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1. 发明授权

US06921655B1 Endoglucanases and cellulase preparations containing the same 有权
标题翻译：内切葡聚糖酶和含有其的纤维素酶制剂
公开(公告)号：US06921655B1
公开(公告)日：2005-07-26
申请号：US09807933
申请日：1999-10-25
申请人： Yuko Nakamura , Tatsuki Moriya , Yuko Baba , Koji Yanai , Naomi Sumida , Tomoko Nishimura , Kouichirou Murashima , Akitaka Nakane , Takashi Yaguchi , Jinichiro Koga , Takeshi Murakami , Toshiaki Kono
发明人： Yuko Nakamura , Tatsuki Moriya , Yuko Baba , Koji Yanai , Naomi Sumida , Tomoko Nishimura , Kouichirou Murashima , Akitaka Nakane , Takashi Yaguchi , Jinichiro Koga , Takeshi Murakami , Toshiaki Kono
IPC分类号： C11D3/386 , C12N9/42 , C12N15/56 , C12P21/06 , C07H21/04 , C12N9/00 , C12N9/24
CPC分类号： C12N9/2437 , C11D3/38645 , C12Y302/01004
摘要： It is an object of the present invention to provide enzymes that have high endoglucanase activity and yet exhibit high activity even under alkaline conditions, and genes encoding the same. The enzyme according to the invention has the following properties: a) exhibiting endoglucanase activity; and b) capable of completely removing fuzz from regenerated cellulose fabrics at a concentration of 1 mg of the protein/L or below. The enzyme of the invention having endoglucanase activity is a protein comprising the amino acid sequence as shown in SEQ ID NO: 1, 3, 5, 7, 9 or 11; a modified protein thereof exhibiting endoglucanase activity; or a homologue of the protein or the modified protein.
摘要翻译：本发明的目的是提供具有高内切葡聚糖酶活性并且即使在碱性条件下也表现出高活性的酶，以及编码该酶的基因。根据本发明的酶具有以下性质：a）显示内切葡聚糖酶活性; 和b）能够以1mg蛋白质/ L或以下的浓度从再生纤维素织物中完全除去绒毛。具有内切葡聚糖酶活性的本发明的酶是包含如SEQ ID NO：1,3,5,7,9或11所示的氨基酸序列的蛋白质; 其表现出内切葡聚糖酶活性的修饰蛋白质; 或蛋白质或修饰蛋白质的同系物。

2. 发明授权

US07790425B2 Saponin-decomposing enzyme, gene thereof and large-scale production system for producing soyasapogenol B 失效
公开(公告)号：US07790425B2
公开(公告)日：2010-09-07
申请号：US12591586
申请日：2009-11-24
申请人： Manabu Watanabe , Naoki Mido , Takayoshi Tamura , Naomi Sumida , Takashi Yaguchi
发明人： Manabu Watanabe , Naoki Mido , Takayoshi Tamura , Naomi Sumida , Takashi Yaguchi
IPC分类号： C12N9/24 , C12N15/00 , C12P21/06
CPC分类号： C12N9/2402 , C12N15/8243 , C12P33/00
摘要： The present invention provides a protein having saponin-decomposing activity, more specifically a protein which can decompose a glycoside having soyasapogenol B as an aglycone to produce soyasapogenol B, a polynucleotide encoding such a protein, and a method of producing soyasapogenol B on a large scale using the same. A protein according to the present invention are concerned with (a), (b) or (c), namely (a) a protein comprising an amino acid sequence selected from the group consisting of the amino acid sequences shown in SEQ ID NOs: 2, 4, and 6; (b) a protein that has at least 50% homology to the protein comprising the amino acid sequence of the sequence described in (a) and having saponin-decomposing activity; or (c) a protein comprising a modified amino acid sequence of the sequence described in (a) that has one or more amino acid residues deleted, substituted, inserted, or added and having saponin-decomposing activity.

3. 发明申请

US20080187972A1 Saponin-decomposing enzyme, gene thereof and large-scale production system for producing soyasapogenol B 失效
公开(公告)号：US20080187972A1
公开(公告)日：2008-08-07
申请号：US12010055
申请日：2008-01-18
申请人： Manabu Watanabe , Naoki Mido , Takayoshi Tamura , Naomi Sumida , Takashi Yaguchi
发明人： Manabu Watanabe , Naoki Mido , Takayoshi Tamura , Naomi Sumida , Takashi Yaguchi
IPC分类号： C12P7/02 , C12N9/00
CPC分类号： C12N9/2402 , C12N15/8243 , C12P33/00
摘要： The present invention provides a protein having saponin-decomposing activity, more specifically a protein which can decompose a glycoside having soyasapogenol B as an aglycone to produce soyasapogenol B, a polynucleotide encoding such a protein, and a method of producing soyasapogenol B on a large scale using the same. A protein according to the present invention are concerned with (a), (b) or (c), namely (a) a protein comprising an amino acid sequence selected from the group consisting of the amino acid sequences shown in SEQ ID NOs: 2, 4, and 6; (b) a protein that has at least 50% homology to the protein comprising the amino acid sequence of the sequence described in (a) and having saponin-decomposing activity; or (c) a protein comprising a modified amino acid sequence of the sequence described in (a) that has one or more amino acid residues deleted, substituted, inserted, or added and having saponin-decomposing activity.

4. 发明申请

US20060275862A1 Saponin-decomposing enzyme, gene thereof and large-scale production system for producing soyasapogenol B 失效
公开(公告)号：US20060275862A1
公开(公告)日：2006-12-07
申请号：US11504646
申请日：2006-08-16
申请人： Manabu Watanabe , Naoki Mido , Takayoshi Tamura , Naomi Sumida , Takashi Yaguchi
发明人： Manabu Watanabe , Naoki Mido , Takayoshi Tamura , Naomi Sumida , Takashi Yaguchi
IPC分类号： C12P33/00 , C12P21/06 , C07H21/04 , C12N9/24 , C12N15/74 , C12N1/16
CPC分类号： C12N9/2402 , C12N15/8243 , C12P33/00
摘要： The present invention provides a protein having saponin-decomposing activity, more specifically a protein which can decompose a glycoside having soyasapogenol B as an aglycone to produce soyasapogenol B, a polynucleotide encoding such a protein, and a method of producing soyasapogenol B on a large scale using the same. A protein according to the present invention are concerned with (a), (b) or (c), namely (a) a protein comprising an amino acid sequence selected from the group consisting of the amino acid sequences shown in SEQ ID NOs: 2, 4, and 6; (b) a protein that has at least 50% homology to the protein comprising the amino acid sequence of the sequence described in (a) and having saponin-decomposing activity; or (c) a protein comprising a modified amino acid sequence of the sequence described in (a) that has one or more amino acid residues deleted, substituted, inserted, or added and having saponin-decomposing activity.

5. 发明授权

US07022508B2 Saponin-digesting enzymes, genes thereof and soyasapogenol B mass production system 失效
标题翻译：皂苷消化酶，其基因和大豆皂甙B大量生产系统
公开(公告)号：US07022508B2
公开(公告)日：2006-04-04
申请号：US10479787
申请日：2002-06-06
申请人： Manabu Watanabe , Naoki Mido , Takayoshi Tamura , Naomi Sumida , Takashi Yaguchi
发明人： Manabu Watanabe , Naoki Mido , Takayoshi Tamura , Naomi Sumida , Takashi Yaguchi
IPC分类号： C12Q1/68 , C07H21/04 , C12N9/24 , C12P33/00 , C12R1/885
CPC分类号： C12N9/2402 , C12N15/8243 , C12P33/00
摘要： The present invention provides a protein having saponin-decomposing activity, more specifically a protein which can decompose a glycoside having soyasapogenol B as an aglycone to produce soyasapogenol B, a polynucleotide encoding such a protein, and a method of producing soyasapogenol B on a large scale using the same. A protein according to the present invention are concerned with (a), (b) or (c), namely (a) a protein comprising an amino acid sequence selected from the group consisting of the amino acid sequences shown in SEQ ID NOs: 2, 4, and 6; (b) a protein that has at least 50% homology to the protein comprising the amino acid sequence of the sequence described in (a) and having saponin-decomposing activity; or (c) a protein comprising a modified amino acid sequence of the sequence described in (a) that has one or more amino acid residues deleted, substituted, inserted, or added and having saponin-decomposing activity.
摘要翻译：本发明提供具有皂苷分解活性的蛋白质，更具体地说，可以分解具有大豆皂甙元酚B作为糖苷配基的糖苷以制备大豆皂甙元B的蛋白质，编码这种蛋白质的多核苷酸以及大规模生产大豆皂甙元B的方法的蛋白质使用相同根据本发明的蛋白质涉及（a），（b）或（c），即（a）包含选自SEQ ID NO：2所示的氨基酸序列的氨基酸序列的蛋白质，4和6; （b）与包含（a）中描述的序列的氨基酸序列的蛋白质具有至少50％同源性且具有皂苷分解活性的蛋白质; 或（c）包含（a）中描述的具有一个或多个氨基酸残基被缺失，取代，插入或添加并具有皂角苷分解活性的修饰的氨基酸序列的蛋白质。

6. 发明申请

US20100167362A1 Saponin-decomposing enzyme, gene thereof and large-scale production system for producing soyasapogenol B 失效
标题翻译：皂苷分解酶，其基因和大规模生产大豆皂甙B的生产体系
公开(公告)号：US20100167362A1
公开(公告)日：2010-07-01
申请号：US12591586
申请日：2009-11-24
申请人： Manabu Watanabe , Naoki Mido , Takayoshi Tamura , Naomi Sumida , Takashi Yaguchi
发明人： Manabu Watanabe , Naoki Mido , Takayoshi Tamura , Naomi Sumida , Takashi Yaguchi
IPC分类号： C12P7/02
CPC分类号： C12N9/2402 , C12N15/8243 , C12P33/00
摘要： The present invention provides a protein having saponin-decomposing activity, more specifically a protein which can decompose a glycoside having soyasapogenol B as an aglycone to produce soyasapogenol B, a polynucleotide encoding such a protein, and a method of producing soyasapogenol B on a large scale using the same. A protein according to the present invention are concerned with (a), (b) or (c), namely (a) a protein comprising an amino acid sequence selected from the group consisting of the amino acid sequences shown in SEQ ID NOs: 2, 4, and 6; (b) a protein that has at least 50% homology to the protein comprising the amino acid sequence of the sequence described in (a) and having saponin-decomposing activity; or (c) a protein comprising a modified amino acid sequence of the sequence described in (a) that has one or more amino acid residues deleted, substituted, inserted, or added and having saponin-decomposing activity.
摘要翻译：本发明提供具有皂苷分解活性的蛋白质，更具体地说，可以分解具有大豆皂甙元酚B作为糖苷配基的糖苷以制备大豆皂甙元B的蛋白质，编码这种蛋白质的多核苷酸以及大规模生产大豆皂甙元B的方法的蛋白质使用相同根据本发明的蛋白质涉及（a），（b）或（c），即（a）包含选自SEQ ID NO：2所示的氨基酸序列的氨基酸序列的蛋白质，4和6; （b）与包含（a）中所述序列的氨基酸序列并且具有皂苷分解活性的蛋白质具有至少50％同源性的蛋白质; 或（c）包含（a）中描述的具有一个或多个氨基酸残基被缺失，取代，插入或添加并具有皂苷分解活性的修饰的氨基酸序列的蛋白质。

7. 发明授权

US07670803B2 Saponin-decomposing enzyme, gene thereof and large-scale production sytem for producing soyasapogenol B 失效
标题翻译：皂苷分解酶，其基因和大规模生产大豆皂甙B的生产系统
公开(公告)号：US07670803B2
公开(公告)日：2010-03-02
申请号：US12385293
申请日：2009-04-03
申请人： Manabu Watanabe , Naoki Mido , Takayoshi Tamura , Naomi Sumida , Takashi Yaguchi
发明人： Manabu Watanabe , Naoki Mido , Takayoshi Tamura , Naomi Sumida , Takashi Yaguchi
IPC分类号： C07H21/04 , C12N9/24 , C12Q1/68
CPC分类号： C12N9/2402 , C12N15/8243 , C12P33/00
摘要： The present invention provides a protein having saponin-decomposing activity, more specifically a protein which can decompose a glycoside having soyasapogenol B as an aglycone to produce soyasapogenol B, a polynucleotide encoding such a protein, and a method of producing soyasapogenol B on a large scale using the same. A protein according to the present invention are concerned with (a), (b) or (c), namely (a) a protein comprising an amino acid sequence selected from the group consisting of the amino acid sequences shown in SEQ ID NOs: 2, 4, and 6; (b) a protein that has at least 50% homology to the protein comprising the amino acid sequence of the sequence described in (a) and having saponin-decomposing activity; or (c) a protein comprising a modified amino acid sequence of the sequence described in (a) that has one or more amino acid residues deleted, substituted, inserted, or added and having saponin-decomposing activity.
摘要翻译：本发明提供具有皂苷分解活性的蛋白质，更具体地说，可以分解具有大豆皂甙元酚B作为糖苷配基的糖苷以制备大豆皂甙元B的蛋白质，编码这种蛋白质的多核苷酸以及大规模生产大豆皂甙元B的方法的蛋白质使用相同根据本发明的蛋白质涉及（a），（b）或（c），即（a）包含选自SEQ ID NO：2所示的氨基酸序列的氨基酸序列的蛋白质，4和6; （b）与包含（a）中所述序列的氨基酸序列并且具有皂苷分解活性的蛋白质具有至少50％同源性的蛋白质; 或（c）包含（a）中描述的具有一个或多个氨基酸残基被缺失，取代，插入或添加并具有皂苷分解活性的修饰的氨基酸序列的蛋白质。

8. 发明申请

US20060115886A1 Saponin-decomposing enzyme, gene thereof and large-scale production system for producing soyasapogenol B 失效
公开(公告)号：US20060115886A1
公开(公告)日：2006-06-01
申请号：US11320412
申请日：2005-12-29
申请人： Manabu Watanabe , Naoki Mido , Takayoshi Tamura , Naomi Sumida , Takashi Yaguchi
发明人： Manabu Watanabe , Naoki Mido , Takayoshi Tamura , Naomi Sumida , Takashi Yaguchi
IPC分类号： C12N9/00 , C07H21/04 , C12P21/06 , C12N9/24
CPC分类号： C12N9/2402 , C12N15/8243 , C12P33/00
摘要： The present invention provides a protein having saponin-decomposing activity, more specifically a protein which can decompose a glycoside having soyasapogenol B as an aglycone to produce soyasapogenol B, a polynucleotide encoding such a protein, and a method of producing soyasapogenol B on a large scale using the same. A protein according to the present invention are concerned with (a), (b) or (c), namely (a) a protein comprising an amino acid sequence selected from the group consisting of the amino acid sequences shown in SEQ ID NOs: 2, 4, and 6; (b) a protein that has at least 50% homology to the protein comprising the amino acid sequence of the sequence described in (a) and having saponin-decomposing activity; or (c) a protein comprising a modified amino acid sequence of the sequence described in (a) that has one or more amino acid residues deleted, substituted, inserted, or added and having saponin-decomposing activity.

9. 发明申请

US20090226967A1 Saponin-decomposing enzyme, gene thereof and large-scale production system for producing soyasapogenol B 失效
标题翻译：皂苷分解酶，其基因和大规模生产大豆皂甙B的生产体系
公开(公告)号：US20090226967A1
公开(公告)日：2009-09-10
申请号：US12385293
申请日：2009-04-03
申请人： Manabu Watanabe , Naoki Mido , Takayoshi Tamura , Naomi Sumida , Takashi Yaguchi
发明人： Manabu Watanabe , Naoki Mido , Takayoshi Tamura , Naomi Sumida , Takashi Yaguchi
IPC分类号： C12P21/06 , C07H21/04 , C12N15/63 , C12N1/15
CPC分类号： C12N9/2402 , C12N15/8243 , C12P33/00
摘要： The present invention provides a protein having saponin-decomposing activity, more specifically a protein which can decompose a glycoside having soyasapogenol B as an aglycone to produce soyasapogenol B, a polynucleotide encoding such a protein, and a method of producing soyasapogenol B on a large scale using the same. A protein according to the present invention are concerned with (a), (b) or (c), namely (a) a protein comprising an amino acid sequence selected from the group consisting of the amino acid sequences shown in SEQ ID NOs: 2, 4, and 6; (b) a protein that has at least 50% homology to the protein comprising the amino acid sequence of the sequence described in (a) and having saponin-decomposing activity; or (c) a protein comprising a modified amino acid sequence of the sequence described in (a) that has one or more amino acid residues deleted, substituted, inserted, or added and having saponin-decomposing activity.
摘要翻译：本发明提供具有皂苷分解活性的蛋白质，更具体地说，可以分解具有大豆皂甙元酚B作为糖苷配基的糖苷以制备大豆皂甙元B的蛋白质，编码这种蛋白质的多核苷酸以及大规模生产大豆皂甙元B的方法的蛋白质使用相同根据本发明的蛋白质涉及（a），（b）或（c），即（a）包含选自SEQ ID NO：2所示的氨基酸序列的氨基酸序列的蛋白质，4和6; （b）与包含（a）中描述的序列的氨基酸序列的蛋白质具有至少50％同源性且具有皂苷分解活性的蛋白质; 或（c）包含（a）中描述的具有一个或多个氨基酸残基被缺失，取代，插入或添加并具有皂角苷分解活性的修饰的氨基酸序列的蛋白质。

10. 发明授权

US07335498B2 Saponin-decomposing enzyme, gene thereof and large-scale production system for producing soyasapogenol B 失效
标题翻译：皂苷分解酶，其基因和大规模生产大豆皂甙B的生产体系
公开(公告)号：US07335498B2
公开(公告)日：2008-02-26
申请号：US11504646
申请日：2006-08-16
申请人： Manabu Watanabe , Naoki Mido , Takayoshi Tamura , Naomi Sumida , Takashi Yaguchi
发明人： Manabu Watanabe , Naoki Mido , Takayoshi Tamura , Naomi Sumida , Takashi Yaguchi
IPC分类号： C12N9/24 , C12N15/00 , C12P21/06
CPC分类号： C12N9/2402 , C12N15/8243 , C12P33/00
摘要： The present invention provides a protein having saponin-decomposing activity, more specifically a protein which can decompose a glycoside having soyasapogenol B as an aglycone to produce soyasapogenol B, a polynucleotide encoding such a protein, and a method of producing soyasapogenol B on a large scale using the same. A protein according to the present invention are concerned with (a), (b) or (c), namely (a) a protein comprising an amino acid sequence selected from the group consisting of the amino acid sequences shown in SEQ ID NOs: 2, 4, and 6; (b) a protein that has at least 50% homology to the protein comprising the amino acid sequence of the sequence described in (a) and having saponin-decomposing activity; or (c) a protein comprising a modified amino acid sequence of the sequence described in (a) that has one or more amino acid residues deleted, substituted, inserted, or added and having saponin-decomposing activity.
摘要翻译：本发明提供具有皂苷分解活性的蛋白质，更具体地说，可以分解具有大豆皂甙元酚B作为糖苷配基的糖苷以制备大豆皂甙元B的蛋白质，编码这种蛋白质的多核苷酸以及大规模生产大豆皂甙元B的方法的蛋白质使用相同根据本发明的蛋白质涉及（a），（b）或（c），即（a）包含选自SEQ ID NO：2所示的氨基酸序列的氨基酸序列的蛋白质，4和6; （b）与包含（a）中描述的序列的氨基酸序列的蛋白质具有至少50％同源性且具有皂苷分解活性的蛋白质; 或（c）包含（a）中描述的具有一个或多个氨基酸残基被缺失，取代，插入或添加并具有皂角苷分解活性的修饰的氨基酸序列的蛋白质。

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