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1. 发明授权

US08512987B2 Method of producing L-amino acid 有权
标题翻译：生产L-氨基酸的方法
公开(公告)号：US08512987B2
公开(公告)日：2013-08-20
申请号：US12545966
申请日：2009-08-24
申请人： Yuri Nagai , Kazuyuki Hayashi , Takuji Ueda , Yoshihiro Usuda , Kazuhiko Matsui
发明人： Yuri Nagai , Kazuyuki Hayashi , Takuji Ueda , Yoshihiro Usuda , Kazuhiko Matsui
IPC分类号： C12P13/24
CPC分类号： C12P13/08 , C12P13/04
摘要： An L-amino acid is produced by culturing a microorganism belonging to the family Enterobacteriaceae having an L-amino acid-producing ability and modified so that glycerol dehydrogenase and dihydroxyacetone kinase activities are increased, in a medium containing glycerol as a carbon source to produce and accumulate an L-amino acid in the medium or cells, and collecting the L-amino acid from the medium or the cells.
摘要翻译：通过在含有甘油作为碳源的培养基中培养属于具有L-氨基酸生产能力的肠杆菌科的微生物并进行修饰以使甘油脱氢酶和二羟基丙酮激酶活性增加的L-氨基酸产生，在培养基或细胞中积累L-氨基酸，并从培养基或细胞中收集L-氨基酸。

2. 发明申请

US20100047878A1 METHOD OF PRODUCING L-AMINO ACID 有权
标题翻译：生产L-氨基酸的方法
公开(公告)号：US20100047878A1
公开(公告)日：2010-02-25
申请号：US12545966
申请日：2009-08-24
申请人： Yuri Nagai , Kazuyuki Hayashi , Takuji Ueda , Yoshihiro Usuda , Kazuhiko Matsui
发明人： Yuri Nagai , Kazuyuki Hayashi , Takuji Ueda , Yoshihiro Usuda , Kazuhiko Matsui
IPC分类号： C12P13/24 , C12P13/04 , C12P13/14 , C12P13/08 , C12P13/06 , C12P13/22 , C12P13/12 , C12P13/10 , C12P13/20
CPC分类号： C12P13/08 , C12P13/04
摘要： An L-amino acid is produced by culturing a microorganism belonging to the family Enterobacteriaceae having an L-amino acid-producing ability and modified so that glycerol dehydrogenase and dihydroxyacetone kinase activities are increased, in a medium containing glycerol as a carbon source to produce and accumulate an L-amino acid in the medium or cells, and collecting the L-amino acid from the medium or the cells.
摘要翻译：通过在含有甘油作为碳源的培养基中培养属于具有L-氨基酸生产能力的肠杆菌科的微生物并进行修饰以使甘油脱氢酶和二羟基丙酮激酶活性增加的L-氨基酸产生，在培养基或细胞中积累L-氨基酸，并从培养基或细胞中收集L-氨基酸。

3. 发明授权

US08030036B2 Method for producing L-lysine or L-threonine 有权
标题翻译：生产L-赖氨酸或L-苏氨酸的方法
公开(公告)号：US08030036B2
公开(公告)日：2011-10-04
申请号：US11877726
申请日：2007-10-24
申请人： Stephen Van Dien , Shintaro Iwatani , Yoshihiro Usuda , Kazuhiko Matsui , Yuta Nakai , Tomoko Suzuki , Mika Moriya , Yuichiro Tsuji , Takuji Ueda
发明人： Stephen Van Dien , Shintaro Iwatani , Yoshihiro Usuda , Kazuhiko Matsui , Yuta Nakai , Tomoko Suzuki , Mika Moriya , Yuichiro Tsuji , Takuji Ueda
IPC分类号： C12P13/08 , C12N9/04 , C12N1/21 , C07H21/04
CPC分类号： C12N9/0006 , C12P13/08
摘要： A bacterium belonging to the genus Escherichia which has an ability to produce L-lysine or L-threonine and which is modified so that a malic enzyme does not function normally in a cell, and a method for producing L-lysine or L-threonine, comprising culturing the bacterium in a medium to produce and cause accumulation of L-lysine or L-threonine, and collecting the L-lysine or L-threonine from the medium.
摘要翻译：属于埃希氏菌属的细菌，其具有产生L-赖氨酸或L-苏氨酸的能力，并且其被修饰以使得苹果酸在细胞中不起作用;以及L-赖氨酸或L-苏氨酸的制备方法，包括在培养基中培养细菌以产生并引起L-赖氨酸或L-苏氨酸的积累，并从培养基中收集L-赖氨酸或L-苏氨酸。

4. 发明授权

US07306933B2 Method for producing L-lysine or L-threonine 有权
标题翻译：生产L-赖氨酸或L-苏氨酸的方法
公开(公告)号：US07306933B2
公开(公告)日：2007-12-11
申请号：US11275437
申请日：2006-01-03
申请人： Stephen Van Dien , Shintaro Iwatani , Yoshihiro Usuda , Kazuhiko Matsui , Yuta Nakai , Tomoko Suzuki , Mika Moriya , Yuichiro Tsuji , Takuji Ueda
发明人： Stephen Van Dien , Shintaro Iwatani , Yoshihiro Usuda , Kazuhiko Matsui , Yuta Nakai , Tomoko Suzuki , Mika Moriya , Yuichiro Tsuji , Takuji Ueda
IPC分类号： C12P13/08 , C12N9/04 , C12N1/21 , C07H21/04
CPC分类号： C12N9/0006 , C12P13/08
摘要： A bacterium belonging to the genus Escherichia which has an ability to produce L-lysine or L-threonine and which is modified so that a malic enzyme does not function normally in a cell, and a method for producing L-lysine or L-threonine, comprising culturing the bacterium in a medium to produce and cause accumulation of L-lysine or L-threonine, and collecting the L-lysine or L-threonine from the medium.
摘要翻译：属于埃希氏菌属的细菌，其具有产生L-赖氨酸或L-苏氨酸的能力，并且其被修饰以使得苹果酸在细胞中不起作用;以及L-赖氨酸或L-苏氨酸的制备方法，包括在培养基中培养细菌以产生并引起L-赖氨酸或L-苏氨酸的积累，并从培养基中收集L-赖氨酸或L-苏氨酸。

5. 发明申请

US20060154289A1 Method for determining metabolic flux affecting substance production 有权
标题翻译：测定代谢通量影响物质生产的方法
公开(公告)号：US20060154289A1
公开(公告)日：2006-07-13
申请号：US11337580
申请日：2006-01-24
申请人： Stephen Dien , Shintaro Iwatani , Yoshihiro Usuda , Kazuhiko Matsui , Takuji Ueda , Yuichiro Tsuji
发明人： Stephen Dien , Shintaro Iwatani , Yoshihiro Usuda , Kazuhiko Matsui , Takuji Ueda , Yuichiro Tsuji
IPC分类号： C12Q1/68 , C12Q1/18 , C12N1/21
CPC分类号： C12N9/0006 , C12P13/08
摘要： A metabolic flux affecting substance production using cells is determined by 1) creating a stoichiometric matrix based on formulas of biochemical reactions from a substrate through a desired produced substance, 2) selecting the same number of independent metabolic fluxes from all metabolic fluxes as the degree of freedom of the stoichiometric matrix as free fluxes, 3) creating a sufficient number of random combinations of the free fluxes for a statistical analysis and calculating a metabolic flux distribution from each created combination based on the stoichiometric matrix, 4) obtaining a regression equation including a minimum number of free fluxes that shows a correlation with substance production from the calculated metabolic flux distributions by a multivariate statistical analysis, and 5) determining at least one metabolic flux affecting substance production based on a coefficient in the obtained regression equation.
摘要翻译：影响使用细胞的物质生产的代谢通量是通过以下方法确定的：1）根据基质通过所需生产物质的生物化学反应的公式产生化学计量矩阵，2）从所有代谢通量中选择相同数量的独立代谢通量，作为自由通量的化学计量矩阵的自由度，3）产生足够数量的自由通量的随机组合用于统计分析，并且基于化学计量矩阵计算每个创建的组合的代谢通量分布，4）获得包括通过多变量统计分析从计算的代谢通量分布中显示与物质生产相关的最小数量的自由通量，以及5）基于所获得的回归方程中的系数确定影响物质生产的至少一种代谢通量。

6. 发明授权

US07809511B2 Method for determining metabolic flux affecting substance production 有权
标题翻译：测定代谢通量影响物质生产的方法
公开(公告)号：US07809511B2
公开(公告)日：2010-10-05
申请号：US11337580
申请日：2006-01-24
申请人： Stephen Van Dien , Shintaro Iwatani , Yoshihiro Usuda , Kazuhiko Matsui , Takuji Ueda , Yuichiro Tsuji
发明人： Stephen Van Dien , Shintaro Iwatani , Yoshihiro Usuda , Kazuhiko Matsui , Takuji Ueda , Yuichiro Tsuji
IPC分类号： G06F19/00
CPC分类号： C12N9/0006 , C12P13/08
摘要： A metabolic flux affecting substance production using cells is determined by 1) creating a stoichiometric matrix based on formulas of biochemical reactions from a substrate through a desired produced substance, 2) selecting the same number of independent metabolic fluxes from all metabolic fluxes as the degree of freedom of the stoichiometric matrix as free fluxes, 3) creating a sufficient number of random combinations of the free fluxes for a statistical analysis and calculating a metabolic flux distribution from each created combination based on the stoichiometric matrix, 4) obtaining a regression equation including a minimum number of free fluxes that shows a correlation with substance production from the calculated metabolic flux distributions by a multivariate statistical analysis, and 5) determining at least one metabolic flux affecting substance production based on a coefficient in the obtained regression equation.
摘要翻译：影响使用细胞的物质生产的代谢通量是通过以下方法确定的：1）根据基质通过所需生产物质的生物化学反应的公式产生化学计量矩阵，2）从所有代谢通量中选择相同数量的独立代谢通量，作为自由通量的化学计量矩阵的自由度，3）产生足够数量的自由通量的随机组合用于统计分析，并且基于化学计量矩阵计算每个创建的组合的代谢通量分布，4）获得包括通过多变量统计分析从计算的代谢通量分布中显示与物质生产相关的最小数量的自由通量，以及5）基于所获得的回归方程中的系数确定影响物质生产的至少一种代谢通量。

7. 发明申请

US20090148915A1 Method for Producing L-Lysine or L-Threonine 有权
标题翻译：生产L-赖氨酸或L-苏氨酸的方法
公开(公告)号：US20090148915A1
公开(公告)日：2009-06-11
申请号：US11877726
申请日：2007-10-24
申请人： Stephen Van Dien , Shintaro Iwatani , Yoshihiro Usuda , Kazuhiko Matsui , Yuta Nakai , Tomoko Suzuki , Mika Moriya , Yuichiro Tsuji , Takuji Ueda
发明人： Stephen Van Dien , Shintaro Iwatani , Yoshihiro Usuda , Kazuhiko Matsui , Yuta Nakai , Tomoko Suzuki , Mika Moriya , Yuichiro Tsuji , Takuji Ueda
IPC分类号： C12P13/08
CPC分类号： C12N9/0006 , C12P13/08
摘要： A bacterium belonging to the genus Escherichia which has an ability to produce L-lysine or L-threonine and which is modified so that a malic enzyme does not function normally in a cell, and a method for producing L-lysine or L-threonine, comprising culturing the bacterium in a medium to produce and cause accumulation of L-lysine or L-threonine, and collecting the L-lysine or L-threonine from the medium.
摘要翻译：属于埃希氏菌属的细菌，其具有产生L-赖氨酸或L-苏氨酸的能力，并且其被修饰以使得苹果酸在细胞中不起作用;以及L-赖氨酸或L-苏氨酸的制备方法，包括在培养基中培养细菌以产生并引起L-赖氨酸或L-苏氨酸的积累，并从培养基中收集L-赖氨酸或L-苏氨酸。

8. 发明申请

US20060154344A1 METHOD FOR PRODUCING L-LYSINE OR L-THREONINE 有权
标题翻译： L-赖氨酸或L-噻吩的生产方法
公开(公告)号：US20060154344A1
公开(公告)日：2006-07-13
申请号：US11275437
申请日：2006-01-03
申请人： Stephen Van Dien , Shintaro Iwatani , Yoshihiro Usuda , Kazuhiko Matsui , Yuta Nakai , Tomoko Suzuki , Mika Moriya , Yuichiro Tsuji , Takuji Ueda
发明人： Stephen Van Dien , Shintaro Iwatani , Yoshihiro Usuda , Kazuhiko Matsui , Yuta Nakai , Tomoko Suzuki , Mika Moriya , Yuichiro Tsuji , Takuji Ueda
IPC分类号： C12P13/08 , C12N1/21 , C12N15/74
CPC分类号： C12N9/0006 , C12P13/08
摘要： A bacterium belonging to the genus Escherichia which has an ability to produce L-lysine or L-threonine and which is modified so that a malic enzyme does not function normally in a cell, and a method for producing L-lysine or L-threonine, comprising culturing the bacterium in a medium to produce and cause accumulation of L-lysine or L-threonine, and collecting the L-lysine or L-threonine from the medium.
摘要翻译：属于埃希氏菌属的细菌，其具有产生L-赖氨酸或L-苏氨酸的能力，并且其被修饰以使得苹果酸在细胞中不起作用;以及L-赖氨酸或L-苏氨酸的制备方法，包括在培养基中培养细菌以产生并引起L-赖氨酸或L-苏氨酸的积累，并从培养基中收集L-赖氨酸或L-苏氨酸。

9. 发明授权

US07833761B2 Amino acid producing microorganism and a method for producing an amino acid 有权
标题翻译：氨基酸生产微生物和氨基酸的制备方法
公开(公告)号：US07833761B2
公开(公告)日：2010-11-16
申请号：US12202476
申请日：2008-09-02
申请人： Masaru Terashita , Yoshihiro Usuda , Kazuhiko Matsui
发明人： Masaru Terashita , Yoshihiro Usuda , Kazuhiko Matsui
IPC分类号： C12P13/04 , C12P13/06 , C12P13/08 , C12P13/22
CPC分类号： C12N9/0008 , C12P13/06 , C12P13/08 , C12P13/222 , C12P13/227
摘要： A microorganism is provided which has an ability to produce an L-amino acid such as L-lysine, L-tryptophan, L-phenylalanine, L-valine, L-leucine, L-isoleucine and L-serine, and has been modified to increase the activity of pyruvate synthase or pyruvate:NADP+ oxidoreductase. This microorganism is cultured in a medium containing ethanol or an aliphatic acid as the carbon source to produce and accumulate the L-amino acid in the medium or cells, and the L-amino acid is collected from the medium or the cells.
摘要翻译：提供了具有产生L-赖氨酸，L-色氨酸，L-苯丙氨酸，L-缬氨酸，L-亮氨酸，L-异亮氨酸，L-丝氨酸等L-氨基酸的能力的微生物，增加丙酮酸合酶或丙酮酸：NADP +氧化还原酶的活性。将该微生物在含有乙醇或脂肪酸作为碳源的培养基中培养，以在培养基或细胞中产生并积累L-氨基酸，从培养基或细胞收集L-氨基酸。

10. 发明申请

US20100081180A1 METHOD FOR PRODUCING AN ORGANIC ACID 有权
标题翻译：生产有机酸的方法
公开(公告)号：US20100081180A1
公开(公告)日：2010-04-01
申请号：US12579594
申请日：2009-10-15
申请人： Keita Fukui , Yoshinori Tajima , Kazue Kawamura , Yoshihiro Usuda , Kazuhiko Matsui
发明人： Keita Fukui , Yoshinori Tajima , Kazue Kawamura , Yoshihiro Usuda , Kazuhiko Matsui
IPC分类号： C12P7/46 , C12P1/04
CPC分类号： C12P7/46
摘要： An organic acid is produced by allowing a bacterium which has an ability to produce an organic acid and has been modified so that expression of yidE gene is enhanced, or a product obtained by processing the bacterium, to act on an organic raw material in a reaction mixture containing carbonate ions, bicarbonate ions, or carbon dioxide gas to produce the organic acid, and collecting the organic acid.
摘要翻译：有机酸是通过使具有生产有机酸的能力的细菌进行改性的，以使yidE基因的表达增强，或通过加工细菌获得的产物作用于反应中的有机原料含有碳酸根离子，碳酸氢根离子或二氧化碳气体的混合物以产生有机酸，并收集有机酸。

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