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1. 发明申请

US20080047031A1 Novel FRT recombination sites and methods of use 有权
标题翻译：新型FRT重组位点和使用方法
公开(公告)号：US20080047031A1
公开(公告)日：2008-02-21
申请号：US11487300
申请日：2006-07-14
申请人： Yumin Tao , Dennis Bidney , William Gordon-Kamm , Leszek Lyznik
发明人： Yumin Tao , Dennis Bidney , William Gordon-Kamm , Leszek Lyznik
IPC分类号： C12N15/09 , C07H21/00 , C12N5/04 , C40B40/02 , C12N5/10 , C12N1/00
CPC分类号： C12N15/8213 , C12N15/102 , C12N15/1051 , C12N15/1093 , C40B40/08
摘要： Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest, including methods to recombine polynucleotides, assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, and identify and/or characterize transcriptional regulating regions are also provided.
摘要翻译：提供了使用随机修饰的FRT重组位点群的方法和组合来鉴定，分离和/或表征修饰的FRT重组位点。重组生成修饰的FRT重组位点可用于多种感兴趣多核苷酸靶向重组方法，包括重组多核苷酸的方法，评估启动子活性，直接选择转化的生物体，最小化或消除由随机整合到基因组中的表达生物体如植物，去除感兴趣的多核苷酸，组合多个转移盒，反转或切割多核苷酸，以及鉴定和/或表征转录调节区。

2. 发明申请

US20070015195A1 Modified FRT recombination site libraries and methods of use 审中-公开
标题翻译：修改的FRT重组位点文库和使用方法
公开(公告)号：US20070015195A1
公开(公告)日：2007-01-18
申请号：US11487273
申请日：2006-07-14
申请人： Yumin Tao , Dennis Bidney , William Gordon-Kamm , Leszek Lyznik
发明人： Yumin Tao , Dennis Bidney , William Gordon-Kamm , Leszek Lyznik
IPC分类号： C40B30/06 , C40B50/06
CPC分类号： C12N15/8213 , C12N15/102 , C12N15/1051 , C12N15/1093 , C40B40/08
摘要： Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. Kits comprising the library populations of FRT sites are also provided, as are methods to make a library of modified FRT recombination sites. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest.
摘要翻译：提供了使用随机修饰的FRT重组位点群的方法和组合来鉴定，分离和/或表征修饰的FRT重组位点。还提供了包含FRT位点的文库群的试剂盒，以及制备修饰的FRT重组位点的文库的方法。重组生成修饰的FRT重组位点可用于多种目标多重核苷酸重组方法。

3. 发明授权

US5932782A Plant transformation method using agrobacterium species adhered to microprojectiles 失效
标题翻译：植物转化方法使用农杆菌物种附着于微粒
公开(公告)号：US5932782A
公开(公告)日：1999-08-03
申请号：US614403
申请日：1990-11-14
申请人： Dennis Bidney
发明人： Dennis Bidney
IPC分类号： A01H1/00 , C12N5/10 , C12N11/14 , C12N15/09 , C12N15/82 , C12N15/87 , C12R1/91 , A01H1/04
CPC分类号： C12N11/14 , C12N15/8205 , C12N15/87
摘要： Bacteria of the species Agrobacterium are applied to particles which are used in a typical particle gun in a manner which retains their viability after the dry-down process involved in microparticle bombardment. When plant materials are bombarded with particles coated with the bacteria, high rates of stable transformation are achieved.
摘要翻译：将物种农杆菌的细菌施用于在典型的粒子枪中使用的颗粒，其方法是在涉及微粒轰击的干燥过程之后保持其生存力。当用细菌颗粒轰击植物材料时，可以实现高稳定转化率。

4. 发明授权

US09499837B2 Methods and compositions for producing male sterile plants 有权
标题翻译：用于生产雄性不育植物的方法和组合物
公开(公告)号：US09499837B2
公开(公告)日：2016-11-22
申请号：US13526912
申请日：2012-06-19
申请人： Dennis Bidney , Andrew Mark Cigan , Saverio Carl Falco , Huirong Gao , Derek Jantz , Mike Lassner , Keith Lowe , Leszek A Lyznik , James Jefferson Smith
发明人： Dennis Bidney , Andrew Mark Cigan , Saverio Carl Falco , Huirong Gao , Derek Jantz , Mike Lassner , Keith Lowe , Leszek A Lyznik , James Jefferson Smith
IPC分类号： C12N15/82 , C12N15/09
CPC分类号： C12N15/8289 , C12N5/14 , C12N9/22 , C12N15/8213 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C12Q2600/158 , C12Y301/00
摘要： Methods of making a targeted modification in a male fertility gene in the genome of a plant are disclosed. The methods involve contacting a plant cell with an engineered double-strand-break-inducing agent capable of inducing a double-strand break in a target sequence in the male fertility gene and identifying a cell comprising an alteration in the target sequence. Also disclosed are plants, plant cells, plant parts, and seeds comprising a male fertility gene with an alteration in a male fertility gene. Nucleic acid molecules comprising male fertility genes with at least one targeted modification therein, optimized nucleic acid molecules encoding endonucleases that are engineered double-strand-break-inducing agents and expression cassettes, host cells, and plants comprising one or more of the nucleic acid molecules are further disclosed.
摘要翻译：公开了在植物基因组中对雄性能育基因进行靶向修饰的方法。所述方法包括使植物细胞与能够在雄性能育基因中的靶序列中诱导双链断裂的工程化双链断裂诱导剂接触，并鉴定包含靶序列改变的细胞。还公开了包含雄性育性基因改变的雄性育性基因的植物，植物细胞，植物部分和种子。包含其中具有至少一个靶向修饰的雄性能育基因的核酸分子，编码工程化双链断裂诱导剂和表达盒的内切核酸酶的优化核酸分子，宿主细胞和包含一种或多种核酸分子的植物进一步公开。

5. 发明申请

US20060185033A1 Doubled haploid cells, embryos and plants 审中-公开
标题翻译：双倍体单倍体细胞，胚胎和植物
公开(公告)号：US20060185033A1
公开(公告)日：2006-08-17
申请号：US11298973
申请日：2005-12-09
申请人： Zuo-yu Zhao , Dennis Bidney , Evan Elsing , Michael Miller , Xinli Wu , William Gordon-Kamm
发明人： Zuo-yu Zhao , Dennis Bidney , Evan Elsing , Michael Miller , Xinli Wu , William Gordon-Kamm
IPC分类号： A01H1/00 , A01H5/00 , C12N15/82 , C12N5/04
CPC分类号： C12N15/8241 , A01H5/10 , C12N15/8201 , C12N15/829
摘要： Methods for producing homozygous plants, seeds, and plant cells are provided. Methods of forming haploid tissue and then doubling the chromosomes to form doubled haploid cells are provided. Also provided are methods of transformation.
摘要翻译：提供了用于产生纯合植物，种子和植物细胞的方法。提供了形成单倍体组织，然后使染色体加倍以形成双倍体单倍体细胞的方法。还提供了转化的方法。

6. 发明授权

US06486382B1 Use of the green fluorescent protein as a screenable marker for plant transformation 失效
标题翻译：使用绿色荧光蛋白作为植物转化的可筛选标记
公开(公告)号：US06486382B1
公开(公告)日：2002-11-26
申请号：US09214909
申请日：1999-12-20
申请人： William Gordan-Kamm , Dorothy A. Pierce , Benjamin Bowen , Dennis Bidney , Margit Ross , Christopher Scelonge , Michael D. Miller , Gary Sandahl , Lijuan Wang
发明人： William Gordan-Kamm , Dorothy A. Pierce , Benjamin Bowen , Dennis Bidney , Margit Ross , Christopher Scelonge , Michael D. Miller , Gary Sandahl , Lijuan Wang
IPC分类号： C12N1582
CPC分类号： C12N15/8216 , C07K14/43595 , C12N15/8209 , C12N15/8212 , C12N15/8213
摘要： A method for the production of transgenic plants is provided in which a vector carrying a gene encoding the green fluorescent protein is introduced into cells, the cells are screened for the protein and transformed cells are selected and regenerated. The cellular toxicity of the green fluorescent protein is circumvented by regulating expression of the gene encoding the protein or directing the protein to a subcellular compartment where it is not toxic to the cell. DNA constructs are provided for cell transformation in which the expression of a gene encoding the green fluorescent protein is placed under the control of an inducible promoter. In addition, DNA constructs are provided in which a nucleotide sequence encoding the green fluorescent protein is operably linked to a signal sequence which directs the expressed protein to a subcellular compartment where the protein is not toxic to the cell. Oxidative stress to plant cells transformed with GFP also can be ameliorated by transforming cells with an expression vector comprising genes encoding GFP and an oxygen scavenger enzyme such as superoxide dismutase. The toxicity of GFP in transformed plants can be eliminated by excising the screenable marker gene following detection of transformed cells or sectors. The FLP/FRT system is used in conjunction with GFP as a visible marker for transformation and FRT excision. A nucleotide sequence optimized for expression of the green fluorecent protein in plants is also provided.
摘要翻译：提供了一种用于生产转基因植物的方法，其中将携带编码绿色荧光蛋白的基因的载体导入细胞，筛选细胞以进行蛋白质，并选择和再生转化的细胞。绿色荧光蛋白的细胞毒性通过调节编码蛋白质的基因的表达或引导蛋白质转移到对细胞无毒性的亚细胞区域来避免。提供DNA构建体用于细胞转化，其中将编码绿色荧光蛋白的基因的表达置于诱导型启动子的控制之下。此外，提供了DNA构建体，其中编码绿色荧光蛋白的核苷酸序列可操作地连接到将表达的蛋白质导向亚细胞区域的信号序列，其中蛋白质对细胞无毒性。用GFP转化的植物细胞的氧化应激也可以通过用包含编码GFP和氧清除酶如超氧化物歧化酶的基因的表达载体转化细胞来改善。通过在检测到转化的细胞或部分后切除可筛选标记基因，可以消除GFP在转化植物中的毒性。 FLP / FRT系统与GFP结合使用，作为转化和FRT切除的可见标记。还提供了优化用于植物中绿色荧光蛋白表达的核苷酸序列。

7. 发明授权

US5322783A Soybean transformation by microparticle bombardment 失效
标题翻译：大豆转化通过微粒轰击
公开(公告)号：US5322783A
公开(公告)日：1994-06-21
申请号：US422875
申请日：1989-10-17
申请人： Dwight Tomes , Dennis Bidney , Charisse M. Buising
发明人： Dwight Tomes , Dennis Bidney , Charisse M. Buising
IPC分类号： C12N15/82 , C12N15/87 , C12H5/14
CPC分类号： C12N15/8207 , C12N15/8201 , C12N15/87
摘要： Plant cells in tissue culture are more efficiently transformed in a method which involves treatment with a cytokinin followed by incubation for a period sufficient to permit undifferentiated cells in cotyledonary node tissue to differentiate into meristematic cells and to permit the cells to enter the phases between the G1 and division phases of development.
摘要翻译：组织培养中的植物细胞在一种方法中被更有效地转化，所述方法包括用细胞分裂素处理，然后孵育足以允许子叶节点组织中的未分化细胞分化成分生细胞并允许细胞进入G1期和开发阶段。

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