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1. 发明申请

US20060037100A1 Fungal resistant transgenic pepper plants and their production method 审中-公开
标题翻译：真菌抗性转基因胡椒植物及其生产方法
公开(公告)号：US20060037100A1
公开(公告)日：2006-02-16
申请号：US10916419
申请日：2004-08-12
申请人： Young Kim , Moon Ko , Hyo Seo , Jung Cho , Pill-Soon Song
发明人： Young Kim , Moon Ko , Hyo Seo , Jung Cho , Pill-Soon Song
IPC分类号： C12N15/82 , A01H1/00 , C12N15/87
CPC分类号： C12N9/16 , C07K14/415 , C12N15/8282
摘要： A stable pepper transformation was established using Agrobacterium mediated method. Pepper plants were transformed with PepEST or PepDef gene, where the expression of the nucleic acid sequence in the plant resulted in increased resistance to fungal infection as compared to the wild type plant. Provided are agricultural products including seeds produced by the transgenic plants. Also provided are vectors and host cells containing the nucleic acids coding PepEST and PepDef, respectively.
摘要翻译：使用农杆菌介导的方法建立稳定的胡椒转化。用PepEST或PepDef基因转化辣椒植物，与野生型植物相比，植物中核酸序列的表达导致对真菌感染的抗性增加。提供农产品，包括由转基因植物生产的种子。还提供了分别含有编码PepEST和PepDef的核酸的载体和宿主细胞。

2. 发明申请

US20050260184A1 DUAL ROLES OF PEPPER ESTERASE AS A BIOCONTROL AGENT 失效
标题翻译：作为生物处理剂的脯氨酸酯的双重作用
公开(公告)号：US20050260184A1
公开(公告)日：2005-11-24
申请号：US10848375
申请日：2004-05-19
申请人： Young Kim , Hyo Seo , Moon Ko , Boung-Jun Oh , Pill-Soon Song
发明人： Young Kim , Hyo Seo , Moon Ko , Boung-Jun Oh , Pill-Soon Song
IPC分类号： A01N37/18 , A01N37/46 , A61K38/46 , C12N9/16 , C12N9/18 , C12N15/82
CPC分类号： C12Y301/01 , A01N37/18 , A01N37/46 , A01N65/08 , A61K38/00 , C12N9/18 , C12N15/8282
摘要： This invention relates to dual roles of the pepper esterase protein as a biocontrol agent that can elicit defense reactions in plant cells and as direct fungal inhibitor that can block fungal penetration into plant cells. The possible enzymatic actions of the PepEST protein were elucidated in both fungus and plant to gain insight into the molecular mechanisms involved in the fungal resistance. Exogenously treated PepEST protein on the unripe pepper fruits decomposed the cuticles of the fruits and released glycerol and 9-octadecenamide. The treatment resulted in the massive generation of hydrogen peroxide in the unripe pepper fruit and simultaneously elicited the expression of defense-related genes in both absence and presence of the fungus.
摘要翻译：本发明涉及胡椒酯酶蛋白作为可在植物细胞中引发防御反应的生物防治剂的双重作用，以及可阻断真菌渗入植物细胞的直接真菌抑制剂。在真菌和植物中阐明了PepEST蛋白的可能的酶作用，以了解参与真菌抗性的分子机制。未成熟胡椒果实外源性处理的PepEST蛋白分解果实的角质层，释放甘油和9-十八碳烯酰胺。该处理导致未成熟胡椒果实中大量生成过氧化氢，并且在真菌存在和存在的同时引发防御相关基因的表达。

3. 发明授权

US07285652B2 Isolated nucleic acid molecule encoding the modified phytochrome A 失效
标题翻译：分离的编码改性植物色素A的核酸分子
公开(公告)号：US07285652B2
公开(公告)日：2007-10-23
申请号：US11129459
申请日：2005-05-16
申请人： Jeong-Il Kim , Yun-Jeong Han , Pill-Soon Song
发明人： Jeong-Il Kim , Yun-Jeong Han , Pill-Soon Song
IPC分类号： C07H21/04
CPC分类号： C12N15/8261 , C07K14/415 , Y02A40/146
摘要： The present invention includes modified phytochrome A (PHYA) nucleic acid molecules in which Pr absorption spectra have been shifted to longer wavelength (i.e. bathochromism or red-shift). The plants with the bathochromic phytochromes are expected to respond to canopy and shade conditions for growth and development with greater efficiency than the plants with wild-type phytochrome (i.e. suppression of shade avoidance reactions in plants). Since the shade avoidance reactions in plants induce a rapid and dramatic increase in the extension growth of stems and petioles at the expense of leaf growth, storage organ production, and reproductive development, it causes significant losses of crop yields. Thus, the bathochromic phytochromes that utilize the shade light efficiently would suppress the shade avoidance reactions in plants, giving plants the tolerance to shade. In this invention, several bathochromic phytochromes were generated by site-directed mutagenesis in the region of bilin lyase domain in plant PHYA, and their ability to suppress the shade avoidance reactions were examined by transforming the bathochromic phytochromes into PHYA deficient Arabidopsis thaliana (ecotype col-0). The transgenic plants with the bathochromic phytochromes showed significantly increased shade tolerance compared to wild-type plants and transgenic plants with wild-type phytochromes. Therefore, the present invention can be utilized to suppress plants' shade avoidance that is one of major causes to induce crop-yield losses, and ultimately to generate shade tolerant plants with higher yields. The invention also includes plants having at least one cell expressing the modified PHYA, vectors comprising at least one portion of the modified PHYA nucleic acids, and methods using such vectors for producing plants with shade tolerance.
摘要翻译：本发明包括修饰的植物色素A（PHYA）核酸分子，其中Pr吸收光谱已经转移到更长的波长（即，红移或红移）。具有红色色素植物色素的植物预期对具有野生型植物色素的植物（即抑制植物中的树荫回避反应）的效率比用于生长和发育的冠层和阴凉条件更有效。由于植物中的避光反应以叶片生长，储存器官生产和繁殖发育为代价诱导茎和叶柄的延伸生长快速和显着地增加，导致作物产量的显着损失。因此，有效利用遮光效果的红色植物色素可以抑制植物的避光反应，使植物具有遮荫性。在本发明中，通过在植物PHYA中的胆固醇裂解酶结构域中的定点诱变产生了几种红色植物色素，并且通过将红色染色体变成PHYA缺陷拟南芥（生态型col- 0）。具有红移植物色素的转基因植物与野生型植物和具有野生型植物色素的转基因植物相比，显示明显增加的遮荫耐受性。因此，本发明可以用于抑制作物诱导作物产量损失的主要原因之一的植物避光，最终产生具有较高产量的耐荫植物。本发明还包括具有至少一个表达经修饰的PHYA的细胞的植物，包含至少一部分修饰的PHYA核酸的载体，以及使用这种载体产生具有阴性耐受性的植物的方法。

4. 发明授权

US07192775B1 Pathogenesis related protein and use thereof 有权
标题翻译：发病相关蛋白及其用途
公开(公告)号：US07192775B1
公开(公告)日：2007-03-20
申请号：US11249345
申请日：2005-10-14
申请人： Young Soon Kim , Ae Ran Park , Moon Kyung Ko , Jae Bok Yoon , Hyo Guen Park , Pill-Soon Song
发明人： Young Soon Kim , Ae Ran Park , Moon Kyung Ko , Jae Bok Yoon , Hyo Guen Park , Pill-Soon Song
IPC分类号： C12N15/82 , C12N5/10 , C12N15/74 , C07H21/04
CPC分类号： C07K14/415 , C12N15/8282
摘要： The invention relates to a gDNA and corresponding cDNA sequence comprising pathogenesis related protein-10 (CbPR-10) gene in Capsicum baccatum. The polypeptide encoded by the DNA sequence has ribonuclease activity which makes the enzyme highly effective in inhibiting the growth of fungal pathogen. The use of the DNA sequence disclosed herein contains the gDNA, cDNA, protein and a genetic construct for the production of transgenic plants, especially genetically transformed pepper plants, with an enhanced resistance against fungal pathogens.
摘要翻译：本发明涉及一种在辣椒中含有发病相关蛋白-10（CbPR-10）基因的gDNA和相应的cDNA序列。由DNA序列编码的多肽具有核糖核酸酶活性，这使酶在抑制真菌病原体的生长方面非常有效。本文公开的DNA序列的使用包含gDNA，cDNA，蛋白质和用于产生转基因植物，特别是经遗传转化的胡椒植物的遗传构建体，其对真菌病原体具有增强的抗性。

5. 发明授权

US07164013B2 Hyperactive light signal related molecule of HFR1-ΔN105 and transgenic plant thereof 失效
标题翻译： HFR1-DeltaN105的多活性光信号相关分子及其转基因植物
公开(公告)号：US07164013B2
公开(公告)日：2007-01-16
申请号：US10641100
申请日：2003-08-15
申请人： Moon-Soo Soh , Pill-Soon Song , Ki-Young Yang
发明人： Moon-Soo Soh , Pill-Soon Song , Ki-Young Yang
IPC分类号： C12N15/29 , C12N15/52
CPC分类号： C12N15/8237
摘要： This invention is about the functionally hyperactive light signal related molecule, HFR1-ΔN105, of which the nucleic acids that encode N-terminal 105 amino acid residues were deleted. HFR1 as a bHLH transcription factor functions in a subset of phytochrome A signaling cascade and it was reported to be regulated negatively by COP1. Experiments with a HFR1-ΔN105 overexpressing plant revealed that the deletion of N-terminal amino acids makes the HFR1 more active in photomorphogenic development such as germination and de-etiolation. In addition, the transgenic plants showed hypersensitive photo-responses in the inhibition of hypocotyl elongation, dependently on another positive element of light signaling, a bZIP protein, HY5. The end-of-day far-red light response and petiole elongation were suppressed in the HFR1-ΔN105 overexpressing plants. These results suggest that N-terminal region of HFR1 negatively regulate HFR1 function and that HFR1-ΔN105 is hyperactive.
摘要翻译：本发明涉及功能性超活动光信号相关分子HFR1-DeltaN105，其中编码N末端105个氨基酸残基的核酸被缺失。 HFR1作为bHLH转录因子在植物色素A信号级联的一个子集中起作用，据报道COP1负调节。 HFR1-DeltaN105过表达植物的实验显示，N末端氨基酸的缺失使得HFR1在光形态发生如萌发和脱乙酰化中更具活性。此外，转基因植物在抑制下胚轴延伸方面表现出过敏光响应，依赖于光信号传导的另一阳性元件bZIP蛋白HY5。在HFR1-DeltaN105过表达植物中，终末的远红光反应和叶柄伸长被抑制。这些结果表明，HFR1的N末端区域负调节HFR1功能，HFR1-DeltaN105是多活性的。

6. 发明授权

US07045680B2 Transgenic zoysiagrass with reduced shade avoidance 失效
标题翻译：具有减少遮荫避免的转基因动物学
公开(公告)号：US07045680B2
公开(公告)日：2006-05-16
申请号：US10268838
申请日：2002-10-11
申请人： Tohyama Kohichi , Jeon-Gu Kang , Hyo-Yeon Lee , Jeong-Il Kim , Pill-Soon Song
发明人： Tohyama Kohichi , Jeon-Gu Kang , Hyo-Yeon Lee , Jeong-Il Kim , Pill-Soon Song
IPC分类号： C12N15/82 , C12N15/90 , A01H5/00
CPC分类号： C12N15/8261 , C07K14/415 , Y02A40/146
摘要： The present invention provides transgenic zoysiagrass plant cells transformed with a modified oat phytochrome A gene (S598A) in which the serine-598 codon was replaced with an alanine codon, which, when expressed, significantly suppresses shade avoidance reactions, such as dwarfish internodes and petioles, short leaves, increased branching, and dark-green leaf color. Said transgenic zoysiagrass shows a robust appearance and is expected to show enhanced resistance to biotic and abiotic stresses. Also provided are a recombinant DNA construct comprising the S598A gene which, when expressed, induces drastic suppression of shade avoidance reactions, transgenic plants produced by this method, and propagating seeds obtained from said transgenic plants.
摘要翻译：本发明提供用经修饰的燕麦植物色素A基因（S598A）转化的转基因植物细胞植物细胞，其中丝氨酸-598密码子被丙氨酸密码子替代，当表达时，其显着抑制避光反应，例如fish鱼节间和叶柄，叶短，分枝增多，深绿色叶色。所述转基因动物学表现出强烈的外观，并且预期显示出对生物和非生物胁迫的增强的抗性。还提供了包含S598A基因的重组DNA构建体，当其表达时，其诱导阴性避免反应的剧烈抑制，通过该方法产生的转基因植物和繁殖从所述转基因植物获得的种子。

7. 发明申请

US20070136897A1 Nucleic acid molecules encoding hyperactive nucleoside di-phosphate kinase 2 and uses thereof 审中-公开
公开(公告)号：US20070136897A1
公开(公告)日：2007-06-14
申请号：US11247236
申请日：2005-10-12
申请人： Jeong-II Kim , Yun-Jeong Han , Seong-Hee Kim , Pill-Soon Song
发明人： Jeong-II Kim , Yun-Jeong Han , Seong-Hee Kim , Pill-Soon Song
IPC分类号： A01H1/00 , C07H21/04 , C12N5/04 , C12N15/82
CPC分类号： C12N9/1229 , C12N15/8261 , C12N15/8271 , Y02A40/146
摘要： The present invention includes modified Arabidopsis Nucleoside Di-Phosphate Kinase 2 (NDPK2) nucleic acid molecules whose enzymatic activity have been increased (i.e. hyperactive). NDPKs are ubiquitous housekeeping enzymes that catalyze the transfer of γ-phosphoryl group from a nucleoside triphosphate (NTP) to a nucleoside diphosphate (NDP), and also multifunctional proteins that regulate a variety of eukaryotic cellular activities, including cell proliferation, development, and differentiation. In plants, NDPKs are reported to play a key role in the signaling of both stress and light. Among three NDPKs (NDPK1, NDPK2, NDPK3) in a model plant, Arabidopsis thaliana, NDPK2 was reported as a positive signal transducer of phytochrome-mediated plant light signaling and to regulate cellular redox state, which enhances multiple stress tolerance in transgenic plants. Thus, the plants with the hyperactive NDPK2 are expected to possess higher efficiency of light utilization and enhanced tolerance to various environmental stresses such as cold, salt, and oxidative stresses. Since abiotic stress is one of the most important factors to limit the productivity of many crops, the hyperactive NDPK2 can be used for the development of high-yielding multiple stress tolerant plants with higher efficiency of light utilization. In this invention, several hyperactive NDPK2 were generated by C-terminal deletion and site-directed mutagenesis. Therefore, the present invention can be utilized to develop multiple stress tolerant and efficiently light-utilizing plants, which can eventually increase crop yields. The invention also includes plants having at least one cell expressing the modified NDPK2, vectors comprising at least one portion of the modified NDPK2 nucleic acids, and methods using such vectors for producing plants with enhanced light sensitivity and stress tolerance.

8. 发明授权

US5346083A Container and/or closure therefor 失效
标题翻译：集装箱和/或关闭
公开(公告)号：US5346083A
公开(公告)日：1994-09-13
申请号：US173280
申请日：1993-12-27
申请人： Pill-Soon Song , William R. Parker, Jr.
发明人： Pill-Soon Song , William R. Parker, Jr.
IPC分类号： B65D1/02 , B65D39/08 , B65D41/04 , B65D45/06 , B65D53/00
CPC分类号： B65D41/0414 , B65D1/023 , B65D39/08 , B65D45/06
摘要： To permit a container and a cap to remate with each other during resealing without fouling by solid debris such as small particles without exceptional preparations to withstand the deteriorating effects of corrosive materials and solvents and to consistently and quickly form a gas-tight seal, the container has a flat angled surface and the cap has cooperating edge that engages the flat surface in a closed line when the cap is fully inserted with a solid closed surface within the line, the closed line having a width perpendicular to its length within a range of 0.5 millimeters to 1.5 millimeters. The cap is closed by screw threads or the like that force the cooperating surfaces together with a pressure of at least 100 psi at an angle of between 75 degrees and 105 degrees.
摘要翻译：为了允许容器和盖子在重新密封期间彼此重新凝结，而不会由诸如小颗粒的固体碎屑污染而没有特殊的制剂以抵抗腐蚀性材料和溶剂的劣化作用并且始终如一地形成气密密封，容器具有平坦的倾斜表面，并且当盖完全插入线内的实心封闭表面时，盖具有与封闭线中的平坦表面接合的配合边缘，该闭合线具有垂直于其长度的宽度在0.5的范围内毫米至1.5毫米。帽被螺纹或类似物封闭，螺纹等将压配合表面以至少100psi的压力以75度和105度之间的角度相加。

9. 发明申请

US20060260009A1 Nucleic acid molecule encoding bathochromic phytochrome and use thereof 失效
标题翻译：编码红色色素植物色素的核酸分子及其用途
公开(公告)号：US20060260009A1
公开(公告)日：2006-11-16
申请号：US11129459
申请日：2005-05-16
申请人： Jeong-Il Kim , Yun-Jeong Han , Pill-Soon Song
发明人： Jeong-Il Kim , Yun-Jeong Han , Pill-Soon Song
IPC分类号： A01H1/00 , C07H21/04 , C12N15/82
CPC分类号： C12N15/8261 , C07K14/415 , Y02A40/146
摘要： The present invention includes modified phytochrome A (PHYA) nucleic acid molecules in which Pr absorption spectra have been shifted to longer wavelength (i.e. bathochromism or red-shift). The plants with the bathochromic phytochromes are expected to respond to canopy and shade conditions for growth and development with greater efficiency than the plants with wild-type phytochrome (i.e. suppression of shade avoidance reactions in plants). Since the shade avoidance reactions in plants induce a rapid and dramatic increase in the extension growth of stems and petioles at the expense of leaf growth, storage organ production, and reproductive development, it causes significant losses of crop yields. Thus, the bathochromic phytochromes that utilize the shade light efficiently would suppress the shade avoidance reactions in plants, giving plants the tolerance to shade. In this invention, several bathochromic phytochromes were generated by site-directed mutagenesis in the region of bilin lyase domain in plant PHYA, and their ability to suppress the shade avoidance reactions were examined by transforming the bathochromic phytochromes into PHYA deficient Arabidopsis thaliana (ecotype col-0). The transgenic plants with the bathochromic phytochromes showed significantly increased shade tolerance compared to wild-type plants and transgenic plants with wild-type phytochromes. Therefore, the present invention can be utilized to suppress plants' shade avoidance that is one of major causes to induce crop-yield losses, and ultimately to generate shade tolerant plants with higher yields. The invention also includes plants having at least one cell expressing the modified PHYA, vectors comprising at least one portion of the modified PHYA nucleic acids, and methods using such vectors for producing plants with shade tolerance.
摘要翻译：本发明包括修饰的植物色素A（PHYA）核酸分子，其中Pr吸收光谱已经转移到更长的波长（即，红移或红移）。具有红色色素植物色素的植物预期对具有野生型植物色素的植物（即抑制植物中的树荫回避反应）的效率比用于生长和发育的冠层和阴凉条件更有效。由于植物中的避光反应以叶片生长，储存器官生产和繁殖发育为代价诱导茎和叶柄的延伸生长快速和显着地增加，导致作物产量的显着损失。因此，有效利用遮光效果的红色植物色素可以抑制植物的避光反应，使植物具有遮荫性。在本发明中，通过在植物PHYA中的胆固醇裂解酶结构域中的定点诱变产生了几种红色植物色素，并且通过将红色染色体变成PHYA缺陷拟南芥（生态型col- 0）。具有红移植物色素的转基因植物与野生型植物和具有野生型植物色素的转基因植物相比，显示明显增加的遮荫耐受性。因此，本发明可以用于抑制作物诱导作物产量损失的主要原因之一的植物避光，最终产生具有较高产量的耐荫植物。本发明还包括具有至少一个表达经修饰的PHYA的细胞的植物，包含至少一部分修饰的PHYA核酸的载体，以及使用这种载体产生具有阴性耐受性的植物的方法。

10. 发明授权

US06946294B2 Transgenic plant transformed with a translationally controlled tumor protein (TCTP) gene 失效
标题翻译：用转移控制的肿瘤蛋白（TCTP）基因转化的转基因植物
公开(公告)号：US06946294B2
公开(公告)日：2005-09-20
申请号：US10345599
申请日：2003-01-16
申请人： Jeong-Gu Kang , Ju Yun , Pill-Soon Song , Chung-Mo Park
发明人： Jeong-Gu Kang , Ju Yun , Pill-Soon Song , Chung-Mo Park
IPC分类号： C12N5/06 , C07K14/415 , C12N15/82 , C12N5/10
CPC分类号： C12N15/8261 , C07K14/415 , Y02A40/146
摘要： The present invention provides transgenic higher plants and transgenic plant cells thereof which have been transformed with a plant translationally controlled tumor protein (TCTP) gene to improve growth rate in a growth stage-dependent manner, especially in the earlier vegetative growth stage from seedlings to young plants. Also, the invention provides methods for engineering higher plants so that they can be more efficiently transformed with other transforming vector constructs. Further, provided are TCTP expression vectors which contain an uninterrupted coding region for the TCTP protein used to generate transgenic plants and cells thereof.
摘要翻译：本发明提供已经用植物翻译控制的肿瘤蛋白（TCTP）基因转化的转基因高等植物和转基因植物细胞，以改善生长阶段依赖性的生长速率，特别是在从幼苗到幼年的早期营养生长阶段植物。此外，本发明提供了用于工程化高等植物以使其可以用其他转化载体构建体更有效地转化的方法。此外，提供了TCTP表达载体，其含有用于产生转基因植物及其细胞的TCTP蛋白的不间断编码区。

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