maddock , Xinli E. Wu , Zuo-Yu Zhao
发明人： Dennis L. Bidney , Sheila E. Maddock , Xinli E. Wu , Zuo-Yu Zhao
IPC分类号： A01H1/00 , C12N15/82
CPC分类号： C12N15/8205 , C12N15/8207 , C12N15/8213
摘要： The present invention provides methods and compositions which deliver Agrobacterium via microinjection directly into the embryo sac. At the time of injection, the embryo sac can comprise an egg cell, or alternatively, the embryo sac can be fertilized and comprise either a zygote or an embryo. Once inside the embryo sac, the Agrobacterium harboring a T-DNA having a polynucleotide of interest can express of the polynucleotide of interest in the plant. Further, the Agrobacterium can transfer the T-DNA having the polynucleotide of interest to the plant nucleus to produce a transformed plant. The polynucleotide of interest may be stably integrated into the genome of the egg cell, zygote, embryo, or endosperm, and any tissue, plant part, and/or plant generated therefrom.
摘要翻译：本发明提供了通过显微注射将土壤杆菌直接递送到胚囊中的方法和组合物。在注射时，胚囊可以包括卵细胞，或者，胚囊可被受精并且包括受精卵或胚胎。一旦进入胚囊，携带具有感兴趣的多核苷酸的T-DNA的农杆菌可以在植物中表达感兴趣的多核苷酸。此外，农杆菌可以将具有目标多核苷酸的T-DNA转移到植物核，以产生转化的植物。感兴趣的多核苷酸可以稳定地整合到卵细胞，合子，胚或胚乳的基因组中，以及由其产生的任何组织，植物部分和/或植物。

5. 发明申请

US20110167516A1 METHODS AND COMPOSITIONS FOR THE INTRODUCTION AND REGULATED EXPRESSION OF GENES IN PLANTS 审中-公开
标题翻译：用于植物基因引入和调控表达的方法和组合物
公开(公告)号：US20110167516A1
公开(公告)日：2011-07-07
申请号：US12982180
申请日：2010-12-30
申请人： William J. Gordon-Kamm , Theodore M. Klein , Keith S. Lowe , Kevin E. McBride , Christopher J. Scelonge , Bing-Bing Wang , Ning Wang , Xinli E. Wu
发明人： William J. Gordon-Kamm , Theodore M. Klein , Keith S. Lowe , Kevin E. McBride , Christopher J. Scelonge , Bing-Bing Wang , Ning Wang , Xinli E. Wu
IPC分类号： A01H5/00 , C07H21/04 , C12N15/63 , C12N5/10 , A01H5/10 , C12N15/82
CPC分类号： C12N15/8241 , C12N15/8213 , C12N15/8216 , C12N15/8262
摘要： Compositions and methods are provided for the introduction and the regulated expression of genes in plants. Compositions include promoter constructs that provide a level of activity useful for the regulated expression of site-specific recombinases, while avoiding premature excision. Further provided are isolated polynucleotides encoding novel babyboom polypeptides, expression cassettes, and plants comprising the same. Methods for the introduction of genes into plants are provided, including methods for plastid transformation and methods for the transformation of tissues from mature seeds and leaves.
摘要翻译：为植物中基因的引入和调控表达提供了组合物和方法。组合物包括提供可用于调节表达位点特异性重组酶的活性水平的启动子构建体，同时避免过早切除。进一步提供了编码新型婴儿多肽，表达盒和包含其的植物的分离的多核苷酸。提供了将基因导入植物的方法，包括质体转化的方法和从成熟种子和叶子转化组织的方法。

6. 发明申请

US20070143881A1 Methods and Compositions for Improving the Efficiency of Site-Specific Polynucleotide Exchange 审中-公开
标题翻译：提高位点特异性多核苷酸交换效率的方法和组合
公开(公告)号：US20070143881A1
公开(公告)日：2007-06-21
申请号：US11427947
申请日：2006-06-30
申请人： Dennis L. Bidney , Sheila E. Maddock , Xinli E. Wu
发明人： Dennis L. Bidney , Sheila E. Maddock , Xinli E. Wu
IPC分类号： A01H1/00
CPC分类号： C12N15/8213 , C12N15/8205 , C12N15/8207
摘要： Methods and compositions using a site-specific integration system are combined with methods and compositions which deliver compositions via microinjection directly to the embryo sac of a plant. The methods allow for various components of the site-specific recombination system to be introduced into the cellular environment of the embryo sac a composition comprising at least one component of the site-specific recombination system is injected into an embryo sac, providing improved efficiency of expression, recombination, integration, exchange, excision and/or inversion of a polynucleotide of interest. The polynucleotide of interest may be stably integrated into the genome of the egg cell, zygote, embryo, or endosperm, and tissues, plant parts, and/or plants produced therefrom. Cells, egg cells, zygotes, embryos, endosperm, tissues, seeds, and/or plants produced by the methods and comprising the polynucleotide(s) of interest are also provided.
摘要翻译：使用位点特异性整合系统的方法和组合物与通过显微注射将组合物直接递送至植物胚囊的方法和组合物组合。所述方法允许将位点特异性重组系统的各种组分引入胚囊的细胞环境中，将包含位点特异性重组系统的至少一个组分的组合物注入胚囊，提供更好的表达效率，重组，整合，交换，切除和/或转化感兴趣的多核苷酸。感兴趣的多核苷酸可以稳定地整合到卵细胞，合子，胚或胚乳以及由其产生的组织，植物部分和/或植物的基因组中。还提供了通过方法产生并包含感兴趣的多核苷酸的细胞，卵细胞，合子，胚胎，胚乳，组织，种子和/或植物。

7. 发明授权

US08404930B2 Methods for improving monocot transformation 失效
标题翻译：改善单子叶植物转化的方法
公开(公告)号：US08404930B2
公开(公告)日：2013-03-26
申请号：US12694013
申请日：2010-01-26
申请人： Xinli E. Wu , Myeong-Je Cho , Zuo-Yu Zhao
发明人： Xinli E. Wu , Myeong-Je Cho , Zuo-Yu Zhao
IPC分类号： C12N15/84 , A01H4/00
CPC分类号： C12N15/8205
摘要： The invention provides improved plant transformation methods. In particular the method provides increased transformation frequency, especially in recalcitrant plants. The method includes various transformation protocols for monocots, such as maize and sorghum, using a combination of media and light conditions to achieve increased efficiency of monocot transformation and increased callus initiation frequencies.
摘要翻译：本发明提供改进的植物转化方法。特别地，该方法提供增加的转化频率，特别是在顽固植物中。该方法包括用于单子叶植物（例如玉米和高粱）的各种转化方案，其使用培养基和光照条件的组合来实现提高单子叶植物转化的效率和增加的愈伤组织起始频率。

8. 发明授权

US08389807B2 Method for increasing efficiency of germplasm screening in plant transformation 失效
标题翻译：提高植物转化种质筛选效率的方法
公开(公告)号：US08389807B2
公开(公告)日：2013-03-05
申请号：US12649673
申请日：2009-12-30
申请人： Zuo-Yu Zhao , Xinli E. Wu , Myoeng-Je Cho , Deping Xu
发明人： Zuo-Yu Zhao , Xinli E. Wu , Myoeng-Je Cho , Deping Xu
IPC分类号： C12N15/84 , A01H4/00 , A01H1/04
CPC分类号： C12N15/8209 , A01H1/04 , C12N15/8201 , C12N15/8205
摘要： A method for increasing efficiency of germplasm screening for transformability may include providing a plurality of lines of plant target tissue to be transformed, characterizing each of the lines to provide characterization data, the characterization data comprises DNA or nucleic acid delivery technique response data and tissue culture response data, eliminating one or more of the plurality of lines based on the characterization data without performing transformation of the plurality of lines, such that a subset of the plurality of lines remains, and performing transformation experiments on the subset of the plurality of lines. The method may also include selecting a DNA or nucleic acid delivery technique protocol and a tissue culture protocol prior to the characterization.
摘要翻译：提高种质筛选效率的方法可包括提供要转化的多条植物靶组织，表征各条线以提供特征数据，表征数据包括DNA或核酸递送技术应答数据和组织培养响应数据，基于所述表征数据消除所述多行中的一个或多个，而不执行所述多条线的变换，使得保留所述多条线的子集，并对所述多条线的所述子集执行变换实验。该方法还可以包括在表征之前选择DNA或核酸递送技术方案和组织培养方案。

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