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1. 发明授权

US5981183A Method for amplifying and detecting of target nucleic acid sequence using thermostable enzyme 失效
标题翻译：使用热稳定酶扩增和检测靶核酸序列的方法
公开(公告)号：US5981183A
公开(公告)日：1999-11-09
申请号：US821782
申请日：1997-03-20
申请人： Yutaka Takarada , Hiroaki Inoue , Shuji Shibata , Yoshihisa Kawamura
发明人： Yutaka Takarada , Hiroaki Inoue , Shuji Shibata , Yoshihisa Kawamura
IPC分类号： C12Q1/68 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/6865
摘要： In a method for amplifying the specific nucleic acid sequence, a highly specific amplification which has low possibility of non-specific hybridization can be carried out. Highly stable reagents, the activities of which do not decrease in case of supply and storage, are also provided. Thermostable enzymes are used as RNA dependent DNA polymerase, DNA dependent DNA polymerase, DNA dependent RNA polymerase and ribonuclease H, which are required for the amplification system based on replicated RNA. Especially, it is preferable that a thermostable enzyme derived from Thermus thermophilus which has RNA dependent DNA polymerase activity, DNA dependent DNA polymerase activity and ribonuclease H activity, and thermostable DNA dependent RNA polymerase are used together. By this method, inactivation of enzymes are prevented by using thermostable enzymes, and the amplification can be carried out without sequential addition of enzymes.
摘要翻译：在扩增特异性核酸序列的方法中，可以进行非特异性杂交的可能性低的高度特异性扩增。还提供了稳定的试剂，其供应和储存的活性不降低。热稳定酶用作基于复制RNA的扩增系统所需的RNA依赖性DNA聚合酶，DNA依赖性DNA聚合酶，DNA依赖性RNA聚合酶和核糖核酸酶H。特别优选共同使用具有RNA依赖性DNA聚合酶活性，DNA依赖性DNA聚合酶活性和核糖核酸酶H活性的嗜热栖热菌的热稳定性酶和耐热性DNA依赖型RNA聚合酶。通过这种方法，通过使用热稳定酶来防止酶的失活，并且可以不依次加入酶进行扩增。

2. 发明授权

US06303306B1 Method for amplifying and detecting of target nucleic acid sequence using thermostable enzyme 有权
标题翻译：使用热稳定酶扩增和检测靶核酸序列的方法
公开(公告)号：US06303306B1
公开(公告)日：2001-10-16
申请号：US09292435
申请日：1999-04-15
申请人： Yutaka Takarada , Hiroaki Inoue , Shuji Shibata , Yoshihisa Kawamura
发明人： Yutaka Takarada , Hiroaki Inoue , Shuji Shibata , Yoshihisa Kawamura
IPC分类号： C12Q168
CPC分类号： C12Q1/6865 , C12Q2527/101
摘要： In a method for amplifying the specific nucleic acid sequence, a highly specific amplification which has low possibility of non-specific hybridization can be carried out. Highly stable reagents, the activities of which do not decrease in case of supply and storage, are also provided. Thermostable enzymes are used as RNA dependent DNA polymerase, DNA dependent DNA polymerase, DNA dependent RNA polymerase and ribonuclease H, which are required for the amplification system based on replicated RNA. Especially, it is preferable that a thermostable enzyme derived from Thermus thermophilus which has RNA dependent DNA polymerase activity, DNA dependent DNA polymerase activity and ribonuclease H activity, and thermostable DNA dependent RNA polymerase are used together. By this method, inactivation of enzymes are prevented by using thermostable enzymes, and the amplification can be carried out without sequential addition of enzymes.
摘要翻译：在扩增特异性核酸序列的方法中，可以进行非特异性杂交的可能性低的高度特异性扩增。还提供了稳定的试剂，其供应和储存的活性不降低。热稳定酶用作基于复制RNA的扩增系统所需的RNA依赖性DNA聚合酶，DNA依赖性DNA聚合酶，DNA依赖性RNA聚合酶和核糖核酸酶H。特别优选共同使用具有RNA依赖性DNA聚合酶活性，DNA依赖性DNA聚合酶活性和核糖核酸酶H活性的嗜热栖热菌的热稳定性酶和耐热性DNA依赖型RNA聚合酶。通过这种方法，通过使用热稳定酶来防止酶的失活，并且可以不依次加入酶进行扩增。

3. 发明授权

US07659060B2 Method for identifying nucleotide polymorphism 有权
标题翻译：识别核苷酸多态性的方法
公开(公告)号：US07659060B2
公开(公告)日：2010-02-09
申请号：US10653586
申请日：2003-09-02
申请人： Yutaka Takarada , Yoshihiro Soya , Yoshihisa Kawamura
发明人： Yutaka Takarada , Yoshihiro Soya , Yoshihisa Kawamura
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6827 , C12Q2565/101 , C12Q2563/173
摘要： A method for identifying a nucleotide polymorphism, the method comprising hybridizing a labeled oligonucleotide for a wild type or a mutant type to a nucleic acid containing a specific nucleotide polymorphic site in a sample; allowing a nucleic acid-specific label to act thereon; detecting an interaction between the label of the oligonucleotide and the nucleic acid-specific label; and identifying a nucleotide polymorphism, wherein the identification utilizes a difference due to the nucleotide polymorphism in conditions under which the hybridized oligonucleotide is separated into a single strand.
摘要翻译：一种用于鉴定核苷酸多态性的方法，所述方法包括将野生型或突变型的标记寡核苷酸与含有样品中特定核苷酸多态性位点的核酸杂交; 允许核酸特异性标记作用于其上; 检测寡核苷酸的标记与核酸特异性标记之间的相互作用; 并鉴定核苷酸多态性，其中鉴定在杂交寡核苷酸被分离成单链的条件下利用由于核苷酸多态性引起的差异。

4. 发明授权

US06225065B1 Kit for amplifying nucleic acid 失效
标题翻译：用于扩增核酸的试剂盒
公开(公告)号：US06225065B1
公开(公告)日：2001-05-01
申请号：US09418027
申请日：1999-10-14
申请人： Masao Kitabayashi , Taku Arakawa , Hiroaki Inoue , Bunsei Kawakami , Yoshihisa Kawamura , Tadayuki Imanaka , Masahiro Takagi , Masaaki Morikawa
发明人： Masao Kitabayashi , Taku Arakawa , Hiroaki Inoue , Bunsei Kawakami , Yoshihisa Kawamura , Tadayuki Imanaka , Masahiro Takagi , Masaaki Morikawa
IPC分类号： C12Q168
CPC分类号： C12N9/1252 , C12Q1/686
摘要： A nucleic acid amplifying enzyme having a short reaction time and high fidelity is provided. The enzyme of this invention is a thermostable DNA polymerase having a nucleic acid extension rate of at least 30 bases per second and a 3′-5′ exonuclease activity. Also provided are a method and kit for amplifying nucleic acid.
摘要翻译：提供了具有短反应时间和高保真度的核酸扩增酶。本发明的酶是具有至少30个碱基/秒的核酸延伸速率和3'-5'核酸外切酶活性的热稳定的DNA聚合酶。还提供了用于扩增核酸的方法和试剂盒。

5. 发明授权

US6054301A Methods of amplification using a thermostable DNA polymerase from the hyperthermophilic archaeon strain KOD1 and reagent kit therefor 失效
标题翻译：使用来自超嗜热古细菌菌株KOD1的热稳定DNA聚合酶的扩增方法及其试剂盒
公开(公告)号：US6054301A
公开(公告)日：2000-04-25
申请号：US656005
申请日：1996-05-24
申请人： Masao Kitabayashi , Taku Arakawa , Hiroaki Inoue , Bunsei Kawakami , Yoshihisa Kawamura , Tadayuki Imanaka , Masahiro Takagi , Masaaki Morikawa
发明人： Masao Kitabayashi , Taku Arakawa , Hiroaki Inoue , Bunsei Kawakami , Yoshihisa Kawamura , Tadayuki Imanaka , Masahiro Takagi , Masaaki Morikawa
IPC分类号： C12N15/09 , C12N1/21 , C12N9/12 , C12N15/54 , C12P21/02 , C12P21/04 , C12Q1/68 , C12R1/01 , C12R1/19 , G01N33/53 , G01N33/566 , C12P19/34 , C07K15/00
CPC分类号： C12Q1/686 , C12N9/1252
摘要： A nucleic acid amplifying enzyme having a short reaction time and high fidelity is provided. The enzyme of this invention is a thermostable DNA polymerase having a nucleic acid extension rate of at least 30 bases per second and a 3'-5' exonuclease activity. Also provided are a method and kit for amplifying nucleic acid.
摘要翻译：提供了具有短反应时间和高保真度的核酸扩增酶。本发明的酶是具有至少30个碱基/秒的核酸延伸速率和3'-5'核酸外切酶活性的热稳定的DNA聚合酶。还提供了用于扩增核酸的方法和试剂盒。

6. 发明授权

US6033859A Thermostable DNA polymerase from a hyperthermophilic archaeon strain KOD1 失效
标题翻译：来自超嗜热古细菌菌株KOD1的热稳定DNA聚合酶
公开(公告)号：US6033859A
公开(公告)日：2000-03-07
申请号：US73354
申请日：1998-05-06
申请人： Masao Kitabayashi , Taku Arakawa , Hiroaki Inoue , Bunsei Kawakami , Yoshihisa Kawamura , Tadayuki Imanaka , Masahiro Takagi , Masaaki Morikawa
发明人： Masao Kitabayashi , Taku Arakawa , Hiroaki Inoue , Bunsei Kawakami , Yoshihisa Kawamura , Tadayuki Imanaka , Masahiro Takagi , Masaaki Morikawa
IPC分类号： C12N9/12 , C12Q1/68 , C12P19/34 , C07K14/00 , C12N9/00
CPC分类号： C12N9/1252 , C12Q1/686
摘要： A nucleic acid amplifying enzyme having a short reaction time and high fidelity is provided. The enzyme of this invention is a thermostable DNA polymerase having a nucleic acid extension rate of at least 30 bases per second and a 3'-5' exonuclease activity. Also provided are a method and kit for amplifying nucleic acid.
摘要翻译：提供了具有短反应时间和高保真度的核酸扩增酶。本发明的酶是具有至少30个碱基/秒的核酸延伸速率和3'-5'核酸外切酶活性的热稳定的DNA聚合酶。还提供了用于扩增核酸的方法和试剂盒。

7. 发明授权

US08419995B2 Imprint method 有权
标题翻译：印记法
公开(公告)号：US08419995B2
公开(公告)日：2013-04-16
申请号：US12563461
申请日：2009-09-21
申请人： Ikuo Yoneda , Kentaro Matsunaga , Yukiko Kikuchi , Yoshihisa Kawamura , Eishi Shiobara , Shinichi Ito , Tetsuro Nakasugi , Hirokazu Kato
发明人： Ikuo Yoneda , Kentaro Matsunaga , Yukiko Kikuchi , Yoshihisa Kawamura , Eishi Shiobara , Shinichi Ito , Tetsuro Nakasugi , Hirokazu Kato
IPC分类号： B28B11/08 , B29C35/08
CPC分类号： B29C37/0003 , B29C35/0888 , B29C37/006 , B29C59/022 , B29C2035/0822 , B29C2035/0827 , B29C2059/023 , B82Y10/00 , B82Y40/00 , G03F7/0002
摘要： An imprint method includes applying a light curable resin on a substrate to be processed, the substrate including first and second regions on which the light curable resin is applied, contacting an imprint mold with the light curable resin, curing the light curable resin by irradiating the light curable resin with light passing through the imprint mold, generating gas by performing a predetermined process to the light curable resin applied on a region of the substrate, the region including at least the first region, wherein an amount of gas generated from the light curable resin applied on the first region is larger than an amount of gas generated from the light curable resin of the second region, and forming a pattern by separating the imprint mold from the light curable resin after the gas being generated.
摘要翻译：压印方法包括将光固化树脂施加在待加工的基板上，所述基板包括施加有光固化树脂的第一和第二区域，使印模与光固化树脂接触，通过照射光固化树脂来固化光固化树脂光固化树脂，其光通过压印模具，通过对施加在基板的区域上的光固化树脂进行预定处理产生气体，该区域至少包括第一区域，其中从光可固化施加在第一区域上的树脂大于由第二区域的光固化树脂产生的气体的量，并且在产生气体之后通过从光固化树脂分离压印模具来形成图案。

8. 发明申请

US20110034029A1 PATTERN FORMATION METHOD AND METHOD FOR MANUFACTURING SEMICONDUCTOR DEVICE 失效
标题翻译：用于制造半导体器件的图案形成方法和方法
公开(公告)号：US20110034029A1
公开(公告)日：2011-02-10
申请号：US12849599
申请日：2010-08-03
申请人： Kentaro MATSUNAGA , Tomoya Oori , Eishi Shiobara , Yukiko Sato , Yoshihisa Kawamura
发明人： Kentaro MATSUNAGA , Tomoya Oori , Eishi Shiobara , Yukiko Sato , Yoshihisa Kawamura
IPC分类号： H01L21/306
CPC分类号： H01L21/0337 , B81C1/00031 , B81C2201/0149
摘要： According to one embodiment, a pattern formation method is disclosed. The method includes forming a plurality of regions on a foundation and the plurality of the regions correspond to different pattern sizes. The method includes separating each of a plurality of block copolymers from another one of the plurality of the block copolymers and segregating the each of the plurality of the block copolymers into a corresponding one of the regions. The method includes performing a phase separation of the each of the block copolymers of each of the regions. The method includes selectively removing a designated phase of each of the phase-separated block copolymers to form a pattern of the each of the block copolymers and the pattern has a different pattern size for the each of the regions.
摘要翻译：根据一个实施例，公开了图案形成方法。该方法包括在基础上形成多个区域，并且多个区域对应于不同的图案尺寸。该方法包括从多个嵌段共聚物中的另一个嵌段共聚物中分离多个嵌段共聚物，并将多个嵌段共聚物中的每一个分离成相应的一个区域。该方法包括进行每个区域的每个嵌段共聚物的相分离。该方法包括选择性地除去每个相分离的嵌段共聚物的指定相以形成每个嵌段共聚物的图案，并且该图案对于每个区域具有不同的图案尺寸。

9. 发明授权

US07422882B2 Modified thermostable DNA polymerase 有权
标题翻译：修饰的热稳定DNA聚合酶
公开(公告)号：US07422882B2
公开(公告)日：2008-09-09
申请号：US09852922
申请日：2001-05-10
申请人： Toshihiro Kuroita , Masao Kitabayashi , Yoshikazu Ishida , Hideyuki Komatsubara , Yoshiaki Nishiya , Bunsei Kawakami , Yoshihisa Kawamura , Tadayuki Imanaka
发明人： Toshihiro Kuroita , Masao Kitabayashi , Yoshikazu Ishida , Hideyuki Komatsubara , Yoshiaki Nishiya , Bunsei Kawakami , Yoshihisa Kawamura , Tadayuki Imanaka
IPC分类号： C12N9/12 , C12P19/34
CPC分类号： C12N9/1252
摘要： An object of the present invention is to provide a thermostable DNA polymerase with enhanced amplification efficiency and/or improved fidelity in polymerase chain reaction (PCR), and provide a process for production thereof. More specifically, the present invention provides thermostable DNA polymerase wherein in the DX1EX2X3X4H sequence (D: aspartic acid, E: glutamic acid, H: histidine, X1, X2, X3 and X4: any amino acid) consisting of DX1E sequence within the EXO I region and a four amino acid length peptide adjacent to said glutamic acid(E) of thermostable DNA polymerase having 3′-5′ exonuclease activity, histidine(H) has been replaced by another amino acid.
摘要翻译：本发明的目的是提供具有增强的扩增效率和/或聚合酶链反应（PCR）中的改进的保真度的热稳定性DNA聚合酶，并提供其生产方法。更具体地说，本发明提供了热稳定的DNA聚合酶，其中在X 1，X 2，X 3，X 4，序列（D：天冬氨酸，E：谷氨酸，H：组氨酸，X 1，X 2，X 3和X 3，由SEQ ID NO：1区域内的DX1E序列组成的4个氨基酸长度肽与邻近所述谷氨酸（E）的4个氨基酸长度的肽组成，所述热稳定性DNA聚合酶具有3' -5'核酸外切酶活性，组氨酸（H）已被另一种氨基酸取代。

10. 发明授权

US07384739B2 Compositions for enhancing DNA synthesis, DNA polymerase-related factors and utilization thereof 有权
标题翻译：用于增强DNA合成，DNA聚合酶相关因子及其利用的组合物
公开(公告)号：US07384739B2
公开(公告)日：2008-06-10
申请号：US10495581
申请日：2002-11-14
申请人： Masao Kitabayashi , Toshihiro Kuroita , Yoshikazu Ishida , Hideyuki Komatsubara , Yoshiaki Nishiya , Masanori Oka , Yoshihisa Kawamura , Tadayuki Imanaka
发明人： Masao Kitabayashi , Toshihiro Kuroita , Yoshikazu Ishida , Hideyuki Komatsubara , Yoshiaki Nishiya , Masanori Oka , Yoshihisa Kawamura , Tadayuki Imanaka
IPC分类号： C12Q1/68 , C12P19/34 , C12N9/00 , C08B3/00
CPC分类号： C07K14/195 , C12N9/1252 , C12Q1/6846 , C12Q2527/125 , C12Q2521/101
摘要： The invention provides methods, kits, and compositions for enhancing synthesis of DNA involving a carboxylate ion-supplying substance that is effective in promoting DNA synthesis in enzymatic DNA synthesis reactions. The invention further provides a thermostable DNA polymerase-related factor derived from Thermococcus species, which has an activity to promote the DNA synthesis activity of DNA polymerase or which binds to DNA polymerase.
摘要翻译：本发明提供用于增强DNA合成的方法，试剂盒和组合物，所述方法，试剂盒和组合物涉及在酶促DNA合成反应中有效促进DNA合成的羧酸根离子供应物质。本发明还提供了源自Thermococcus种的热稳定DNA聚合酶相关因子，其具有促进DNA聚合酶的DNA合成活性或与DNA聚合酶结合的活性。

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