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1. 发明授权

US08623324B2 Luminescent dyes with a water-soluble intramolecular bridge and their biological conjugates 有权
标题翻译：具有水溶性分子内桥的发光染料及其生物偶联物
公开(公告)号：US08623324B2
公开(公告)日：2014-01-07
申请号：US13181107
申请日：2011-07-12
申请人： Timothy Dubrovsky , Barnaby Abrams , Zhenjun Diwu , Qinglin Meng , Jinfang Liao , Haitao Guo
发明人： Timothy Dubrovsky , Barnaby Abrams , Zhenjun Diwu , Qinglin Meng , Jinfang Liao , Haitao Guo
IPC分类号： A61K51/00 , A61M36/14
CPC分类号： C09B23/083 , C09B11/08 , C09B11/24 , C09B11/26 , C09B57/00 , C09B57/10 , G01N33/533
摘要： Chemically reactive dyes that are intramolecularly crosslinked with a water-soluble bridge, their bioconjugates and their uses are described. Reactive fluorescent dyes that have a water-soluble bridge are superior to those of conjugates of spectrally non-crosslinked dyes or the dyes that are crosslinked with a hydrophobic bridge. The invention includes reactive fluorescent dyes, their biological conjugates and uses.
摘要翻译：描述了与水溶性桥分子内交联的化学活性染料及其生物共轭物及其用途。具有水溶性桥梁的活性荧光染料优于光谱非交联染料或与疏水桥交联的染料的共轭物。本发明包括反应性荧光染料，它们的生物共轭物和用途。

2. 发明申请

US20100255504A1 Reactive Heterocycle-Substituted 7-Hydroxycoumarins and Their Conjugates 有权
标题翻译：反应性杂环取代的7-羟基香豆素及其偶联物
公开(公告)号：US20100255504A1
公开(公告)日：2010-10-07
申请号：US12726267
申请日：2010-03-17
申请人： Zhenjun Diwu , Timothy Dubrovsky , Barnaby Abrams , Jinfang Liao , Qinglin Meng
发明人： Zhenjun Diwu , Timothy Dubrovsky , Barnaby Abrams , Jinfang Liao , Qinglin Meng
IPC分类号： G01N33/53 , C07K16/00 , C07D405/10 , C07D417/04 , C07D405/04 , C07D401/12
CPC分类号： C07D405/04 , C07D405/14 , C07D409/04 , C07D409/14 , C07D417/04 , C07D417/14 , C09B57/02 , Y10T436/13
摘要： Chemically-reactive, water-soluble, heterocycle-substituted 7-hydroxycoumarin dyes, their bioconjugates and uses are described. The conjugates derived from reactive heterocycle-substituted 7-hydroxycoumarin dyes are used for analyzing biological compounds. These heterocycle-substituted 7-hydroxycoumarin dyes are particularly useful as fluorescent labels for biopolymer detection reagents, such as antibodies or nucleic acid probes. The dye-antibody conjugates of the invention are particularly useful for analyzing analytes using a flow cytometer equipped with a violet laser as an excitation source due to their strong absorption at 405 nm and high fluorescence quantum yield.
摘要翻译：描述了化学反应性，水溶性，杂环取代的7-羟基香豆素染料，它们的生物共轭物和用途。衍生自反应性杂环取代的7-羟基香豆素染料的共轭物用于分析生物化合物。这些杂环取代的7-羟基香豆素染料特别可用作生物聚合物检测试剂如抗体或核酸探针的荧光标记。本发明的染料 - 抗体缀合物由于其在405nm下的强吸收和高荧光量子产率而特别适用于使用配有紫激光作为激发源的流式细胞仪分析分析物。

3. 发明授权

US08431416B2 Reactive heterocycle-substituted 7-hydroxycoumarins and their conjugates 有权
标题翻译：活性杂环取代的7-羟基香豆素及其偶联物
公开(公告)号：US08431416B2
公开(公告)日：2013-04-30
申请号：US12726267
申请日：2010-03-17
申请人： Zhenjun Diwu , Timothy Dubrovsky , Barnaby Abrams , Jinfang Liao , Qinglin Meng
发明人： Zhenjun Diwu , Timothy Dubrovsky , Barnaby Abrams , Jinfang Liao , Qinglin Meng
IPC分类号： G01N33/533 , C07K1/13 , C07K16/00
CPC分类号： C07D405/04 , C07D405/14 , C07D409/04 , C07D409/14 , C07D417/04 , C07D417/14 , C09B57/02 , Y10T436/13
摘要： Chemically-reactive, water-soluble, heterocycle-substituted 7-hydroxycoumarin dyes, their bioconjugates and uses are described. The conjugates derived from reactive heterocycle-substituted 7-hydroxycoumarin dyes are used for analyzing biological compounds. These heterocycle-substituted 7-hydroxycoumarin dyes are particularly useful as fluorescent labels for biopolymer detection reagents, such as antibodies or nucleic acid probes. The dye-antibody conjugates of the invention are particularly useful for analyzing analytes using a flow cytometer equipped with a violet laser as an excitation source due to their strong absorption at 405 nm and high fluorescence quantum yield.
摘要翻译：描述了化学反应性，水溶性，杂环取代的7-羟基香豆素染料，它们的生物共轭物和用途。衍生自反应性杂环取代的7-羟基香豆素染料的共轭物用于分析生物化合物。这些杂环取代的7-羟基香豆素染料特别可用作生物聚合物检测试剂如抗体或核酸探针的荧光标记。本发明的染料 - 抗体缀合物由于其在405nm下的强吸收和高荧光量子产率而特别适用于使用配有紫激光作为激发源的流式细胞仪分析分析物。

4. 发明申请

US20120183954A1 LUMINESCENT DYES WITH A WATER-SOLUBLE INTRAMOLECULAR BRIDGE AND THEIR BIOLOGICAL CONJUGATES 有权
标题翻译：具有水溶性分子桥的发光体及其生物学结合
公开(公告)号：US20120183954A1
公开(公告)日：2012-07-19
申请号：US13181107
申请日：2011-07-12
申请人： Zhenjun Diwu , Qinglin Meng , Jinfang Liao , Haitao Guo , Timothy Dubrovsky , Barnaby Abrams
发明人： Zhenjun Diwu , Qinglin Meng , Jinfang Liao , Haitao Guo , Timothy Dubrovsky , Barnaby Abrams
IPC分类号： G01N21/64 , C07D498/16 , C07F5/00 , C12N9/96 , C07D403/14 , C07K17/02 , C08B37/02 , C07H19/00 , C07K5/00 , C07D487/14
CPC分类号： C09B23/083 , C09B11/08 , C09B11/24 , C09B11/26 , C09B57/00 , C09B57/10 , G01N33/533
摘要： Chemically reactive dyes that are intramolecularly crosslinked with a water-soluble bridge, their bioconjugates and their uses are described. Reactive fluorescent dyes that have a water-soluble bridge are superior to those of conjugates of spectrally non-crosslinked dyes or the dyes that are crosslinked with a hydrophobic bridge. The invention includes reactive fluorescent dyes, their biological conjugates and uses.
摘要翻译：描述了与水溶性桥分子内交联的化学活性染料及其生物共轭物及其用途。具有水溶性桥梁的活性荧光染料优于光谱非交联染料或与疏水桥交联的染料的共轭物。本发明包括反应性荧光染料，它们的生物共轭物和用途。

5. 发明申请

US20100029017A1 Mono-chlorinated hydroxycoumarin conjugates 审中-公开
标题翻译：单氯化羟基香豆素共轭物
公开(公告)号：US20100029017A1
公开(公告)日：2010-02-04
申请号：US12220939
申请日：2008-07-29
申请人： Zhenjun Diwu , Timothy Dubrovsky , Barnaby Abrams , Jianjun He , Chunmei Wei , Jinfang Liao
发明人： Zhenjun Diwu , Timothy Dubrovsky , Barnaby Abrams , Jianjun He , Chunmei Wei , Jinfang Liao
IPC分类号： G01N33/536 , C07K16/18
CPC分类号： G01N33/533 , G01N33/582
摘要： The present invention provides fluorescent biopolymers that are amino acid polymers conjugated to multiple mono-chlorinated 3-carbonyl-7-hydroxy-coumarin dyes, and methods of their use. The fluorescent biopolymers containing multiple mono-chlorinated 3-carbonyl-7-hydroxy-coumarin dyes generally exhibit superior fluorescence properties compared to biopolymers labeled with multiple non-chlorinated hydroxycoumarin dyes. The fluorescent biopolymers of the invention in which the amino acid polymer is an antigen-specific antibody are particularly useful for analyzing cells by flow cytometers that are equipped with a violet laser as an excitation source due to their strong absorption at 405 nm and high fluorescence quantum yield.
摘要翻译：本发明提供荧光生物聚合物，其是与多种单氯化3-羰基-7-羟基香豆素染料缀合的氨基酸聚合物，以及它们使用的方法。与使用多种非氯化羟基香豆素染料标记的生物聚合物相比，含有多种单氯化3-羰基-7-羟基香豆素染料的荧光生物聚合物通常表现出优异的荧光特性。其中氨基酸聚合物是抗原特异性抗体的本发明的荧光生物聚合物特别可用于通过流式细胞仪分析细胞，由流式细胞仪配备紫激光作为激发源，因为它们在405nm处的强吸收和高荧光量子产量。

6. 发明授权

US6133429A Chromophores useful for the preparation of novel tandem conjugates 失效
标题翻译：用于制备新型串联共轭物的发色团
公开(公告)号：US6133429A
公开(公告)日：2000-10-17
申请号：US943491
申请日：1997-10-03
申请人： Kenneth A. Davis , James E. Bishop , Barnaby Abrams
发明人： Kenneth A. Davis , James E. Bishop , Barnaby Abrams
IPC分类号： G01N33/532 , G01N33/533 , G01N33/58 , C07K16/44
CPC分类号： G01N33/58 , G01N33/532 , G01N33/533 , Y10S436/80
摘要： The inventors herein disclose new heterobifunctional chromophores that are capable of coupling with two distinct moieties. One moiety may be either a signal-enhancing agent or a blocking agent. The second moiety may be one member of a specific binding pair. The invention is based in part on the surprising result that when a chromophore is used as a "cross-linker" between a signal-enhancing agent and a member of a binding pair (essentially being buried between the two), the signal of the chromophore is not quenched. This arrangement, wherein the chromophore acts simultaneously as a cross-linker and a detectable compound, provides significant advantages over previously known compounds since the chromophore is sterically hindered from interacting non-specifically with substances present in the test systems. Moreover, the chromophore can be used as a cross-linker with little or no loss of detectable signal.
摘要翻译：本发明人在此公开了能够与两个不同部分偶联的新的异双功能发色团。一个部分可以是信号增强剂或封闭剂。第二部分可以是特异性结合对的一个成员。本发明部分基于令人惊奇的结果，当发色团用作信号增强剂与结合对（基本上被埋在两者之间）的成员之间的“交联剂”时，发色团的信号没有淬火。这种布置，其中发色团作为交联剂和可检测化合物同时作用，提供了优于先前已知化合物的显着优点，因为发色团在空间上阻碍了非特异性地与测试系统中存在的物质相互作用。此外，发色团可以用作交联剂，几乎没有或没有可检测信号的损失。

7. 发明授权

US06372445B1 Chromophores in the preparation of novel tandem conjugates 有权
标题翻译：发色团在制备新型串联共轭物
公开(公告)号：US06372445B1
公开(公告)日：2002-04-16
申请号：US09521452
申请日：2000-03-07
申请人： Kenneth A. Davis , Barnaby Abrams , James A. Bishop
发明人： Kenneth A. Davis , Barnaby Abrams , James A. Bishop
IPC分类号： G01N3353
CPC分类号： G01N33/58 , G01N33/532 , G01N33/533 , Y10S436/80
摘要： The inventors herein disclose new heterobifunctional chromophores that are capable of coupling with two distinct moieties. One moiety may be either a signal-enhancing agent or a blocking agent. The second moiety may be one member of a specific binding pair. The invention is based in part on the surprising result that when a chromophore is used as a “cross-linker” between a signal-enhancing agent and a member of a binding pair (essentially being buried between the two), the signal of the chromophore is not quenched. This arrangement, wherein the chromophore acts simultaneously as a cross-linker and a detectable compound, provides significant advantages over previously known compounds since the chromophore is sterically hindered from interacting non-specifically with substances present in the test systems. Moreover, the chromophore can be used as a cross-linker with little or no loss of detectable signal.
摘要翻译：本发明人在此公开了能够与两个不同部分偶联的新的异双功能发色团。一个部分可以是信号增强剂或封闭剂。第二部分可以是特异性结合对的一个成员。本发明部分基于令人惊奇的结果，当发色团用作信号增强剂与结合对（基本上被埋在两者之间）的成员之间的“交联剂”时，发色团的信号没有淬火。这种布置，其中发色团作为交联剂和可检测化合物同时作用，提供了优于先前已知化合物的显着优点，因为发色团在空间上阻碍了非特异性地与测试系统中存在的物质相互作用。此外，发色团可以用作交联剂，几乎没有或没有可检测信号的损失。

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