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    • 9. 发明申请
    • Methods And Compositions For Targeting Sequences Of Interest To The Chloroplast
    • 用于靶向叶绿体感兴趣的方法和组合物
    • US20120042412A1
    • 2012-02-16
    • US13208960
    • 2011-08-12
    • Henrik AlbertLinda A. CastleMatthew HeckertJian LuDaniel L. SiehlYumin Tao
    • Henrik AlbertLinda A. CastleMatthew HeckertJian LuDaniel L. SiehlYumin Tao
    • A01H1/06C12N15/82C12N5/10C12N9/96A01H5/10C07K19/00C12N9/02C12N15/62A01H5/00
    • C12N15/8221C12N9/0069C12N15/82C12N15/8274C12Y113/11027
    • Chimeric polynucleotides comprising a nucleotide sequence encoding a chloroplast transit peptide operably linked to a heterologous polynucleotide of interest are provided, wherein the chloroplast transit peptide comprises an amino acid sequence having the chloroplast transit peptide sequence as set forth in SEQ ID NO:1 or a biologically active variant or fragment thereof or wherein the chloroplast transit peptide comprises the sequence set forth in SEQ ID NO: 58 or an active variant or fragment thereof. Chimeric polypeptides encoding the same, as well as, cells, plant cells, plants and seeds are further provided which comprise the chimeric polynucleotides. Compositions further include HPPD polypeptides and polynucleotides encoding the same as set forth in SEQ ID NOS: 57 and 60 or active variants and fragments thereof. Such sequences comprise the chloroplast transit peptide as set forth in SEQ ID NO: 58 or an active variants or fragments thereof. Cells, plant cells, plants and seeds are further provided which comprise such sequences. Methods of use of the various sequences are also provided.
    • 提供了包含编码与目标异源多核苷酸有效连接的叶绿体转运肽的核苷酸序列的嵌合多核苷酸,其中叶绿体转运肽包含具有如SEQ ID NO:1所示的叶绿体转运肽序列的氨基酸序列或生物学上 活性变体或其片段,或其中叶绿体转运肽包含SEQ ID NO:58所示的序列或其活性变体或片段。 还提供了编码其的嵌合多肽,以及细胞,植物细胞,植物和种子,其包含嵌合多核苷酸。 组合物还包括编码与SEQ ID NO:57和60所示相同的HPPD多肽和多核苷酸或其活性变体和片段。 这样的序列包含如SEQ ID NO:58所示的叶绿体转运肽或其活性变体或片段。 进一步提供包含这些序列的细胞,植物细胞,植物和种子。 还提供了使用各种序列的方法。