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1. 发明申请

US20080047031A1 Novel FRT recombination sites and methods of use 有权
标题翻译：新型FRT重组位点和使用方法
公开(公告)号：US20080047031A1
公开(公告)日：2008-02-21
申请号：US11487300
申请日：2006-07-14
申请人： Yumin Tao , Dennis Bidney , William Gordon-Kamm , Leszek Lyznik
发明人： Yumin Tao , Dennis Bidney , William Gordon-Kamm , Leszek Lyznik
IPC分类号： C12N15/09 , C07H21/00 , C12N5/04 , C40B40/02 , C12N5/10 , C12N1/00
CPC分类号： C12N15/8213 , C12N15/102 , C12N15/1051 , C12N15/1093 , C40B40/08
摘要： Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest, including methods to recombine polynucleotides, assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, and identify and/or characterize transcriptional regulating regions are also provided.
摘要翻译：提供了使用随机修饰的FRT重组位点群的方法和组合来鉴定，分离和/或表征修饰的FRT重组位点。重组生成修饰的FRT重组位点可用于多种感兴趣多核苷酸靶向重组方法，包括重组多核苷酸的方法，评估启动子活性，直接选择转化的生物体，最小化或消除由随机整合到基因组中的表达生物体如植物，去除感兴趣的多核苷酸，组合多个转移盒，反转或切割多核苷酸，以及鉴定和/或表征转录调节区。

2. 发明申请

US20070015195A1 Modified FRT recombination site libraries and methods of use 审中-公开
标题翻译：修改的FRT重组位点文库和使用方法
公开(公告)号：US20070015195A1
公开(公告)日：2007-01-18
申请号：US11487273
申请日：2006-07-14
申请人： Yumin Tao , Dennis Bidney , William Gordon-Kamm , Leszek Lyznik
发明人： Yumin Tao , Dennis Bidney , William Gordon-Kamm , Leszek Lyznik
IPC分类号： C40B30/06 , C40B50/06
CPC分类号： C12N15/8213 , C12N15/102 , C12N15/1051 , C12N15/1093 , C40B40/08
摘要： Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. Kits comprising the library populations of FRT sites are also provided, as are methods to make a library of modified FRT recombination sites. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest.
摘要翻译：提供了使用随机修饰的FRT重组位点群的方法和组合来鉴定，分离和/或表征修饰的FRT重组位点。还提供了包含FRT位点的文库群的试剂盒，以及制备修饰的FRT重组位点的文库的方法。重组生成修饰的FRT重组位点可用于多种目标多重核苷酸重组方法。

3. 发明申请

US20050034193A1 Transcriptional activator nucleic acids, polypeptides and methods of use thereof 有权
标题翻译：转录激活剂核酸，多肽及其使用方法
公开(公告)号：US20050034193A1
公开(公告)日：2005-02-10
申请号：US10744620
申请日：2003-12-22
申请人： Keith Lowe , William Gordon-Kamm , Theodore Klein , Sonriza Rasco-Gaunt , Rebecca Cahoon , Xifan Sun , George Hoerster , Carolyn Gregory , Ramgopal Nadimpalli , Yumin Tao
发明人： Keith Lowe , William Gordon-Kamm , Theodore Klein , Sonriza Rasco-Gaunt , Rebecca Cahoon , Xifan Sun , George Hoerster , Carolyn Gregory , Ramgopal Nadimpalli , Yumin Tao
IPC分类号： C07K14/415 , C12N15/29 , C12N15/82 , C12P21/00
CPC分类号： C07K14/415 , C12N15/8209 , C12N15/8261 , C12N15/8287 , Y02A40/146
摘要： The invention provides isolated nucleic acids and their encoded proteins which act as transcriptional activators and methods of use thereof. The invention further provides expression cassettes, transformed host cells, transgenic plants and plant parts, and antibody compositions.
摘要翻译：本发明提供分离的核酸及其编码的作为转录激活子的蛋白质及其使用方法。本发明进一步提供表达盒，转化的宿主细胞，转基因植物和植物部分以及抗体组合物。

4. 发明申请

US20050148765A1 Plant cell cyclin genes 审中-公开
标题翻译：植物细胞周期蛋白基因
公开(公告)号：US20050148765A1
公开(公告)日：2005-07-07
申请号：US10814492
申请日：2004-03-31
申请人： Rebecca Cahoon , Karlene Butler , William Gordon-Kamm , Keith Lowe , Yumin Tao , Emil Orozco , Theodore Klein , Joan Odell
发明人： Rebecca Cahoon , Karlene Butler , William Gordon-Kamm , Keith Lowe , Yumin Tao , Emil Orozco , Theodore Klein , Joan Odell
IPC分类号： C07K14/415 , C12N15/82 , C07H21/04 , A01H1/00 , C12Q1/68 , C12N5/04
CPC分类号： C12N15/8261 , C07K14/415 , C12N15/8201 , Y02A40/146
摘要： This invention relates to an isolated nucleic acid fragment encoding a cyclin protein. The invention also relates to the construction of a chimeric gene encoding all or a portion of the cyclin protein, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the cyclin protein in a transformed host cell.
摘要翻译：本发明涉及编码细胞周期蛋白的分离的核酸片段。本发明还涉及在正义或反义方向上编码全部或部分细胞周期蛋白的嵌合基因的构建，其中嵌合基因的表达导致转化的宿主细胞中细胞周期蛋白的水平发生变化。

5. 发明申请

US20070271628A1 Wuschel (WUS) Gene Homologs 审中-公开
公开(公告)号：US20070271628A1
公开(公告)日：2007-11-22
申请号：US11754389
申请日：2007-05-29
申请人： Keith Lowe , Rebecca Cahoon , Christopher Scelonge , Yumin Tao , William Gordon-Kamm , Wesley Bruce , Lisa Newman
发明人： Keith Lowe , Rebecca Cahoon , Christopher Scelonge , Yumin Tao , William Gordon-Kamm , Wesley Bruce , Lisa Newman
IPC分类号： A01H5/00 , C07H21/00 , C07H21/04 , C12N15/82 , C12N5/02
CPC分类号： C12N15/8241 , C07K14/415 , C12N15/62 , C12N15/8201 , C12N15/8207 , C12N15/8261 , Y02A40/146
摘要： This invention relates to isolated polynucleotides encoding WUS polypeptides. The invention further provides isolated WUS polypeptides. The invention also provides methods of using the polynucleotides to modulate the level of WUS, improve transformation efficiency, to stimulate plant cell growth, including stem cells, to stimulate organogenesis, to stimulate somatic embryogenesis, to induce apomixis, and to provide a positive selection for cells comprising the polynucleotide. The invention also relates to cells, plants and seeds comprising the polynucleotides of the invention or produced by the methods of the invention.

6. 发明申请

US20050076404A1 Cell cycle polynucleotides, polypeptides and uses thereof 审中-公开
公开(公告)号：US20050076404A1
公开(公告)日：2005-04-07
申请号：US10914311
申请日：2004-08-09
申请人： Yumin Tao , William Gordon-Kamm , Keith Lowe , Matthew Bailey
发明人： Yumin Tao , William Gordon-Kamm , Keith Lowe , Matthew Bailey
IPC分类号： C07K14/415 , C12N15/82 , A01H1/00 , C07H21/04 , C12N9/10 , C12N5/04
CPC分类号： C12N15/8261 , C07K14/415 , C12N15/8201 , Y02A40/146
摘要： The invention provides isolated polynucleotides and their encoded proteins that are involved in cell cycle regulation. The invention further provides vectors, recombinant expression cassettes, host cells, transgenic plants, and antibody compositions. The present invention provides methods and compositions relating to altering cell cycle protein content and/or composition of plants.

7. 发明申请

US20050015833A1 Method for delivery of proteins to plant cells 有权
标题翻译：将蛋白质递送到植物细胞的方法
公开(公告)号：US20050015833A1
公开(公告)日：2005-01-20
申请号：US10913202
申请日：2004-08-06
申请人： Matthew Bailey , Stanton Gelvin , Saikat Bhattacharjee , William Gordon-Kamm , Keith Lowe , Yumin Tao
发明人： Matthew Bailey , Stanton Gelvin , Saikat Bhattacharjee , William Gordon-Kamm , Keith Lowe , Yumin Tao
IPC分类号： C07H21/04 , C12N5/02 , C12N15/62 , C12N15/82 , C12N15/87
CPC分类号： C12N15/62 , C07H21/04 , C07K2319/09 , C07K2319/10 , C07K2319/61 , C07K2319/80 , C12N15/8205
摘要： The invention relates to the use of a modified Agrobacterium to deliver proteins directly to plant cells. Proteins of interest are delivered to the plant host in the form of a fusion protein with the Agrobacterium virulence protein VirF. Nucleotide sequences encoding such fusion proteins of VirF and a protein of interest are provided. Also provided are bacteria modified to comprise such fusion proteins of VirF and a protein of interest. Methods of introducing such fusion proteins into a plant host are provided. The invention finds use in facilitating plant transformation and particularly in the bio-engineering of desirable traits into crop plants.
摘要翻译：本发明涉及使用改良的农杆菌将蛋白质直接递送至植物细胞。感兴趣的蛋白质以与农杆菌毒力蛋白VirF融合蛋白的形式递送至植物宿主。提供编码VirF和感兴趣蛋白质的融合蛋白的核苷酸序列。还提供了细菌修饰以包含VirF和感兴趣的蛋白质的这种融合蛋白。提供了将这种融合蛋白引入植物宿主的方法。本发明用于促进植物转化，特别是在对作物植物期望的性状的生物工程中。

8. 发明授权

US08586361B2 FRT recombination sites and methods of use 有权
标题翻译： FRT重组位点和使用方法
公开(公告)号：US08586361B2
公开(公告)日：2013-11-19
申请号：US12893418
申请日：2010-09-29
申请人： Yumin Tao , Dennis L. Bidney , William J. Gordon-Kamm , Leszek A. Lyznik
发明人： Yumin Tao , Dennis L. Bidney , William J. Gordon-Kamm , Leszek A. Lyznik
IPC分类号： C12N15/87 , C12N15/82
CPC分类号： C12N15/8213 , C12N15/102 , C12N15/1051 , C12N15/1093 , C40B40/08
摘要： Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest, including methods to recombine polynucleotides, assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, and identify and/or characterize transcriptional regulating regions are also provided.
摘要翻译：提供了使用随机修饰的FRT重组位点群的方法和组合来鉴定，分离和/或表征修饰的FRT重组位点。重组生成修饰的FRT重组位点可用于多种感兴趣多核苷酸靶向重组方法，包括重组多核苷酸的方法，评估启动子活性，直接选择转化的生物体，最小化或消除由随机整合到基因组中的表达生物体如植物，去除感兴趣的多核苷酸，组合多个转移盒，反转或切割多核苷酸，以及鉴定和/或表征转录调节区。

9. 发明申请

US20120042412A1 Methods And Compositions For Targeting Sequences Of Interest To The Chloroplast 有权
标题翻译：用于靶向叶绿体感兴趣的方法和组合物
公开(公告)号：US20120042412A1
公开(公告)日：2012-02-16
申请号：US13208960
申请日：2011-08-12
申请人： Henrik Albert , Linda A. Castle , Matthew Heckert , Jian Lu , Daniel L. Siehl , Yumin Tao
发明人： Henrik Albert , Linda A. Castle , Matthew Heckert , Jian Lu , Daniel L. Siehl , Yumin Tao
IPC分类号： A01H1/06 , C12N15/82 , C12N5/10 , C12N9/96 , A01H5/10 , C07K19/00 , C12N9/02 , C12N15/62 , A01H5/00
CPC分类号： C12N15/8221 , C12N9/0069 , C12N15/82 , C12N15/8274 , C12Y113/11027
摘要： Chimeric polynucleotides comprising a nucleotide sequence encoding a chloroplast transit peptide operably linked to a heterologous polynucleotide of interest are provided, wherein the chloroplast transit peptide comprises an amino acid sequence having the chloroplast transit peptide sequence as set forth in SEQ ID NO:1 or a biologically active variant or fragment thereof or wherein the chloroplast transit peptide comprises the sequence set forth in SEQ ID NO: 58 or an active variant or fragment thereof. Chimeric polypeptides encoding the same, as well as, cells, plant cells, plants and seeds are further provided which comprise the chimeric polynucleotides. Compositions further include HPPD polypeptides and polynucleotides encoding the same as set forth in SEQ ID NOS: 57 and 60 or active variants and fragments thereof. Such sequences comprise the chloroplast transit peptide as set forth in SEQ ID NO: 58 or an active variants or fragments thereof. Cells, plant cells, plants and seeds are further provided which comprise such sequences. Methods of use of the various sequences are also provided.
摘要翻译：提供了包含编码与目标异源多核苷酸有效连接的叶绿体转运肽的核苷酸序列的嵌合多核苷酸，其中叶绿体转运肽包含具有如SEQ ID NO：1所示的叶绿体转运肽序列的氨基酸序列或生物学上活性变体或其片段，或其中叶绿体转运肽包含SEQ ID NO：58所示的序列或其活性变体或片段。还提供了编码其的嵌合多肽，以及细胞，植物细胞，植物和种子，其包含嵌合多核苷酸。组合物还包括编码与SEQ ID NO：57和60所示相同的HPPD多肽和多核苷酸或其活性变体和片段。这样的序列包含如SEQ ID NO：58所示的叶绿体转运肽或其活性变体或片段。进一步提供包含这些序列的细胞，植物细胞，植物和种子。还提供了使用各种序列的方法。

10. 发明申请

US20110258741A1 WUSCHEL(WUS) GENE HOMOLOGS 有权
标题翻译： WUSCHEL（WUS）基因同源物
公开(公告)号：US20110258741A1
公开(公告)日：2011-10-20
申请号：US13168133
申请日：2011-06-24
申请人： Rebecca E. Cahoon , Keith S. Lowe , William J. Gordon-Kamm , Yumin Tao , Christopher J. Scelonge
发明人： Rebecca E. Cahoon , Keith S. Lowe , William J. Gordon-Kamm , Yumin Tao , Christopher J. Scelonge
IPC分类号： A01H5/00 , A01H5/10 , C12N15/82 , C12N15/29
CPC分类号： C12N15/8241 , C07K14/415 , C12N15/62 , C12N15/8201 , C12N15/8207 , C12N15/8261 , Y02A40/146
摘要： This invention relates to isolated polynucleotides encoding WUS polypeptides. The invention further provides isolated WUS polypeptides. The invention also provides methods of using the polynucleotides to modulate the level of WUS, improve transformation efficiency, to stimulate plant cell growth, including stem cells, to stimulate organogenesis, to stimulate somatic embryogenesis, to induce apomixis, and to provide a positive selection for cells comprising the polynucleotide. The invention also relates to cells, plants and seeds comprising the polynucleotides of the invention or produced by the methods of the invention.
摘要翻译：本发明涉及编码WUS多肽的分离的多核苷酸。本发明还提供了分离的WUS多肽。本发明还提供了使用多核苷酸来调节WUS水平，提高转化效率，刺激植物细胞生长（包括干细胞）刺激器官发生，刺激体细胞胚发生，诱导无融合生殖以及提供阳性选择的方法包含多核苷酸的细胞。本发明还涉及包含本发明的多核苷酸或通过本发明的方法产生的细胞，植物和种子。

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