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1. 发明授权

US06921655B1 Endoglucanases and cellulase preparations containing the same 有权
标题翻译：内切葡聚糖酶和含有其的纤维素酶制剂
公开(公告)号：US06921655B1
公开(公告)日：2005-07-26
申请号：US09807933
申请日：1999-10-25
申请人： Yuko Nakamura , Tatsuki Moriya , Yuko Baba , Koji Yanai , Naomi Sumida , Tomoko Nishimura , Kouichirou Murashima , Akitaka Nakane , Takashi Yaguchi , Jinichiro Koga , Takeshi Murakami , Toshiaki Kono
发明人： Yuko Nakamura , Tatsuki Moriya , Yuko Baba , Koji Yanai , Naomi Sumida , Tomoko Nishimura , Kouichirou Murashima , Akitaka Nakane , Takashi Yaguchi , Jinichiro Koga , Takeshi Murakami , Toshiaki Kono
IPC分类号： C11D3/386 , C12N9/42 , C12N15/56 , C12P21/06 , C07H21/04 , C12N9/00 , C12N9/24
CPC分类号： C12N9/2437 , C11D3/38645 , C12Y302/01004
摘要： It is an object of the present invention to provide enzymes that have high endoglucanase activity and yet exhibit high activity even under alkaline conditions, and genes encoding the same. The enzyme according to the invention has the following properties: a) exhibiting endoglucanase activity; and b) capable of completely removing fuzz from regenerated cellulose fabrics at a concentration of 1 mg of the protein/L or below. The enzyme of the invention having endoglucanase activity is a protein comprising the amino acid sequence as shown in SEQ ID NO: 1, 3, 5, 7, 9 or 11; a modified protein thereof exhibiting endoglucanase activity; or a homologue of the protein or the modified protein.
摘要翻译：本发明的目的是提供具有高内切葡聚糖酶活性并且即使在碱性条件下也表现出高活性的酶，以及编码该酶的基因。根据本发明的酶具有以下性质：a）显示内切葡聚糖酶活性; 和b）能够以1mg蛋白质/ L或以下的浓度从再生纤维素织物中完全除去绒毛。具有内切葡聚糖酶活性的本发明的酶是包含如SEQ ID NO：1,3,5,7,9或11所示的氨基酸序列的蛋白质; 其表现出内切葡聚糖酶活性的修饰蛋白质; 或蛋白质或修饰蛋白质的同系物。

2. 发明授权

US07138263B2 Endoglucanase enzyme NCE5 and cellulase preparations containing the same 有权
标题翻译：内切葡聚糖酶NCE5和含有相同的纤维素酶制剂
公开(公告)号：US07138263B2
公开(公告)日：2006-11-21
申请号：US10276779
申请日：2001-05-22
申请人： Koichiro Murashima , Naomi Sumida , Akitaka Nakane , Koji Yanai , Tomoko Nishimura , Jinichiro Koga , Takeshi Murakami , Toshiaki Kono
发明人： Koichiro Murashima , Naomi Sumida , Akitaka Nakane , Koji Yanai , Tomoko Nishimura , Jinichiro Koga , Takeshi Murakami , Toshiaki Kono
IPC分类号： C12N9/42 , C12N15/00 , D21B1/08 , C11D3/00 , C07H21/04
CPC分类号： C12N9/2437 , C11D3/38 , C11D3/38645 , C12Y302/01004 , D06M16/003 , D06P5/02 , D21C5/005 , D21C5/027 , D21C9/002 , Y02W30/648
摘要： There is provided an endoglucanase enzyme, which is useful for reducing fuzz of regenerated cellulose-containing fabrics, improving the touch and appearance, color clarification, localized variation in color, reducing stiffness and using it as components of a detergent, as well as deinking waste paper and improving freeness of paper pulp. A cDNA coding for the endoglucanase enzyme NCE5 was cloned and its DNA sequence and amino acid sequence derived from it were determined.
摘要翻译：提供了内切葡聚糖酶，其可用于减少再生含纤维素织物的绒毛，改善触感和外观，颜色澄清，颜色局部变化，降低刚度并将其用作洗涤剂的组分，以及脱墨废物纸张，提高纸浆的游离度。克隆编码内切葡聚糖酶NCE5的cDNA，并测定其DNA序列和氨基酸序列。

3. 发明申请

US20050143275A1 Endoglucanase nce5 and cellulase preparations containing the same 有权
标题翻译：内切葡聚糖酶nce5和含有相同的纤维素酶制剂
公开(公告)号：US20050143275A1
公开(公告)日：2005-06-30
申请号：US10276779
申请日：2001-05-22
申请人： Koichoro Murashima , Naomi Sumida , Akitaka Nakane , Koji Yanai , Tomoko Nishimura , Jinichiro Koga , Takeshi Murakami , Toshiaki Kono
发明人： Koichoro Murashima , Naomi Sumida , Akitaka Nakane , Koji Yanai , Tomoko Nishimura , Jinichiro Koga , Takeshi Murakami , Toshiaki Kono
IPC分类号： C11D3/38 , C11D3/386 , C12N1/15 , C12N1/19 , C12N9/42 , C12N15/56 , D06M16/00 , D06P5/02 , D21C5/00 , D21C5/02 , D21C9/00 , C07H21/04 , C12N1/16 , C12N1/18
CPC分类号： C12N9/2437 , C11D3/38 , C11D3/38645 , C12Y302/01004 , D06M16/003 , D06P5/02 , D21C5/005 , D21C5/027 , D21C9/002 , Y02W30/648
摘要： There is provided an endoglucanase enzyme, which is useful for reducing fuzz of regenerated cellulose-containing fabrics, improving the touch and appearance, color clarification, localized variation in color, reducing stiffness and using it as components of a detergent, as well as deinking waste paper and improving freeness of paper pulp. A cDNA coding for the endoglucanase enzyme NCE5 was cloned and its DNA sequence and amino acid sequence derived from it were determined.
摘要翻译：提供了内切葡聚糖酶，其可用于减少再生含纤维素织物的绒毛，改善触感和外观，颜色澄清，颜色局部变化，降低刚度并将其用作洗涤剂的组分，以及脱墨废物纸张，提高纸浆的游离度。克隆编码内切葡聚糖酶NCE5的cDNA，并测定其DNA序列和氨基酸序列。

4. 发明授权

US06403362B1 Systems for the mass production of proteins or peptides by microorganisms of the genus humicola 有权
标题翻译：通过腐质霉属微生物大规模生产蛋白质或肽的系统
公开(公告)号：US06403362B1
公开(公告)日：2002-06-11
申请号：US09230225
申请日：1999-03-03
申请人： Tatsuki Moriya , Kouichirou Murashima , Kaoru Aoyagi , Naomi Sumida , Manabu Watanabe , Toru Hamaya , Jinichiro Koga , Toshiaki Kono , Takeshi Murakami
发明人： Tatsuki Moriya , Kouichirou Murashima , Kaoru Aoyagi , Naomi Sumida , Manabu Watanabe , Toru Hamaya , Jinichiro Koga , Toshiaki Kono , Takeshi Murakami
IPC分类号： C12N114
CPC分类号： C12N9/2437 , C12N15/80 , C12Y302/01004 , Y10S530/82 , Y10S530/823
摘要： An expressing system which enables a large amount of production of a protein in Humicola, in particular, Humicola insolens, and particularly host-vector systems and a process for producing a protein using the systems, wherein an expression vector comprising the regulator sequences, i.e., the promoter, the signal sequence, and the terminator, of the cellulase NCE1 gene or NCE2 gene derived from Humicola insolens is constructed and used. The expression vector enables highly efficient production of cellulase NCE4, for example, in Humicola insolens at a rate as high as about 4.5 g or more per one liter of culture.
摘要翻译：一种表达系统，其能够在Humicola中大量生产蛋白质，特别是Humicola insolens，特别是宿主 - 载体系统，以及使用该系统产生蛋白质的方法，其中包含调节序列的表达载体，构建并使用来自Humicola insolens的纤维素酶NCE1基因或NCE2基因的启动子，信号序列和终止子。表达载体能够高效率地生产纤维素酶NCE4，例如在Humicola insolens中以每升培养物高达约4.5g或更高的速率。

5. 发明授权

US6159720A Enzyme endoglucanase and cellulase preparations containing the same 有权
标题翻译：酶内切葡聚糖酶和含有其的纤维素酶制剂
公开(公告)号：US6159720A
公开(公告)日：2000-12-12
申请号：US230222
申请日：1999-03-03
申请人： Kouichirou Murashima , Tatsuki Moriya , Toru Hamaya , Jinichiro Koga , Naomi Sumida , Kaoru Aoyagi , Takeshi Murakami , Toshiaki Kono
发明人： Kouichirou Murashima , Tatsuki Moriya , Toru Hamaya , Jinichiro Koga , Naomi Sumida , Kaoru Aoyagi , Takeshi Murakami , Toshiaki Kono
IPC分类号： C11D3/386 , C12N9/24 , C12N9/42 , C12N15/56 , C12S11/00 , D06M16/00 , D06P5/02 , D06P5/13 , D06P5/15
CPC分类号： C12N9/2437 , C11D3/38645 , C12Y302/01004 , D06M16/003 , D06M2101/06 , D06P5/02 , D06P5/137 , D06P5/158
摘要： A highly active cellulase suitable for use in a removal of nap of cellulose-containing fibers, a process for reducing cellulose-containing fibers and a process for decoloring denim-dyed cellulose-containing fibers, and its gene are provided. A novel cellulase NCE4 isolated from Humicola insolens is a highly active cellulase, and can be used for various treatments of cellulose-containing fibers.
摘要翻译： PCT No.PCT / JP97 / 02561 Sec。 371日期1999年3月3日 102（e）1999年3月3日PCT PCT 1997年7月24日PCT公布。出版物WO98 / 03640 日期1998年1月29日提供了适用于去除含纤维素纤维的绒毛的高活性纤维素酶，还原含纤维素纤维的方法和脱色含有纤维素的纤维素纤维的方法及其基因。从Humicola insolens分离的新型纤维素酶NCE4是一种高度活跃的纤维素酶，可用于纤维素纤维的各种处理。

6. 发明授权

US07432102B2 Transformants producing substance pf1022 derivatives, methods for producing the same, and novel biosynthesis genes 失效
标题翻译：产生物质pf1022衍生物的转化体，其制备方法和新的生物合成基因
公开(公告)号：US07432102B2
公开(公告)日：2008-10-07
申请号：US10472587
申请日：2002-03-22
申请人： Koji Yanai , Naomi Sumida , Manabu Watanabe , Tatsuki Moriya , Takeshi Murakami
发明人： Koji Yanai , Naomi Sumida , Manabu Watanabe , Tatsuki Moriya , Takeshi Murakami
IPC分类号： C12N1/00 , C12P1/00 , C12P17/00 , C12P15/00 , C12P13/00 , C12P7/00 , C12P7/24 , C12P1/02
CPC分类号： C12P17/14 , C12N15/52
摘要： An objective of the present invention is to provide a method for producing substance PF1022 derivatives, in particular PF1022-220 and PF1022-260, by a direct fermentation method, and a transformant to be used for this method. According to the present invention, there is provided a transformant producing substance FF1022 derivatives, which can be obtained by introducing a genes involved in a biosynthetic pathway from chorismic acid to p-aminophenylpyruvic acid, including a papA gene encoding 4-amino-4-deoxychorismate synthase. which gene comprises the DNA sequence encoding the amino acid sequence of SEQ ID NO: 2; a papB gene encoding 4-amino-4-deoxyclaismate mutase, which gene comprises the DNA sequence encoding the amino acid sequence of SEQ ID NO: 4; and a papC gene encoding 4-amino-4-deoxyprepbenate dehydrogenase, which gene comprises the DNA sequence encoding the amino acid sequence of SEQ ID NO: 6, into a phenylalanine auxotrophic host induced from an organism that produces a substance PF1022. According to the present invention, there is also provided a process of producing substance PF1022 derivative, comprising steps of culturing the above-mentioned transformant and collecting the substance PF1022 derivatives.
摘要翻译：本发明的目的是提供通过直接发酵方法生产物质PF1022衍生物，特别是PF1022-220和PF1022-260的方法，以及用于该方法的转化体。根据本发明，提供了转化体生产物质FF1022衍生物，其可以通过将参与生物合成途径的基因从氯磺酸引入对氨基苯基丙酮酸而获得，包括编码4-氨基-4-脱氧胸苷酸的papA基因合酶。该基因包含编码SEQ ID NO：2的氨基酸序列的DNA序列; 编码4-氨基-4-脱氧胆酸酯变位酶的papB基因，该基因包含编码SEQ ID NO：4的氨基酸序列的DNA序列; 和编码4-氨基-4-脱氧降磷酸脱氢酶的papC基因，该基因包含编码SEQ ID NO：6的氨基酸序列的DNA序列，由产生PF1022的生物体诱导的苯丙氨酸营养缺陷型宿主中。根据本发明，还提供了生产物质PF1022衍生物的方法，包括培养上述转化体并收集物质PF1022衍生物的步骤。

7. 发明授权

US06277596B1 Regulatory sequence of cellulase cbh1 genes originating in trichoderma viride and system for mass-producing proteins or peptides therewith 有权
标题翻译：来源于绿叶病毒的纤维素酶cbh1基因的调控序列及其大量生产蛋白质或肽的系统
公开(公告)号：US06277596B1
公开(公告)日：2001-08-21
申请号：US09254733
申请日：1999-05-07
申请人： Manabu Watanabe , Tatsuki Moriya , Kaoru Aoyagi , Naomi Sumida , Takeshi Murakami
发明人： Manabu Watanabe , Tatsuki Moriya , Kaoru Aoyagi , Naomi Sumida , Takeshi Murakami
IPC分类号： C12P2106
CPC分类号： C12Y302/01091 , C12N9/2437 , C12N9/2482 , C12N15/80 , C12Y302/01004 , C12Y302/01008
摘要： A high-yield production system for proteins and peptides has been established, and especially a high-yield production system for cellulase in Trichoderma viride and similar filamentous fungi. The cellulase cbh1 gene regulator sequence derived from Trichoderma viride gives high expression of target proteins. The regulator sequence can therefore be used for high-yield expression of target proteins, especially cellulase. In particular, 15 g/L of an endoglucanase derived from Humicola insolens was successfully produced with this regulator sequence.
摘要翻译：已经建立了高产量的蛋白质和肽的生产系统，特别是在绿色木霉和类似丝状真菌中的纤维素酶的高产量生产系统。衍生自绿色木霉的纤维素酶cbh1基因调控序列提供了靶蛋白的高表达。因此，调节序列可用于靶蛋白，特别是纤维素酶的高产量表达。特别地，用该调节剂序列成功地产生了15g / L源自Humicola insolens的内切葡聚糖酶。

8. 发明授权

US08440400B2 Process for amplifying DNA in cells 有权
标题翻译：在细胞中扩增DNA的方法
公开(公告)号：US08440400B2
公开(公告)日：2013-05-14
申请号：US12996108
申请日：2009-06-05
申请人： Takeshi Murakami , Naomi Sumida , Koji Yanai
发明人： Takeshi Murakami , Naomi Sumida , Koji Yanai
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , C12N15/63 , C12Q1/6844 , C12Q2545/101 , C12Q2531/10
摘要： The present invention relates to a process for efficiently amplifying a giant DNA. More particularly, the present invention relates to a process for amplifying DNA in a cell, comprising amplifying the DNA as the target of amplification in the presence of DNAs selected from the following (i), (ii) and (iii): (i) DNA encoding a protein selected from the following 1), 2) and 3): 1) a protein consisting of the amino acid sequence of SEQ ID NO: 1, 2) a protein comprising an amino acid sequence which has a deletion, substitution, insertion or addition of one or more amino acids in the amino acid sequence of SEQ ID NO: 1, and 3) a protein comprising an amino acid sequence which has an identity of 90% or more to the amino acid sequence of SEQ ID NO: 1, (ii) DNA consisting of the nucleotide sequence of SEQ ID NO: 2, and (iii) DNA hybridizing to the nucleotide sequence of SEQ ID NO: 2 under stringent conditions.
摘要翻译：本发明涉及一种有效扩增巨DNA的方法。更具体地说，本发明涉及一种用于扩增细胞中DNA的方法，包括在选自以下（i），（ii）和（iii）的DNA存在下扩增作为扩增靶的DNA：（i）编码选自以下1），2）和3）的蛋白质的DNA：1）由SEQ ID NO：1的氨基酸序列组成的蛋白质，2）包含氨基酸序列的蛋白质，所述氨基酸序列具有缺失，取代，在SEQ ID NO：1的氨基酸序列中插入或添加一个或多个氨基酸，以及3）包含与SEQ ID NO：1的氨基酸序列具有90％以上相同性的氨基酸序列的蛋白质。 1，（ii）由SEQ ID NO：2的核苷酸序列组成的DNA，和（iii）在严格条件下与SEQ ID NO：2的核苷酸序列杂交的DNA。

9. 发明申请

US20110171692A1 PROCESS FOR AMPLIFYING DNA IN CELLS 有权
标题翻译：在细胞中放大DNA的方法
公开(公告)号：US20110171692A1
公开(公告)日：2011-07-14
申请号：US12996108
申请日：2009-06-05
申请人： Takeshi Murakami , Naomi Sumida , Koji Yanai
发明人： Takeshi Murakami , Naomi Sumida , Koji Yanai
IPC分类号： C12P19/34 , C12N1/21 , C12N1/00 , C07K14/36 , C12N15/63
CPC分类号： C12Q1/686 , C12N15/63 , C12Q1/6844 , C12Q2545/101 , C12Q2531/10
摘要： The present invention relates to a process for efficiently amplifying a giant DNA. More particularly, the present invention relates to a process for amplifying DNA in a cell, comprising amplifying the DNA as the target of amplification in the presence of DNAs selected from the following (i), (ii) and (iii): (i) DNA encoding a protein selected from the following 1), 2) and 3): 1) a protein consisting of the amino acid sequence of SEQ ID NO: 1, 2) a protein comprising an amino acid sequence which has a deletion, substitution, insertion or addition of one or more amino acids in the amino acid sequence of SEQ ID NO: 1, and 3) a protein comprising an amino acid sequence which has an identity of 90% or more to the amino acid sequence of SEQ ID NO: 1, (ii) DNA consisting of the nucleotide sequence of SEQ ID NO: 2, and (iii) DNA hybridizing to the nucleotide sequence of SEQ ID NO: 2 under stringent conditions.
摘要翻译：本发明涉及一种有效扩增巨DNA的方法。更具体地说，本发明涉及一种用于扩增细胞中DNA的方法，包括在选自以下（i），（ii）和（iii）的DNA存在下扩增作为扩增靶的DNA：（i）编码选自以下1），2）和3）的蛋白质的DNA：1）由SEQ ID NO：1的氨基酸序列组成的蛋白质，2）包含氨基酸序列的蛋白质，所述氨基酸序列具有缺失，取代，在SEQ ID NO：1的氨基酸序列中插入或添加一个或多个氨基酸，以及3）包含与SEQ ID NO：1的氨基酸序列具有90％以上相同性的氨基酸序列的蛋白质。 1，（ii）由SEQ ID NO：2的核苷酸序列组成的DNA，和（iii）在严格条件下与SEQ ID NO：2的核苷酸序列杂交的DNA。

10. 发明授权

US6127160A Protein having cellulase activities and process for producing the same 有权
标题翻译：具有纤维素酶活性的蛋白质及其制备方法
公开(公告)号：US6127160A
公开(公告)日：2000-10-03
申请号：US142759
申请日：1998-09-14
申请人： Takashi Yamanobe , Manabu Watanabe , Toru Hamaya , Naomi Sumida , Kaoru Aoyagi , Takeshi Murakami
发明人： Takashi Yamanobe , Manabu Watanabe , Toru Hamaya , Naomi Sumida , Kaoru Aoyagi , Takeshi Murakami
IPC分类号： C12N9/42 , C12N15/56
CPC分类号： C12N9/2437
摘要： The object of the present invention is to analyze the amino acid sequence of the protein constituting the cellulase system, to clone the gene coding for the components of the cellulase system, and to establish a technique of inserting a clonal gene into the above strain thereby to produce cellulase having enhanced avicellase activity. The present invention relates to a protein having a part or the whole of the amino acid sequence depicted in the sequence listing under SEQ ID NO:1 and possessing cellulase activity, a DNA coding for the protein, an expression vector containing the DNA, a microorganism as transformed by the expression vector, and a process for producing a protein having enhanced cellulase activity by using the microorganism.
摘要翻译： PCT No.PCT / JP97 / 00824 Sec。 371日期：1998年9月14日 102（e）1998年9月14日PCT PCT 1997年3月14日PCT公布。出版物WO97 / 33982 日本1997年9月18日本发明的目的是分析构成纤维素酶体系的蛋白质的氨基酸序列，克隆编码纤维素酶系统成分的基因，并建立将克隆基因插入由此产生具有增强的avicellase活性的纤维素酶。本发明涉及具有SEQ ID NO：1所示的序列表中具有纤维素酶活性的部分或全部氨基酸序列的蛋白质，编码该蛋白质的DNA，含有该DNA的表达载体，微生物通过表达载体转化，以及通过使用微生物生产具有增强的纤维素酶活性的蛋白质的方法。

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